He-Ne激光处理与干旱胁迫对小麦幼苗保护酶活性及脂质过氧化作用的影响

doi:10.3773/j.issn.1005-264x.2008.05.004

摘要/Abstract

摘要：

用He-Ne激光(5.23 mW·mm^-2)处理经5%、10%、15% PEG6000胁迫的小麦幼苗, 分析了干旱胁迫条件下激光处理对小麦幼苗保护酶活性及脂质过氧化作用的影响。适度干旱胁迫的小麦幼苗经He-Ne激光辐照后, 丙二醛(MDA)含量和超氧自由基(O₂^-.)产生速率显著降低(p<0.05), 而过氧化物酶(POD)活性和抗坏血酸(AsA)、谷胱甘肽(GSH)含量却显著增加(p<0.05)。总体上看, 5%和10% PEG6000胁迫的小麦幼苗经激光辐照3 min后抗旱性增强。

关键词: He-Ne激光, 小麦, 干旱胁迫, 保护酶, 脂质过氧化

Abstract:

Aims We studied protective enzyme activities and lipid peroxidation in wheat seedlings treated with different concentration of PEG6000 to determine the effects of He-Ne laser on wheat (Triticum aestivum)exposed to drought stress.

Method We treated 12-day-old, drought-stressed seedlings with 5%, 10% and 15% PEG6000 for 3, 6, 9, 12 and 15 days. After 3, 6, 9, 12 and 15 days of drought stress, a He-Ne laser was employed to irradiate seedlings of spring wheat for 0, 1 and 3 min. Leaves were sampled for various analyses.

Important findings He-Ne laser irradiation significantly decreased malondialdehyde (MDA) content and superoxide radical (O₂^-.) production rate and notably increased peroxidase (POD) enzyme activity and ascorbate (AsA) and glutathione (GSH) concentration in wheat seedlings exposed to moderate drought stress. We concluded that 3 min laser irradiation enhanced anti-drought characteristics of wheat seedlings treated with 5% or 10% PEG6000.

Key words: He-Ne laser, wheat, drought stress, protective enzyme, lipid peroxidation

邱宗波, 刘晓, 李方民, 田向军, 岳明. He-Ne激光处理与干旱胁迫对小麦幼苗保护酶活性及脂质过氧化作用的影响. 植物生态学报, 2008, 32(5): 1002-1006. DOI: 10.3773/j.issn.1005-264x.2008.05.004

QIU Zong-Bo, LIU Xiao, LI Fang-Min, TIAN Xiang-Jun, YUE Ming. INFLUENCE OF He-Ne LASER IRRADIATION ON PROTECTIVE ENZYME ACTIVITIES AND LIPID PEROXIDATION IN WHEAT SEEDLINGS BY DROUGHT STRESS DAMAGE. Chinese Journal of Plant Ecology, 2008, 32(5): 1002-1006. DOI: 10.3773/j.issn.1005-264x.2008.05.004

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.05.004

https://www.plant-ecology.com/CN/Y2008/V32/I5/1002

图/表 5

参考文献 9

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PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15
0	0	22.58±1.62	27.10±2.05	30.13±3.62	34.51±3.62	32.68±3.06
5	0	31.61±2.85^a	49.70±2.67^a	52.69±17.10^a	54.76±4.52^a	54.19±9.64^a
5	1	30.11±2.61^a	49.68±4.57^a	52.73±13.79^a	53.63±2.67^a	53.38±2.67^a
5	3	28.09±2.65^a	48.71±3.89^a	51.18±10.43^a	53.19±7.82^a	51.29±4.52^a
10	0	34.62±11.37^a	52.69±9.40^a	52.72±11.37^a	56.73±2.67^a	62.76±5.21^a
10	1	30.60±2.61^a	49.68±4.51^a	48.17±2.61^a	55.27±5.25^a	58.71±4.51^a
10	3	23.08±2.61^b	44.19±5.64^b	42.15±2.67^b	44.73±2.67^b	49.14±2.67^b
15	0	35.59±2.67^a	54.63±2.67^a	54.65±4.51^a	58.17±2.67^a	65.23±7.82^a
15	1	34.59±6.89^a	55.16±6.98^a	54.68±7.82^a	56.67±2.67^a	66.20±9.41^a
15	3	37.09±8.72^a	54.70±6.89^a	57.66±6.89^a	59.68±4.57^a	67.22±18.25^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15
0	0	22.58±1.62	27.10±2.05	30.13±3.62	34.51±3.62	32.68±3.06
5	0	31.61±2.85^a	49.70±2.67^a	52.69±17.10^a	54.76±4.52^a	54.19±9.64^a
5	1	30.11±2.61^a	49.68±4.57^a	52.73±13.79^a	53.63±2.67^a	53.38±2.67^a
5	3	28.09±2.65^a	48.71±3.89^a	51.18±10.43^a	53.19±7.82^a	51.29±4.52^a
10	0	34.62±11.37^a	52.69±9.40^a	52.72±11.37^a	56.73±2.67^a	62.76±5.21^a
10	1	30.60±2.61^a	49.68±4.51^a	48.17±2.61^a	55.27±5.25^a	58.71±4.51^a
10	3	23.08±2.61^b	44.19±5.64^b	42.15±2.67^b	44.73±2.67^b	49.14±2.67^b
15	0	35.59±2.67^a	54.63±2.67^a	54.65±4.51^a	58.17±2.67^a	65.23±7.82^a
15	1	34.59±6.89^a	55.16±6.98^a	54.68±7.82^a	56.67±2.67^a	66.20±9.41^a
15	3	37.09±8.72^a	54.70±6.89^a	57.66±6.89^a	59.68±4.57^a	67.22±18.25^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15	12d	15d
0	0	1.61±0.62	2.44±0.35	3.12±0.38	3.83±0.28	3.52±0.26
5	0	3.22±0.54^a	3.08±0.23^a	4.50±0.10^a	4.68±0.09^a	4.06±0.80^a
5	1	2.85±1.08^a	2.89±0.06^a	4.26±0.50^a	4.41±0.30^a	3.69±0.52^a
5	3	1.04±0.13^b	2.61±0.31^b	2.64±0.60^b	3.65±0.80^b	3.02±0.14^b
10	0	3.45±0.27^a	3.48±0.09^a	4.83±0.30^a	5.05±0.40^a	4.85±0.40^a
10	1	3.27±0.95^a	3.23±0.09^a	4.28±0.15^a	4.89±0.08^a	4.61±0.58^a
10	3	3.10±0.59^a	3.11±0.04^a	4.22±0.41^a	4.72±0.48^a	4.49±0.04^a
15	0	3.48±1.49^a	4.21±0.21^a	4.68±0.53^a	5.62±0.34^a	5.55±0.48^a
15	1	3.13±0.54^a	4.06±0.56^a	4.86±0.10^a	5.69±0.28^a	5.60±0.52^a
15	3	3.29±0.82^a	4.05±0.06^a	4.71±0.72^a	5.49±0.62^a	5.49±0.82^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15	12d	15d
0	0	1.61±0.62	2.44±0.35	3.12±0.38	3.83±0.28	3.52±0.26
5	0	3.22±0.54^a	3.08±0.23^a	4.50±0.10^a	4.68±0.09^a	4.06±0.80^a
5	1	2.85±1.08^a	2.89±0.06^a	4.26±0.50^a	4.41±0.30^a	3.69±0.52^a
5	3	1.04±0.13^b	2.61±0.31^b	2.64±0.60^b	3.65±0.80^b	3.02±0.14^b
10	0	3.45±0.27^a	3.48±0.09^a	4.83±0.30^a	5.05±0.40^a	4.85±0.40^a
10	1	3.27±0.95^a	3.23±0.09^a	4.28±0.15^a	4.89±0.08^a	4.61±0.58^a
10	3	3.10±0.59^a	3.11±0.04^a	4.22±0.41^a	4.72±0.48^a	4.49±0.04^a
15	0	3.48±1.49^a	4.21±0.21^a	4.68±0.53^a	5.62±0.34^a	5.55±0.48^a
15	1	3.13±0.54^a	4.06±0.56^a	4.86±0.10^a	5.69±0.28^a	5.60±0.52^a
15	3	3.29±0.82^a	4.05±0.06^a	4.71±0.72^a	5.49±0.62^a	5.49±0.82^a

PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	PEG胁迫处理天数 Time of drought stress (d)
PEG浓度 PEG content (%)	激光处理时间Treatment time of laser (min)	3	6	9	12	15	9d	12d	15d
0	0	8.40±0.85	9.42±0.65	10.21±1.28	10.43±2.65	11.27±2.36
5	0	8.53±0.92^b	8.13±0.58^b	10.47±1.61^b	11.20±1.72^b	15.13±1.31^b
5	1	8.93±1.36^b	8.60±0.53^b	10.97±1.49^b	12.47±2.39^b	16.20±1.89^a
5	3	10.93±1.64^a	12.60±1.72^a	13.23±1.44^a	14.33±1.98^a	17.53±1.77^a
10	0	10.30±1.90^a	11.47±1.61^a	13.57±1.63^a	14.47±1.12^a	24.87±1.66^a
10	1	12.40±1.60^a	11.40±1.59^a	13.80±1.58^a	16.20±1.87^a	26.00±1.58^a
10	3	10.53±1.64^a	11.53±1.57^a	13.07±1.15^a	15.40±1.72^a	25.07±1.67^a
15	0	12.70±1.46^a	12.10±1.75^a	12.73±1.64^a	12.40±1.02^a	18.00±2.27^a
15	1	12.00±1.87^a	12.60±2.86^a	13.73±0.95^a	13.27±2.53^a	18.67±1.45^a
15	3	12.27±1.60^a	12.80±1.58^a	12.80±1.20^a	13.87±1.92^a	18.53±1.84^a