冻融变化对西南亚高山森林优势种云杉和华西箭竹根区土壤理化性质与酶活性的影响

doi:10.17521/cjpe.2022.0031

植物生态学报 ›› 2023, Vol. 47 ›› Issue (6): 882-894.DOI: 10.17521/cjpe.2022.0031

• 研究论文 • 上一篇

冻融变化对西南亚高山森林优势种云杉和华西箭竹根区土壤理化性质与酶活性的影响

郭敏¹, 罗林², 梁进², 王彦杰¹^,^*(), 赵春章³^,^*()

¹四川师范大学生命科学学院, 成都 610100
²中国科学院成都生物研究所, 中国科学院山地生态恢复与生物资源利用重点实验室, 生态恢复与生物多样性保育四川省重点实验室, 成都 610041
³成都理工大学生态环境学院, 成都 610059

收稿日期:2022-01-18 接受日期:2022-05-20 出版日期:2023-06-20 发布日期:2022-06-09
通讯作者: * (Wang YJ, wyjilwm2015@163.com;Zhao CZ, zhaochzh04@126.com)
基金资助:
国家自然科学基金(31570477);国家自然科学基金(31700418);四川省科技计划项目(21ZDYF0754)

Effects of freeze-thaw changes on soil physicochemical properties and enzyme activities in root zone of Picea asperata and Fargesia nitida under subalpine forests of southwest China

GUO Min¹, LUO Lin², LIANG Jin², WANG Yan-Jie¹^,^*(), ZHAO Chun-Zhang³^,^*()

¹College of Life Science, Sichuan Normal University, Chengdu 610100, China
²Chengdu Institute of Biology, Chinese Academy of Sciences, CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu 610041, China
³College of Ecology and Environment, Chengdu University of Technology, Chengdu 610059, China

Received:2022-01-18 Accepted:2022-05-20 Online:2023-06-20 Published:2022-06-09
Contact: * (Wang YJ, wyjilwm2015@163.com;Zhao CZ, zhaochzh04@126.com)
Supported by:
National Natural Science Foundation of China(31570477);National Natural Science Foundation of China(31700418);Science and Technology Plan Projects in Sichuan Province(21ZDYF0754)

摘要/Abstract

摘要：

西南亚高山森林是典型的季节性冻土区, 为深入研究气候变暖背景下冻融循环变化对森林土壤环境的影响, 该研究以西南亚高山森林乔木层与灌木层优势种云杉(Picea asperata)和华西箭竹(Fargesia nitida)根区土壤为研究对象, 利用红外辐射加热器模拟气候变暖, 研究增温对非生长季土壤冻融循环、土壤理化性质和酶活性的影响。在此基础上, 开展室内培养实验, 进一步验证冻融循环变化对土壤性质的影响。结果表明: (1)与对照小区比较, 增温小区5 cm和15 cm土层温度分别升高2.85和2.13 ℃, 冻结天数分别减少了60和32天, 冻融循环次数分别由3次和1次降为0次。(2)增温增加了两物种根区土壤总氮(TN)、可溶性有机氮(DON)和微生物生物量氮(MBN)含量, 但降低了土壤铵态氮(NH₄⁺-N)含量。土壤冻结天数、冻融循环次数与TN、DON含量显著负相关, 与NH₄⁺-N含量显著正相关。(3)增温显著促进了两树种根区土壤N-乙酰-β-D-葡萄糖苷酶(NAG)活性, 但显著抑制了脲酶(Ure)活性。土壤冻结天数、冻融次数与NAG和Ure活性显著相关。(4)与野外研究相似, 室内冻融循环处理可显著增加云杉根区土壤NH₄⁺-N含量与β-葡萄糖苷酶(BG)活性, 降低了NAG活性; 增加了华西箭竹根区NH₄⁺-N含量, 降低了BG与NAG酶活性; 但冻融循环对土壤硝态氮(NO₃^--N)、DON含量、Ure及蛋白酶(Pro)活性的影响与野外研究结果不同。冗余分析表明, 华西箭竹根区土壤酶活性主要受土壤DON含量的影响, 而云杉根区土壤酶活性与pH、NH₄⁺-N含量、DON含量显著相关。以上结果说明, 气候变暖背景下季节性冻土冻融循环消失, 会显著影响西南亚高山森林非生长季土壤理化性质(尤其是土壤氮库组分)和酶活性, 但其影响机制需要进一步研究。

关键词: 冻融循环, 模拟增温, 云杉, 华西箭竹, 土壤理化性质, 酶活性

Abstract:

Aims The subalpine forests of southwest China belongs to a typical seasonal frozen soil area. This study aimed to explore the effects of freeze-thaw changes on the soil physicochemical properties and enzyme activities under forest soils in response to climate warming.

Methods Infrared radiation heaters were applied to simulate climate warming. Soils in the root zone of Picea asperata and Fargesia nitida were collected to explore the effects of warming on soil freeze-thaw cycles, soil physicochemical properties and enzyme activities. Meanwhile, soil incubation experiments were conducted to further analyze the effects of different freeze-thaw cycles on soil properties.

Important findings Our results showed that (1) Experimental warming increased the soil temperatures by 2.85 and 2.13 °C, decreased freezing days by 60 and 32 days, and also declined freeze-thaw cycles from 1-3 times to 0 time at the 5 cm and 15 cm soil depths, respectively. (2) Warming increased the contents of soil total nitrogen (TN), dissolved organic nitrogen (DON) and microbial biomass nitrogen (MBN), but significantly reduced the content of ammonium nitrogen (NH₄⁺-N) under both P. asperata and F. nitida plots. The soil freezing days and number of freeze-thaw cycles were significantly negatively correlated with TN and DON contents, and significantly positively correlated with NH₄⁺-N content. (3) Warming significantly promoted the activity of β-N-acetylglucosaminidase (NAG), but significantly inhibited the activity of urease (Ure) under the root zone soil of the two species. The activities of NAG and Ure were significantly correlated with freezing days and the number of freeze-thaw cycles. (4) Similar to results of the field study, the simulated freeze-thaw cycles significantly increased NH₄⁺-N content and 4-methylumbelliferyl-β-D-glucoside (BG) activity, but decreased NAG activity under the root zone soil of P. asperata. On the other hand, freeze-thaw cycles increased NH₄⁺-N content and reduced the activities of NAG and BG under the root zone soil of F. nitida. However, the effects of freeze-thaw cycles on the contents of nitrate nitrogen (NO₃^--N) and DON as well as the activities of Ure and protease (Pro) were different based on the results of field experiment. Redundancy analysis showed that soil enzyme activities under the root zone of F. nitida were mainly affected by DON content, whereas those parameters under the root zone of P. asperatawere significantly correlated with pH, NH₄⁺-N and DON contents. These results suggested that the disappearance of freeze-thaw cycles caused by future climate warming would significantly affect the soil physicochemical properties (especially soil nitrogen pool) and enzyme activities during non-growing season under subalpine forests of southwest China. But the underlying mechanism needs further study.

Key words: freeze-thaw cycle, simulated warming, Picea asperata, Fargesia nitida, soil physicochemical property, enzyme activity

郭敏, 罗林, 梁进, 王彦杰, 赵春章. 冻融变化对西南亚高山森林优势种云杉和华西箭竹根区土壤理化性质与酶活性的影响. 植物生态学报, 2023, 47(6): 882-894. DOI: 10.17521/cjpe.2022.0031

GUO Min, LUO Lin, LIANG Jin, WANG Yan-Jie, ZHAO Chun-Zhang. Effects of freeze-thaw changes on soil physicochemical properties and enzyme activities in root zone of Picea asperata and Fargesia nitida under subalpine forests of southwest China. Chinese Journal of Plant Ecology, 2023, 47(6): 882-894. DOI: 10.17521/cjpe.2022.0031

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2022.0031

https://www.plant-ecology.com/CN/Y2023/V47/I6/882

图/表 8

图1 西南亚高山森林样地5 cm土层土壤温度和湿度(2018-12-06至2019-03-31)。

Fig. 1 Temperature and humidity of 5 cm soil layer of sample plot under subalpine forests of southwest China (2018-12-06 to 2019-03-31).

表1 西南亚高山森林2018年12月至2019年3月增温和对照小区土壤冻融循环次数与时间(平均值±标准误)

Table 1 Freeze-thaw cycles of the warming and control plots during December 2018 to March 2019 under subalpine forests of China (mean ± SE)

处理 Treatment	土深5 cm Soil depth 5 cm		土深15 cm Soil depth 15 cm
处理 Treatment	冻结时间 Freezing time (d)	冻融循环次数 Freeze thaw cycle (times)	冻结时间 Freezing time (d)	冻融循环次数 Freeze thaw cycle (times)
对照 Control	65 ± 2.9	3 ± 0	36 ± 1.1	1 ± 0
增温 Warming	5 ± 0.6	0 ± 0	4 ± 0.5	0 ± 0

图2 增温对西南亚高山森林样地土壤理化性质的影响(平均值±标准误)。CK, 对照处理; W, 增温处理。FN, 华西箭竹; PA, 云杉。*, p < 0.05; ns, p > 0.05。

Fig. 2 Effects of warming on soil physicochemical properties of sample plot under subalpine forests of southwest China (mean ± SE). CK, control treatment; W, warming treatment. FN, Fargesia nitida; PA, Picea asperata. DON, dissolved organic nitrogen; MBN, microbial biomass nitrogen; NH4+-N, ammonium nitrogen; NO3--N, nitrate nitrogen. *, p < 0.05; ns, p > 0.05.

图3 增温对西南亚高山森林样地土壤酶活性的影响(平均值±标准误)。CK, 对照处理; W, 增温处理。 FN, 华西箭竹; PA, 云杉。BG, β-1,4-葡萄糖苷酶活性; NAG, N-乙酰-β-D-葡萄糖苷酶活性; Pro, 蛋白酶活性; Ure, 脲酶活性。*, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p > 0.05。

Fig. 3 Effects of warming on soil enzyme activities of sample plot under subalpine forests of southwest China (mean ± SE). CK, control treatments; W, warming treatments. FN, Fargesia nitida; PA, Picea asperata. BG, activity of 4-methylumbelliferyl-β-D-glucoside; NAG, activity of β-N-acetylglucosaminidase; Pro, activity of protease; Ure, activity of urease. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, p < 0.05.

表2 土壤冻融循环变化与土壤理化性质和酶活性的相关性分析

Table 2 Correlation analysis between soil physicochemical factors, enzyme activities and freeze-thaw cycles

	SM	pH	TC	TN	NO₃^--N	NH₄⁺-N	MBN	DON	BG	NAG	Pro	Ure
T5	-0.33	-0.27	0.08	-0.67^*	-0.38	0.72^**	-0.14	-0.62^**	0.94	-0.93^**	0.02	0.72^**
T15	-0.33	-0.27	0.08	-0.48^*	-0.51	0.83^**	-0.01	-0.85^**	0.889	-0.91^**	0.16	0.76^**
D5	-0.33	-0.30	0.01	-0.66^*	-0.43	0.80^**	-0.08	-0.71^**	0.906	-0.96^**	0.04	0.74^**
D15	-0.34	-0.29	0.02	-0.68^*	-0.42	0.79^**	-0.08	-0.69^**	0.898	-0.97^**	0.03	0.74^**

图4 冻融循环对西南亚高山森林样地土壤理化性质的影响(平均值±标准误)。0, 对照; 5, 5次冻融循环处理; 15, 15次冻融循环处理。FN, 华西箭竹; PA, 云杉。不同小写字母表示处理间差异显著(p < 0.05)。

Fig. 4 Effects of freeze-thaw cycles on soil physicochemical properties of sample plot under subalpine forests of southwest China (mean ± SE). 0, control treatment; 5, five freeze-thaw cycles; 15, fifteen freeze-thaw cycles. FN, Fargesia nitida; PA, Picea asperata. Different lowercase letters represent significant difference between treatments (p < 0.05).

图5 冻融循环对西南亚高山森林样地土壤酶活性的影响(平均值±标准误)。0, 对照; 5, 5次冻融循环处理; 15, 15次冻融循环处理。FN, 华西箭竹; PA, 云杉。BG, β-1,4-葡萄糖苷酶活性; NAG, N-乙酰-β-D-葡萄糖苷酶活性; Pro, 蛋白酶活性; Ure, 脲酶活性。不同小写字母表示处理间差异显著(p < 0.05)。

Fig. 5 Effects of freeze-thaw cycles on soil enzyme activities of sample plot under subalpine forests of southwest China (mean ± SE). 0, control treatment; 5, five freeze-thaw cycles; 15, fifteen freeze-thaw cycles. FN, Fargesia nitida; PA, Picea asperata. BG, activity of 4-methylumbelliferyl-β-D-glucoside; NAG, activity of β-N-acetylglucosaminidase; Pro, activity of protease; Ure, activity of urease. Different lowercase letters represent significant difference between treatments (p < 0.05).

图6 西南亚高山森林样地土壤酶活性与理化因子之间的冗余分析(RDA)。A, 华西箭竹。B, 云杉。BG, β-1,4-葡萄糖苷酶活性; DON, 可溶性有机氮含量; MBN, 微生物生物量氮含量; NAG, N-乙酰-β-D-葡萄糖苷酶活性; NH4+-N, 铵态氮含量; NO3--N, 硝态氮含量; Pro, 蛋白酶活性; SM, 土壤湿度; TC, 土壤总碳含量; TN, 土壤总氮含量; Ure, 脲酶活性。

Fig. 6 Redundancy analysis (RDA) of soil enzyme activities and physicochemical factors of sample plot under subalpine forests of southwest China. A, Fargesia nitida. B, Picea asperata. BG, activity of 4-methylumbelliferyl-β-D-glucoside; DON, dissolved organic nitrogen content; MBN, microbial biomass nitrogen content; NAG, activity of β-N-acetylglucosaminidase; NH4+-N, ammonium nitrogen content; NO3--N, nitrate nitrogen content; Pro, activity of protease; TC, total carbon content; TN, total nitrogen content; Ure, activity of urease.

参考文献 62

[1]	Bai E, Li SL, Xu WH, Li W, Dai WW, Jiang P (2013). A meta-analysis of experimental warming effects on terrestrial nitrogen pools and dynamics. New Phytologist, 199, 441-451.
[2]	Bai ZL, Wang BX, Lin JH (2021). Effect of freeze-thaw cycles on the bonding properties between BTRC textile and concrete. New Building Materials, 48(9), 36-40.
	[白子龙, 王伯昕, 林建宏 (2021). 冻融循环作用对玄武岩纤维编织网与混凝土粘结性能的影响. 新型建筑材料, 48(9), 36-40.]
[3]	Bell CW, Fricks BE, Rocca JD, Steinweg JM, McMahon SK, Wallenstein MD (2013). High-throughput fluorometric measurement of potential soil extracellular enzyme activities. Journal of Visualized Experiments, 81, e50961. DOI: 10.3791/50961.
[4]	Cai YJ, Wang XD, Ding WX, Yan Y, Lu XY, Du ZY (2013). Effects of freeze-thaw on soil nitrogen transformation and N₂O emission: a review. Acta Pedologica Sinica, 50, 1032-1042.
	[蔡延江, 王小丹, 丁维新, 鄢燕, 鲁旭阳, 杜子银 (2013). 冻融对土壤氮素转化和N₂O排放的影响研究进展. 土壤学报, 50, 1032-1042.]
[5]	Camill P (2005). Permafrost thaw accelerates in boreal peatlands during late-20th century climate warming. Climatic Change, 68, 135-152.
[6]	Campbell JL, Mitchell MJ, Groffman PM, Christenson LM, Hardy JP (2005). Winter in northeastern North America: a critical period for ecological processes. Frontiers in Ecology and the Environment, 3, 314-322.
[7]	Chang ZQ, Ma YL, Liu W, Feng Q, Su YH, Xi HY, Si JH (2014). Effect of soil freezing and thawing on the carbon and nitrogen in forest soil in the Qilian Mountains. Journal of Glaciology and Geocryology, 36, 200-206.
	[常宗强, 马亚丽, 刘蔚, 冯起, 苏永红, 席海洋, 司建华 (2014). 土壤冻融过程对祁连山森林土壤碳氮的影响. 冰川冻土, 36, 200-206.]
[8]	Chen ZH, Zhang XR, Tan B, Wei XY, Chen Y, Yang YL, Wu QG, Zhang L (2020). Effects of the freeze-thaw cycle on soil enzyme activities in a sub-alpine forest in western Sichuan. Acta Ecologica Sinica, 40, 2662-2669.
	[陈子豪, 张晓蓉, 谭波, 卫芯宇, 谌亚, 杨玉莲, 吴庆贵, 张丽 (2020). 冻融循环对川西亚高山森林土壤酶活性的影响. 生态学报, 40, 2662-2669.]
[9]	Cleavitt NL, Fahey TJ, Groffman PM, Hardy JP, Henry KS, Driscoll CT (2008). Effects of soil freezing on fine roots in a northern hardwood forest. Canadian Journal of Forest Research, 38, 82-91.
[10]	DeLuca TH, Keeney DR, McCarty GW (1992). Effect of freeze-thaw events on mineralization of soil nitrogen. Biology and Fertility of Soils, 14, 116-120.
[11]	Deng RJ, Yang WQ‚ Wu FZ (2009). Effects of seasonal freeze-thaw on the enzyme activities in Abies faxoniana and Betula platyphylla litters. Chinese Journal of Applied Ecology, 20, 1026-1031.
	[邓仁菊, 杨万勤, 吴福忠 (2009). 季节性冻融对岷江冷杉和白桦凋落物酶活性的影响. 应用生态学报, 20, 1026-1031.]
[12]	Djukic I, Kepfer-Rojas S, Schmidt IK, Larsen KS, Beier C, Berg B, Verheyen K (2018). Early stage litter decomposition across biomes. Science of the Total Environment, 628- 629, 1369-1394.
[13]	Du ZY (2020). Effects of freeze-thaw action on soil physicochemical and biological properties in the alpine grasslands. Ecology and Environmental Sciences, 29, 1054-1061.
	[杜子银 (2020). 冻融作用对高寒草地土壤理化和生物学性质的影响. 生态环境学报, 29, 1054-1061.]
[14]	Freeman C, Ostle N, Kang H (2001). An enzymic “latch” on a global carbon store. Nature, 409, 149. DOI: 10.1038/35051650.
[15]	Gilliam FS, Cook A, Lyter S (2010). Effects of experimental freezing on soil nitrogen dynamics in soils from a net nitrification gradient in a nitrogen-saturated hardwood forest ecosystem. Canadian Journal of Forest Research, 40, 436-444.
[16]	Han ZM, Deng MW, Yuan AQ, Wang JH, Li H, Ma JC (2018). Vertical variation of a black soil’s properties in response to freeze-thaw cycles and its links to shift of microbial community structure. Science of the Total Environment, 625, 106-113.
[17]	Hentschel K, Borken W, Matzner E (2008). Repeated freezen the microbial community structure explain the response of soil respiration to land-use change. Journal of Plant Nutrition and Soil Science, 171, 699-706.
[18]	Herrmann A, Witter E (2002). Sources of C and N contributing to the flush in mineralization upon freeze-thaw cycles in soils. Soil Biology & Biochemistry, 34, 1495-1505.
[19]	Hu CX, Tian ZW, Gu SL, Guo H, Fan YH, Abid M, Chen K, Jiang D, Cao WX, Dai TB (2018). Winter and spring night-warming improve root extension and soil nitrogen supply to increase nitrogen uptake and utilization of winter wheat (Triticum aestivum L.). European Journal of Agronomy, 96, 96-107.
[20]	IPCC (2018). Special Report on Global Warming of 1.5 °C. Cambridge University Press, Cambridge, UK.
[21]	Jefferies RL, Walker NA, Edwards KA, Dainty J (2010). Is the decline of soil microbial biomass in late winter coupled to changes in the physical state of cold soils? Soil Biology & Biochemistry, 42, 129-135.
[22]	Jones DL, Willett VB (2006). Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil. Soil Biology & Biochemistry, 38, 991-999.
[23]	Kang H, Freeman C (1999). Phosphatase and arylsulphatase activities in wetland soils: annual variation and controlling factors. Soil Biology & Biochemistry, 31, 449-454.
[24]	Koponen HT, Jaakkola T, Keinänen-Toivola MM, Kaipainen S, Tuomainen J, Servomaa K, Martikainen PJ (2006). Microbial communities, biomass, and activities in soils as affected by freeze thaw cycles. Soil Biology & Biochemistry, 38, 1861-1871.
[25]	Kreyling J, Schumann R, Weigel R (2020). Soils from cold and snowy temperate deciduous forests release more nitrogen and phosphorus after soil freeze-thaw cycles than soils from warmer, snow-poor conditions. Biogeosciences, 17, 4103-4117.
[26]	Larsen KS, Jonasson S, Michelsen A (2002). Repeated freeze-thaw cycles and their effects on biological processes in two Arctic ecosystem types. Applied Soil Ecology, 21, 187-195.
[27]	Li L, Wang JJ, Gao F, Fu MJ (2021). Effects of freeze-thaw cycles on soil nitrogen invertase of four temperate forests. Journal of Yangzhou University (Agricultural and Life Science Edition), 42, 111-118.
	[李龙, 王佳佳, 高峰, 傅民杰 (2021). 冻融循环对4种温带森林土壤氮转化酶的影响. 扬州大学学报(农业与生命科学版), 42, 111-118.]
[28]	Liu L, Zhu X, Sun G, Luo P, Wang B (2011). Effects of simulated warming and fertilization on activities of soil enzymes in alpine meadow. Pratacultural Science, 28, 1405-1410.
	[刘琳, 朱霞, 孙庚, 罗鹏, 王蓓 (2011). 模拟增温与施肥对高寒草甸土壤酶活性的影响. 草业科学, 28, 1405-1410.]
[29]	Liu Q, Wu Y, He H (2001). Ecological problems of subalpine coniferous forest in the southwest of China. World Sci-Tech R&D, 23, 63-69.
	[刘庆, 吴彦, 何海 (2001). 中国西南亚高山针叶林的生态学问题. 世界科技研究与发展, 23, 63-69.]
[30]	Lu BQ, Zang SY, Sun L (2019). The effects of freezing-thawing process on soil active organic carbon and nitrogen mineralization in Daxing’anling Mountain forests. Acta Scientiae Circumstantiae, 39, 1664-1672.
	[鲁博权, 臧淑英, 孙丽 (2019). 冻融作用对大兴安岭典型森林土壤活性有机碳和氮矿化的影响. 环境科学学报, 39, 1664-1672.]
[31]	Luo SQ, Fang XW, Lyu SH, Jiang Q, Wang JY (2017). Interdecadal changes in the freeze depth and period of frozen soil on the three rivers source region in China from 1960 to 2014. Advances in Meteorology, 2017, 5931467. DOI: 10.1155/2017/5931467.
[32]	Luo SQ, Wang JY, Pomeroy JW, Lyu SH (2020). Freeze-thaw changes of seasonally frozen ground on the Tibetan Plateau from 1960 to 2014. Journal of Climate, 33, 9427-9446.
[33]	Luo YC, LÜ YL, Yang H, He NP, Li SG, Gao WL (2014). Soil carbon and nitrogen mineralization in a Larix gmelinii forest during freeze-thaw cycles. Ecology and Environmental Sciences, 23, 1769-1775.
	[罗亚晨, 吕瑜良, 杨浩, 何念鹏, 李胜功, 高文龙 (2014). 冻融作用下寒温带针叶林土壤碳氮矿化过程研究. 生态环境学报, 23, 1769-1775.]
[34]	Ma YH, Liu YH, Wei WD (2018). Effects of freezing and thawing on soil organic carbon and components in degraded alpine meadow. Modern Agricultural Science and Technology, (20), 175-176.
	[马延虎, 刘育红, 魏卫东 (2018). 冻融作用对退化高寒草甸土壤有机碳及组分的影响. 现代农业科技, (20), 175-176.]
[35]	Mei LL, Yang X, Zhang SQ, Zhang T, Guo JX (2019). Arbuscular mycorrhizal fungi alleviate phosphorus limitation by reducing plant N:P ratios under warming and nitrogen addition in a temperate meadow ecosystem. Science of the Total Environment, 686, 1129-1139.
[36]	Nyberg L, Stähli M, Mellander P-E, Bishop KH (2001). Soil frost effects on soil water and runoff dynamics along a boreal forest transect: 1. Field investigations. Hydrological Processes, 15, 909-926.
[37]	Nazaries L, Tottey W, Robinson L, Khachane A, Al-Soud WA, Sørensen S, Singh BK (2015). Shifts in the microbial community structure explain the response of soil respiration to land-use change but not to climate warming. Soil Biology & Biochemistry, 89, 123-134.
[38]	Nikrad MP, Kerkhof LJ, Häggblom MM (2016). The subzero microbiome: microbial activity in frozen and thawing soils. FEMS Microbiology Ecology, 92, fiw081. DOI: 10.1093/femsec/fiw081.
[39]	Ouyang Q, Ren J, Yin J, Li YJ, Yuan FJ (2018). Effect of short-term simulated temperature enhancement on soil nutrients and urease activity of sub-alpine meadow. Pratacultural Science, 35, 2794-2800.
	[欧阳青, 任健, 尹俊, 李永进, 袁福锦 (2018). 短期增温对亚高山草甸土壤养分和脲酶的影响. 草业科学, 35, 2794-2800.]
[40]	Osterkamp TE (2004). Establishing long term permafrost observatories for active-layer and permafrost investigations in Alaska: 1977-2002. Permaf Rost & Peri Glacial Processes, 14, 331-342.
[41]	Poutou E, Krinner G, Genthon C, Noblet-Ducoudré N (2004). Role of soil freezing in future boreal climate change. Climate Dynamics, 23, 621-639.
[42]	Qin JH, Liu Q (2009). Impact of seasonally frozen soil on germinability and antioxidant enzyme activity of Picea asperata seeds. Canadian Journal of Forest Research, 39, 723-730.
[43]	Saiya-Cork KR, Sinsabaugh RL, Zak DR (2002). The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biology & Biochemistry, 34, 1309-1315.
[44]	Schmidt MWI, Torn MS, Abiven S, Dittmar T, Guggenberger G, Janssens IA, Kleber M, Kögel-Knabner I, Lehmann J, Manning DAC, Nannipieri P, Rasse DP, Weiner S, Trumbore SE (2011). Persistence of soil organic matter as an ecosystem property. Nature, 478, 49-56.
[45]	Song Y, Zou YC, Wang GP, Yu XF (2017). Altered soil carbon and nitrogen cycles due to the freeze-thaw effect: a meta-analysis. Soil Biology & Biochemistry, 109, 35-49.
[46]	Souza RC, Solly EF, Dawes MA, Graf F, Hagedorn F, Egli S, Clement CR, Nagy L, Rixen C, Peter M (2017). Responses of soil extracellular enzyme activities to experimental warming and CO₂enrichment at the alpine treeline. Plant and Soil, 416, 527-537.
[47]	Tan B, Wu FZ, Qin JL, Wu QG, Yang WQ (2014). Dynamics of soil microbial biomass and enzyme activity in the subalpine/alpine forests of western Sichuan. Ecology and Environmental Sciences, 23, 1265-1271.
	[谭波, 吴福忠, 秦嘉励, 吴庆贵, 杨万勤 (2014). 川西亚高山、高山森林土壤微生物生物量和酶活性动态特征. 生态环境学报, 23, 1265-1271.]
[48]	Tan B, Wu FZ, Yang WQ, Liu L, Yu S (2010). Characteristics of soil animal community in the subalpine/alpine forests of western Sichuan during onset of freezing. Acta Ecologica Sinica, 30, 93-99.
[49]	Tan B, Wu FZ, Yang WQ, Yu S, Liu L, Wang A (2011). The dynamics pattern of soil carbon and nutrients as soil thawing proceeded in the alpine/subalpine forest. Acta Agriculturae Scandinavica, Section B — Soil & Plant Science, 61, 670-679.
[50]	Ueda MU, Muller O, Nakamura M, Nakaji T, Hiura T (2013). Soil warming decreases inorganic and dissolved organic nitrogen pools by preventing the soil from freezing in a cool temperate forest. Soil Biology & Biochemistry, 61, 105-108.
[51]	Wang HY, Yang WQ (2012). Effects of seasonal freeze-thaw cycles on quantity of soil microbes in the subalpine fir forest. Scientia Silvae Sinicae, 48(5), 88-94.
	[王怀玉, 杨万勤 (2012). 季节性冻融对亚高山冷杉林土壤微生物数量的影响. 林业科学, 48(5), 88-94.]
[52]	Wang R, Zhu QK, Ma H, Ai N (2017). Spatial-temporal variations in near-surface soil freeze-thaw cycles in the source region of the Yellow River during the period 2002-2011 based on the Advanced Microwave Scanning Radiometer for the Earth Observing System (AMSR-E) data. Journal of Arid Land, 9, 850-864.
[53]	Wei WD, Liu YH, Ma H, Li JL (2018). Analysis of freeze-thaw action characteristics in shallow layer soil of degraded alpine steppe. Acta Agriculturae Boreali-Occidentalis Sinica, 27, 1358-1366.
	[魏卫东, 刘育红, 马辉, 李积兰 (2018). 退化高寒草原浅层土壤冻融作用特征分析. 西北农业学报, 27,1358-1366.]
[54]	Wu FZ, Yang WQ, Zhang J, Deng RJ (2010). Litter decomposition in two subalpine forests during the freeze-thaw season. Acta Oecologica, 36, 135-140.
[55]	Wu QB, Zhu YL, Shi B (2001). Study of frozen soil environment relating to engineering activities. Journal of Glaciology and Geocryology, 23, 200-207.
	[吴青柏, 朱元林, 施斌 (2001). 工程活动下的冻土环境研究. 冰川冻土, 23, 200-207.]
[56]	Xiong L, Xu ZF, Wu FZ, Yang WQ, Yin R, Li ZP, Gou XL, Tang SS (2014). Effects of snow pack on soil nitrogen transformation enzyme activities in a subalpine Abies faxoniana forest of western Sichuan, China. Chinese Journal of Applied Ecology, 25, 1293-1299.
	[熊莉, 徐振锋, 吴福忠, 杨万勤, 殷睿, 李志萍, 苟小林, 唐仕姗 (2014). 雪被斑块对川西亚高山冷杉林土壤氮转化酶活性的影响. 应用生态学报, 25, 1293-1299.]
[57]	Xue LX, Sun B, Yang YH, Jin B, Zhuang GQ, Bai ZH, Zhuang XL (2021). Efficiency and mechanism of reducing ammonia volatilization in alkaline farmland soil using Bacillus amyloliquefaciens biofertilizer. Environmental Research, 202, 111672. DOI: 10.1016/j.envres.2021.111672.
[58]	Yang WQ, Feng RF, Zhang J, Wang KY (2007). Carbon stock and biochemical properties in the organic layer and mineral soil under three subalpine forests in Western China. Acta Ecologica Sinica, 27, 4157-4165.
	[杨万勤, 冯瑞芳, 张健, 王开运 (2007). 中国西部3个亚高山森林土壤有机层和矿质层碳储量和生化特性. 生态学报, 27, 4157-4165.]
[59]	Zhang CJ, He JZ, Shen JP (2016). Global change field manipulative experiments and their applications in soil microbial ecology. Chinese Journal of Applied Ecology, 27, 1663-1673.
	[张翠景, 贺纪正, 沈菊培 (2016). 全球变化野外控制试验及其在土壤微生物生态学研究中的应用. 应用生态学报, 27, 1663-1673.]
[60]	Zhang JF, Xu RP, Liu Y, Zhang HM, Qin XR, Chen M (2021). Experimental study on mechanical properties of grouted fractured rock mass under freeze-thaw cycle. Journal of Experimental Mechanics, 36, 378-388.
	[张嘉凡, 徐荣平, 刘洋, 张慧梅, 覃祥瑞, 陈敏 (2021). 冻融循环作用下注浆裂隙岩体力学特性试验研究. 实验力学, 36, 378-388.]
[61]	Zhao CZ, Liu Q (2009). Growth and physiological responses of Picea asperata seedlings to elevated temperature and to nitrogen fertilization. Acta Physiologiae Plantarum, 31, 163-173.
[62]	Zhao CZ, Wang YJ, Zhang NN, Liang J, Li DD, Yin CY, Liu Q (2022). Seasonal shifts in the soil microbial community responded differently to in situ experimental warming in a natural forest and a plantation. European Journal of Soil Science, 73, e13135. DOI: 10.1111/ejss.13135.

冻融变化对西南亚高山森林优势种云杉和华西箭竹根区土壤理化性质与酶活性的影响

Effects of freeze-thaw changes on soil physicochemical properties and enzyme activities in root zone of Picea asperata and Fargesia nitida under subalpine forests of southwest China

全文

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 62

相关文章 15

编辑推荐

Metrics

本文评价

[1]	罗娜娜, 盛茂银, 王霖娇, 石庆龙, 何宇. 长期植被恢复对中国西南喀斯特石漠化土壤活性有机碳组分含量和酶活性的影响[J]. 植物生态学报, 2023, 47(6): 867-881.
[2]	冯可, 刘冬梅, 张琦, 安菁, 何双辉. 旅游干扰对松山油松林土壤微生物多样性及群落结构的影响[J]. 植物生态学报, 2023, 47(4): 584-596.
[3]	赵长兴, 赵维俊, 张兴林, 刘思敏, 牟文博, 刘金荣. 祁连山排露沟流域青海云杉种群种内竞争与促进作用分析[J]. 植物生态学报, 2022, 46(9): 1027-1037.
[4]	夏体泽, 李露双, 杨汉奇. 屏边空竹分布区海拔上下边界的土壤真菌群落特征[J]. 植物生态学报, 2022, 46(7): 823-833.
[5]	甘子莹, 王浩, 丁驰, 雷梅, 杨晓刚, 蔡敬琰, 丘清燕, 胡亚林. 亚热带森林不同植物及器官来源的可溶性有机质输入对土壤激发效应的影响及其作用机理[J]. 植物生态学报, 2022, 46(7): 797-810.
[6]	李东, 田秋香, 赵小祥, 林巧玲, 岳朋芸, 姜庆虎, 刘峰. 贡嘎山树线过渡带土壤胞外酶活性及其化学计量比特征[J]. 植物生态学报, 2022, 46(2): 232-242.
[7]	郝建锋, 周润惠, 姚小兰, 喻静, 陈聪琳, 向琳, 王姚瑶, 苏天成, 齐锦秋. 二代野猪放牧对夹金山针阔混交林物种多样性与土壤理化性质的影响[J]. 植物生态学报, 2022, 46(2): 197-207.
[8]	秦慧君, 焦亮, 周怡, 薛儒鸿, 柒常亮, 杜达石. 祁连山优势树木碳水化合物资源分配的海拔和树种效应[J]. 植物生态学报, 2022, 46(2): 208-219.
[9]	杜军, 王文, 何志斌, 陈龙飞, 蔺鹏飞, 朱喜, 田全彦. 祁连山青海云杉物候表型的空间分异及其内在机制[J]. 植物生态学报, 2021, 45(8): 834-843.
[10]	汪子微, 万松泽, 蒋洪毛, 胡扬, 马书琴, 陈有超, 鲁旭阳. 青藏高原不同高寒草地类型土壤酶活性及其影响因子[J]. 植物生态学报, 2021, 45(5): 528-538.
[11]	马书琴, 汪子微, 陈有超, 鲁旭阳. 藏北高寒草地土壤有机质化学组成对土壤蛋白酶和脲酶活性的影响[J]. 植物生态学报, 2021, 45(5): 516-527.
[12]	魏春雪, 杨璐, 汪金松, 杨家明, 史嘉炜, 田大栓, 周青平, 牛书丽. 实验增温对陆地生态系统根系生物量的影响[J]. 植物生态学报, 2021, 45(11): 1203-1212.
[13]	丁凯, 张毓婷, 张俊红, 柴雄, 周世水, 童再康. 不同密度杉木林对林下植被和土壤微生物群落结构的影响[J]. 植物生态学报, 2021, 45(1): 62-73.
[14]	胡宗达, 刘世荣, 罗明霞, 胡璟, 刘兴良, 李亚非, 余昊, 欧定华. 川西亚高山不同演替阶段天然次生林土壤碳氮含量及酶活性特征[J]. 植物生态学报, 2020, 44(9): 973-985.
[15]	罗林, 黄艳, 梁进, 汪恩涛, 胡君, 贺合亮, 赵春章. 西南亚高山针叶林主要树种互作及增温对根区土壤微生物群落的影响[J]. 植物生态学报, 2020, 44(8): 875-884.