Allozyme variation of genetic diversity in natural populations of Jatropha curcas germplasm from different areas in southwest China

doi:10.3724/SP.J.1258.2011.00330

Abstract

Abstract:

Aims Our objectives was to investigate the genetic structure and diversity of ten natural populations of Jatropha curcas.
Methods Seven loci encodings were detected by vertical polyacrylamide gel electrophoresis.
Important findings The genetic diversity of J. curcas was high at the species level with a mean number of alleles per locus of 2.428 6, percentage of polymorphic loci of 97.14% and a mean expected heterozygosity of 0.396 4. A low level of genetic differentiation among populations (0.041 3) and a high estimate of gene flow (5.808 9) were detected together with a high level of genetic identity among populations (0.921 7-0.995 3). Unweighted pair-group mean arithmetic cluster analysis suggested that the genetic distances among populations were weakly correlated with their geographic distances. The results provided a good genetic base to select varieties.

Key words: allozyme, genetic diversity, Jatropha curcas

ZHANG Wei, LUO Jian-Xun, GU Yun-Jie, HU Ting-Xing. Allozyme variation of genetic diversity in natural populations of Jatropha curcas germplasm from different areas in southwest China[J]. Chin J Plant Ecol, 2011, 35(3): 330-336.

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URL: https://www.plant-ecology.com/EN/10.3724/SP.J.1258.2011.00330

https://www.plant-ecology.com/EN/Y2011/V35/I3/330

Figures/Tables 8

References 20

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种群代号 Population code	采样地 Seed collection site	经纬度 Longitude and latitude	海拔 Altitude (m)	水系 River system	家系数量 No. of families
LS	卢水 Lushui	98°08′ E, 25°08′ N	850	怒江 Nujiang River	30
SB	双柏 Shuangbo	101°51′ E, 25°49′ N	1 066	红河 Honghe River	30
YNPE	普洱 Puer	100°59′ E, 22°42′ N	1 300	澜沧江 Lancang River	30
XSBN	西双版纳 Xishuangbanna	100°80′ E, 22°35′ N	1 000	澜沧江 Lancang River	30
PJ	坡脚 Pojiao	102°55′ E, 26°74′ N	1 080	金沙江 Jinsha River	30
FY	泸西 Luxi	103°76′ E, 24°52′N	1 100	南盘江 Nanpan River	30
LB	雷波 Leibo	103°62′ E, 28°21′ N	960	金沙江 Jinsha River	30
HPZ	花棚子 Huapengzi	101°55′ E, 26°22′ N	983	金沙江 Jinsha River	30
HL	会理 Huili	101°55′ E, 26°10′ N	943	金沙江 Jinsha River	30
YL	金河 Jinhe	101°51′ E, 27°42′ N	1 200	雅砻江 Yagong River	30

种群代号 Population code	采样地 Seed collection site	经纬度 Longitude and latitude	海拔 Altitude (m)	水系 River system	家系数量 No. of families
LS	卢水 Lushui	98°08′ E, 25°08′ N	850	怒江 Nujiang River	30
SB	双柏 Shuangbo	101°51′ E, 25°49′ N	1 066	红河 Honghe River	30
YNPE	普洱 Puer	100°59′ E, 22°42′ N	1 300	澜沧江 Lancang River	30
XSBN	西双版纳 Xishuangbanna	100°80′ E, 22°35′ N	1 000	澜沧江 Lancang River	30
PJ	坡脚 Pojiao	102°55′ E, 26°74′ N	1 080	金沙江 Jinsha River	30
FY	泸西 Luxi	103°76′ E, 24°52′N	1 100	南盘江 Nanpan River	30
LB	雷波 Leibo	103°62′ E, 28°21′ N	960	金沙江 Jinsha River	30
HPZ	花棚子 Huapengzi	101°55′ E, 26°22′ N	983	金沙江 Jinsha River	30
HL	会理 Huili	101°55′ E, 26°10′ N	943	金沙江 Jinsha River	30
YL	金河 Jinhe	101°51′ E, 27°42′ N	1 200	雅砻江 Yagong River	30

酶系统 Enzyme system	缩写 Abbreviation	标准代号 EC No.	位点数 No. of loci
谷草转氨酶 Glutamic-oxalacetic transaminase	GOT	(E.C.2.6.1.1)	2
莽草酸脱氢酶 Shikimate acid dehydrogenase	SHDH	(E.C.1.1.1.25)	1
6-磷酸葡萄糖脱氢酶 Phosphoglucose dehydrogenase	6PGD	(E.C 1.1.1.44)	2
丙氨酸氨基肽酶 Alanine aminopeptidase	AAP	(E.C.3.4.11. I)	2
磷酸葡萄糖变位酶 Phosphogluco mutase	PGM	(E.C.5.4.2.23)	3
磷酸葡萄糖异构酶 Phosphogluco isomerase	PGI	(E.C.5.3.1.9)	4
甲基萘醌还原酶 Menadione reductase	MNR	(E.C.1.6.99.2)	7

酶系统 Enzyme system	缩写 Abbreviation	标准代号 EC No.	位点数 No. of loci
谷草转氨酶 Glutamic-oxalacetic transaminase	GOT	(E.C.2.6.1.1)	2
莽草酸脱氢酶 Shikimate acid dehydrogenase	SHDH	(E.C.1.1.1.25)	1
6-磷酸葡萄糖脱氢酶 Phosphoglucose dehydrogenase	6PGD	(E.C 1.1.1.44)	2
丙氨酸氨基肽酶 Alanine aminopeptidase	AAP	(E.C.3.4.11. I)	2
磷酸葡萄糖变位酶 Phosphogluco mutase	PGM	(E.C.5.4.2.23)	3
磷酸葡萄糖异构酶 Phosphogluco isomerase	PGI	(E.C.5.3.1.9)	4
甲基萘醌还原酶 Menadione reductase	MNR	(E.C.1.6.99.2)	7

位点 Locus	等位基因 Allele	种群 Population
位点 Locus	等位基因 Allele	金河 YL	坡脚 PJ	花棚子 HPZ	雷波 LB	法依 FY	双柏 SB	版纳 XSBN	普洱 YNPE	会理 HL	卢水 LS	平均 Mean
AAP-1	A	0.725	0.675	0.650	0.750	0500	0.675	0.600	0.750	0.550	0.650	0.652
	B	0.275	0.325	0.350	0.250	0.500	0.325	0.400	0.250	0.450	0.350	0.348
SHDH	A	0.300	0.350	0.300	0.600	0.600	0.775	0.600	0.500	0.525	0.325	0.487
	B	0.675	0.500	0.500	0.400	0.400	0.175	0.400	0.500	0.450	0.675	0.467
	C	0.025	0.150	0.200	0.000	0.000	0.050	0.000	0.000	0.025	0.000	0.045
GOT-2	A	0.425	0.375	0.325	0.350	0.350	0.350	0.300	0.400	0.325	0.275	0.347
	B	0.575	0.625	0.675	0.650	0.650	0.650	0.700	0.600	0.625	0.725	0.647
PGI-4	A	0.625	0.500	0.450	0.550	0.475	0.525	0.575	0.500	0.550	0.550	0.530
	B	0.375	0.500	0.500	0.375	0.475	0.350	0.425	0.500	0.450	0.450	0.440
	C	0.000	0.000	0.050	0.075	0.050	0.125	0.000	0.000	0.000	0.000	0.030
6PGD-1	A	1.000	0.600	0.700	0.875	0.875	0.750	0.650	0.800	0.750	1.000	0.800
	B	0.000	0.400	0.300	0.125	0.125	0.250	0.350	0.200	0.250	0.000	0.200
MNR-3	A	0. 250	0.175	0.175	0.200	0.175	0.150	0.200	0.275	0.250	0.175	0.177
	B	0.750	0.825	0.825	0.800	0.825	0.850	0.800	0.725	0.750	0.825	0.797
PGM-2	A	0.700	0.875	0.850	0.700	0.825	0.650	0.700	0.625	0.575	0.675	0.718
	B	0.300	0.125	0.150	0.300	0.175	0.325	0.300	0.375	0.425	0.325	0.280
	C	0.000	0.000	0.000	0.000	0.000	0.025	0.000	0.000	0.000	0.000	0.002
基因频率平均值 Mean value of gene frequency		0.397	0.412	0.412	0.412	0.412	0.412	0.412	0.412	0.409	0.412	0.410
等位基因数 Number of alleles		14	15	16	15	15	17	15	14	15	13	14.9