植物生态学报 ›› 2012, Vol. 36 ›› Issue (9): 973-981.DOI: 10.3724/SP.J.1258.2012.00973
收稿日期:
2011-10-10
接受日期:
2012-05-07
出版日期:
2012-10-10
发布日期:
2012-09-06
通讯作者:
陈保冬
作者简介:
(E-mail: bdchen@rcees.ac.cn)Received:
2011-10-10
Accepted:
2012-05-07
Online:
2012-10-10
Published:
2012-09-06
Contact:
CHEN Bao-Dong
摘要:
在模拟干旱条件下, 研究了接种丛枝菌根(AM)真菌Glomus intraradices对玉米(Zea mays)根部13种质膜水孔蛋白基因表达的影响, 同时观测了AM真菌自身水孔蛋白基因的表达情况。结果表明, 干旱条件下, 除Zm PIP1;3、Zm PIP1;4、Zm PIP1;5和Zm PIP2;2之外的接种处理能显著提高根部其他8种质膜水孔蛋白基因的表达(Zm PIP2;7表达量未检测出), 并且AM真菌菌丝中水孔蛋白基因GintAQP1表达也显著增强。与此同时, 接种处理明显改善了植物水分状况, 提高了叶片水势。AM真菌增强宿主植物根部及自身的水孔蛋白基因的表达对于提高植物抗旱性具有潜在的重要贡献。
李涛, 陈保冬. 丛枝菌根真菌通过上调根系及自身水孔蛋白基因表达提高玉米抗旱性. 植物生态学报, 2012, 36(9): 973-981. DOI: 10.3724/SP.J.1258.2012.00973
LI Tao, CHEN Bao-Dong. Arbuscular mycorrhizal fungi improving drought tolerance of maize plants by up-regulation of aquaporin gene expressions in roots and the fungi themselves. Chinese Journal of Plant Ecology, 2012, 36(9): 973-981. DOI: 10.3724/SP.J.1258.2012.00973
接种处理 Inoculation treatment | 菌根侵染率 Mycorrhizal colonization rate (%) | |
---|---|---|
正常水分 Well-watered | 干旱 Drought | |
不接种 Non-inoculated | 0.0 | 0.0 |
接种 Inoculated | 35.1* | 38.5* # |
表1 不同水分条件下玉米根系菌根侵染率(平均值±标准偏差, n = 4)
Table 1 Mycorrhizal colonization rate of maize roots under different water regimes (mean ± SD, n = 4)
接种处理 Inoculation treatment | 菌根侵染率 Mycorrhizal colonization rate (%) | |
---|---|---|
正常水分 Well-watered | 干旱 Drought | |
不接种 Non-inoculated | 0.0 | 0.0 |
接种 Inoculated | 35.1* | 38.5* # |
图1 不同水分条件下AM真菌Glomus intraradices对玉米植株干重的影响(平均值±标准偏差, n = 4)。分别对地上部和根部进行双因素方差分析和t检验。*表示相同水分处理条件下不同接种处理间在5%水平上差异显著。
Fig. 1 Effects of inoculation with arbuscular mycorrhizal (AM) fungus Glomus intraradices on maize dry weights under different water regimes (mean ± SD, n = 4). Two-way ANOVA and t-test were performed for shoots and roots, respectively. * indicates significant difference (p < 0.05) between inoculation treatments under the same water treatment.
图2 不同水分条件下AM真菌Glomus intraradices对玉米植株磷浓度的影响(平均值±标准偏差, n = 4)。分别对地上部和根部进行双因素方差分析和t检验。*表示相同水分处理条件下不同接种处理间在5%水平上差异显著。
Fig. 2 Effects of inoculation with arbuscular mycorrhizal (AM) fungus Glomus intraradices on P concentration of maize under different water regimes (mean ± SD, n = 4). Two-way ANOVA and t-test were performed for shoots and roots, respectively. * indicates significant difference (p < 0.05) between inoculation treatments under the same water treatment.
地上部干重 Shoot dry weight | 根部干重 Root dry weight | 地上部磷含量 Shoot P concentration | 根部磷含量 Root P concentration | 叶片水势 Leaf water potential | |
---|---|---|---|---|---|
接种处理 Inoculation treatment (I) | * | ns | * | ns | * |
水分处理 Water treatment (W) | ns | ns | ns | ns | ** |
交互作用 I × W interaction | ns | ns | ns | ns | ns |
表2 地上部和根部干重、磷浓度及叶片水势的双因素(接种处理和水分处理)方差分析
Table 2 Two-way ANOVA of shoot and root dry weight, P concentration and leaf water potential as influenced by inoculation and water treatments
地上部干重 Shoot dry weight | 根部干重 Root dry weight | 地上部磷含量 Shoot P concentration | 根部磷含量 Root P concentration | 叶片水势 Leaf water potential | |
---|---|---|---|---|---|
接种处理 Inoculation treatment (I) | * | ns | * | ns | * |
水分处理 Water treatment (W) | ns | ns | ns | ns | ** |
交互作用 I × W interaction | ns | ns | ns | ns | ns |
图3 不同水分条件下AM真菌Glomus intraradices对玉米叶片水势的影响(平均值±标准偏差, n = 4)。#表示相同接种处理下不同水分处理间在5%水平上差异显著。
Fig. 3 Effects of inoculation with arbuscular mycorrhizal (AM) fungus Glomus intraradices on leaf water potential of maize under different water regimes (mean ± SD, n = 4). # indicates significant difference (p < 0.05) between water treatments under the same inoculation treatment.
图4 不同水分条件下AM真菌Glomus intraradices对玉米根部质膜水孔蛋白基因表达的影响(平均值±标准偏差, n = 4)。#表示在相同接种处理下不同水分处理间在5%水平差异显著。*表示在相同水分处理条件下不同接种处理间在5%水平上差异显著。对不同基因分别进行统计分析。D, 干旱处理; W, 正常水分。
Fig. 4 Effects of arbuscular mycorrhizal (AM) fungus Glomus intraradices on expression of plasma membrane aquaporin gene in maize roots under different water regimes (mean ± SD, n = 4). # indicates significant difference (p < 0.05) between water treatments under the same inoculation treatment. * indicates significant difference (p < 0.05) between inoculation treatments under the same water treatment. Statistical analysis was performed for each aquaporin gene respectively. D, drought treatment; W, well-watered treatment.
Zm PIP1;1 | Zm PIP1;2 | Zm PIP1;3 | Zm PIP1;4 | Zm PIP1;5 | Zm PIP1;6 | Zm PIP2;1 | Zm PIP2;2 | Zm PIP2;3 | Zm PIP2;4 | Zm PIP2;5 | Zm PIP2;6 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
接种处理 Inoculation treatment (I) | * | ** | ns | ns | ns | ** | ** | ** | ** | * | ** | ** |
水分处理 Water treatment (W) | ** | ** | ns | ns | * | ns | * | ns | ns | ns | ** | ns |
交互作用 I ×W interaction | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns |
表3 12个质膜水孔蛋白基因表达的双因素(接种处理和水分处理)方差分析
Table 3 Two-way ANOVA of gene expression of 12 Zm PIPs as influenced by inoculation and water treatments.
Zm PIP1;1 | Zm PIP1;2 | Zm PIP1;3 | Zm PIP1;4 | Zm PIP1;5 | Zm PIP1;6 | Zm PIP2;1 | Zm PIP2;2 | Zm PIP2;3 | Zm PIP2;4 | Zm PIP2;5 | Zm PIP2;6 | |
---|---|---|---|---|---|---|---|---|---|---|---|---|
接种处理 Inoculation treatment (I) | * | ** | ns | ns | ns | ** | ** | ** | ** | * | ** | ** |
水分处理 Water treatment (W) | ** | ** | ns | ns | * | ns | * | ns | ns | ns | ** | ns |
交互作用 I ×W interaction | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns | ns |
图5 干旱胁迫对AM真菌Glomus intraradices水孔蛋白基因表达的影响(平均值±标准偏差, n = 4)。#表示在相同接种处理下不同水分处理间在5%水平差异显著。
Fig. 5 Effects of drought stress on expression of arbuscular mycorrhizal (AM) fungus Glomus intraradices aquaporin gene (mean ± SD, n = 4). # indicates significant difference (p < 0.05) between water treatments under the same inoculation treatment.
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