植物生态学报 ›› 2017, Vol. 41 ›› Issue (3): 311-324.DOI: 10.17521/cjpe.2016.0267

所属专题: 生态化学计量

• 研究论文 • 上一篇    下一篇

云南元谋不同海拔土壤微生物对车桑子碳、氮、磷化学计量特征及土壤特性的影响

王雪梅1,2, 闫帮国1,2,3, 赵广1,2, 史亮涛3, 刘刚才1, 方海东3,*()   

  1. 1中国科学院水利部成都山地灾害与环境研究所, 中国科学院山地表生过程与生态调控重点实验室, 成都 610041
    2中国科学院大学, 北京 100049
    3云南省农业科学院热区生态农业研究所, 云南元谋 651300
  • 出版日期:2017-03-10 发布日期:2017-04-12
  • 通讯作者: 方海东
  • 作者简介:* 通信作者Author for correspondence (E-mail:sunzhiqiang1956@sina.com)
  • 基金资助:
    国家自然科学基金(41471232和31460127)

Effects of microorganism on carbon, nitrogen and phosphorus of Dodonaea viscosa and the soils from different elevations in Yuanmou, Yunnan, China

Xue-Mei WANG1,2, Bang-Guo YAN1,2,3, Guang ZHAO1,2, Liang-Tao SHI3, Gang-Cai LIU1, Hai-Dong FANG3,*()   

  1. 1Key Laboratory of Mountain Surface Processes and Ecological Regulation, Chinese Academy of Sciences, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences and Ministry of Water Conservancy, Chengdu 610041, China

    2University of Chinese Academy of Sciences, Beijing 100049, China
    and
    3Institute of Tropical Eco-Agriculture, Yunnan Academy of Agricultural Sciences, Yuanmou, Yunnan 651300, China
  • Online:2017-03-10 Published:2017-04-12
  • Contact: Hai-Dong FANG
  • About author:KANG Jing-yao(1991-), E-mail: kangjingyao_nj@163.com

摘要:

了解不同气候下土壤微生物对植物生态化学计量特征的影响有利于理解未来温度变化下植物与土壤的相互关系。该文以车桑子(Dodonaea viscosa)为研究对象, 从云南省元谋县两个海拔区采集车桑子根际土壤样品, 并设定相应的两个温度处理, 通过微生物灭菌和接种处理, 测定了车桑子叶片养分及其所在的土壤特性, 研究了温度、微生物及温度与微生物的交互作用对车桑子的碳(C)、氮(N)、磷(P)化学计量特征的影响, 分析其N、P养分特征与土壤特性间的关系。研究结果表明: 相比于灭菌处理, 两个海拔区的土壤微生物对车桑子养分吸收都有促进作用, 对P的促进作用极为显著。温度对车桑子C、N、P化学计量特征无显著影响, 但温度与微生物的交互作用对车桑子的C、N、P化学计量特征有显著影响。这种交互作用主要体现在: 在接种高海拔区土壤微生物的处理中, 温度没有显著影响车桑子的C、N、P化学计量特征, 而在接种低海拔区土壤微生物的处理中, 相比于高温条件, 低温显著抑制了车桑子对N、P的养分吸收。土壤微生物对养分吸收的促进作用可能源于其中有益微生物(如丛枝菌根真菌)的直接促进作用, 而并非是通过土壤微生物的养分循环过程。由于接种微生物处理使车桑子从土壤中吸收了更多的N、P, 从而使该处理的土壤有效N、P显著低于灭菌处理。在元谋干热河谷未来气温变凉的情况下, 由于土壤微生物对植物的反馈作用, 该区车桑子的生长可能会受到一定的抑制。

关键词: 生态化学计量, 土壤微生物, 植物-土壤反馈, 气候变化, 车桑子

Abstract:

Aims Understanding the effects of soil microorganism at different elevations on plant C:N:P stoichiometry can help us to understand the plant-soil interactions in the context of climate change. Our aim was to quantify the independent and interactive effects of soil microbial communities and temperatures on the C, N, and P in the leaves of Dodonaea viscosa—a global widespread species. Methods Rhizosphere soils of D. viscosa were collected from two elevation zones in Yuanmou County, Yunnan Province. A 2 × 3 factorial experiment with six replications was conducted using climate chambers. The leaf C, N and P contents and the soil properties were measured after three months of the treatments. Important findings Compared with the autoclaved treatment, inoculated rhizosphere soils from both high and low elevations had higher nutrient absorption, especially P uptake. Temperature produced no significant effect on leaf C:N:P stoichiometry, but the interactive effect of temperature and microbial treatment appeared significant. For inoculated rhizosphere soils from high elevation, temperature had no significant effect on leaf C:N:P stoichiometry. For inoculated rhizosphere soils from low elevation, leaf N and P contents under low temperature were significantly lower than those with warmer soils. The promoting effect of soil microorganisms on nutrient uptake may be due to the direct effect of beneficial microorganisms (e.g., mycorrhizal fungi), but not through the alteration of nutrient cycling process. Because D. viscosa in the inoculated rhizosphere soils absorbed more N and P from the soil than those in autoclaved soil, the available N and P in inoculated rhizosphere soils were lower than those in autoclaved soils. As predicted future temperature will be lower in the studied region, the growth of D. viscosa may be negatively affected through plant-microbe feedbacks.

Key words: ecological stoichiometry, soil microorganisms, plant-soil feedback, climate change, Dodonaea viscosa