NaCl胁迫加重强光胁迫下超大甜椒叶片的光系统II和光系统I的光抑制

doi:10.3724/SP.J.1258.2011.00681

植物生态学报 ›› 2011, Vol. 35 ›› Issue (6): 681-686.DOI: 10.3724/SP.J.1258.2011.00681

• 研究论文 • 上一篇

NaCl胁迫加重强光胁迫下超大甜椒叶片的光系统II和光系统I的光抑制

宋旭丽¹^,²^,³^,⁴, 胡春梅¹^,², 孟静静³^,⁴, 侯喜林¹^,²^,^*(), 何启伟³, 李新国³^,⁴^,^*()

¹南京农业大学作物遗传与种质创新国家重点试验室, 南京 210095
²农业部南方蔬菜遗传改良重点开放试验室, 南京 210095
³山东省农业科学院高新技术研究中心和山东省作物与畜禽品种改良生物技术重点试验室, 济南 250100
⁴农业部黄淮海作物遗传改良与生物技术重点开放试验室, 济南 250100

收稿日期:2010-11-26 接受日期:2010-01-28 出版日期:2011-11-26 发布日期:2011-06-30
通讯作者: 侯喜林,李新国
作者简介:lixinguo@tom.com
* E-mail: hxl@njau.edu.cn;

NaCl stress aggravates photoinhibition of photosystem II and photosystem I in Capsicum annuum leaves under high irradiance stress

SONG Xu-Li¹^,²^,³^,⁴, HU Chun-Mei¹^,², MENG Jing-Jing³^,⁴, HOU Xi-Lin¹^,²^,^*(), HE Qi-Wei³, LI Xin-Guo³^,⁴^,^*()

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China
²Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, China
³High-Tech Research Center, Shandong Academy of Agricultural Sciences and Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Jinan 250100, China
⁴Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China

Received:2010-11-26 Accepted:2010-01-28 Online:2011-11-26 Published:2011-06-30
Contact: HOU Xi-Lin,LI Xin-Guo

摘要/Abstract

摘要：

快速叶绿素荧光动力学可以在无损情况下探知叶片光合机构的损伤程度, 快速叶绿素荧光测定和分析技术(JIP-test)将测量值转化为多种具有生物学意义的参数, 因而被广泛应用于植物光合机构对环境的响应机制研究。该文研究了超大甜椒(Capsicum annuum)幼苗在强光及不同NaCl浓度胁迫下的荧光响应情况。与单纯强光胁迫相比, NaCl胁迫引起了叶绿素荧光诱导曲线的明显改变, 光系统II (PSII)光抑制加重, 同时PSII反应中心和受体侧受到明显影响, 而且高NaCl浓度胁迫下PSII供体侧受伤害明显, 同时PSI反应中心活性(P700⁺)在盐胁迫下明显降低。这些结果表明, NaCl胁迫会增强强光对超大甜椒光系统的光抑制, 并且浓度越高抑制越明显, 但对PSI的抑制作用低于PSII。高NaCl浓度胁迫易对PSII供体侧造成破坏, 且PSI光抑制严重。

关键词: 强光胁迫, 光抑制, 光系统, 盐胁迫, 超大甜椒

Abstract:

Aims Our aim was to study effects of salt stress and high irradiance on photoinhibition of photosystem II (PSII) and I (PSI) in leaves of sweet pepper (Capsicum annuum).

Methods We used the chlorophyll a fluorescence technique and the JIP-test to study the responses of sweet pepper leaves to salt stress with different NaCl concentrations and under high irradiance (1 000 μmol·m^-2·s^-1).

Important findings The chlorophyll a fluorescence induction curves changed greatly as induced by NaCl stress under high irradiance, and photoinhibition of PSII was aggravated with apparent effects on PSII reaction centers and the acceptor side. Also, the donor side of PSII was damaged by high NaCl concentration under high irradiance. Additionally, the activity of PSI reaction centers (P700⁺) was inhibited by NaCl stress under high irradiance. Therefore, NaCl stress could aggravate photoinhibition of sweet pepper photosystem under high irradiance, and photoinhibition was greater with higher NaCl concentration. However, PSI photoinhibition was less than PSII photoinhibition. Additionally, high NaCl concentration could induce both damage to PSII donor sides and PSI.

Key words: high irradiance stress, photoinhibition, photosystem, salt stress, sweet pepper

宋旭丽, 胡春梅, 孟静静, 侯喜林, 何启伟, 李新国. NaCl胁迫加重强光胁迫下超大甜椒叶片的光系统II和光系统I的光抑制. 植物生态学报, 2011, 35(6): 681-686. DOI: 10.3724/SP.J.1258.2011.00681

SONG Xu-Li, HU Chun-Mei, MENG Jing-Jing, HOU Xi-Lin, HE Qi-Wei, LI Xin-Guo. NaCl stress aggravates photoinhibition of photosystem II and photosystem I in Capsicum annuum leaves under high irradiance stress. Chinese Journal of Plant Ecology, 2011, 35(6): 681-686. DOI: 10.3724/SP.J.1258.2011.00681

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2011.00681

https://www.plant-ecology.com/CN/Y2011/V35/I6/681

图/表 4

图1 强光下NaCl胁迫对超大甜椒叶片快速叶绿素荧光动力学曲线的影响。T250是第3天时的数据, 其他胁迫均为第7天的数据。T0、T50、T150、T250, NaCl浓度分别为0、50、150、250 mmol·L-1。

Fig. 1 Effects of NaCl stress on the chlorophyll a fluorescence transient curve in sweet pepper leaves under high irradiance. The data of T250 was on the 3rd day, and the data of the other stresses were on the 7th day. T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

图2 强光下NaCl胁迫对超大甜椒叶片PSII最大光化学效率(A)、300 μs时的相对荧光(B)、2 ms时的相对荧光(C)和反应中心捕获的激子能将电子通过QA传递到其他电子受体的概率(D)的影响(平均值±标准偏差)。T0、T50、T150、T250, NaCl浓度分别为0、50、150、250 mmol·L-1。

Fig. 2 Effects of NaCl stress on φP0 (A), VK (B), Vj (C) andΨ0 (D) in sweet pepper leaves under high irradiance (mean ± SD). φPo, maximum quantum yield for primary photochemistry; Ψ0, the efficiency by which a absorbed photon in the antennae moves an electron into the electron transport chain beyond QA-; Vj, relative variable fluorescence at the J-step; Vk, relative variable fluorescence intensity at 300 μs. T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

图3 强光下NaCl胁迫对超大甜椒叶片单位面积有活性的PSII反应中心的数量(RC/CSm) (A)、单位面积吸收的光能(ABS/CSm) (B)、单位面积捕获的光能(TR/CSm) (C)和单位面积用于电子传递的能量(ET/CSm) (D)的影响(平均值±标准偏差)。T0、T50、T150、T250, NaCl浓度分别为0、50、150、250 mmol·L-1。

Fig. 3 Effects of NaCl stress on RC/CSm (A), ABS/CSm (B), TR/CSm (C) and ET/CSm (D) in sweet pepper leaves under high irradiance (mean ± SD). ABS/CSm, absorption flux per excited cross section; ET/CSm, electron transport flux per cross section; RC/CSm, density of PSII active reaction centers per excited cross-section; TR/CSm, trapped energy flux per cross section. T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

图4 强光下NaCl胁迫对超大甜椒叶片PSI反应中心活性(P700+)的影响(平均值±标准偏差)。T0、T50、T150、T250, NaCl浓度分别为0、50、150、250 mmol·L-1。

Fig. 4 Effects of NaCl stress on the activity of PSI (P700+) in sweet pepper leaves under high irradiance (mean ± SD). T0, T50, T150 and T250 represents the seedlings was treated with 0, 50, 150 and 250 mmol·L-1 NaCl, respectively.

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NaCl胁迫加重强光胁迫下超大甜椒叶片的光系统II和光系统I的光抑制

NaCl stress aggravates photoinhibition of photosystem II and photosystem I in Capsicum annuum leaves under high irradiance stress

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