Effects of arbuscular mycorrhizal fungi on calorific value and contents of carbon and ash in Robinia pseudoacacia

doi:10.3724/SP.J.1258.2013.00106

Abstract

Abstract:

Aims It is still unknown whether arbuscular mycorrhizal (AM) fungi could increase calorific value and carbon content in plants. Our objectives in this study were to (1) determine the effects of two AM fungi, Glomus intraradices and G. versiforme, on biomass, calorific value, carbon content, and ash content in black locust (Robinia pseudoacacia) seedlings; and (2) assess the effectiveness of G. intraradices and G. versiforme in affecting biomass and energy accumulation in black locust.
Methods Three treatments were performed: black locust seedlings, including inoculations of seedlings with G. intraradices, G. versiforme, respectively, and no inoculum as control. Seedlings were grown in a greenhouse for 14 months following treatments, and then their biomass, gross calorific value, carbon content, and ash content of the roots, stems, and leaves were measured. Ash-free calorific value, energy accumulation, and carbon accumulation were calculated.
Important findings We found that inoculations with the two AM fungi increased the biomass, calorific value, and carbon content in black locust seedlings. The accumulations of biomass, energy, and carbon were 89.61%, 102.20%, and 93.30% greater in black locust seedlings inoculated with G. intraradices, and 91.34%, 94.19% and 77.21% greater in those inoculated with G. versiforme, respectively, than the controls. Both calorific value and carbon content were the highest in seedlings inoculated with G. intraradices; and the gross calorific value of roots, stems and leaves were 7.72%, 8.94%, 8.41% higher, respectively, in seedlings inoculated with G. intraradices than the controls. Glomus intraradices was found to be more effective than G. versiforme in enhancing calorific value and accumulation of energy and carbon.

Key words: arbuscular mycorrhizal fungi, ash content, black locust, calorific value, carbon content

ZHU Xiao-Qin,WANG Chun-Yan,SHENG Min,CHEN Hui,TANG Ming. Effects of arbuscular mycorrhizal fungi on calorific value and contents of carbon and ash in Robinia pseudoacacia[J]. Chin J Plant Ecol, 2013, 37(11): 1028-1034.

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https://www.plant-ecology.com/EN/Y2013/V37/I11/1028

Figures/Tables 4

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	植物器官 Plant organ	根内球囊霉 Glomus intraradices	地表球囊霉 Glomus versiforme	对照 Control
菌根侵染率 Rate of AM colonization (%)		78 ± 3^a	61 ± 4^b	0^c
苗高 Seedling height (cm)		34.3 ± 3.2^b	38.6 ± 3.7^a	18.2 ± 1.2^c
地茎 Basal diameter (mm)		6.7 ± 0.3^a	7.3 ± 0.4^a	5.5 ± 0.8^b
根冠比 Root/shoot ratio		0.73 ± 0.19^a	0.81 ± 0.12^a	0.58 ± 0.25^a
干重 Dry weight (g·plant^-1)	根 Root	8.30 ± 1.42^a	8.87 ± 0.86^a	3.66 ± 0.94^b
	茎 Stem	4.23 ± 0.49^a	3.95 ± 0.35^a	1.78 ± 0.64^b
	叶 Leaf	7.18 ± 0.57^a	7.07 ± 0.47^a	4.95 ± 1.03^b
	整株 Whole plant	19.72 ± 0.50^a	19.90 ± 0.51^a	10.40 ± 0.76^b

	植物器官 Plant organ	根内球囊霉 Glomus intraradices	地表球囊霉 Glomus versiforme	对照 Control
菌根侵染率 Rate of AM colonization (%)		78 ± 3^a	61 ± 4^b	0^c
苗高 Seedling height (cm)		34.3 ± 3.2^b	38.6 ± 3.7^a	18.2 ± 1.2^c
地茎 Basal diameter (mm)		6.7 ± 0.3^a	7.3 ± 0.4^a	5.5 ± 0.8^b
根冠比 Root/shoot ratio		0.73 ± 0.19^a	0.81 ± 0.12^a	0.58 ± 0.25^a
干重 Dry weight (g·plant^-1)	根 Root	8.30 ± 1.42^a	8.87 ± 0.86^a	3.66 ± 0.94^b
	茎 Stem	4.23 ± 0.49^a	3.95 ± 0.35^a	1.78 ± 0.64^b
	叶 Leaf	7.18 ± 0.57^a	7.07 ± 0.47^a	4.95 ± 1.03^b
	整株 Whole plant	19.72 ± 0.50^a	19.90 ± 0.51^a	10.40 ± 0.76^b

	植物器官 Plant organ	根内球囊霉 Glomus intraradices	地表球囊霉 Glomus versiforme	对照 Control
干重热值 Gross calorific value (kJ·g^-1)	根 Root	14.50 ± 0.24^a	13.33 ± 0.29^b	13.46 ± 0.24^b
	茎 Stem	16.80 ± 0.13^a	16.32 ± 0.24^b	15.42 ± 0.12^c
	叶 Leaf	17.65 ± 0.16^a	17.37 ± 0.84^a	16.28 ± 0.76^b
去灰分热值 Ash-free calorific value (kJ·g^-1)	根 Root	16.52 ± 0.40^a	15.33 ± 0.47^b	15.27 ± 0.51^b
	茎 Stem	18.46 ± 0.21^a	18.05 ± 0.29^a	17.03 ± 0.24^b
	叶 Leaf	20.26 ± 0.23^a	19.82 ± 0.07^a	18.74 ± 0.30^b

	植物器官 Plant organ	根内球囊霉 Glomus intraradices	地表球囊霉 Glomus versiforme	对照 Control
干重热值 Gross calorific value (kJ·g^-1)	根 Root	14.50 ± 0.24^a	13.33 ± 0.29^b	13.46 ± 0.24^b
	茎 Stem	16.80 ± 0.13^a	16.32 ± 0.24^b	15.42 ± 0.12^c
	叶 Leaf	17.65 ± 0.16^a	17.37 ± 0.84^a	16.28 ± 0.76^b
去灰分热值 Ash-free calorific value (kJ·g^-1)	根 Root	16.52 ± 0.40^a	15.33 ± 0.47^b	15.27 ± 0.51^b
	茎 Stem	18.46 ± 0.21^a	18.05 ± 0.29^a	17.03 ± 0.24^b
	叶 Leaf	20.26 ± 0.23^a	19.82 ± 0.07^a	18.74 ± 0.30^b

	植物器官 Plant organ	根内球囊霉 Glomus intraradices	地表球囊霉 Glomus versiforme	对照 Control
含碳量 Carbon content (%)	根 Root	29.63 ± 1.34^a	23.45 ± 1.62^c	26.04 ± 0.23^b
	茎 Stem	42.09 ± 1.86^a	39.16 ± 0.64^a	40.94 ± 1.59^a
	叶 Leaf	47.81 ± 1.51^a	44.62 ± 5.77^a	42.76 ± 3.98^a
灰分含量 Ash content (%)	根 Root	12.22 ± 0.74^a	13.06 ± 0.85^a	11.81 ± 1.38^a
	茎 Stem	8.96 ± 3.30^b	9.60 ± 0.56^a	9.47 ± 0.66^a
	叶 Leaf	12.90 ± 0.31^a	12.36 ± 0.45^a	13.11 ± 0.87^a