Chin J Plant Ecol ›› 2010, Vol. 34 ›› Issue (12): 1433-1438.DOI: 10.3773/j.issn.1005-264x.2010.12.009
• Research Articles • Previous Articles Next Articles
HOU Tian-Wen1, JIN Hui2, LIU Hong-Xia1,*(), LUO Yi-Bo2
Received:
2010-05-20
Accepted:
2010-08-31
Online:
2010-05-20
Published:
2010-12-28
Contact:
LIU Hong-Xia
HOU Tian-Wen, JIN Hui, LIU Hong-Xia, LUO Yi-Bo. Mycorrhizal specificity of Doritis pulcherrima in in-vitro research[J]. Chin J Plant Ecol, 2010, 34(12): 1433-1438.
阶段 Stage | 描述 Description |
---|---|
0 | 未萌发 No germination |
1 | 种胚膨大 Swelling of the embryo |
2 | 基毛出现 Development of radical hairs |
3 | 叶原基出现 Development of a leaf primodium |
4 | 第一片叶子出现 Development of the first leaf |
5 | 第二片叶子出现 Development of the second leaf |
6 | 根萌发 Development of roots |
Table 1 Germination stages of orchid seeds in vitro (Cited from Otero et al., 2004)
阶段 Stage | 描述 Description |
---|---|
0 | 未萌发 No germination |
1 | 种胚膨大 Swelling of the embryo |
2 | 基毛出现 Development of radical hairs |
3 | 叶原基出现 Development of a leaf primodium |
4 | 第一片叶子出现 Development of the first leaf |
5 | 第二片叶子出现 Development of the second leaf |
6 | 根萌发 Development of roots |
Fig. 1 Symbiotic seeds, protocorms and seedlings of Doritis pulcherrima after 20 weeks. A, Successfully germinated seed with fungi strain Mm1. B, Excellently developed protocorms with fungi strain Mm4. C, Seedlings comparison between well-growing inoculation samples with fungi strain Mm1 and control treatments.
处理 Treatment | 鉴定信息 Identified information | 种子萌发 Seeds germination | 原球茎分化 Protocorm differentiation | 处理苗平均鲜重增长率 Average increasement of fresh mass (%) |
---|---|---|---|---|
Control | 无真菌处理 No fungus treatment | × | 无明显分化 No obvious differentiation | 156.25 |
Cf1 | Cryptosporiopsis radicicola | √ | 死亡 Dead | 0 |
Cf2 | 未知真菌 Unknown fungus | × | 同对照 As control treatment | 86.10 |
Ct1 | 康宁木霉 Trichoderma koningii | × | 死亡 Dead | 265.89 |
Ct2 | 冬虫夏草 Cordyceps sinensis | × | 同对照 As control treatment | 185.98 |
Ct3 | 胶膜菌 Tulasnella sp. | × | 死亡 Dead | 272.22 |
Gd1 | 绿色肉座菌 Hypocrea virens | × | 死亡 Dead | 177.08 |
Mm1 | 瘤菌根菌 Epulorhiza sp. | √ | 被污染 Polluted | 619.84** |
Mm2 | 烧地环锈伞 Pholiota carbonaria | × | 同对照 As control treatment | 135.10 |
Mm3 | 帚状弯孢聚壳 Eutypella scoparia | × | 死亡 Dead | 51.87 |
Mm4 | 丛赤壳科真菌 Nectriaceae sp. | × | 分化旺盛 Well differentiated | 110.42 |
Na1 | 杜鹃菌根菌 Ericoid mycorrhizal fungus | × | 分化旺盛 Well differentiated | 112.70 |
Na2 | 柱捕单顶孢菌 Monacrosporium cionopagum | × | 分化旺盛 Well differentiated | 79.80 |
Na3 | 未知担子菌 Unknown basidiomycete | × | 分化旺盛 Well differentiated | 0 |
Pc1 | 毛霉属真菌 Mortierella sp. | × | 死亡 Dead | 302.67 |
Pd1 | 胶膜菌 Tulasnellaceae isolate | × | 分化旺盛 Well differentiated | 285.00 |
Pd2 | 镰刀菌 Fusarium tricinctum | × | 分化旺盛 Well differentiated | 65.50 |
Pm1 | 炭角菌 Xylaria arbuscula | × | 死亡 Dead | 0 |
Ts1 | 丝核菌 Rhizoctonia sp. | × | 分化旺盛 Well differentiated | 201.80 |
Ts2 | 柔膜菌目外生菌根真菌 Ectomycorrhizal fungi of Helotiales | × | 分化旺盛 Well differentiated | 161.10 |
Ts3 | 粪盘菌 Ascobolus crenulatus | × | 同对照 As control treatment | 266.67 |
Ts4 | Creosphaeria sassafras | × | 分化旺盛 Well differentiated | 347.04 |
Ts5 | 奥氏蜜环菌 Armillaria ostoyae | × | 死亡 Dead | 145.90 |
Table 2 Inoculation results of Doritis pulcherrima with the endophytic fungi of temperate terrestrial orchid
处理 Treatment | 鉴定信息 Identified information | 种子萌发 Seeds germination | 原球茎分化 Protocorm differentiation | 处理苗平均鲜重增长率 Average increasement of fresh mass (%) |
---|---|---|---|---|
Control | 无真菌处理 No fungus treatment | × | 无明显分化 No obvious differentiation | 156.25 |
Cf1 | Cryptosporiopsis radicicola | √ | 死亡 Dead | 0 |
Cf2 | 未知真菌 Unknown fungus | × | 同对照 As control treatment | 86.10 |
Ct1 | 康宁木霉 Trichoderma koningii | × | 死亡 Dead | 265.89 |
Ct2 | 冬虫夏草 Cordyceps sinensis | × | 同对照 As control treatment | 185.98 |
Ct3 | 胶膜菌 Tulasnella sp. | × | 死亡 Dead | 272.22 |
Gd1 | 绿色肉座菌 Hypocrea virens | × | 死亡 Dead | 177.08 |
Mm1 | 瘤菌根菌 Epulorhiza sp. | √ | 被污染 Polluted | 619.84** |
Mm2 | 烧地环锈伞 Pholiota carbonaria | × | 同对照 As control treatment | 135.10 |
Mm3 | 帚状弯孢聚壳 Eutypella scoparia | × | 死亡 Dead | 51.87 |
Mm4 | 丛赤壳科真菌 Nectriaceae sp. | × | 分化旺盛 Well differentiated | 110.42 |
Na1 | 杜鹃菌根菌 Ericoid mycorrhizal fungus | × | 分化旺盛 Well differentiated | 112.70 |
Na2 | 柱捕单顶孢菌 Monacrosporium cionopagum | × | 分化旺盛 Well differentiated | 79.80 |
Na3 | 未知担子菌 Unknown basidiomycete | × | 分化旺盛 Well differentiated | 0 |
Pc1 | 毛霉属真菌 Mortierella sp. | × | 死亡 Dead | 302.67 |
Pd1 | 胶膜菌 Tulasnellaceae isolate | × | 分化旺盛 Well differentiated | 285.00 |
Pd2 | 镰刀菌 Fusarium tricinctum | × | 分化旺盛 Well differentiated | 65.50 |
Pm1 | 炭角菌 Xylaria arbuscula | × | 死亡 Dead | 0 |
Ts1 | 丝核菌 Rhizoctonia sp. | × | 分化旺盛 Well differentiated | 201.80 |
Ts2 | 柔膜菌目外生菌根真菌 Ectomycorrhizal fungi of Helotiales | × | 分化旺盛 Well differentiated | 161.10 |
Ts3 | 粪盘菌 Ascobolus crenulatus | × | 同对照 As control treatment | 266.67 |
Ts4 | Creosphaeria sassafras | × | 分化旺盛 Well differentiated | 347.04 |
Ts5 | 奥氏蜜环菌 Armillaria ostoyae | × | 死亡 Dead | 145.90 |
[1] | Arditti J, Ernst R, Yam TW, Glabe C (1990). The contributions of orchid mycorrhizal fungi to seed germination: a speculative review. Lindleyana, 5, 249-255. |
[2] | Bayman P, Gonzalez EJ, Fumero JJ, Tremblay RL (2002). Are fungi necessary? How fungicides affect growth and survival of the orchid Lepanthes rupestris in the field. Journal of Ecology, 90, 1002-1008. |
[3] | Bonnardeaux Y, Brundrett M, Batty A (2007). Diversity of mycorrhizal fungi of terrestrial orchids: compatibility webs, brief encounters, lasting relationships and alien invasions. Mycological Research, 3, 51-61. |
[4] | Chen JH (陈金花), Hu MJ (胡美娇), Song XQ (宋希强), He MG (何明高), Luo YB (罗毅波) (2010). Microscopic observation on mycorrhiza of Doritis pulcherrima. Mycosystema (菌物学报), 29, 26-30. (in Chinese with English abstract) |
[5] | Chen XC (陈心启), Tsi ZH (吉占和) (1998). The Chinese Orchids (中国兰花全书). China Forestry Publishing House, Beijing. (in Chinese) |
[6] |
Dearnaley JDW (2007). Further advances in orchid mycorrhizal research. Mycorrhiza, 17, 475-486.
DOI URL PMID |
[7] | Dijk E, Willems JH, van Andel J (1997). Nutrient responses as a key factor to the ecology of orchid species. Acta Botanica Neerlandica, 46, 339-363. |
[8] | Fan L (范黎), Guo SX (郭顺星) (1998). Research development of orchid mycorrhizal fungi. Microbiology (微生物学通报), 24, 227-230. (in Chinese with English abstract) |
[9] | He MG (何明高), Song SQ (宋松泉), Song XQ (宋希强), Wang RX (王瑞霞) (2009). A Kind of Testing Method of Orchid Seeds Vitality (一种兰花种子活力的测试方法). China Patent No.: 200810118434.4. 2009. 4. 8 |
[10] | Hou TW (侯天文) (2010). Mycorrhizal Fungi Diversity of the Dominant Orchids in the Huanglong Valley, Sichuan (四川黄龙沟优势兰科植物菌根真菌多样性). Master Dissertation, Beijing Forestry University, Beijing. (in Chinese with English abstract) |
[11] | Ke HL (柯海丽), Song XQ (宋希强), Tan ZQ (谭志琼), Liu HX (刘红霞), Luo YB (罗毅波) (2007). Endophytic fungi diversity in root of Doritis pulcherrima (Orchidaceae). Biodiversity Science (生物多样性), 15, 456-462. (in Chinese with English abstract) |
[12] | Ke HL (柯海丽), Song XQ (宋希强), Luo YB (罗毅波), Zhu GP (朱国鹏), Ling XB (凌绪柏) (2008). Seedling cultivation of Doritis pulcherrima Lindl. with mycorrhizal fungi. Acta Horticulturae Sinica (园艺学报), 35, 571-576. (in Chinese with English abstract) |
[13] |
Otero JT, Ackerman JD, Bayman P (2002). Diversity and host specificity of endophytic Rhizoctonia-like fungi from tropical orchids. American Journal of Botany, 89, 1852-1858.
DOI URL PMID |
[14] |
Otero JT, Ackerman JD, Bayman P (2004). Differences in mycorrhizal preferences between two tropical orchids. Molecular Ecology, 13, 2393-2404.
DOI URL PMID |
[15] |
Otero JT, Flanagan NS, Herre EA, Ackerman JD, Bayman P (2007). Widespread mycorrhizal specificity correlates to mycorrhizal function in the neotropical, epiphytic orchid Ionopsis utricularioides(Orchidaceae). American Journal of Botany, 94, 1944-1950.
DOI URL PMID |
[16] | Rasmussen HN (2002). Recent development in the study of OM. Plant and Soil, 244, 149-163. |
[17] | Rasmussen HN, Whigham DF (1998). The underground phase: a special challenge in studies of terrestrial orchid populations. Botanical Journal of the Linnean Society, 126, 49-64. |
[18] |
Suárez JP, Weiß M, Abele A, Garnica S, Oberwinkler F, Kottke I (2006). Diverse tulasnelloid fungi form mycorrhizas with epiphytic orchids in an Andean cloud forest. Mycological Research, 110, 1257-1270.
DOI URL PMID |
[19] | Tan LY, Aung T, Yam TW (2004). Studies on the growth rate of tropical orchid seeds in symbiotic cultures. http://staff.science.nus.edu.sg/~scilooe/srp_2003/sci_paper/botanic/research_paper/tan_liyang.pdf. Cited July 10, 2009. |
[20] |
Taylor DL, Bruns TD (1999). Population, habitat and genetic correlates of mycorrhizal specialization in the ‘cheating’ orchids Corallorhiza maculate and C. mertensiana. Molecular Ecology, 8, 1719-1732.
URL PMID |
[21] | Tremblay RL, Ackerman JD, Zimmerman JK, Calvo RN (2004). Variation in sexual reproduction in orchids and its evolutionary consequences: a spasmodic journey to diversification. Biological Journal of the Linnean Society, 84, 1-54. |
[22] | Warcup JH (1981). The mycorrhizal relationships of Australian orchids. New Phytologist, 87, 371-381. |
[23] | Zettler LW, Burkhead JC, Marshall JA (1999). Use of a mycorrhizal fungus from Epidendrum conopseum to germinate seed of Encyclia tampensis in vitro. Lindleyana, 14, 102-105. |
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