Effect of inoculation time on camptothecin content in arbuscular mycorrhizal Camptotheca acuminata seedlings

doi:10.3773/j.issn.1005-264x.2010.06.008

Abstract

Abstract:

Aims Arbuscular mycorrhizal fungi can directly or indirectly affect plant secondary metabolic processes. Camptothecin, a secondary metabolite in a special Chinese tree Camptotheca acuminata, has gained great attention for its remarkable inhibitory activity against tumor cells. The effect of two arbuscular mycorrhizal fungi belonging to two genera on the accumulation of camptothecin at different growth times of C. acuminata seedling was carried out in the present study.

Methods The selected sterile seeds of C. acuminata were sown in sterilized matrix (a mixture of soil and sand) in the greenhouse. Seedlings with similar height and crown were selected and divided into three groups (30 pots per group). Each group of C. acuminata seedlings was inoculated with a species of arbuscular mycorrhizal fungi Acaulospora mellea or Glomus intraradices, or non-mycorrhizal inoculation when seedlings unearthed 20 days, 40 days and 60 days, respectively. After 30 days of treatment, camptothecin contents and yields in the seedlings of C. acuminata were determined.

Important findings All inoculated seedlings were infected by arbuscular mycorrhizal fungi and formed arbuscular mycorrhiza after 30 days of co-cultivation. The results showed that camptothecin yields (camptothecin contents multiplied by biomass) in mycorrhizal seedlings were significantly higher than non-mycorrhizal seedlings. Furthermore, the increase of camptothecin yields in mycorrhizal seedlings was mainly credited to the increase of camptothecin contents in seedlings (especially in leaves) inoculated at early stage (20 days of seedlings emergence) or to the increase of plant biomass in seedlings inoculated at late stage (60 days of seedlings emergence).

Key words: arbuscular mycorrhiza, Camptotheca acuminata seedlings, camptothecin

YU Yang, YU Tao, WANG Yang, YAN Xiu-Feng. Effect of inoculation time on camptothecin content in arbuscular mycorrhizal Camptotheca acuminata seedlings[J]. Chin J Plant Ecol, 2010, 34(6): 687-694.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.plant-ecology.com/EN/10.3773/j.issn.1005-264x.2010.06.008

https://www.plant-ecology.com/EN/Y2010/V34/I6/687

Figures/Tables 5

Table 1 Effect of inoculation time on the infection of Camptotheca acuminata seedlings with arbuscular mycorrhizal fungi (mean ± SE)

接种时间 Inoculation time (幼苗出土天数 Days of seedlings emergence)	菌根侵染率 Mycorrhizal colonization rate (%)			根系的菌根侵染强度 Mycorrhizal colonization intensity of root (%)
接种时间 Inoculation time (幼苗出土天数 Days of seedlings emergence)	CK	Am	Gi		CK	Am	Gi
20	0	41.67 ± 2.58^a	29.41 ± 3.39^a	0		21.47 ± 0.32^a	4.44 ± 0.13^a
40	0	61.11 ± 3.17^b	22.00 ± 1.51^b	0		30.25 ± 0.41^b	1.36 ± 0.11^b
60	0	35.42 ± 2.29^c	20.00 ± 2.34^b	0		14.10 ± 0.29^c	1.00 ± 0.09^c

Fig. 1 Effect of inoculation time on biomass in arbuscular mycorrhizal Camptotheca acuminata seedlings. Am, CK, Gi, see Table1. In each panel, the columns with different letters are significantly different (p < 0.05).

Fig. 2 Effect of inoculation time on camptothecin content in arbuscular mycorrhizal Camptotheca acuminata seedlings. Notes see Fig. 1.

Fig. 3 Effect of inoculation time on camptothecin yield in arbuscular mycorrhizal Camptotheca acuminata seedlings. Notes see Fig. 1.

Fig. 4 Effect of inoculation time on camptothecin allocation to organs in arbuscular mycorrhizal Camptotheca acuminata seedlings. Notes see Fig. 1.

References 25

[1]	Abu-Zeyad R, Khan AG, Khoo C (1999). Occurrence of arbuscular mycorrhiza in Castanospermum australe A. Cunn. & C. Fraser and effects on growth and production of castanospermine. Mycorrhiza, 9, 111-117.
[2]	Araim G, Saleem A, Arnason JT, Charest C (2009). Root colonization by an arbuscular mycorrhizal (AM) fungus increases growth and secondary metabolism of purple coneflower, Echinacea purpurea (L.) Moench. Journal of Agricultural and Food Chemistry, 57, 2255-2258. DOI URL PMID
[3]	Dai SJ (戴绍军), Wang Y (王洋), Yan XF (阎秀峰), Ma MF (马梅芳) (2004). Effects of color films on growth and camptothecin content in the leaves of Camptotheca acuminata seedlings. Acta Ecologica Sinica (生态学报), 24, 869-875. (in Chinese with English abstract)
[4]	Fan JH (范继红), Yang GT (杨国亭), Mu LQ (穆立蔷), Zhou JH (周加海) (2006). Effect of AMF on the content of berberine, jatrorrhizine and palmatine of Phellodendron amurense seedlings. Protection Forest Science and Technology (防护林科技), (5), 24-26. (in Chinese with English abstract)
[5]	Fester T, Schmidt D, Lohse S, Walter MH, Giuliano G, Bramley PM, Fraser PD, Hause B, Strack D (2002). Stimulation of carotenoid metabolism in arbuscular mycorrhizal roots. Planta, 216, 148-154. URL PMID
[6]	Fester T, Wray V, Nimtz M, Strack D (2005). Is stimulation of carotenoid biosynthesis in arbuscular mycorrhizal roots a general phenomenon? Phytochemistry, 66, 1781-1786. DOI URL PMID
[7]	Gai JP (盖京苹), Feng G (冯固), Li XL (李晓林) (2004). The effect of AM fungi on the growth of sweet potato. Chinese Journal of Eco-Agriculture (中国生态农业学报), 12(1), 111-113. (in Chinese with English abstract)
[8]	Huang TF (黄天芳) (2003). The important roles of secondary metabolites in plant survival. Journal of Biology (生物学杂志), 20(5), 60-61.
[9]	Liu WZ (刘文哲), Zhang AX (张爱新) (2003). Effect of camptothecin on growth of fungal endophytes from Camptotheca acuminata. Acta Botanica Boreali-Occi- dentalia Sinica (西北植物学报), 23, 1275-1278.
[10]	Liu Z, Carpenter SB, Bourgeois WJ, Yu Y, Constantin RJ, Falcon MJ, Adams JC (1998). Variations in the secondary metabolite camptothecin in relation to tissue age and season in Camptotheca acuminata. Tree Physiology, 18, 265-270. URL PMID
[11]	Liu ZJ, Carpenter SB, Constantin RJ (1997). Camptothecin production in Camptotheca acuminata seedlings in response to shading and flooding. Canadian Journal of Botany, 75, 368-373. DOI URL
[12]	Phillips JM, Hayman DS (1970). Improved procedures for clearing and attaining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of the British Mycological Society, 55, 158-161. DOI URL
[13]	Rojas-Andrade R, Cerda-Garcia-Rojas CM, Frias-Hernandez JT, Dendooven L, Olalde-Portugal V, Ramos-Valdivia AC (2003). Changes in the concentration of trigonelline in a semi-arid leguminous plant (Prosopis laevigata) induced by an arbuscular mycorrhizal fungus during the presymbiotic phase. Mycorrhiza, 13, 49-52. URL PMID
[14]	Schliemann W, Kolbe B, Schmidt J, Nimtz M, Wray N (2008). Accumulation of apocarotenoids in mycorrhizal roots of leek (Allium porrum). Phytochemistry, 69, 1680-1688. DOI URL PMID
[15]	Schliemann W, Schmidt J, Nimtz M, Wray V, Fester T, Strack D (2006). Accumulation of apocarotenoids in mycorrhizal roots of Ornithogalum umbellatum. Phytochemistry, 67, 1196-1205. URL PMID
[16]	Vierheilig H, Gagnon H, Strack D, Maier W (2000). Accumulation of cyclohexenone derivatives in barley, wheat and maize roots in response to inoculation with different arbuscular mycorrhizal fungi. Mycorrhiza, 9, 291-293. DOI URL
[17]	Walter MH, Fester T, Strack D (2000). Arbuscular mycorrhizal fungi induce the non-mevalonate methylerythritol phosphate pathway of isoprenoid biosynthesis correlated with accumulation of the ‘yellow pigment’ and other apocarotenoids. The Plant Journal, 21, 571-578. URL PMID
[18]	Wang Y (王洋), Dai SJ (戴绍军), Yan XF (阎秀峰) (2004). Effects of light intensity on secondary metabolite camptothecin production in leaves of Camptotheca acuminata seedlings. Acta Ecologica Sinica (生态学报), 24, 1118-1122. (in Chinese with English abstract)
[19]	Wei GT (魏改堂), Wang HG (汪洪钢) (1989). Effects of VA mycorrhizal fungi on growth, nutrient uptake and effective compounds in Chinese medicinal herb Datura stramonium L. Scientia Agricultura Sinica (中国农业科学), 22, 56-61. (in Chinese with English abstract)
[20]	Yan XF (阎秀峰), Wang Y (王洋), Yu T (于涛) (2002). Determination of camptothecin in leaves of Camptotheca acuminata Decne by HPLC. Journal of Instrumental Analysis (分析测试学报), 21, 15-18. (in Chinese with English abstract)
[21]	Yan XF, Wang Y, Yu T, Zhang YH, Dai SJ (2003). Variation in camptothecin content in Camptotheca acuminata leaves. Botanical Bulletin of Academia Sinica, 44, 99-105.
[22]	Zhao X (赵昕), Wang BW (王博文), Yan XF (阎秀峰) (2006). Effect of arbuscular mycorrhiza on camptothecin content in Camptotheca acuminata seedlings. Acta Ecologica Sinica (生态学报), 26, 1057-1062. (in Chinese with English abstract)
[23]	Zhao X (赵昕), Yan XF (阎秀峰) (2006). Effect of arbuscular mycorrhiza fungi on the growth and absorption of nitrogen and phosphorus in Camptotheca acuminata seedlings. Journal of Plant Ecology (Chinese Version) (植物生态学报), 30, 947-953. (in Chinese with English abstract)
[24]	Zhao X, Wang Y, Yan XF (2007). Effect of arbuscular mycorrhiza fungi and phosphorus on camptothecin content in Camptotheca acuminata seedlings. Allelopathy Journal, 20, 51-60.
[25]	Zhou JH (周加海), Fan JH (范继红) (2007). Effects of AM fungi on the berberine content in Phellodendron chinense seedlings. Northern Horticulture (北方园艺), 12, 25-27. (in Chinese with English abstract)