RESPONSE OF GRACILARIA LEMANEIFORMIS TO NITROGEN AND PHOSPHORUS EUTROPHIC SEAWATER

doi:10.17521/cjpe.2007.0063

Abstract

Abstract:

Aims Gracilaria lemaneiformis is a commercially important marine red alga from which agar is extracted for use in food chemistry and in production of tissue culture media. It has been transplanted from Qingdao, Shandong Province to Nan'ao Island, Guangdong Province and other places in South China. Understanding the relationship between its photosynthesis and environmental factors has theoretical and practical importance for growing the macroalga more rationally, understanding its biological functions and increasing its utilization.
Methods We cultured G. lemaneiformis seaweeds in Qing'ao Bay of Nan'ao Island, South Sea, China. The silky samples (immersed in seawater in plastic casks) were taken into a laboratory and provided oxygen by air pumps. We studied their physiological responses to six days of growth in seawater eutrophicated to different degrees by nitrogen and phosphorus alone and in combination.
Important findings Contents of phycobiliprotein and chlorophyll a and total antioxidative ability were decreased significantly in mild phosphorus eutrophic seawater (P 0.2 mg·L^-1) and in severe nigrogen+phosphorus eutrophic seawater (P 10 mg·L^-1, N 55 mg·L^-1). Chlorophyll fluorescence kinetic parameters (F_v/F_m, F_v/F_o, q_P, q_N, Φ_PSⅡ, q_o) also showed the harmful effect of such treatments to PSⅡ function. However, no obvious changes in these parameters were found with mild nitrogen (N 0.9 mg·L^-1) and mild nitrogen+phosphorus (P 0.2 mg·L^-1, N 0.9 mg·L^-1) eutrophic seawater. Gracilaria lemaneiformis did not accumulate more nitrogen or phosphorus in vivo in mild eutrophic seawater than in the control seawater. Because G. lemaneiformis is tolerant to mild N and mild N+P pollution, this species may be used to purify lightly eutrophicated seawater.

Key words: Gracilaria lemaneiformis, eutrophication, nitrogen, phosphorus, chlorophyll fluorescence parameters, phycobiliprotein

PENG Chang-Lian, WEN Xue, LIN Zhi-Fang, ZHOU Hou-Cheng, CHEN Shao-Wei, LIN Gui-Zhu. RESPONSE OF GRACILARIA LEMANEIFORMIS TO NITROGEN AND PHOSPHORUS EUTROPHIC SEAWATER[J]. Chin J Plant Ecol, 2007, 31(3): 505-512.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2007.0063

https://www.plant-ecology.com/EN/Y2007/V31/I3/505

Figures/Tables 4

Fig.1 Changes in phycobiliproteins, contents of chlorophyll a and carotenoid in Gracilaria lemaneiformis after 6 days treatment in seawater with different concentrations of nitrogen and phosphorus CK (control), original seawater; L-P, less polluted seawater by 0.2 mg·L-1 of phosphorous (NaH2PO4); L-N, less polluted seawater by 0.9 mg·L-1 nitrogen (NH4NO3); L-NP, less polluted by nitrogen (0.9 mg·L-1)and phosphorus (0.2 mg·L-1) combination; H-NP, serious polluted seawater by 10 mg·L-1 nitrogen and 55 mg·L-1 phosphorus. The numbers sharing the same letters are not significantly different (p=0.05), and these with other different letters are significantly different (p<0.05)

Fig.2 Changes in chlorophyll a fluorescence parameters in Gracilaria lemaneiformis after 6 days treatment with different concentrations of nitrogen and phosphorus Fv/Fo:PSⅡ潜在活性 PSⅡ potential activity Fv/Fm: PSⅡ原初光化学效率 Maximum quantum yield of photosynthesis qo: 叶绿素荧光最小值的相对变化 Relative change of minimum chlorophll fluorescence qN: 非光化学猝灭系数 Non-photochemical quenching qP: 光化学猝灭系数 Photochemical quenching ΦPSⅡ: 光合作用的真实量子效率 Actual quantumn yield of photosynthesis CK、L-P、L-N、L-NP、H-NP: 见图1 See Fig. 1

Fig.3 Changes in DPPH· scavenge capacity in Gracilaria lemaneiformis treated with different concentrations of nitrogen and phosphorous CK、L-P、L-N、L-NP、H-NP:See Fig. 1

Fig.4 Changes of nitrogen or phosphorus content in Gracilaria lemaneiformis after treating with different concentrations of nitrogen and phosphorus CK、L-P、L-N、L-NP、H-NP:See Fig. 1

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