大别山山核桃种群遗传多样性研究

doi:10.17521/cjpe.2006.0070

摘要/Abstract

摘要：

为了更有效地保护和合理开发大别山山核桃(Carya dabieshanensis)资源,该文利用RAPD分子标记技术,对3个天然大别山山核桃种群的90个单株的遗传多样性、种群内和种群间的遗传变异进行了研究,结果表明:20对10 bp随机引物共检测到238条谱带,其中多态带为162条,占68.1%。遗传多样性分析结果显示: Shannon多样性指数为0.476 1,58.18%的变异分布于群体内,而种群间变异占了41.82%;Nei指数群体总基因多样度为0.314 5,群体内平均基因多样度(H_S)为0.186 5,群体间的基因多样度(H_ST)为0.128 0,群体Nei基因分化系数(G_ST)为0.406 7,说明40.67%的变异存在于种群间,群体内的变异占了总变异的59.33%,与Shannon多样性指数相比基本一致,均表明种群内有较丰富的遗传变异,这为优良品种选育提供广阔前景;种群间的基因流(N_m)为0.730 6,证明种群间遗传交换较小,这与环境适应性和高山阻隔有一定的关系。

关键词: 山核桃, 种群, RAPD分析, 遗传多样性

Abstract:

The genetic diversity and genetic variation within and among three natural Carya dabieshanensis populations were studied using RAPD analysis. The result showed that 238 loci were detected by using 20 random primers (10 bp) of which 162 loci were polymorphic (68.1%). Species genetic diversity indicated by Shannon's index was 0.476 1, 58.18% of which was due to within population genetic diversity and 41.82% among population variation. Species gene diversity indicated by Nei's index was 0.314 5, gene diversity within populations (H_S) was 0.186 5, gene diversity among populations (H_ST) was 0.128 0 and the coefficient of gene differentiation (G_ST) among the populations was 0.406 7, 59.33% of which was within populations and 40.67% was among populations. The gene diversity estimated by Nei's index was consistent with that estimated by Shannon's index. These results suggest that there is rich genetic within population variation, which offers excellent prospects for seeding selection. The estimate of gene flow from G_ST (N_m) was 0.730 6, indicating that genetic recombination between populations is quite small, which probably is related to environmental adaptations of C. debieshanensis and population isolation in their high mountain habitat.

Key words: Carya dabieshanensis, Population, RAPD analysis, Genetic diversity

王正加, 黄有军, 郭传友, 黄坚钦, 王华芳. 大别山山核桃种群遗传多样性研究. 植物生态学报, 2006, 30(3): 534-538. DOI: 10.17521/cjpe.2006.0070

WANG Zheng-Jia, HUANG You-Jun, GUO Chuan-You, HUANG Jian-Qin, WANG Hua-Fang. RAPD ANALYSIS ON GENETIC DIVERSITY OF CARYA DABIESHANENSIS POPULATIONS. Chinese Journal of Plant Ecology, 2006, 30(3): 534-538. DOI: 10.17521/cjpe.2006.0070

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2006.0070

https://www.plant-ecology.com/CN/Y2006/V30/I3/534

图/表 5

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种群 Population	地理位置 Geographical location	海拔 Elevation (m)
西园 Xiyuan	31°10' N, 115°37' E	530
仙桃 Xiantao	31°41' N, 115°40' E	750
平田 Pingtian	30°37' N, 114°54' E	640

种群 Population	地理位置 Geographical location	海拔 Elevation (m)
西园 Xiyuan	31°10' N, 115°37' E	530
仙桃 Xiantao	31°41' N, 115°40' E	750
平田 Pingtian	30°37' N, 114°54' E	640

种群 Population	样本数 Number of samples	位点数 Number of loci	多态位点数 Number of polymorphic loci	多态位点百分率 Percentage of polymorphic loci
西园 Xiyuan	30	238	129	54.0
平田 Pingtian	30	238	131	55.2
仙桃 Xiantao	30	238	127	53.2
总计Total	240	238	162	68.1

种群 Population	样本数 Number of samples	位点数 Number of loci	多态位点数 Number of polymorphic loci	多态位点百分率 Percentage of polymorphic loci
西园 Xiyuan	30	238	129	54.0
平田 Pingtian	30	238	131	55.2
仙桃 Xiantao	30	238	127	53.2
总计Total	240	238	162	68.1

	西园 Xiyuan	平田 Pingtian	仙桃 Xiantao	种的基因多样性 Species genetic diversity	种群内基因多样性 Within-population genetic diversity (H_s)	种群间基因多样性 Inter-population genetic diversity (H_ST)	种群间基因分化系数 Coefficient of gene differentiation (G_ST)
Shonnon多样性指数 Shonnon Index (I)	0.279 8	0.280 1	0.265 1	0.476 1	0.275 0	0.201 1	0.418 2
Nei指数 Nei index	0.189 0	0.191 6	0.178 9	0.314 5	0.186 5	0.128 0	0.406 3