分次追施N肥对苹果砧木—平邑甜茶吸收 15N-尿素以及叶片衰老的影响

doi:10.3724/SP.J.1258.2012.01286

摘要/Abstract

摘要：

以盆栽平邑甜茶(Malus hupenhensis)为实验材料, 研究等氮(N)量分次追施N肥(一次、二次和三次)对平邑甜茶叶片衰老及 ¹⁵N-尿素吸收、利用的影响。采用 ¹⁵N示踪技术, 研究不同施肥处理下植株的生长、酶活性和 ¹⁵N吸收利用等参数。研究结果表明: 植株的株高、茎粗、叶面积和叶绿素含量(SPAD)在生长前期均以一次性追肥处理最高, 三次追肥处理最低, 且与一次追肥处理差异显著; 在生长中期均以二次追肥处理最高, 一次追肥处理最低; 在生长后期均以三次追肥处理最高, 一次追肥处理最低, 且与三次追肥处理差异显著; 叶片的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性在生长前期均以一次追肥处理最高, 三次追肥处理最低, 且与一次追肥处理差异显著; 在生长中期均以二次追肥处理最高, 一次追肥处理最低; 在生长后期均以三次追肥处理最高, 一次追肥处理最低, 且与三次追肥处理差异显著; 生长后期植株各器官从肥料中吸收分配到的 ¹⁵N量对该器官全N量的贡献率差异显著, 三次追肥处理显著高于一次和二次追肥处理; 生长后期三次追肥处理植株的总N量、吸收的 ¹⁵N量及 ¹⁵N肥料利用率均为最大。三次追肥处理能提高叶片全N量, 延缓生长后期叶片衰老, 提高N肥利用率。

关键词: 吸收, 分次追肥, 叶片衰老, 平邑甜茶, ¹⁵N标记的尿素, 利用

Abstract:

Aims The effects of fractionated nitrogen (N) fertilization (once, twice and thrice) on leaf senescence and nitrogen uptake and utilization of potted Malus hupenhensis were explored using the ¹⁵N labeling technique. Methods We measured growth parameters such as plant height, stem diameter, leaf area and chlorophyll content (SPAD), enzyme parameters such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in leaves and ¹⁵N parameters such as uptake and utilization for potted M. hupehensis under different fractionated N applications. Important findings Plant height, stem diameter, leaf area and SPAD were highest at early growth stage for once fractionated application and lower for twice fractionated application, and lowest for thrice fractionated application. At middle growth stage, twice fractionated application showed the highest values, while once fractionated application exhibited the lowest values. At the late growth stage, thrice fractionated application was the highest, while once fractionated application was the lowest. The leaf SOD, POD and CAT activities were highest for once fractionated application at early growth stage and lowest for thrice fractionated application. At the middle growth stage, twice fractionated application was the highest, whereas once fractionated application was lowest. At the late growth stage, thrice fractionated application showed the highest values, while once fractionated application had the lowest values. The ¹⁵N derived from fertilizer (N_dff) value in different organs was significantly different. Thrice fractionated application demonstrated higher values than either of once and twice fractionated application at the late growth stage. Plant total N, ¹⁵N uptake and N-usage efficiency were highest for thrice fractionated application at late growth stage. This indicates that thrice fractionated application not only can enhance the total N content of leaves and delay leaf senescence, but also can increase the N-usage efficiency.

Key words: absorption, fractionated application, leaf senescence, Malus hupenhensis, ¹⁵N-labeled urea, utilization

丁宁, 姜远茂, 魏绍冲, 陈倩, 葛顺峰. 分次追施N肥对苹果砧木—平邑甜茶吸收 ¹⁵N-尿素以及叶片衰老的影响. 植物生态学报, 2012, 36(12): 1286-1292. DOI: 10.3724/SP.J.1258.2012.01286

DING Ning, JIANG Yuan-Mao, WEI Shao-Chong, CHEN Qian, GE Shun-Feng. Effects of fractionated nitrogen application on leaf senescence and ¹⁵N-urea uptake and utilization of apple rootstock―Malus hupenhensis. Chinese Journal of Plant Ecology, 2012, 36(12): 1286-1292. DOI: 10.3724/SP.J.1258.2012.01286

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https://www.plant-ecology.com/CN/Y2012/V36/I12/1286

图/表 5

参考文献 24

1	Bao SD ( 鲍士旦 ) (2000). Soil Agricultural-Chemical Analysis (土壤农化分析) 3rd edn. China Agriculture Press, Beijing. (in Chinese)
2	de Jong TM, Day KR, Johnson RS ( 1989). Partitioning of leaf nitrogen with respect to within canopy light exposure and nitrogen availability in peach. Trees, 3, 89-95. DOI URL
3	Fang XJ ( 房祥吉), Jiang YM ( 姜远茂), Peng FT ( 彭福田 ) ( 2011). Effects of different sand/soil ratio on growth and ¹⁵N absorption, utilization and loss of potted Malus hupenhensis. Journal of Soil and Water Conservation (水土保持学报), 25(4), 131-134. (in Chinese with English abstract)
4	Gao XL ( 高小丽), Sun JM ( 孙健敏), Gao JF ( 高金峰), Feng BL ( 冯佰利), Chai Y ( 柴岩), Jia ZK ( 贾志宽 ) ( 2008). Leaf aging and reactive oxygen metabolism in different genotypes of mung bean. Scientia Agricultura Sinica (中国农业科学), 41, 2873-2880. (in Chinese with English abstract)
5	Gou L ( 勾玲), Yan J ( 闫洁), Han CL ( 韩春丽 ) ( 2004). Effects of nitrogen rates on photosynthetic characteristics and yield of high-yielding cotton in Xinjiang. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 10, 488-493. (in Chinese with English abstract)
6	Gu MR ( 顾曼如 ) ( 1990). The application of ¹⁵N in the study of nitrogen nutrition on apples . China Fruits (中国果树), ( 2), 46-48. (in Chinese with English abstract)
7	Gu MR ( 顾曼如), Zhang RZ ( 张若杼), Shu HR ( 束怀瑞), Li WW ( 黎文文), Huang HC ( 黄花成 ) ( 1981). A study on the nitrogen nutrition of apple trees—the variation of nitrogen nutrition within the plant in a year’s cycle. Acta Horticulture Sinica (园艺学报), 8(4), 21-28. (in Chinese with English abstract)
8	Guo G L ( 郭改玲), Liu KL ( 刘克礼), Gao JL ( 高聚林 ) ( 2006). Effect of nitrogen application on flag leaf senescence and yield of spring wheat. Journal of Triticeae Crops (麦类作物学报), 26(5), 126-129. (in Chinese with English abstract)
9	Han ZH ( 韩振海), Zeng X ( 曾骧), Wang FJ ( 王福钧 ) ( 1992). Effects of autumn foliar application of ¹⁵N-urea and several PGR on N storage and reuse in apple . Acta Horticulture Sinica (园艺学报), 19, 15-21. (in Chinese with English abstract)
10	Hobbie L, Estelle M ( 1994). Genetic approaches to auxin action. Plant, Cell & Environment, 17, 525-540.
11	Jiang YM ( 姜远茂), Peng FT ( 彭福田), Zhang HY ( 张宏彦), Li XL ( 李晓林), Zhang FS ( 张福锁 ) ( 2001). Status of organic matter and nutrients in Shandong orchard soil. Chinese Journal of Soil Science (土壤通报), 32(4), 167-169. (in Chinese with English abstract)
12	Khemira H ( 1991). Influence of Canopy Orientation on Fruiting of ‘Anjou’ Pears and Post Harvest Urea Spray on Ovule Longevity and Fruit Set of ‘Comice’ Pears. Master degree dissertation, Oregon State University, Corvallis.
13	Li WQ ( 李文庆), Zhang M ( 张民), Shu HR ( 束怀瑞 ) ( 2002). The physiological effects of nitrogen on fruit trees. Journal of Shandong Agricultural University (Natural Science) (山东农业大学学报(自然科学版)), 33, 96-100. (in Chinese with English abstract)
14	Lin ZF ( 林植芳), Li SS ( 李双顺), Lin GZ ( 林桂珠), Sun GC ( 孙谷畴), Guo JY ( 郭俊彦 ) ( 1984). Superoxide dismutase activity and lipid peroxidation in relation to senescence of rice leaves. Acta Botanica Sinica (植物学报), 26, 605-615. (in Chinese with English abstract)
15	Oaks A, Aslam M, Boesel Z ( 1997). Ammonium and amino acid as regulators of nitrate reductase in corn roots. Plant Physiology, 59, 391-394.
16	Peng FT ( 彭福田), Jiang YM ( 姜远茂), Gu MR ( 顾曼如), Shu HR ( 束怀瑞 ) ( 2003). Effect of nitrogen on apple fruit hormone changing trends and development. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 9, 208-213. (in Chinese with English abstract)
17	Peng FT ( 彭福田), Zhang Q ( 张青), Jiang YM ( 姜远茂), Peng Y ( 彭勇), Zhou P ( 周鹏 ) ( 2006). Effect of nitrogen application on nitrogen absorption, distribution and yield of strawberry. Plant Nutrition and Fertilizer Science (植物营养与肥料学报), 12, 400-405. (in Chinese with English abstract)
18	Raese JT, Drake SR, Curry EA ( 2007). Nitrogen fertilizer influences fruit quality, soil nutrients and cover crops, leaf color and nitrogen content, biennial bearing and cold hardiness of “Golden Delicious”. Journal of Plant Nutrition, 30, 1585-1604.
19	Shi CY ( 史沉鱼), Li XM ( 李向民), Li XD ( 李晓东), Zhang R ( 张蓉 ) ( 2010). Two fertilizer treatments on the aroma components in Fuji apples. Northern Horticulture (北方园艺), ( 3), 1-3. (in Chinese with English abstract)
20	Stassen PJC, Terblanche JH, Strydom DK ( 1981). The effect of time and rate of nitrogen application on development and composition of peach trees. Agoplantae, 13, 55-61.
21	Wang AG ( 王爱国), Luo GH ( 罗广华), Shao CB ( 邵从本), Wu SJ ( 吴淑君), Guo JY ( 郭俊彦 ) ( 1983). A study on the superoxide dismutase of soybean seeds. Acta Photophysiologica Sinica (植物生理学报), 9, 77-84. (in Chinese with English abstract)
22	Wang XY ( 王晓云), Li XD ( 李向东), Zou Q ( 邹琦 ) ( 2001). Regulation effects of nitrogen application on the polyamine metabolism and senescence of peanut leaves. Acta Agronomica Sinica (作物学报), 27, 442-446. (in Chinese with English abstract)
23	Wei HY ( 魏海燕), Zhang HC ( 张洪程), Ma Q ( 马群 ) ( 2010). Characteristics of leaf senescence in rice genotypes with different nitrogen use efficiencies. Acta Agronomica Sinica (作物学报), 36, 645-654. (in Chinese with English abstract)
24	Xu YX ( 徐艳霞), Wei ZB ( 魏占彬), Wang TL ( 王天亮 ) ( 2009). Effect of different fertilization methods on growth and grain yield of summer maize. Anhui Agricultural Science Bulletin (安徽农学通报), 15(18), 54-55. (in Chinese with English abstract)

项目 Item	生长前期 Early growth stage						生长后期 Late growth stage
项目 Item	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application
株高 Plant height (cm)	11.98 ± 0.66^a	10.80 ± 0.45^b	10.05 ± 0.29^b	19.58 ± 0.49^b	22.04 ± 0.44^a	20.58 ± 0.69^b	26.49 ± 0.73^b	27.62 ± 0.69^ab	28.37 ± 0.76^a
茎粗 Stem diameter (mm)	2.57 ± 0.01^a	2.54 ± 0.01^b	2.53 ± 0.01^b	2.79 ± 0.04^b	2.91 ± 0.03^a	2.87 ± 0.03^a	3.14 ± 0.05^c	3.28 ± 0.03^b	3.52 ± 0.08^a
叶面积 Leaf area (cm²)	8.94 ± 0.29^a	8.33 ± 0.04^b	8.19 ± 0.06^b	12.58 ± 0.31^c	13.97 ± 0.25^a	13.42 ± 0.23^b	16.22 ± 0.57^b	17.54 ± 0.69^b	19.73 ± 0.96^a
叶绿素含量 Chlorophyll content (SPAD)	51.70 ± 1.61^a	46.43 ± 1.08^b	43.63 ± 0.95^c	53.23 ± 1.01^b	57.03 ± 1.04^a	54.20 ± 1.55^ab	44.57 ± 1.51^c	48.81 ± 1.18^b	53.57 ± 1.27^a
叶片全N量 Total N content of leaves (g·plant^-1)	0.06 ± 0.03^a	0.05 ± 0.02^a	0.05 ± 0.03^a	0.08 ± 0.01^a	0.09 ± 0.01^a	0.09 ± 0.01^a	0.06 ± 0.00^b	0.07 ± 0.01^b	0.09 ± 0.00^a

项目 Item	生长前期 Early growth stage						生长后期 Late growth stage
项目 Item	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application
株高 Plant height (cm)	11.98 ± 0.66^a	10.80 ± 0.45^b	10.05 ± 0.29^b	19.58 ± 0.49^b	22.04 ± 0.44^a	20.58 ± 0.69^b	26.49 ± 0.73^b	27.62 ± 0.69^ab	28.37 ± 0.76^a
茎粗 Stem diameter (mm)	2.57 ± 0.01^a	2.54 ± 0.01^b	2.53 ± 0.01^b	2.79 ± 0.04^b	2.91 ± 0.03^a	2.87 ± 0.03^a	3.14 ± 0.05^c	3.28 ± 0.03^b	3.52 ± 0.08^a
叶面积 Leaf area (cm²)	8.94 ± 0.29^a	8.33 ± 0.04^b	8.19 ± 0.06^b	12.58 ± 0.31^c	13.97 ± 0.25^a	13.42 ± 0.23^b	16.22 ± 0.57^b	17.54 ± 0.69^b	19.73 ± 0.96^a
叶绿素含量 Chlorophyll content (SPAD)	51.70 ± 1.61^a	46.43 ± 1.08^b	43.63 ± 0.95^c	53.23 ± 1.01^b	57.03 ± 1.04^a	54.20 ± 1.55^ab	44.57 ± 1.51^c	48.81 ± 1.18^b	53.57 ± 1.27^a
叶片全N量 Total N content of leaves (g·plant^-1)	0.06 ± 0.03^a	0.05 ± 0.02^a	0.05 ± 0.03^a	0.08 ± 0.01^a	0.09 ± 0.01^a	0.09 ± 0.01^a	0.06 ± 0.00^b	0.07 ± 0.01^b	0.09 ± 0.00^a

项目 Items	生长初期 Early growth stage			生长中期 Medium growth stage			生长后期 Late growth stage
项目 Items	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application
SOD (U·mg^-1FW)	5.15 ± 0.07^a	4.88 ± 0.06^b	4.59 ± 0.15^c	4.38 ± 0.06^b	4.58 ± 0.05^a	4.29 ± 0.06^b	2.56 ± 0.42^c	3.13 ± 0.14^b	3.77 ± 0.16^a
POD (U·mg^-1FW)	56.48 ± 5.63^a	51.49 ± 5.44a^b	42.56 ± 4.02^b	35.32 ± 2.13^c	44.45 ± 1.41^b	38.93 ± 1.74^b	12.75 ± 1.43^b	15.76 ± 1.70^ab	18.69 ± 1.63^a
CAT (U·mg^-1FW)	23.89 ± 1.87^a	18.35 ± 0.76^b	16.73 ± 1.08^b	4.73 ± 0.21^c	6.69 ± 0.28^a	5.73 ± 0.75^b	3.63 ± 0.22^b	3.85 ± 0.03^b	4.56 ± 0.39^a

项目 Items	生长初期 Early growth stage			生长中期 Medium growth stage			生长后期 Late growth stage
项目 Items	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application
SOD (U·mg^-1FW)	5.15 ± 0.07^a	4.88 ± 0.06^b	4.59 ± 0.15^c	4.38 ± 0.06^b	4.58 ± 0.05^a	4.29 ± 0.06^b	2.56 ± 0.42^c	3.13 ± 0.14^b	3.77 ± 0.16^a
POD (U·mg^-1FW)	56.48 ± 5.63^a	51.49 ± 5.44a^b	42.56 ± 4.02^b	35.32 ± 2.13^c	44.45 ± 1.41^b	38.93 ± 1.74^b	12.75 ± 1.43^b	15.76 ± 1.70^ab	18.69 ± 1.63^a
CAT (U·mg^-1FW)	23.89 ± 1.87^a	18.35 ± 0.76^b	16.73 ± 1.08^b	4.73 ± 0.21^c	6.69 ± 0.28^a	5.73 ± 0.75^b	3.63 ± 0.22^b	3.85 ± 0.03^b	4.56 ± 0.39^a

器官 Organs	追肥方式 Fertilization mode
器官 Organs	一次追肥 Once fractionated application	二次追肥 Twice fractionated application	三次追肥 Thrice fractionated application
根 Root	11.79 ± 0.31^b	12.31 ± 0.38^ab	13.86 ± 1.38^a
茎 Stem	10.79 ± 0.40^b	11.29 ± 0.41^ab	11.96 ± 0.17^a
叶 Leaf	8.85 ± 0.53^c	10.48 ± 0.67^b	11.69 ± 0.14^a

分次追施N肥对苹果砧木—平邑甜茶吸收 ¹⁵N-尿素以及叶片衰老的影响

Effects of fractionated nitrogen application on leaf senescence and ¹⁵N-urea uptake and utilization of apple rootstock―Malus hupenhensis

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追肥方式 Fertilization mode	植株总N量 Total N content of plant (g)	吸收的¹⁵N量 ¹⁵N absorbed from ¹⁵N-urea (g)	¹⁵N肥料利用率(%) ¹⁵N-urea utilization rate
一次追肥 Once fractionated application	0.12 ± 0.006 5^b	0.012 ± 0.000 4^b	4.12 ± 0.14^c
二次追肥 Twice fractionated application	0.13 ± 0.008 5^ab	0.015 ± 0.000 7^a	5.13 ± 0.23^b
三次追肥 Thrice fractionated application	0.14 ± 0.007 9^a	0.017 ± 0.001 7^a	6.01 ± 0.29^a

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