Chin J Plan Ecolo ›› 2011, Vol. 35 ›› Issue (7): 769-778.doi: 10.3724/SP.J.1258.2011.00769

• Research Articles • Previous Articles     Next Articles

Effect of high temperature on leaf senescence and related enzymes of grain starch synthesis in stay-green wheat after anthesis

SHI Hui-Qing, GONG Yue-Hua*, and ZHANG Dong-Wu   

  1. College of Life Sciences, Northwest Sci-Tech University of Agriculture & Forestry, Yangling, Shaanxi 712100, China
  • Received:2011-02-21 Revised:2011-05-06 Online:2011-08-18 Published:2011-07-01
  • Contact: GONG Yue-Hua E-mail:gongyh01@163.com

Abstract:

Aims Our objectives were to explore the influence of high temperature on enzymes of grain starch synthesis in stay-green wheat (Triticum aestivum) and reveal heat tolerance in stay-green wheat.
Methods We used the stay-green wheat ‘Wm8’ and ‘Ym66’ and the control varieties ‘XY6’ and ‘XY22’ as experimental materials. A warming cover of plastic film was used to carry out the high-temperature treatment. After anthesis, we measured green leaf number, chlorophyll content, malonaldehyde (MDA) and membrane permeability, as well as the activities of the enzymes of grain starch synthesis were measured.
Important findings High temperature after anthesis promotes rapid leaf senescence. For all four varieties, the green leaf number and chlorophyll content significantly decreased, but the MDA content and the relative electric conductivity increased markedly. The stay-green wheat varieties had higher green leaf number and chlorophyll content and lower MDA content and relative electric conductivity than the control cultivars after high temperature treatment. In grains, the activities of the enzymes of grain starch synthesis (sucrose synthase, SS; adenosine diphosphate glucose pyrophosphorylase, AGPP; and soluble starch synthase, SSS) in all four varieties decreased after high temperature treatment. But activities of these enzymes in the stay-green wheat were also higher than those in the control wheat, suggesting that it was beneficial for the starch accumulation in grain of stay-green wheat under high temperature. Among the three enzymes, a significant difference existed in SSS activity between the high temperature treatment and the control, and activities of the above three enzymes were positively and significantly correlated with grain-filling rate (r = 0.905, 0.419 and 0.801, respectively), so they play a key role in starch synthesis in the grain of wheat. Above all, the stay-green wheats have better resistance to high temperature than other varieties, and they have strong photosynthesis capacity and higher grain weight. Hence, breeding new cultivars with the stay-green characteristic would reduce the influence of high temperature stress during grain filling in wheat production.

Tian LC(田良才), Zhang MY(张明仪), Li JC(李晋传) (1995).Hhigh temperature stress: main barrier of high productivity on wheat. Shanxi Agricultural Sceiences (山西农业科学),23:14-18. (in Chinese with English abstract).
Wardlaw IF (1980).Factors limiting the rate of dry matter accumulation in the grain of wheat grown at high temperature. Aust Physiol, 7:387-400.
Li YG(李永庚), Yu ZW(于振文), Zhang XJ(张秀杰) (2005). Response of yield and quality of wheat to heat stress at qifferent grain filling stages. Acta Phytoecol Sinica (植物生态学报), 29(3):164-466. (in Chinese with English abstract).
Wiegand CL (1981). Cuellar J A.Duration of grain filling and kernel weight of wheat of wheat as affected by temperyure.Crop Science, 21:95-101.
Yan CS(闫长生), Xiao SH(肖世和), Zhang XY(张秀英) (2003). Evaluation of heat tolerance during maturity in winter wheat. Acta Agriculturae Boreali-Sinica (华北农学报), 18(3):15-17. (in Chinese with English abstract).
Wu YS(武永胜), Xue H(薛晖), Liu Y(刘洋), Gong YH(龚月桦) (2010). Study on Senescence and Chlorophyll Fluorescence Traits of Stay-green Leaf in Wheat. Agricultural Research in the AridAreas.(干旱地区农业研究),28(4):117-127. (in Chinese with English abstract).
Li YG(李永庚), Jiang GM(蒋高明), Yang JC(杨景成) (2003). Effects of temperature on carbon and nitrogen metabolism yield and quality of wheat. Acta Phytoecologica Sinica (植物生态学报),27(2):164-169. (in Chinese with English abstract).
Pan QM(潘庆民),Han XG(韩兴国),Bai YF(白永飞),Yang JC(杨景成) (2002). Advances in physiology and ecology studies on stored carbohydrates in plants.Chinese Bulletin of Botany (植物学通报) ,19(1):30-38. (in Chinese with English abstract).
Smith,AM, Denyer K. & Martin CR (1995). What controls the amount and structure of starch in store organ. Plant Physiology, 95:673-680.
Schaffer AA.& Petreikov M (1997). Sucrose-to-starch metabolism tomato fruit undergoing transient starch accumulation. Plant Physiology,113:739-746.
Liang JS(梁建生), Cao XZ(曹显祖), Xu S(徐生) (2004).The relationship between Source-sink intensity and starch accumulation during grain filling period in two winter wheat cuitivars with different spike types.Acta Agronomica Sinica (作物学报), 9:678-691. (in Chinese with English abstract).
Li YG(李永庚), Yu ZW(于振文), Jiang D(姜冬),Yu S L(余松烈) (2001). Studies on the dynamic changes of the synthesis of sucrose in the flag leaf and starch in the grain of high-yielding wheat, and those of the enzymes involved. Acta Agronomica Sinica (作物学报), 27:658-664. (in Chinese with English abstract).
Xu XL,Wang Z M,Zhang J P (2001). Effects of heat strees on photosynthetic characteristics of different green organs of winter wheat during grain filling stage. AetaBotany Sinica, 43 (6):571-577.
Zhang X Z (1985). Study on determination of chlorophyⅡ content of laeves.Liaoning Agricultural Sceiences (辽宁农业科学), 8:26-29.
Sun Q(孙群),Hu JJ(胡景江) (2006).Research technology of plant physiology(植物生理学研究技术技术), Northwest A&F Sci-Tech University Press, ShanXi.(in Chinese).
Gao JF(高俊凤), Sun Q(孙群), Liang ZS(梁宗锁) (2000). Experimental techniques in plant physiology(植物生理学实验技术), World Book Publishing Company,Xi’an. (in Chinese).
Douglas CD, Kuo TM,Felker FC (1988). Emymes of sucrose and hexose metabolism in developing kernels of two inbreds of maize.Plant Physiology, 86:1013-1019.
Gao JF(高俊凤), Gong YH(龚月桦) (2006). Experimental Introduction of plant physiology (植物生理学实验指导), Higher Education Press, Beijing. (in Chinese).
Yu XJ(於新建) (1999). Shanghai Plant physiol. Inst.,CAS,Shanghai PlantPhysiol.Association. Introduction tomodel plant physiological experiments.Beijing:Science Press, (in Chinese).
Nakamura Y, Yuki K, Park SY, Ohya T (1989). Carbohydrate metabolism in the developing endosperm of rice grains. Plant Cell Physiology,30(6):833-839.
Cheng FM(程方明), Jiang DA(蒋德安), Wu P(吴平), Shi CH(石春海) (2001).The Dynamic Change of Starch Synthesis Enzymes during the Grain Filling Stage and Effects of Temperature upon It. Acta Agronomica Sinica (作物学报), 27(2):201-206. (in Chinese with English abstract).
Ma XD(马晓娣),Wang L(王丽),Wang M(汪矛),Peng HR(彭惠茹) (2003). Difference in relative conductivity and utrastructure of leaf between two wheat cultivars with different thermo
tolerance under heat acclimation and heat stress. Journal of China Agricultural University (中国农业大学学报),8(5):4-8. (in Chinese with English abstract).
Xu RQ(徐如强),Sun QX(孙其信), Zhang SZ(张树榛) (1998).Current status and prospective on the investigation of heat tolerance in wheat. Journal of China Agricultural University (中国农业大学学报), 3(3):33-40. (in Chinese with English abstract).
Rehman H, Malik S A., Saleem M (1985). Heat tolerance of upland cotton during the fruiting stage evaluated during cellular membrane thermostability.Field Crops Res. 149~158.
Bekavac G(1998).Path analysis of stay-green trait in maize.Cereal researchcommunications, 26(2): 1661-1671.
Liu KC(刘开昌), Wang QC(王庆成), Zhang HS(张海松) (2003). Advances in heredity characteristics and physiological mechanism of stay-green in maize leaves.Shandong Agricultural Sceiences(山东农业科学),(2):47-51. (in Chinese with English abstract).
Li SQ(李世清), Shao MA(邵明安), Li ZY(李紫燕), Wu WM(伍维模), Zhang XC(张兴昌) (2003). Review of characteristics of wheat grain fill and factors to influence it. Acta botanica Boreali-Occidentalia Sinica(西北植物学报), 23:2031-2039. (in Chinese with English abstract).
Yan SH(闫素辉), Yin YP(尹燕枰), Li WY(李文阳), Li Y(李勇) Liang TB(梁太波), Wu Yh(邬云海), Geng QH(耿庆辉),Wang ZL(王振林) (2008) Effect of temperature during grain filling on starch accumulation,starch granule distribution, and activities of related enzymes in wheat grain. Acta Agronomica Sinica (作物学报), 28(12):2138-2146. (in Chinese with English abstract).
Keeling PL, Bacon PJ, Holt DC (1993). Elevation temperature reduces starch deposition in wheat endosperm by reducing the activity of soluble starch synthase. Planta,191:341-348.
Jenner CF, Siwek K, Hawker JS (1993). The synthesis of 14C starch from 14C sucrose in isolated wheat grain is dependent upon the activity of soluble starch synthase. Australian Journal of Plant Physiology,20:329-335.
Zhang YH(张英华), Wang ZM(王志敏) (2006).The effect of heat stress on grain growth in the growing period of wheat. Chinese Journal of Eco-Agriculture (中国生态农业报) ,14(3):8-11. (in Chinese with English abstract).
Chen XY(陈希勇), Sun QX(孙其信), Sun CZ(孙长征) (2000). Performance and evaluation of spring wheat heat tolerance.Journal of Agricultural university (中国农业大学学报), 5:1-8. (in Chinese with English abstract).
No related articles found!
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] Yan Xiao-hua Cai Zhu-ping. Effects of S-07, PP333 and Triadimefon on Peroxidaseisoentyme of Rice Seedling[J]. Chin Bull Bot, 1995, 12(专辑3): 109 -112 .
[2] . [J]. Chin Bull Bot, 1994, 11(专辑): 13 .
[3] Xiaomin Yu;Xingguo Lan;Yuhua Li. The Ub/26S Proteasome Pathway and Self-incompatible Responses in Flowering Plants[J]. Chin Bull Bot, 2006, 23(2): 197 -206 .
[4] WANG Ling-Li LIU Wen-Zhe. Contents of Camptothecin in Camptotheca acuminata from Different Provenances[J]. Chin Bull Bot, 2005, 22(05): 584 -589 .
[5] Dai Yun-ling and Xu Chun-hui. Advances in Research on Protein Components of Oxygen-evolving Complex[J]. Chin Bull Bot, 1992, 9(03): 1 -16 .
[6] . Advances in Research on Photosynthesis of Submerged Macrophytes[J]. Chin Bull Bot, 2005, 22(增刊): 128 -138 .
[7] Shaobin Zhang;Guoqin Liu. Research Advances in Plant Actin Isoforms[J]. Chin Bull Bot, 2006, 23(3): 242 -248 .
[8] BU Ren-Cang, CHANG Yu, HU Yuan-Man, LI Xiu-Zhen, HE Hong-Shi. SENSITIVITY OF CONIFEROUS TREES TO ENVIRONMENTAL FACTORS AT DIFFERENT SCALES IN THE SMALL XING’AN MOUNTAINS, CHINA[J]. Chin J Plan Ecolo, 2008, 32(1): 80 -87 .
[9] MA Li-Hui, WU Pu-Te, and WANG You-Ke. Spatial pattern of root systems of dense jujube plantation with jujube age in the semiarid loess hilly region of China[J]. Chin J Plan Ecolo, 2012, 36(4): 292 -301 .
[10] PAN Yu-De, Melillo J. M., Kicklighter D. W., XIAO Xiang-Ming, McGuire A. D.. Modeling Structural and Functional Responses of Terrestria Ecosystems in China to Changes in Climate and Atmospheric CO2[J]. Chin J Plan Ecolo, 2001, 25(2): 175 -189 .