Analysis of the relationship between cyanide-resistant respiration and photosynthesis under light in Phaseolus vulgaris leaves

doi:10.17521/cjpe.2015.0011

Abstract

Abstract: Aims

In order to further understand the inner mechanism of plant respiration and the relationship between respiration and photosynthesis of plant under light, our objective was to reveal the relationship between cyanide-resistant respiration and photosynthesis in bean (Phaseolus vulgaris) leaves under light.

<i>Methods</i>

By exposing the dark-grown leaves to light for 10 h, changes in total respiration (V_t), the cyanide-resistant respiration (V_alt), photosynthetic O₂evolution, and photosynthetic CO₂ fixation, and the effects of inhibitor of the cyanide-resistant respiration on photosynthesis were measured and analyzed. We also measured and analyzed changes in the ratio of cyanide-resistant respiration, total respiration, and photosynthetic CO₂ fixation when leaves in the dark were exposed to a brief period (10 min) of light. Important findings After exposing the dark-grown leaves to light for 10 h, V_t, V_alt, and the value of V_alt/V_t all increased. During the process, time-course analysis after the onset of illumination demonstrated that the induction of the cyanide-resistant respiration in light was prior to the formations of the photosynthetic O₂evolution and CO₂ fixation. This observation indicates that the induction of the cyanide-resistant respiration by light is independent of photosynthesis. The dark-grown leaves pretreated with 1 mmol·L^-1 salicylhydroxamic acid (SHAM; the inhibitor of the cyanide-resistant respiration) were exposed to illumination for 10 h, and SHAM did not result in apparent modification of the photosynthetic O₂evolution and CO₂ fixation in the leaves when exposed to light. This observation also showed that there was no direct linkage between photosynthesis and the cyanide-resistant respiration when the dark-grown leaves were exposed to light. In addition, under the condition of darkness, 10 min of light illumination obviously increased the value of V_alt/V_t but did not significantly affect the level of photosynthetic CO₂ fixation.

Results

indicate that the induction of the cyanide-resistant respiration by light may be independent of photosynthesis. Light should have a direct influence on induction of the cyanide-resistant respiration.

Key words: cyanide-resistant respiration, light illumination, photosynthesis

FENG Han-Qing,GUAN Dong-Dong,JIAO Qing-Song,JIA Ling-Yun,SUN Kun. Analysis of the relationship between cyanide-resistant respiration and photosynthesis under light in Phaseolus vulgaris leaves[J]. Chin J Plan Ecolo, 2015, 39(1): 104-109.

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https://www.plant-ecology.com/EN/Y2015/V39/I1/104

Figures/Tables 5

Fig. 1 Changes in total respiration (Vt), capacity of cyanide- resistant respiration (Valt) and the ratio of Valt to Vt in dark- grown leaves exposed to continuous light for 10 h. These are individual samples taken during four different experiments. Time indicates hours after starting illumination. Values are mean values ± SD of four independent experiments. The horizontal axis shows the time after exposing to light. Valt/Vt was computed based on the average values of Vt and Valt.

Fig. 2 Alternative oxidase (AOX) transcript levels during 10 h of continuous illumination.

Fig. 3 Changes in oxygen evolution (■) and carbon dioxide fixation (□) in whole chloroplasts of the dark-grown leaves exposed to 10 h of continuous light. Values are mean values ± SD of four independent experiments. The horizontal axis shows the time after exposing to light.

Table 1 The effects of salicylhydroxamic acid (SHAM) on photosynthetic oxygen evolution rate and carbon dioxide fixation rate when the dark-grown leaves were exposed to continuous light

光照的时间 Time of illumination (h)	SHAM对于光合作用的影响 Effects of SHAM on photosynthesis (% of control)
光照的时间 Time of illumination (h)	光合放氧 Oxygen evolution	光合CO₂固定 CO₂fixation
0	100^a	100^a
2	100^a	100^a
4	98 ± 5^a	100^a
6	101 ± 3^a	100^a
8	97 ± 4^a	96 ± 4^a
10	102 ± 4^a	95 ± 3^a

Fig. 4 Effect of short period of illumination on the ratio of cyanide-resistant respiration pathway to total respiration (Valt/Vt, %) (■) and photosynthetic CO2 fixation rate (□). One-week-old plants grown in 12 h light/12 h dark photoperiods were transferred to darkness and then received 10 min plus of light once every 12 h. Leaves in darkness were assigned the values of control (100%). Time indicates hours in darkness. Results are mean values ± SD of four independent experiments.

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