植物生态学报 ›› 2001, Vol. 25 ›› Issue (4): 483-487.

• 简报 • 上一篇    下一篇

去劣疏伐对油松种子园交配系统及遗传多样性影响的研究

张冬梅,李悦,沈熙环,周世良,张春晓   

  • 出版日期:2001-04-10 发布日期:2001-04-10
  • 通讯作者: 张冬梅

Mating System and Genetic Diversity in a Pinus tabulaeformis Carr Seed Orchard Before and AfterThinning

ZHANG Dong-Mei, LI Yue, SHEN Xi-Huan, ZHOU Shi-Liang and ZHANG Chun-Xiao   

  • Online:2001-04-10 Published:2001-04-10
  • Contact: XU Cheng-Yang

摘要: 为了解去劣疏伐对种子园群体交配系统和遗传组成的影响程度,采用水平淀粉凝胶电泳方法,对10个等位酶位点上,分别用混合交配模型(MLT)和Biosys-Ⅰ程序,分析了去劣疏伐前后辽宁兴城油松种子园3个年份群体的交配系统和遗传多样性变化。种子园去劣疏伐前(1993年)种子试样多位点异交率为0.962,而疏伐后2个年份(1996年和2000年)试样的多位点异交率明显减小,分别为0.795和0.801。疏伐后种子园的自交率有升高的趋势,从疏伐前的0.038到疏伐后的0.205和0.199。近交系数也有明显变化,疏伐前为0.141,疏伐后分别为0.341和0.239。去劣疏伐后种子园的每个位点的平均等位基因数(A)和多态位点百分率(P)都有不同程度的降低。本文对影响种子园交配系统和遗传多样性的因素作了探讨。

关键词: 油松, 种子园, 去劣疏伐, 交配系统, 遗传多

Abstract: Variation in mating system and genetic diversity of seed samples collected 3 years before and 3 years after thinning were examined in a clonal seed orchard of Pinus tabulaeformis Carr. in Liaoning province. Allozyme markers with 10 loci were used in the study of genetic diversity. The multilocus outcrossing, selfing and inbreeding rates of the seed orchard before thinning in 1993 were 0.962, 0.038 and 0.141 respectively. The multilocus outcrossing rate in the thinned seed orchard in 1996 and 2000 decreased to 0.795 and 0.801 respectively. At the same time the multilocus selfing rate increased to 0.205 and 0.199 and the genetic diversity indices A and P decreased to different degrees after thinning. The results showed that thinning based on phenotype had a strong influence on mating system but a lesser effect in genetic diversity indices. Factors influencing the mating system and genetic diversity are also discussed.

Key words: Pinus tabulaeformis, Seed orchard thinning, Mating system, Genetic diversity