氮磷水平对龙须菜生长和光合特性的影响

doi:10.3773/j.issn.1005-264x.2009.06.014

植物生态学报 ›› 2009, Vol. 33 ›› Issue (6): 1140-1147.DOI: 10.3773/j.issn.1005-264x.2009.06.014

氮磷水平对龙须菜生长和光合特性的影响

李枫¹, 邹定辉²^,³, 刘兆普¹^,^*(), 赵耕毛¹, 程丽巍¹, 朱喜锋³, 陈伟洲³

1 南京农业大学资源与环境科学学院, 江苏省海洋生物学重点实验室,南京 210095
2 华南理工大学环境科学与工程学院,广州 510006
3 汕头大学海洋生物研究所,广东汕头 515063

收稿日期:2009-01-07 接受日期:2009-04-23 出版日期:2009-01-07 发布日期:2021-04-29
通讯作者: 刘兆普
作者简介:*(sea@njau.edu.cn）
基金资助:
国家自然科学基金(30670396);国家自然科学基金(30470331);江苏省海洋生物学重点实验室开放课题(JSMK2008-003)

EFFECTS OF NITROGEN AND PHOSPHOROUS LEVELS ON GROWTH AND PHOTOSYNTHETIC TRAITS OF GRACILARIA LEMANEIFORMIS (RHODOPHYTA)

LI Feng¹, ZOU Ding-Hui²^,³, LIU Zhao-Pu¹^,^*(), ZHAO Geng-Mao¹, CHENG Li-Wei¹, ZHU Xi-Feng³, CHEN Wei-Zhou³

1 College of Resources and Environmental Sciences, Nanjing Agricultural University, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing 210095, China
2 College of Environmental Science and Engineering, Guangzhou 510006, China
3 Marine Biology Institute, Shantou University, Shantou, Guangdong 515063, China

Received:2009-01-07 Accepted:2009-04-23 Online:2009-01-07 Published:2021-04-29
Contact: LIU Zhao-Pu

摘要/Abstract

摘要：

研究不同营养盐条件对龙须菜(Gracilaria lemaneiformis)的生理效应, 对深入了解龙须菜与近海环境的相互作用具有重要意义。在低氮低磷(LNLP)、低氮高磷(LNHP)、高氮低磷(HNLP)和高氮高磷(HNHP) 4种营养盐条件下培养龙须菜15 d, 以探讨不同氮、磷水平对龙须菜生长和光合特性的影响。结果表明: 1) LNHP、HNLP和HNHP处理促进了龙须菜的生长, 其中HNHP处理下龙须菜具有最大的相对生长速率和生物量; 2) LNHP、HNLP和HNHP处理提高了龙须菜的光合无机碳利用能力, 其中HNHP处理下龙须菜具有最大的无机碳饱和光合速率和表观半饱和常数, 比LNLP处理分别提高了118%倍和48.71%; 3) LNHP、HNLP和HNHP处理显著影响龙须菜的光化学效率, 与LNLP处理相比, LNHP处理提高了龙须菜的光化学效率, 而HNLP和HNHP处理降低了龙须菜的光化学效率。研究结果表明, HNHP处理条件下, 龙须菜的生长和光合无机碳利用能力最高, 光化学效率最低。

关键词: 龙须菜, 氮, 磷, 生长, 光合作用, 光化学效率

Abstract:

Aim Our objective was to investigate the impacts of different nutrient levels on the physiology of Gracilaria lemaneiformis to evaluate the relationship between this mariculture species and costal environment.
Methods Thalli of Gracilaria lemaneiformis were cultured for 15 days under four different nutrient conditions (low N and low P, low N and high P, high N and low P, and high N and high P) to examine the possible effects of the availability of different nutrients on growth and photosynthesis in this alga.
Important findings Growth of G. lemaneiformis was enhanced with the low N and high P treatment, high N and low P treatment, and high N and high P treatment, compared to low N and low P treatment. The highest relative growth rate (RGR) and biomass were observed under high N and high P treatment.Gracilaria lemaneiformiswas capable of using HCO₃^-as a source of exogenous inorganic carbon (Ci) for its photosynthesis, and the ability was increased at the above three nutrient conditions. Both carbon-saturated photosynthetic rate and apparent half saturation constant under high N and high P treatment were significant higher than those under low N and low P treatment by 118% and 48.71%, respectively. Photochemical efficiency of G. lemaneiformis was stimulated with low N and high P treatment, but was inhibited at the two high N treatments. Thus, the rates of growth and photosynthesis were the highest, but the photochemical efficiency was the lowest inG. lemaneiformis grown under high N and high P treatment.

Key words: Gracilaria lemaneiformis, nitrogen, phosphorus, growth, photosynthesis, photochemical efficiency

李枫, 邹定辉, 刘兆普, 赵耕毛, 程丽巍, 朱喜锋, 陈伟洲. 氮磷水平对龙须菜生长和光合特性的影响. 植物生态学报, 2009, 33(6): 1140-1147. DOI: 10.3773/j.issn.1005-264x.2009.06.014

LI Feng, ZOU Ding-Hui, LIU Zhao-Pu, ZHAO Geng-Mao, CHENG Li-Wei, ZHU Xi-Feng, CHEN Wei-Zhou. EFFECTS OF NITROGEN AND PHOSPHOROUS LEVELS ON GROWTH AND PHOTOSYNTHETIC TRAITS OF GRACILARIA LEMANEIFORMIS (RHODOPHYTA). Chinese Journal of Plant Ecology, 2009, 33(6): 1140-1147. DOI: 10.3773/j.issn.1005-264x.2009.06.014

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.06.014

https://www.plant-ecology.com/CN/Y2009/V33/I6/1140

图/表 5

图1 不同浓度N、P组合处理下龙须菜的相对生长速率(RGR)和生产力 LNLP: 低氮低磷, 即过滤自然海水中不添加N与P Filtered natural seawater LNHP: 低氮高磷, 即过滤自然海水中添加40 μmol·L -1NaH2PO4 Filtered natural seawater add 40 μmol·L -1 NaH2PO4 HNLP: 高氮低磷, 在过滤自然海水中添加160 μmol·L -1 NaNO3, 40 μmol·L -1 NH4Cl Filtered natural seawater add 160 μmol·L -1 NaNO3 and 40 μmol·L -1NH4Cl HNHP: 高氮高磷, 在过滤自然海水中添加160 μmol·L -1 NaNO3、40 μmol·L -1NH4Cl和40 μmol·L -1NaH2PO4Filtered natural seawater add 160 μmol·L -1 NaNO3, 40 μmol·L -1NH4Cl and 40 μmol·L -1NaH2PO4

Fig. 1 Relative growth rate (RGR) and productivity of Gracilaria lemaneiformis under different nutrients treatments

图2 不同浓度N、P组合处理下龙须菜光合放氧对无机碳浓度的响应曲线 LNLP, LNHP, HNLP, HNHP同图1 See Fig. 1

Fig. 2 Rates of photosynthetic oxygen evolution vs. inorganic carbon concentration curves of Gracilaria lemaneiformis under different nutrients treatments

表1 不同浓度N、P组合处理下龙须菜光合放氧对无机碳浓度的响应曲线的动力学参数

Table 1 Kinetic parameters in the curves of rates of photosynthetic oxygen evolution vs. Ci concentration of Gracilaria lemaneiformis under different nutrients treatments

动力学参数		LNLP		LNHP		HNLP		HNHP
无机碳饱和光合速率 Carbon-saturating maximum photosynthetic rate (V_max) (μmol O₂·g^-1FW·h^-1)		29.88±8.55^a		51.34±2.88^bc		43.28±6.15^ac		65.23±12.11^b
表观半饱和常数 The concentration of inorganic carbon (K_m) (mmol·L^-1)		3.49±1.12^a		4.15±0.44^ab		2.96±0.27^a		5.19±0.99^b

图3 不同浓度N、P组合处理下龙须菜的光化学效率 (A) PSII有效量子产量 Effective quantum yield of PSII (B) 相对电子传递速率-光照强度响应曲线 Relative electron transport rates (rETR) vs. irradiance curves LNLP, LNHP, HNLP, HNHP同图1 See Fig. 1

Fig. 3 Photochemical efficiency of Gracilaria lemaneiformis under different nutrients treatments

表2 不同浓度N、P组合处理下龙须菜的相对电子传递速率-光照强度响应曲线的光合参数

Table 2 Photosynthetic parameters in the curves of relative electron transport rates (rETR) vs. irradiance of Gracilaria lemaneiformis under different nutrients treatments

光合参数	LNLP	LNHP	HNLP	HNHP
最大电子传递速率 Maximum roelative electron transfer rate (rETR_max)	89.15±4.31^a	92.78±4.89^a	84.64±4.46^a	53.68±2.93^b
光能利用效率 Photosynthetic efficiency (α)	0.136±0.024^a	0.160±0.011^a	0.140±0.001^a	0.131±0.041^a

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氮磷水平对龙须菜生长和光合特性的影响

EFFECTS OF NITROGEN AND PHOSPHOROUS LEVELS ON GROWTH AND PHOTOSYNTHETIC TRAITS OF GRACILARIA LEMANEIFORMIS (RHODOPHYTA)

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