植物生态学报 ›› 2016, Vol. 40 ›› Issue (9): 933-941.DOI: 10.17521/cjpe.2015.0261
收稿日期:
2015-07-08
接受日期:
2016-04-25
出版日期:
2016-09-10
发布日期:
2016-09-29
通讯作者:
孙雪
基金资助:
Jia SHEN, Ya-He LI, Lin ZHANG, Xue SUN*()
Received:
2015-07-08
Accepted:
2016-04-25
Online:
2016-09-10
Published:
2016-09-29
Contact:
Xue SUN
摘要:
为了探讨光照强度和CO2浓度对蛋白核小球藻(Chlorella pyrenoidosa)生长、无机碳利用的复合效应, 丰富绿藻中无机碳浓缩机制的资料, 该文设置两种光照强度(40和120 µmol photons•m-2•s-1)和两种CO2浓度(0.04%和0.16%)组合成4种条件, 比较了蛋白核小球藻生长、无机碳浓度、pH补偿点、光合放氧速率、碳酸酐酶(CA)活性和α-CA基因转录表达对这4种培养条件的响应。结果发现: 蛋白核小球藻在高光强高CO2浓度组生长最快; 低光强高CO2浓度组培养体系中总无机碳浓度为1163.3 µmol•L-1, 显著高于其他3组; 高光强低CO2浓度组藻的pH补偿点最高(9.8), 而低光强高CO2浓度组藻的pH补偿点最低(8.6); 低光强高CO2浓度组藻的最大光合速率(Vmax)和最大光合速率一半时的无机碳浓度(K0.5)最高, 分别是其他3组的1.28-1.91倍和1.61-2.00倍; 高光强低CO2浓度组藻的胞外CA活性最高; 而低光强低CO2浓度组藻的胞外α-CA基因表达量显著高于其他3组。以上结果表明低CO2浓度可促进蛋白核小球藻的pH补偿点和无机碳亲和力的提高, 诱导胞外CA活性及α-CA基因的表达; 该藻主要以HCO3-为无机碳源, 其对无机碳的利用受光照的调节。
沈佳, 李亚鹤, 张琳, 孙雪. 蛋白核小球藻生长和无机碳利用对不同光照强度和CO2浓度的响应. 植物生态学报, 2016, 40(9): 933-941. DOI: 10.17521/cjpe.2015.0261
Jia SHEN, Ya-He LI, Lin ZHANG, Xue SUN. Response of growth and inorganic carbon utilization to different light and CO2 levels in Chlorella pyrenoidosa. Chinese Journal of Plant Ecology, 2016, 40(9): 933-941. DOI: 10.17521/cjpe.2015.0261
图1 不同光照强度和CO2浓度对蛋白核小球藻生长的影响(平均值±标准偏差)。A, 生长曲线。B, 比生长速率。LL、LH、HL和HH分别表示低光强低CO2浓度、低光强高CO2浓度、高光强低CO2浓度、高光强高CO2浓度条件。不同小写字母表示差异显著(p < 0.05)。
Fig. 1 Effect of different light intensity and CO2 concentration conditions on the growth of Chlorella pyrenoidosa (mean ± SD). A, Growth curve. B, Specific growth rate. LL, LH, HL and HH represent low-light intensity and low-CO2 concentration, low-light intensity and high-CO2 concentration, high-light intensity and low-CO2 concentration, high-light intensity and high-CO2 concentration conditions, respectively. Different lowercase letters indicate significant difference (p < 0.05).
处理组 Treatment group | 总碱度 Total alkalinity (µmol•L-1) | pH | DIC (µmol•L-1) | CO2 (µmol•L-1) | HCO3- (µmol•L-1) | CO32- (µmol•L-1) |
---|---|---|---|---|---|---|
LL | 1 387.8 ± 55.1b | 9.35 ± 0.00c | 765.3 ± 36.8ab | 0.22 ± 0.18a | 383.6 ± 18.44a | 381.5 ± 18.3c |
LH | 1 388.4 ± 57.3b | 8.48 ± 0.03a | 1 163.3 ± 58.8c | 4.37 ± 0.46b | 1 022.1 ± 57.4c | 136.8 ± 3.6a |
HL | 1 275.0 ± 16.5ab | 9.41 ± 0.04c | 665.8 ± 22.6a | 0.16 ± 0.44a | 311.7 ± 24.2a | 354.0 ± 5.8c |
HH | 1 190.1 ± 0.0a | 8.95 ± 0.04b | 803.4 ± 17.2b | 0.84 ± 0.12a | 574.9 ± 27.2b | 227.6 ± 10.2b |
表1 不同光照强度和CO2浓度条件下蛋白核小球藻培养体系中各碳酸盐参数的比较(平均值±标准偏差, n = 3)
Table 1 Comparison of the parameters of carbonate system under different light intensity and CO2 concentration conditions in Chlorella pyrenoidosa (mean ± SD, n = 3)
处理组 Treatment group | 总碱度 Total alkalinity (µmol•L-1) | pH | DIC (µmol•L-1) | CO2 (µmol•L-1) | HCO3- (µmol•L-1) | CO32- (µmol•L-1) |
---|---|---|---|---|---|---|
LL | 1 387.8 ± 55.1b | 9.35 ± 0.00c | 765.3 ± 36.8ab | 0.22 ± 0.18a | 383.6 ± 18.44a | 381.5 ± 18.3c |
LH | 1 388.4 ± 57.3b | 8.48 ± 0.03a | 1 163.3 ± 58.8c | 4.37 ± 0.46b | 1 022.1 ± 57.4c | 136.8 ± 3.6a |
HL | 1 275.0 ± 16.5ab | 9.41 ± 0.04c | 665.8 ± 22.6a | 0.16 ± 0.44a | 311.7 ± 24.2a | 354.0 ± 5.8c |
HH | 1 190.1 ± 0.0a | 8.95 ± 0.04b | 803.4 ± 17.2b | 0.84 ± 0.12a | 574.9 ± 27.2b | 227.6 ± 10.2b |
图2 不同光照强度和CO2浓度下蛋白核小球藻的pH补偿点(平均值±标准偏差)。LL、LH、HL和HH分别表示低光强低CO2浓度、低光强高CO2浓度、高光强低CO2浓度、高光强高CO2浓度条件。不同小写字母表示差异显著(p < 0.05)。
Fig. 2 Effect of different light intensity and CO2 concentration conditions on pH compensation point of Chlorella pyrenoidosa (mean ± SD). LL, LH, HL and HH represent low-light intensity and low-CO2 concentration, low-light intensity and high-CO2 concentration, high-light intensity and low-CO2 concentration, high-light intensity and high-CO2 concentration conditions, respectively. Different lowercase letters indicate significant difference (p < 0.05).
图3 不同光照强度和CO2浓度下蛋白核小球藻的P-C曲线(平均值±标准偏差)。LL、LH、HL和HH分别表示低光强低CO2浓度、低光强高CO2浓度、高光强低CO2浓度、高光强高CO2浓度条件。不同小写字母表示差异显著(p < 0.05)。
Fig. 3 Effect of different light intensity and CO2 concentration conditions on P-C curve of Chlorella pyrenoidosa (mean ± SD). LL, LH, HL and HH represent low-light intensity and low-CO2 concentration, low-light intensity and high-CO2 concentration, high-light intensity and low-CO2 concentration, high-light intensity and high-CO2 concentration conditions, respectively. Different lowercase letters indicate significant difference (p < 0.05).
处理组 Treatmeat group | Vmax (µmol O2• 108cell·h-1) | K0.5 (µmol•L-1) | ||
---|---|---|---|---|
DIC | CO2 | HCO3- | ||
LL | 275.93 ± 16.83b | 107.20 ± 2.49a | 2.43 ± 0.06a | 104.78 ± 2.43a |
LH | 415.44 ± 4.23d | 188.33 ± 2.04b | 4.26 ± 0.45b | 184.07 ± 19.59b |
HL | 218.03 ± 10.16a | 94.20 ± 7.03a | 2.13 ± 0.16a | 92.07 ± 6.87a |
HH | 324.05 ± 1.34c | 116.83 ± 6.89a | 2.64 ± 0.16a | 114.19 ± 6.73a |
表2 不同光照强度和CO2浓度下蛋白核小球藻的Vmax和K0.5 (平均值±标准偏差, n = 3)
Table 2 Effect of different light intensity and CO2 concentration conditions on Vmax and K0.5 of Chlorella pyrenoidosa (mean ± SD, n = 3)
处理组 Treatmeat group | Vmax (µmol O2• 108cell·h-1) | K0.5 (µmol•L-1) | ||
---|---|---|---|---|
DIC | CO2 | HCO3- | ||
LL | 275.93 ± 16.83b | 107.20 ± 2.49a | 2.43 ± 0.06a | 104.78 ± 2.43a |
LH | 415.44 ± 4.23d | 188.33 ± 2.04b | 4.26 ± 0.45b | 184.07 ± 19.59b |
HL | 218.03 ± 10.16a | 94.20 ± 7.03a | 2.13 ± 0.16a | 92.07 ± 6.87a |
HH | 324.05 ± 1.34c | 116.83 ± 6.89a | 2.64 ± 0.16a | 114.19 ± 6.73a |
图4 不同光照强度和CO2浓度下蛋白核小球藻的碳酸酐酶(CA)活性(平均值±标准偏差)。LL、LH、HL和HH分别表示低光强低CO2浓度、低光强高CO2浓度、高光强低CO2浓度、高光强高CO2浓度条件。不同字母表示差异显著(p < 0.05)。
Fig. 4 Effect of different light intensity and CO2 concentration conditions on carbonic anhydrase (CA) activity of Chlorella pyrenoidosa (mean ± SD). LL, LH, HL and HH represent low-light intensity and low-CO2 concentration, low-light intensity and high-CO2 concentration, high-light intensity and low-CO2 concentration, high-light intensity and high-CO2 concentration conditions, respectively. Different letters indicate significant difference (p < 0.05).
图5 不同光照强度和CO2浓度对蛋白核小球藻α-CA基因表达的影响(平均值±标准偏差)。LL、LH、HL和HH分别表示低光强低CO2浓度、低光强高CO2浓度、高光强低CO2浓度、高光强高CO2浓度条件。不同小写字母表示差异显著(p < 0.05)。
Fig. 5 The effect of different light intensity and CO2 concentration conditions on α-CA gene expression of Chlorella pyrenoidosa (mean ± SD). LL, LH, HL and HH represent low-light intensity and low-CO2 concentration, low-light intensity and high-CO2 concentration, high-light intensity and low-CO2 concentration, high-light intensity and high-CO2 concentration conditions, respectively. Different lowercase letters indicate significant difference (p < 0.05).
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