POPULATION GENETIC DIFFERENTIATIONS IN THE INVASIVE PLANT MIKANIA MICRANTHA IN CHINA

doi:10.17521/cjpe.2007.0088

Abstract

Abstract:

Aims Genetic changes after introduction may contribute to the success of invasive plants. Our objective was to measure genetic diversity and genetic differentiation in the invasive Mikania micrantha in order to assess its invasive prospects.
Methods Inter-simple sequence repeat (ISSR) markers were used to analyze genetic diversity and genetic differentiation in eight populations of M. micrantha.
Important findings The 12 ISSR primers used in this study produced 171 loci, of which 103 were polymorphic. At the species level, the percentage of polymorphic loci (P) was 60.23%, Shannon's information index (I) 0.281 8 and Nei's gene diversity (h) 0.184 9, indicating high genetic diversity in M. micrantha. Analysis of molecular variance (AMOVA) revealed that a high proportion (36.49%) of the total genetic variation was partitioned among populations, suggesting significant genetic differentiations among the eight populations of M. micrantha. Gene differentiation coefficient (G_ST) among populations was 0.352 4, and gene flow among populations was 0.918 7. Average genetic similarity of the eight populations was 0.915 5, while average genetic distance was 0.088 4. Using unweighted pair group method arithmetic average (UPGMA), the eight populations were clustered into two groups: the two populations on Neilingding Island were one group and the other six from mainland China were the other.

Key words: Mikania micrantha, invasive plant, genetic diversity, genetic differentiation, ISSR

LI Jun-Min, DONG Ming, ZHONG Zhang-Cheng. POPULATION GENETIC DIFFERENTIATIONS IN THE INVASIVE PLANT MIKANIA MICRANTHA IN CHINA[J]. Chin J Plant Ecol, 2007, 31(4): 680-688.

Add to citation manager EndNote|Ris|BibTeX

URL: https://www.plant-ecology.com/EN/10.17521/cjpe.2007.0088

https://www.plant-ecology.com/EN/Y2007/V31/I4/680

Figures/Tables 8

References 57

[1]	Aguinagalde I, Hampe A, Mohanty A, Martín JP, Duminil J, Petit RJ (2005). Effects of life-history traits and species distribution on genetic structure at maternally inherited markers in European trees and shrubs. Journal of Biogeography, 32, 329-339. DOI URL
[2]	Ammiraju JSS, Dholakin BB, Sntra DK, Singh H, Lagu MD, Tamhankar SA, Dhaiual HS, Rao VS, Gupta VS, Ranjekar PK (2001). Identification of inter simple sequence repeat (ISSR)markers associated with seed size in wheat. Theoretical Application of Genetics, 102, 726-732.
[3]	Bakir M, Facey PC, Hassan I, Mulder WH, Porter RB (2004). Mikanolide from Jamaican Mikania micrantha. Acta Crystallographica, Section C, Crystal Structure Communications, 60, 798-800.
[4]	Brown AHD, Marshall DR (1981). Evolutionary changes accompanying colonization in plants. In: Scudder GCE, Reveal JL eds. Colonization, Succession and Stability. Blackwell Scientific Publications, Oxford,
[5]	Esselman EJ, Jianqiang L, Crawford DJ, Windus JL, Wolfe AD (1999). Clonal diversity in the rare Calamagrostis porteri spp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and intersimple sequence repeat (ISSR) markers. Molecular Ecology, 8, 443-451. DOI URL
[6]	Excoffier L, Smouse PF, Quattro JM (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics, 131, 479-491. URL PMID
[7]	Feng HL( 冯惠玲), Cao HL(曹洪麟), Liang XD(梁晓东), Zhou X(周霞), Ye WH(叶万辉) (2002). The distribution and harmful effect of Mikania micrantha in Guangdong. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 10, 263-270. (in Chinese with English abstract)
[8]	Godt MJW, Hamrick JL(1991). Genetic variation in Lathyrus latifolius (Leguminosae). American Journal of Botany, 78, 1163-1171. DOI URL
[9]	Govindaraju DR (1988). Relationship between dispersal ability and levels of gene flow in plants. Oikos, 52, 31-35. DOI URL
[10]	Hamrick JL, Godt MJW (1989). Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS eds. Plant Population Genetics, Breeding, and Genetic Resources. Sinauer, Sunderland, USA,
[11]	Hamrick JL, Godt MJW (1990). Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS eds. Plant Population Genetics, Breeding, and Genetic Resources. Sinauer, Sunderland, USA.
[12]	Hamrick JL, Godt MJW, Sherman-Broyles SL (1992). Factors influencing levels of genetic diversity in woody plant species. New Forests, 6, 95-124. DOI URL
[13]	Hamrick JL, Linhart YB, Mitton JB (1979). Relationships between life history characteristics and electrophoretically detectable genetic variation in plants. Annual Review of Ecological Systematics, 10, 173-200. DOI URL
[14]	Hollingsworth ML, Hollingsworth PM, Jenkins GI, Bailey JP, Ferris C (1998). The use of molecular markers to study patterns of genotypic diversity in some invasive alien Fallopia spp. (Polygonaceae). Molecular Ecology, 7, 1681-1691. DOI URL
[15]	Hu YJ (胡玉佳), Paul PHB (毕培曦) (1994). A study on life cycle and response to herbicides of Mikania micrantha. Acta Scientiarum Naturalium Universitatis Sunyatseni (中山大学学报(自然科学版)) , 33(4) , 88-95. (in Chinese with English abstract)
[16]	Ismail BS, Chong TV (2002). Effects of aqueous extracts and decomposition of Mikania micrantha H.B.K. debris on selected agronomic crops. Weed Biology and Management, 2, 31-38. DOI URL
[17]	Kong GH(孔国辉), Wu QG(吴七根), Hu QM(胡启明), Ye WH(叶万辉) (2000a). Further supplementary data on Mikania micrantha H.B.K. (Asteraceae). Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 8(2), 128-130. (in Chinese with English abstract)
[18]	Kong GH(孔国辉), Wu QG(吴七根), Hu QM(胡启明) (2000b). Emergence of invasive weed Mikania micrantha H.B.K. in our county. Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 8(1), 27. (in Chinese)
[19]	Li JM(李钧敏), Ke SS(柯世省), Jin ZX(金则新) (2002). Extraction and determination of DNA from Heptacodium miconioides. Guihaia (广西植物), 22, 499-502. (in Chinese with English abstract)
[20]	Li MG(李鸣光), Zhang WY(张炜银), Wang BS(王伯荪), Zhang JL(张军丽), Zan QJ(昝启杰), Wang YJ(王勇军) (2002). A preliminary study on the seed germination in Mikania micrantha. Acta Scientiarum Naturalium Universitis Sunyatseni (中山大学学报(自然科学版)), 41(6), 57-59. (in Chinese with English abstract)
[21]	Li WG, Shen JJ, Wang JB (2005). Genetic diversity of the annual weed Monochoria vaginalis in southern China detected by random amplified polymorphic DNA and inter-simple sequence repeat analyses. Weed Research, 45, 424-430. DOI URL
[22]	Li A, Ge S (2001). Genetic variation and colonal diversity of Psammochloa villosa (Poaceae) detected by ISSR markers. Annual of Botany, 87, 585-590.
[23]	Ma CL(马翠兰), Liu XS(刘星释), Zhang YL(张玉兰) (2001). Application of DNA molecular markers to fruit trees. Journal of Inner Mongolia Agriculature University (Natural Science Edition) (内蒙古农业大学报(自然科学版)), 22(2), 105-112. (in Chinese with English abstract)
[24]	Meekins JF, Ballard HE, McCarthy BC (2001). Genetic variation and molecular biogeography of a North American invasive plant species ( Alliaria Petiolata, Brassicaceae). International Journal of Plant Science, 162, 161-169. DOI URL
[25]	Mooney HA, Cleland EE (2001). The evolutionary impact of invasive species. Proceedings of the National Academy Sciences of the United States of America, 98, 5446-5451. DOI URL
[26]	Nei M (1972). Genetic distance between populations. American Naturalist, 106, 283-292. DOI URL
[27]	Nei M, Maruyama T, Chakraborty R (1975). The bottleneck effect and genetic variability in populations. Evolution, 29, 1-10. DOI URL PMID
[28]	Nicollier G, Thompson AC (1981). Essential oil and terpenoids of Mikania micrantha. Phytochemistry, 20, 2587-2588. DOI URL
[29]	Nissen SJ, Masters RA, Lee DJ, Rowe ML(1995). DNA-based marker systems to determine genetic diversity of weedy species and their application to biocontrol. Weed Science, 43, 504-513. DOI URL
[30]	Novak SJ, Mack RN (1993). Genetic variation in Bromus tectorum (Poaceae): comparison between native and introduced populations. Heredity, 71, 167-176. DOI URL
[31]	Pappert RA, Hamrick JL, Donovan LA (2000). Genetic variation in Pueraria lobata (Fabaceae), an introduced, clonal, invasive plant of the southeastern United States. American Journal of Botany, 87, 1240-1245. URL PMID
[32]	Ren MX, Zhang QG, Zhang DY (2005). Random amplified polymorphic DNA markers reveal low genetic variation and a single dominant genotype in Eichhornia crassipes populations throughout China. Weed Research, 45, 236-244. DOI URL
[33]	Sakai AK, Allendorf FW, Holt JS, Lodge DM, Molofsky J, With KA, Baughman S, Cabin RJ, Cohen JE, Ellstrand NC, McCauley DE, O'Neil P, Parker IM, Thompson JN, Weller SG (2001). The population biology of invasive species. Annual Review of Ecology and Systematics, 32, 305-332. DOI URL
[34]	Schierenbeck KA, Hamrick JL, Mack RN (1995). Comparison of allozyme variability in a native and an introduced species of Lonicera. Heredity, 75, 1-9. DOI URL
[35]	Shao H(邵华), Peng SL(彭少麟), Liu YX(刘运笑), Zhang C(张弛), Xiang YC(向言词) (2002). The biological control and the natural enemy of Mikania micrantha H.B.K.'s in China. Ecologic Science (生态科学), 21, 33-36. (in Chinese with English abstract)
[36]	Shao WT(邵婉婷), Han SC(韩诗畴), Huang SS(黄寿山), Liu WH(刘文惠), Li KH(李开煌), Peng TX(彭统序), Li LY(李丽英) (2002). Progress on the control of invasive weed Mikania micrantha. Guangdong Agricultural Sciences (广东农业科学), 37(1), 43-45. (in Chinese)
[37]	Swamy PS, Ramakrishnan PS (1987a). Weed potential of Mikania micrantha H.B.K., and its control in fallows after shifting agriculture (Jhum) in North-East India. Agriculture, Ecosystems and Environment, 18, 195-204.
[38]	Swamy PS, Ramakrishnan PS (1987b). Contribution of Mikania micrantha during secondary succession following slash-and-burn agriculture (Jhum) in North-East India. I. Biomass litterfall and productivity. Forest Ecology and Management, 22, 229-237. DOI URL
[39]	Tsumura Y, Ohba K, Strauss SH (1996). Diversity and inheritance of inter-simple sequence repeat polymorphisms in Douglas fir (Pseudotsuga menzesii) and sugi (Cryptomeria japonica). Theoretical Application of Genetics, 92, 40-45.
[40]	Wain RP, Haller WT, Martin DF (1984). Genetic relationship among two forms of alligator weed. Journal of Aquatic Plant Management, 22, 104-105.
[41]	Wang BS(王伯荪), Liao WB(廖文波), Miao RH(缪汝槐) (2001). Revision of Mikania from China and the key of four relative species. Acta Scientiarum Naturalium Universitis Sunyatseni (中山大学学报(自然科学版)), 40(5), 72-75. (in Chinese with English abstract)
[42]	Wang BS(王伯荪), Wang YJ(王勇军), Liao WB(廖文波) (2004). Invasion Ecology and Control on Mikania micrantha (外来杂草薇甘菊的入侵生态及其治理). Science Press, Beijing. (in Chinese)
[43]	Wei XY, Huang HJ, Wu P, Cao HL, Ye WH (2004). Phenolic constituents from Mikania micrantha. Biochemical Systematics and Ecology, 32, 1091-1096. DOI URL
[44]	Wen DZ (温达志), Ye WH (叶万辉), Feng HL (冯惠玲), Cai CX (蔡楚雄) (2000). Comparison of basic photosynthetic characteristics between exotic invader weed Mikania micrantha and its companion species. Journal of Tropical and Subtropical Botany (热带亚热带植物学报) , 8, 139-146. (in Chinese with English abstract)
[45]	Wolfe AD, Xiang QY, Kephart SR (1998). Assessing hybridization in natural populations of Penstemon (Scrophulariaceae) using hypervariable intersimple sequence repeat (ISSR) bands. Molecular Ecology, 7, 1107-1125. DOI URL PMID
[46]	Wright S 1978. Evolution and the Genetics of Populations Vol 4. Variability Within and Among Natural Populations. University of Chicago Press, Chicago.
[47]	Yang QH(杨期和), Feng HL(冯惠玲), Ye WH (叶万辉), Cao HL(曹洪麟), Deng X(邓雄), Xu KY(许凯扬) (2003). An investigation of the effects of environmental factors on the flowering and seed setting of Mikania micrantha H.B.K. (Compositae). Journal of Tropical and Subtropical Botany (热带亚热带植物学报), 11, 123-126. (in Chinese with English abstract)
[48]	Ye WH, Li J, Cao HL, Ge XJ (2003). Genetic uniformity of Alternanthera philoxeroides in South China. Weed Research, 43, 297-302. DOI URL
[49]	Ye WH, Mu HP, Cao HL, Ge XJ (2004). Genetic structure of the invasive Chromolaena odorata in China. Weed Research, 44, 129-135. DOI URL
[50]	Yeh FC, Boyle TJB (1997). Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian Journal of Botany, 129, 157.
[51]	Zan QJ(昝启杰), Wang BS(王伯荪), Wang YJ(王勇军), Liao WB(廖文波), Li MG(李鸣光), Xu HL(徐华林) (2002). The ecological evaluation on the controlling Mikania micrantha by Cuscuta campestris. Acta Scientiarum Natralium Universitis Sunyatseni (中山大学学报(自然科学版)), 41(6), 60-63. (in Chinese with English abstract)
[52]	Zan QJ(昝启杰), Wang YJ(王勇军), Wang BS (王伯荪), Liao WB (廖文波), Li MG (李鸣光) (2000). The distribution and harm of the exotic weed Mikania micarantha. Chinese Journal of Ecology (生态学杂志), 19(6), 58-61. (in Chinese with English abstract)
[53]	Zhang FM(张富民), Ge S(葛颂) (2002). Data analysis in population genetics. I. Analysis of RAPD data with AMOVA. Biodiversity Science (生物多样性), 10, 438-444. (in Chinese with English abstract)
[54]	Zhang WY(张炜银), Wang BS(王伯荪), Li MG(李鸣光), Zan QJ(昝启杰), Wang YJ(王勇军) (2002a). The effects of light intensity on growth and morphology in Mikania micrntha seedlings. Sun Yatsen University Forum (中山大学学报论丛), 22(1), 222-226. (in Chinese with English abstract)
[55]	Zhang WY(张炜银), Wang BS(王伯荪), Liao WB(廖文波), Li MG(李鸣光), Wang YJ(王勇军), Zan QJ(昝启杰) (2002b). Progress in studies on an exotic vicious weed Mikania micrantha. Chinese Journal of Applied Ecology (应用生态学报), 13, 1684-1688. (in Chinese with English abstract)
[56]	Zhang WY(张炜银), Wang BS(王伯荪), Zhang JL(张军丽), Li MG(李鸣光), Zan QJ(昝启杰), Wang YJ(王勇军) (2002c). Study on the structure and dynamics of seedlings of Mikania micrantha populations. Acta Scientiarum Naturalium Universitis Sunyatseni (中山大学学报(自然科学版)), 41(6), 64-66. (in Chinese with English abstract)
[57]	Zietkiewicz E, Rafalski A, Labuda D (1994). Genome fingerprinting by simple sequence repeat (SSR)-anhorcd polymerase chain reaction amplification. Genomics, 20, 176-183. DOI URL PMID

引物 Primers	序列 Sequences	引物 Primers	序列 Sequences
UBC808	(AG)₈C	UBC873	(GACA)₄
UBC811	(GA)₈C	UBC880	(GGAGA)₃
UBC841	(GA)₈YC	UBC884	HBH(AG)₇
UBC857	(AC)₈YG	UBC885	BHB(GA)₇
UBC864	(ATG)₆	UBC886	VDV(CT)₇
UBC868	(GAA)₆	UBC887	DVD(TC)₇

引物 Primers	序列 Sequences	引物 Primers	序列 Sequences
UBC808	(AG)₈C	UBC873	(GACA)₄
UBC811	(GA)₈C	UBC880	(GGAGA)₃
UBC841	(GA)₈YC	UBC884	HBH(AG)₇
UBC857	(AC)₈YG	UBC885	BHB(GA)₇
UBC864	(ATG)₆	UBC886	VDV(CT)₇
UBC868	(GAA)₆	UBC887	DVD(TC)₇

种群(代码) Population(Code)	多态位点数 Number of polymorphic loci	多态位点百分率(%) Percetage of polymorphic loci (P)	Shannon信息指数 Shannon's information index (I)	Nei基因多样性 Nei's genet diversity (h)
西湖乐园 (XH)	69	40.35	0.233 5	0.159 8
民俗村 (MS)	59	34.50	0.179 1	0.118 9
大王山 (DWS)	62	36.26	0.191 2	0.129 5
大望村 (DW)	58	33.92	0.190 1	0.128 4
内伶仃岛I (NLD I)	55	32.16	0.155 2	0.102 6
内伶仃岛II (NLD II)	57	33.33	0.165 8	0.110 8
梧桐山 (WT)	58	33.92	0.164 0	0.108 2
去梧桐山公路边 (GL)	55	32.16	0.152 2	0.099 7
种Speices	103	60.23	0.281 8	0.184 9

种群(代码) Population(Code)	多态位点数 Number of polymorphic loci	多态位点百分率(%) Percetage of polymorphic loci (P)	Shannon信息指数 Shannon's information index (I)	Nei基因多样性 Nei's genet diversity (h)
西湖乐园 (XH)	69	40.35	0.233 5	0.159 8
民俗村 (MS)	59	34.50	0.179 1	0.118 9
大王山 (DWS)	62	36.26	0.191 2	0.129 5
大望村 (DW)	58	33.92	0.190 1	0.128 4
内伶仃岛I (NLD I)	55	32.16	0.155 2	0.102 6
内伶仃岛II (NLD II)	57	33.33	0.165 8	0.110 8
梧桐山 (WT)	58	33.92	0.164 0	0.108 2
去梧桐山公路边 (GL)	55	32.16	0.152 2	0.099 7
种Speices	103	60.23	0.281 8	0.184 9

变异来源 Source of variance	自由度 df	变异组分 Variance component	百分率 Pcentage	p值 p value^*
种群间Among popualtions	7	5.257 7	36.49	<0.001
种群内Within populations	152	9.150 7	63.51	<0.001