RAPD ANALYSIS ON GENETIC DIVERSITY OF CARYA DABIESHANENSIS POPULATIONS

doi:10.17521/cjpe.2006.0070

Abstract

Abstract:

The genetic diversity and genetic variation within and among three natural Carya dabieshanensis populations were studied using RAPD analysis. The result showed that 238 loci were detected by using 20 random primers (10 bp) of which 162 loci were polymorphic (68.1%). Species genetic diversity indicated by Shannon's index was 0.476 1, 58.18% of which was due to within population genetic diversity and 41.82% among population variation. Species gene diversity indicated by Nei's index was 0.314 5, gene diversity within populations (H_S) was 0.186 5, gene diversity among populations (H_ST) was 0.128 0 and the coefficient of gene differentiation (G_ST) among the populations was 0.406 7, 59.33% of which was within populations and 40.67% was among populations. The gene diversity estimated by Nei's index was consistent with that estimated by Shannon's index. These results suggest that there is rich genetic within population variation, which offers excellent prospects for seeding selection. The estimate of gene flow from G_ST (N_m) was 0.730 6, indicating that genetic recombination between populations is quite small, which probably is related to environmental adaptations of C. debieshanensis and population isolation in their high mountain habitat.

Key words: Carya dabieshanensis, Population, RAPD analysis, Genetic diversity

WANG Zheng-Jia, HUANG You-Jun, GUO Chuan-You, HUANG Jian-Qin, WANG Hua-Fang. RAPD ANALYSIS ON GENETIC DIVERSITY OF CARYA DABIESHANENSIS POPULATIONS[J]. Chin J Plant Ecol, 2006, 30(3): 534-538.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2006.0070

https://www.plant-ecology.com/EN/Y2006/V30/I3/534

Figures/Tables 5

References 27

[1]	Alpert P, Lumaret R, Giusto FD (1993). Population structure inferred from allozyme analysis in the clonal herb Fragariao biloensis (Rosaceae) . American Journal of Botany, 80,1002-1006.
[2]	Gao HD (高捍东) (2001). Genetic analysis of cultivers of chestnut ( Castauea mollisima) by technique of RAPD . Journal of Jiangsu Forestry Science & Technology (江苏林业科技), 28,1-3. (in Chinese with English abstract)
[3]	Ge S (葛颂), Hong DY (洪德元) (1994). Genetic diversity and its detection.In: Qian YQ(钱迎倩),Ma KP(马克平) eds. Principles and Methodologies of Biodiversity Studies (生物多样性研究的原理与方法).China Science and Technology Press, Beijing,123-140. (in Chinese)
[4]	Ge S (葛颂), Hong DY (洪德元) (1999). Studies of morphological and allozyme variation of the endangered Adenophom lobophylla and its widespread congener A.potaninii. potaninii. Acta Genetica Sinica (遗传学报), 26,410-417. (in Chinese with English abstract)
[5]	Ge S (葛颂), Wang HQ (王海群), Zhang CM (张灿明), Hong DY (洪德元) (1997). Genetic diversity and population differentiation of Cathaya argyrophlla in Bamian Mountain . Acta Botanica Sinica (植物学报), 39,266-271. (in Chinese with English abstract)
[6]	Guo CY (郭传友), Huang JQ (黄坚钦), Wang ZJ (王正加), Fang YM (方炎明) (2004). Preliminary study on the Carya dabieshanensis community of Tiantangzhai Mountains in Anhui Province . Guihaia (广西植物), 24,97-101. (in Chinese with English abstract)
[7]	Huang JX (黄久香), Zhuang XY (庄雪影) (2002a). Comparison of genetic diversity of Tsoongiodendron odorum in southern China by RAPD marker . Journal of South China Agricultural University (华南农业大学学报), 23,54-57. (in Chinese with English abstract)
[8]	Huang JX (黄久香), Zhuang XY (庄雪影) (2002b). Genetic diversity of the population of Tsoongiodendron odorum . Acta Phytoecologica Sinica (植物生态学报), 26,413-419. (in Chinese with English abstract)
[9]	Huang QQ (黄启强), Wang LH (王莲辉), Nobuhiro T,Kiihachiro O(1995). The genetic variation of isozyme in natural population of mass pine. Acta Genetica Sinica (遗传学报), 22,142-151. (in Chinese with English abstract)
[10]	Kiang YT, Chiang YC (1990). Comparing differentiation of wild bean ( Glycinesoja siebandzucc) populations on isozymes and quantitative traits . Botanical Bulletin Academic Sinica, 131,129-142.
[11]	Li J (李军), Tao Y (陶芸), Zheng SZ (郑师章), Zhou JL (周纪纶) (1995). Isozymatic diffrenetiation in local population of Glycine soja Sieb. & Zucc . Acta Botanica Sinica (植物学报), 37,669-676. (in Chinese with English abstract)
[12]	Li QM (李巧明), Xu ZF (许再富), He TH (何田华) (2002). A preliminary study on conservation genetic of endangered Vatica guangxiensis (Dipterocarpaceace) . Acta Botanica Sinica (植物学报), 44,246-249. (in Chinese with English abstract)
[13]	Lin XC (林新春), Yu ZX (俞志雄), Qiu LH (裘利洪), Xiao GM (肖国民), Liu L (刘力) (2003). Studies on genetic diversity of endangered Sinomanglietia glauca (Magnoliaceae) . Acta Agriculturae Universitatis JingXiensis (江西农业大学学报), 25,805-810. (in Chinese with English abstract)
[14]	Liu MC (刘茂春), Li ZJ (黎章矩) (1984). A new species of Carya from China . Journal of Zhejiang College of Forestry (浙江林学院学报), 1,41-43. (in Chinese with English abstract)
[15]	Loveless MD, Hamrick JLH (1984). Ecological determinants of genetic structure in plant populations. Annual Review Ecology System, 15,65-95.
[16]	Luo GZ (罗光佐), Shi JS (施季森), Yin TM (尹佟明), Huang MR (黄敏仁), Wang MX (王明庥) (2001). Comparison of genetic diversity between Liriodendron tulipifera Linn. and L. chinense (Hemsl.) Sarg. by means of RAPD markers . Journal of Plant Resources and Environment (植物资源与环境), 9,9-13. (in Chinese with English abstract)
[17]	Pich C, Schubert I (1993). Miniperp method for isolation of DNA form plants with a high content of polyphenolics. Nucleic Acids Research, 21,3328-3332. URL PMID
[18]	Song CW (宋丛文), Bao MZ (包满珠) (2004). Study on genetic diversity of RAPD mark for natural Davidia involucrate population . Scientia Silvae Sinicae (林业科学), 40,75-79. (in Chinese with English abstract)
[19]	Su XH (苏晓华), Zhang QW (张绮纹), Zheng XW (郑先武), Zhang XH (张香华), Gui F (归复) (1997). Genetic structure in Populus ussuriensis Kon. confirmed by RAPD marker . Scientia Silvae Sinicae (林业科学), 33,504-512. (in Chinese with English abstract)
[20]	Wang ZJ (王正加), Huang JQ (黄坚钦), Guo CY (郭传友), Liang JF (梁景峰) (2005). Genomic DNA extraction and RAPD primer screening of Carya cathayensis . Journal of Anhui Agricultural University (安徽农业大学学报), 32,72-76. (in Chinese with English abstract)
[21]	Wang ZJ (王正加), Huang JQ (黄坚钦), Guo CY (郭传友), Yang P (杨萍) (2003). The optimal reaction system of RAPD in Carya cathayensis . Journal of Zhejiang College of Forestry (浙江林学院学报), 20,429-433. (in Chinese with English abstract)
[22]	Wang XQ (汪小全), Zou YP (邹喻萍), Zhang DM (张大明) (1996). Analysis of genetic diversity in Cathaya argyrophylla using Random Amplified Polymorphic DNA (RAPD) Markers . Science in China(Series C) (中国科学C辑), 26,436-441. (in Chinese with English abstract)
[23]	Wright S (1931). Evolution populations. Genetics, 16,97-159. URL PMID
[24]	Xia M (夏铭), Zhou XF (周晓峰), Zhao SD (赵士洞) (2001). RAPD analysis on genetic diversity of natural population of Quercus mongolica. Scientia Silvae Sinicae (林业科学), 37,126-133. (in Chinese with English abstract)
[25]	Yet FC, Chang DKX, Yang RC (1995). RAPD variation within and among natural population of trembling aspen from Alberta. The Journal of Heredity, 8,454-459.
[26]	Yun R (恽锐), Zhong M (钟敏), Wang HX (王洪新), Wei W (魏伟), Hu ZA (胡志昂), Qian YQ (钱迎倩) (1998). Study on DNA diversity of Liaodong oak population at Donglin mountain region,Beijing . Acta Botanica Sinica (植物学报), 40,169-175. (in Chinese with English abstract)
[27]	Zhu XF (祝学范), Zhu XL (祝学林) (2001). Investigation on resource and development prospect of Carya cathayensis in Jingzhai . Journal of Anhui Forestry Science and Technology (安徽林业科技), 29,684-685. (in Chinese with English abstract)

种群 Population	地理位置 Geographical location	海拔 Elevation (m)
西园 Xiyuan	31°10' N, 115°37' E	530
仙桃 Xiantao	31°41' N, 115°40' E	750
平田 Pingtian	30°37' N, 114°54' E	640

种群 Population	地理位置 Geographical location	海拔 Elevation (m)
西园 Xiyuan	31°10' N, 115°37' E	530
仙桃 Xiantao	31°41' N, 115°40' E	750
平田 Pingtian	30°37' N, 114°54' E	640

种群 Population	样本数 Number of samples	位点数 Number of loci	多态位点数 Number of polymorphic loci	多态位点百分率 Percentage of polymorphic loci
西园 Xiyuan	30	238	129	54.0
平田 Pingtian	30	238	131	55.2
仙桃 Xiantao	30	238	127	53.2
总计Total	240	238	162	68.1

种群 Population	样本数 Number of samples	位点数 Number of loci	多态位点数 Number of polymorphic loci	多态位点百分率 Percentage of polymorphic loci
西园 Xiyuan	30	238	129	54.0
平田 Pingtian	30	238	131	55.2
仙桃 Xiantao	30	238	127	53.2
总计Total	240	238	162	68.1

	西园 Xiyuan	平田 Pingtian	仙桃 Xiantao	种的基因多样性 Species genetic diversity	种群内基因多样性 Within-population genetic diversity (H_s)	种群间基因多样性 Inter-population genetic diversity (H_ST)	种群间基因分化系数 Coefficient of gene differentiation (G_ST)
Shonnon多样性指数 Shonnon Index (I)	0.279 8	0.280 1	0.265 1	0.476 1	0.275 0	0.201 1	0.418 2
Nei指数 Nei index	0.189 0	0.191 6	0.178 9	0.314 5	0.186 5	0.128 0	0.406 3