Chin J Plant Ecol ›› 2019, Vol. 43 ›› Issue (7): 576-584.doi: 10.17521/cjpe.2019.0009

• Research Articles • Previous Articles     Next Articles

13C pulse labeling reveals the effects of grazing on partitioning of assimilated carbon in an alpine meadow

CHEN Jin1,2,SONG Ming-Hua1,*(),LI Yi-Kang3   

  1. 1Key Laboratory of Ecosystem Network Observation and Modeling, Institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China
    2University of Chinese Academy of Sciences, Beijing 100049, China
    3Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China
  • Received:2019-01-10 Accepted:2019-06-25 Online:2019-12-12 Published:2019-07-20
  • Contact: SONG Ming-Hua E-mail:songmh@igsnrr.ac.cn
  • Supported by:
    Supported by the National Key R&D Program of China(2016YFC0502001);Supported by the National Key R&D Program of China(2016YFC0501803);the National Natural Science Foundation of China(41671263)

Abstract:

Aims In this study, we aim to understand how grazing would influence the partitioning of assimilated carbon in an alpine meadow on the Qinghai-Xizang Plateau.
Methods Measurements on carbon partitioning were made in a long-term grazing experiment consisting of light winter grazing and enclosure treatments. The 13C tracer method was used to determine the partitioning and transportation of assimilated carbon into different carbon pools.
Important findings On the 30th day following the labeling, shoots retained 32% of the initial 13C, and roots and soil together retained 22%; about 30% of the initial 13C were lost through shoot respiration. There were significant differences in the retention in soil, and the respiratory emission from soil, of assimilated carbon between the light grazing and enclosure treatments. Under light grazing, plants invested more assimilated carbon into the root and soil carbon pools. The rate of 13C transportation from shoots to soil and the rate of respiratory 13C release from soil were both greater, and the retention of 13C in and respiratory release from shoots were lower, under light grazing than under enclosure. Our results suggest that grazing is an important mechanism for maintenance of grassland. Grazing may cause changes in the structure and functioning of ecosystems, and induce large variations in soil carbon storage. Alpine meadow in the Qinghai-Xizang Plateau is amongst the grasslands with highest elevation in the world, and has large soil carbon storage due to low temperatures. We found no difference in soil C stocks between light grazing and enclosure treatments, indicating that light grazing would have no significant impact on soil carbon stocks.

Key words: grazing, 13C isotope labeling, alpine meadow, partitioning of assimilated carbon

Fig. 1

Carbon (C) stock in soil (A) and plants (B) in light winter grazed (LWG) and fence-enclosed (Encl) alpine meadows and in plant shoot (C) and root (D) (mean ± SE). * indicates significant difference between grazed and ungrazed plots (p < 0.05)."

Fig. 2

Cumulative soil CO2-C efflux over 30 days following 13C labelling in light winter grazed (LWG) and fence-enclosed (Encl) alpine meadows(mean ± SE). * indicates significant difference between grazed and ungrazed plots (p < 0.05)."

Fig. 3

13C dynamics in shoots and roots and 13C losses by shoot respiration during a 30 day period following 13C labelling in light winter grazed (LWG) and fence-enclosed (Encl) alpine meadows (mean ± SE). * indicates significant difference between grazed and ungrazed plots (p < 0.05)."

Fig. 4

13C dynamics in roots and soil during a 30 day period following 13C labelling in light winter grazed (LWG) and fence- enclosed (Encl) alpine meadows (mean ± SE). * indicates significant difference between grazed and ungrazed plots (p < 0.05)."

Fig. 5

13CO2-C efflux rate in soil of light winter grazed (LWG) and fence-enclosed (Encl) alpine meadows during a 30 day period following 13C labelling (mean ± SE). * indicates significant difference between grazed and ungrazed plots (p < 0.05)."

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