Leaf carbon, nitrogen and phosphorus stoichiometry at different growth stages in dominant tree species of a monsoon broad-leaved evergreen forest in Pu’er, Yunnan Province, China

doi:10.17521/cjpe.2015.0006

Abstract

Abstract: Aims

Our objective was to explore the effect of growth stage on leaf C:N:P stoichiometry in dominant species of a monsoon broad-leaved evergreen forest in Pu’er, Yunnan Province.

Methods

We collected leaves from six dominant species (i.e. Castanopsis hystrix, C. echidnocarpa, Lithocarpus fenestratus, L. truncatus, Schima wallichii and Anneslea fragrans) at different growth stages from seedlings to mature trees. The leaf N, P and C contents were measured. The effects of species, growth stage and their interaction on leaf C, N and P contents and stoichiometry were analyzed by Repeated-Measure ANOVA in SPSS 19.0.

<i>Important findings</i>

The ranges of leaf N, P and C contents and mass ratios of C:N, N:P and C:P in the six species across different growth stages were 7.90-17.72 mg·g^-1, 0.34-1.39 mg·g^-1, 458.48-516.87 mg·g^-1, 28.04- 65.70, 11.41-63.50 and 355.23-1878.17, respectively. The coefficient of variation (CV) for N:P mass ratio ranged between 19.19%-91.65%, with an average of 36.46%; CV for C:P varied between 15.99%-91.60%, with an average of 34.80%; whilst CV for C varied between 1.61%-5.89%, with an average of 3.12%. Factorial analysis of the variation for each variable, with growth stage and species as independent factors, showed that leaf N and C contents and the mass ratios of leaf C:N, N:P and C:P were mainly determined by growth stage, while leaf P content was mainly determined by an interaction between growth stage and species.

Key words: stoichiometry, dominant species, monsoon broad-leaved evergreen forest, interaction, coefficient of variation

LIU Wan-De,SU Jian-Rong,LI Shuai-Feng,LANG Xue-Dong,ZHANG Zhi-Jun,HUANG Xiao-Bo. Leaf carbon, nitrogen and phosphorus stoichiometry at different growth stages in dominant tree species of a monsoon broad-leaved evergreen forest in Pu’er, Yunnan Province, China[J]. Chin J Plan Ecolo, 2015, 39(1): 52-62.

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URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2015.0006

https://www.plant-ecology.com/EN/Y2015/V39/I1/52

Figures/Tables 6

References 40

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物种 Species	科 Family	属 Genus
红锥 Castanopsis hystrix	壳斗科 Fagaceae	锥属 Castanopsis
短刺锥 C. echidnocarpa	壳斗科 Fagaceae	锥属 Castanopsis
泥柯 Lithocarpus fenestratus	壳斗科 Fagaceae	柯属 Lithocarpus
截果柯 L. truncatus	壳斗科 Fagaceae	柯属 Lithocarpus
西南木荷 Schima wallichii	山茶科 Theaceae	木荷属 Schima
茶梨 Anneslea fragrans	山茶科 Theaceae	茶梨属 Anneslea

物种 Species	科 Family	属 Genus
红锥 Castanopsis hystrix	壳斗科 Fagaceae	锥属 Castanopsis
短刺锥 C. echidnocarpa	壳斗科 Fagaceae	锥属 Castanopsis
泥柯 Lithocarpus fenestratus	壳斗科 Fagaceae	柯属 Lithocarpus
截果柯 L. truncatus	壳斗科 Fagaceae	柯属 Lithocarpus
西南木荷 Schima wallichii	山茶科 Theaceae	木荷属 Schima
茶梨 Anneslea fragrans	山茶科 Theaceae	茶梨属 Anneslea

参数 Para- meter	物种 Species	平均值 Mean	变异系数 Coefficient of variation (%)	极差 Range
N	茶梨 Anneslea fragrans	8.78	15.72	5.40
	红锥 Castanopsis hystrix	12.73	11.23	4.54
	短刺锥 C. echidnocarpa	14.20	13.66	8.65
	西南木荷 Schima wallichii	12.75	27.61	14.17
	泥柯 Lithocarpus fenestratus	17.01	7.47	5.72
	截果柯 L. truncatus	14.55	15.60	7.19
	合计 Total	13.24	24.24	17.85
P	茶梨 Anneslea fragrans	0.46	17.39	0.35
	红锥 Castanopsis hystrix	0.40	17.50	0.22
	短刺锥 C. echidnocarpa	0.71	66.20	1.41
	西南木荷 Schima wallichii	0.41	19.51	0.28
	泥柯 Lithocarpus fenestratus	1.02	63.73	1.71
	截果柯 L. truncatus	0.53	18.87	0.33
	合计 Total	0.59	40.68	1.71
C	茶梨 Anneslea fragrans	508.62	3.00	53.34
	红锥 Castanopsis hystrix	464.75	1.61	20.73
	短刺锥 C. echidnocarpa	480.35	1.97	41.36
	西南木荷 Schima wallichii	471.38	5.89	107.37
	泥柯 Lithocarpus fenestratus	502.91	3.75	57.73
	截果柯 L. truncatus	495.10	2.49	47.95
	合计 Total	487.18	3.12	107.37
C:N	茶梨 Anneslea fragrans	59.49	18.34	43.33
	红锥 Castanopsis hystrix	37.05	13.77	16.21
	短刺锥 C. echidnocarpa	34.36	12.17	17.70
	西南木荷 Schima wallichii	38.84	20.29	32.10
	泥柯 Lithocarpus fenestratus	29.68	6.84	8.99
	截果柯 L. truncatus	34.83	16.48	19.27
	合计 Total	39.30	15.19	66.81
N:P	茶梨 Anneslea fragrans	19.80	24.80	20.96
	红锥 Castanopsis hystrix	32.71	19.20	19.23
	短刺锥 C. echidnocarpa	25.56	38.93	33.09
	西南木荷 Schima wallichii	31.58	23.21	24.57
	泥柯 Lithocarpus fenestratus	31.60	91.65	71.06
	截果柯 L. truncatus	28.46	20.98	21.45
	合计 Total	28.28	37.38	71.77
C:P	茶梨 Anneslea fragrans	1 153.75	22.23	1100.84
	红锥 Castanopsis hystrix	1 195.89	15.99	622.73
	短刺锥 C. echidnocarpa	855.62	35.06	942.17
	西南木荷 Schima wallichii	1 195.06	22.49	911.85
	泥柯 Lithocarpus fenestratus	934.20	91.60	2075.46
	截果柯 L. truncatus	977.11	21.45	729.09
	合计 Total	1 051.94	32.98	2075.46

参数 Para- meter	物种 Species	平均值 Mean	变异系数 Coefficient of variation (%)	极差 Range
N	茶梨 Anneslea fragrans	8.78	15.72	5.40
	红锥 Castanopsis hystrix	12.73	11.23	4.54
	短刺锥 C. echidnocarpa	14.20	13.66	8.65
	西南木荷 Schima wallichii	12.75	27.61	14.17
	泥柯 Lithocarpus fenestratus	17.01	7.47	5.72
	截果柯 L. truncatus	14.55	15.60	7.19
	合计 Total	13.24	24.24	17.85
P	茶梨 Anneslea fragrans	0.46	17.39	0.35
	红锥 Castanopsis hystrix	0.40	17.50	0.22
	短刺锥 C. echidnocarpa	0.71	66.20	1.41
	西南木荷 Schima wallichii	0.41	19.51	0.28
	泥柯 Lithocarpus fenestratus	1.02	63.73	1.71
	截果柯 L. truncatus	0.53	18.87	0.33
	合计 Total	0.59	40.68	1.71
C	茶梨 Anneslea fragrans	508.62	3.00	53.34
	红锥 Castanopsis hystrix	464.75	1.61	20.73
	短刺锥 C. echidnocarpa	480.35	1.97	41.36
	西南木荷 Schima wallichii	471.38	5.89	107.37
	泥柯 Lithocarpus fenestratus	502.91	3.75	57.73
	截果柯 L. truncatus	495.10	2.49	47.95
	合计 Total	487.18	3.12	107.37
C:N	茶梨 Anneslea fragrans	59.49	18.34	43.33
	红锥 Castanopsis hystrix	37.05	13.77	16.21
	短刺锥 C. echidnocarpa	34.36	12.17	17.70
	西南木荷 Schima wallichii	38.84	20.29	32.10
	泥柯 Lithocarpus fenestratus	29.68	6.84	8.99
	截果柯 L. truncatus	34.83	16.48	19.27
	合计 Total	39.30	15.19	66.81
N:P	茶梨 Anneslea fragrans	19.80	24.80	20.96
	红锥 Castanopsis hystrix	32.71	19.20	19.23
	短刺锥 C. echidnocarpa	25.56	38.93	33.09
	西南木荷 Schima wallichii	31.58	23.21	24.57
	泥柯 Lithocarpus fenestratus	31.60	91.65	71.06
	截果柯 L. truncatus	28.46	20.98	21.45
	合计 Total	28.28	37.38	71.77
C:P	茶梨 Anneslea fragrans	1 153.75	22.23	1100.84
	红锥 Castanopsis hystrix	1 195.89	15.99	622.73
	短刺锥 C. echidnocarpa	855.62	35.06	942.17
	西南木荷 Schima wallichii	1 195.06	22.49	911.85
	泥柯 Lithocarpus fenestratus	934.20	91.60	2075.46
	截果柯 L. truncatus	977.11	21.45	729.09
	合计 Total	1 051.94	32.98	2075.46

参数 Parameter	生长阶段 Growth stages	平均值 Mean	变异系数 Coefficient of variation (%)	极差 Range
N	幼苗幼树 Seedling	12.92	22.45	12.27
	小树 Sapling	12.96	29.08	16.89
	中树 Medium tree	13.94	22.83	12.29
	大树 Mature tree	12.87	16.72	6.96
P	幼苗幼树 Seedling	0.45	35.22	0.76
	小树 Sapling	0.53	44.66	1.01
	中树 Medium tree	0.66	72.98	1.61
	大树 Mature tree	1.01	65.71	1.53
C	幼苗幼树 Seedling	484.09	5.06	77.23
	小树 Sapling	487.59	4.15	74.98
	中树 Medium tree	488.38	5.11	107.37
	大树 Mature tree	489.16	2.88	42.31
C:N	幼苗幼树 Seedling	39.77	30.18	58.16
	小树 Sapling	40.97	32.30	53.72
	中树 Medium tree	37.24	28.78	41.14
	大树 Mature tree	39.19	21.22	26.09
N:P	幼苗幼树 Seedling	33.39	54.41	63.98
	小树 Sapling	26.22	30.26	31.49
	中树 Medium tree	26.44	38.23	34.00
	大树 Mature tree	19.86	64.21	26.90
C:P	幼苗幼树 Seedling	1 225.22	38.58	1 808.29
	小树 Sapling	1 023.82	27.24	1 094.90
	中树 Medium tree	954.83	36.11	1 209.56
	大树 Mature tree	803.67	74.58	1 647.69