EFFECTS OF ENHANCED UV-B RADIATION ON WATER USE EFFICIENCY, STOMATAL CONDUCTANCE, LEAF NITROGEN CONTENT AND MORPHOLOGICAL CHARACTERISTICS OF SPIRAEA PUBESCENS IN A WARM-TEMPERATE DECIDUOUS BROAD-LEAF FOREST

doi:10.17521/cjpe.2006.0007

Abstract

Abstract:

Spiraea pubescens, a common shrub in the warm-temperate deciduous forest zone, is distributed in the Dongling Mountain area of Beijing, was exposed to ambient and enhanced ultraviolet-B (UV-B, 280-320 nm) radiation by artificially supplying a daily dose of 9.4 kJ·m^-2 for three growing seasons, a level that simulated a 17% depletion in stratospheric ozone. The objective of this study was to explore the effects of long-term UV-B enhancement on stomatal conductance, leaf tissue δ¹³C, leaf water content, and leaf area. Particular attention was paid to the effects of UV-B radiation on water use efficiency (WUE) and leaf total nitrogen content. Enhanced UV-B radiation significantly reduced leaf area (50.1%) but increased leaf total nitrogen content (102%). These changes were associated with a decrease in stomatal conductance (16.1%) and intercellular CO₂ concentration/air CO₂ concentration (C_i/C_a) (4.0%), and an increase in leaf tissue δ¹³C (20.5‰), leaf water content (3.1%), specific leaf weight (SLW) (5.2%), and WUE (4.1%). The effects of UV-B on the plant were greatly affected by the water content of the deep soil (30-40 cm). During the dry season, differences in the stomatal conductance, δ¹³C, and WUE between the control and UV-B treated shrubs were very small, whereas differences became much greater when soil water stress disappeared. Furthermore, the effects of UV-B became much less significant as the treatment period progressed over the three growing seasons. Correlation analysis showed that enhanced UV-B radiation decreased the strength of the correlation between soil water content and leaf water content, δ¹³C, C_i/C_a, stomatal conductance, with the exception of WUE that had a significant correlation coefficient with soil water content. These results suggest that WUE would become more sensitive to soil water variation due to UV-B radiation. Based on this experiment, it was found that enhanced UV-B radiation had much more significant effects on morphological traits and growth of S. pubescens than hydro-physiological characteristics.

Key words: Warm-temperate deciduous broad-leaved forest, Spiraea pubescens, UV-B radiation, δ¹³C, Water use efficiency, Nutrition content

CHEN Lan, ZHANG Shou-Ren. EFFECTS OF ENHANCED UV-B RADIATION ON WATER USE EFFICIENCY, STOMATAL CONDUCTANCE, LEAF NITROGEN CONTENT AND MORPHOLOGICAL CHARACTERISTICS OF SPIRAEA PUBESCENS IN A WARM-TEMPERATE DECIDUOUS BROAD-LEAF FOREST[J]. Chin J Plant Ecol, 2006, 30(1): 47-56.

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Figures/Tables 12

References 41

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时间 Time		2002				2003				2004
时间 Time		6月 Jun.	7月 Jul.	8月 Aug.	9月 Sept.	6月 Jun.	7月 Jul.	8月 Aug.	9月 Sept.	6月 Jun.	7月 Jul.	8月 Aug.	9月 Sept.
月降雨量 Monthly precipitation (MP) (mm)		118.1	125.0	60.4	90.5	78.6	87.2	61.3	82.7	78.1	227.2	95.8	57.4
大气相对湿度 Relative humidity (RH) (%)		79	84	89	85	78	86	86	86	77	87	90	82
月平均温度 Monthly mean temperature (MT) (℃)		16.1	19.0	17.6	11.8	15.5	17.6	16.5	13.0	16.4	17.6	15.9	12.1
叶片空气水气压亏缺 Leaf-to-air	UV-B	-	-	-	-	-	-	-	-	8.65	7.24	6.06	6.06
vapour pressure deficit (VPD) (hPa)	对照 Control	-	-	-	-	-	-	-	-	8.67	7.12	6.07	6.26
空气CO₂浓度 Air CO₂ concentration (C_a) (μmol·mol^-1)			-				-				370.4
空气CO₂的碳同位素比率 Carbon isotope ratio (δ¹³C ) (‰)			-				-				-10.34

时间 Time		2002				2003				2004
时间 Time		6月 Jun.	7月 Jul.	8月 Aug.	9月 Sept.	6月 Jun.	7月 Jul.	8月 Aug.	9月 Sept.	6月 Jun.	7月 Jul.	8月 Aug.	9月 Sept.
月降雨量 Monthly precipitation (MP) (mm)		118.1	125.0	60.4	90.5	78.6	87.2	61.3	82.7	78.1	227.2	95.8	57.4
大气相对湿度 Relative humidity (RH) (%)		79	84	89	85	78	86	86	86	77	87	90	82
月平均温度 Monthly mean temperature (MT) (℃)		16.1	19.0	17.6	11.8	15.5	17.6	16.5	13.0	16.4	17.6	15.9	12.1
叶片空气水气压亏缺 Leaf-to-air	UV-B	-	-	-	-	-	-	-	-	8.65	7.24	6.06	6.06
vapour pressure deficit (VPD) (hPa)	对照 Control	-	-	-	-	-	-	-	-	8.67	7.12	6.07	6.26
空气CO₂浓度 Air CO₂ concentration (C_a) (μmol·mol^-1)			-				-				370.4
空气CO₂的碳同位素比率 Carbon isotope ratio (δ¹³C ) (‰)			-				-				-10.34

变异来源 Variation	df	气孔导度 Stomatal conductance		δ¹³C值 Carbon isotope ratio		水分利用效率 WUE		叶含水量 Leaf water content		胞间CO₂浓度与大气CO₂ 浓度之比 C_i/C_a		叶面积 Leaf area		比叶重 SLW		全氮含量 Total N content
变异来源 Variation	df	F	p		F	p		F	p		F	p		F	p		F	p	F	p	F	p
主效应 Main effects
UV-B	1	2.105	ns	0.699	ns	1.165	ns	4.034	ns	0.936	ns	23.601	***	3.283	ns	15.766	**
时间 Time	3	5.81	*	4.073	*	24.457	***	3.58	*	4.014	*	0.291	ns	2.726	ns	1.257	ns
二元交互 Two-factor interaction effects
UV-B×时间 UV-B×Time	3	0.658	ns	1.179	ns	1.164	ns	0.228	ns	0.927	ns	0.096	ns	0.296	ns	1.764	ns

变异来源 Variation	df	气孔导度 Stomatal conductance		δ¹³C值 Carbon isotope ratio		水分利用效率 WUE		叶含水量 Leaf water content		胞间CO₂浓度与大气CO₂ 浓度之比 C_i/C_a		叶面积 Leaf area		比叶重 SLW		全氮含量 Total N content
变异来源 Variation	df	F	p		F	p		F	p		F	p		F	p		F	p	F	p	F	p
主效应 Main effects
UV-B	1	2.105	ns	0.699	ns	1.165	ns	4.034	ns	0.936	ns	23.601	***	3.283	ns	15.766	**
时间 Time	3	5.81	*	4.073	*	24.457	***	3.58	*	4.014	*	0.291	ns	2.726	ns	1.257	ns
二元交互 Two-factor interaction effects
UV-B×时间 UV-B×Time	3	0.658	ns	1.179	ns	1.164	ns	0.228	ns	0.927	ns	0.096	ns	0.296	ns	1.764	ns

		水分利用效率 WUE	叶含水量 Leaf water content	δ¹³C值 Carbon isotope ratio	胞间CO₂浓度与大气CO₂浓度之比C_i/C_a	气孔导度 Stomatal conductance
UV-B	Pearson correlation	0.623*	-0.467	-0.078	0.073	0.275
	Sig. (2-tailed)	0.03	0.126	0.81	0.822	0.475
	n	12	12	12	12	9
对照	Pearson correlation	0.604	-0.731*	-0.608	0.540	0.711
Control	Sig. (2-tailed)	0.113	0.04	0.11	0.168	0.114
	n	8	8	8	8	6