不同浓度NaCl和Na2CO3处理对菊芋幼苗光合及叶绿素荧光的影响

doi:10.3773/j.issn.1005-264x.2008.01.018

摘要/Abstract

摘要：

以砂培菊芋(Helianthus tuberosus)幼苗作为试验材料,分别进行不同浓度NaCl (50、100、150、200、250 mmol·L^-1)和Na₂CO₃ (25、50、75、100、125 mmol·L^-1)胁迫处理,以1/2全营养液作为对照,处理7 d后研究NaCl和Na₂CO₃胁迫处理对菊芋幼苗叶片光合作用及叶绿素动力学参数的影响。结果表明:1)在NaCl处理下,当浓度小于150 mmol·L^-1时,增加了菊芋的叶绿素含量、净光合速率(Net photosynthetic rate, P_n)和气孔导度(Stomatal conductivity, G_s),对荧光参数PSⅡ的电子传递情况(F_m/F_o)、PSⅡ原初光能转换效率(F_v/F_m)、PSⅡ量子效率(Actual quantum yield of PSⅡ under actinic irradiation, Φ_PSⅡ)和光化学猝灭系数(Photochemical quenching coefficient, q_P)和非光化学猝灭系数(Non-photochemical quenching coefficient, NPQ)没有显著影响,随着浓度的增加,各项生理指标与对照相比除了NPQ显著增加,其余均显著降低;2)在Na₂CO₃胁迫处理下,随着Na₂CO₃浓度的增加,与对照相比菊芋幼苗叶绿素含量、P_n、G_s以及叶绿素a荧光诱导动力学参数F_m/F_o、F_v/F_m、Φ_PSⅡ和q_P均显著降低,NPQ显著增加;3)就NaCl和Na₂CO₃相比而言,在相同Na ⁺浓度情况下,处于Na₂CO₃胁迫下的菊芋幼苗的叶绿素含量、P_n、G_s以及叶绿素a荧光诱导动力学参数F_m/F_o、F_v/F_m、Φ_PSⅡ和q_P下降幅度和NPQ的增加幅度均显著大于NaCl,这说明NaCl和Na₂CO₃胁迫均对菊芋幼苗造成不同程度的伤害,但在相同Na⁺浓度情况下,Na₂CO₃的伤害程度大于NaCl。由此说明菊芋对盐的忍耐程度高于碱。

关键词: NaCl, Na₂CO₃, 菊芋, 胁迫, 光合作用, 荧光

Abstract:

Aims Our objective was to explore the effects of saline-alkali stress on photosynthetic apparatus of Helianthus tuberosus seedlings.
Methods H. tuberosus seedlings were treated with NaCl (50, 100, 150, 200, 250 mmol·L^-1) and Na₂CO₃ (25, 50, 75, 100, 125 mmol·L^-1) in plastic pots with sand. Others were cultured in modulated half-strength Hoagland solution as a control. We studied the effects of NaCl and Na₂CO₃ on net photosynthetic rate (P_n) and parameters of chlorophyll fluorescence by measuring chlorophyll content, P_n, stomatal conductivity (G_s) and fluorescence parameters after 7 days.
Important findings Significant increases of chlorophyll content, P_n and G_s compared with the control were observed under the 150 mmol·L^-1 NaCl treatment, whereas there were no effects on fluorescence parameters, F_v/F_m (maximal photochemical efficiency of photosystem Ⅱ (PSⅡ) while all PSⅡ reaction centers are open), F_m/F_o (another expression for the maximal photochemical efficiency of PSⅡ), Φ_PSⅡ (actual quantum yield of PSⅡ under actinic irradiation), q_P (photochemical quenching coefficient), and NPQ (non-photochemical quenching coefficient). But with greater NaCl concentration, the chlorophyll content, P_n, G_s and fluorescence parameters significantly decreased except for an increase of NPQ compared with the control. When H. tuberosus seedlings were treated with Na₂CO₃, chlorophyll content, P_n, G_s, F_v/F_m, F_m/F_o, Φ_PSⅡ and q_P significantly decreased and NPQ increased compared with the control. At the same concentration of Na ⁺, the decrease of chlorophyll content, P_n, G_s, F_v/F_m, F_m/F_o, Φ_PSⅡ, q_P and the increase of NPQ were higher in Na₂CO₃ stress than in NaCl stress. All results suggested that the tolerance of H. tuberosus seedlings to saline stress was higher than to alkaline stress.

Key words: NaCl, Na₂CO₃, Helianthus tuberosus, stress, photosynthesis, fluorescence

薛延丰, 刘兆普. 不同浓度NaCl和Na₂CO₃处理对菊芋幼苗光合及叶绿素荧光的影响. 植物生态学报, 2008, 32(1): 161-167. DOI: 10.3773/j.issn.1005-264x.2008.01.018

XUE Yan-Feng, LIU Zhao-Pu. EFFECTS OF NaCl AND Na₂CO₃ STRESSES ON PHOTOSYNTHESIS AND PARAMETERS OF CHLOROPHYLL FLUORESCENCE IN HELIANTHUS TUBEROSUS SEEDLINGS. Chinese Journal of Plant Ecology, 2008, 32(1): 161-167. DOI: 10.3773/j.issn.1005-264x.2008.01.018

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https://www.plant-ecology.com/CN/Y2008/V32/I1/161

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参考文献 29

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		Na⁺浓度 Na⁺ concentration (mmol·L^-1)
		0	50	100	150	200	250
F_m/F_o	NaCl	3.443±0.142	3.321±0.181	3.013±0.165	2.635±0.121	2.111±0.144	1.744±0.199
	Na₂CO₃	3.443±0.142	2.997±0.128	2.241±0.131	1.757±0.159	1.486±0.154	1.189±0.146
F_v/F_m	NaCl	0.737±0.037	0.726±0.025	0.714±0.038	0.648±0.039	0.552±0.033	0.461±0.024
	Na₂CO₃	0.737±0.037	0.696±0.024	0.579±0.035	0.517±0.034	0.437±0.025	0.308±0.032
Φ_PSⅡ	NaCl	0.166±0.009	0.160±0.009	0.151±0.011	0.096±0.008	0.080±0.006	0.063±0.008
	Na₂CO₃	0.166±0.009	0.143±0.006	0.107±0.011	0.080±0.006	0.062±0.009	0.037±0.006
q_P	NaCl	0.316±0.013	0.310±0.015	0.291±0.018	0.259±0.013	0.219±0.014	0.192±0.019
	Na₂CO₃	0.316±0.013	0.286±0.019	0.226±0.014	0.191±0.015	0.160±0.016	0.147±0.011
NPQ	NaCl	0.513±0.034	0.522±0.036	0.535±0.037	0.612±0.034	0.683±0.039	0.736±0.035
	Na₂CO₃	0.513±0.034	0.596±0.039	0.655±0.032	0.718±0.038	0.766±0.038	0.817±0.034

		Na⁺浓度 Na⁺ concentration (mmol·L^-1)
		0	50	100	150	200	250
F_m/F_o	NaCl	3.443±0.142	3.321±0.181	3.013±0.165	2.635±0.121	2.111±0.144	1.744±0.199
	Na₂CO₃	3.443±0.142	2.997±0.128	2.241±0.131	1.757±0.159	1.486±0.154	1.189±0.146
F_v/F_m	NaCl	0.737±0.037	0.726±0.025	0.714±0.038	0.648±0.039	0.552±0.033	0.461±0.024
	Na₂CO₃	0.737±0.037	0.696±0.024	0.579±0.035	0.517±0.034	0.437±0.025	0.308±0.032
Φ_PSⅡ	NaCl	0.166±0.009	0.160±0.009	0.151±0.011	0.096±0.008	0.080±0.006	0.063±0.008
	Na₂CO₃	0.166±0.009	0.143±0.006	0.107±0.011	0.080±0.006	0.062±0.009	0.037±0.006
q_P	NaCl	0.316±0.013	0.310±0.015	0.291±0.018	0.259±0.013	0.219±0.014	0.192±0.019
	Na₂CO₃	0.316±0.013	0.286±0.019	0.226±0.014	0.191±0.015	0.160±0.016	0.147±0.011
NPQ	NaCl	0.513±0.034	0.522±0.036	0.535±0.037	0.612±0.034	0.683±0.039	0.736±0.035
	Na₂CO₃	0.513±0.034	0.596±0.039	0.655±0.032	0.718±0.038	0.766±0.038	0.817±0.034

不同浓度NaCl和Na₂CO₃处理对菊芋幼苗光合及叶绿素荧光的影响

EFFECTS OF NaCl AND Na₂CO₃ STRESSES ON PHOTOSYNTHESIS AND PARAMETERS OF CHLOROPHYLL FLUORESCENCE IN HELIANTHUS TUBEROSUS SEEDLINGS

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