四种牧草植物替代控制对黄顶菊入侵土壤细菌多样性的影响

doi:10.3724/SP.J.1258.2011.00045

摘要/Abstract

摘要：

土壤微生物群落与植物的生长发育密切相关, 入侵植物可以改变入侵地土壤微生物类群, 使土壤理化性质发生变化, 从而促进其入侵过程。该文通过比较高丹草(Sorghum bicolor × S. sudanense)、向日葵(Helianthus annuus)、紫花苜蓿(Medi- cago sativa)和多年生黑麦草(Lolium perenne)4种替代植物与黄顶菊(Flaveria bidentis)混合种植(以下简称混种)后不同时期的土壤细菌多样性的变化, 揭示土壤细菌群落对黄顶菊入侵及替代管理措施的响应规律。结果表明, 单独种植(以下简称单种)黄顶菊的土壤细菌多样性下降, 并且在整个生长期多样性指数多数情况下低于高丹草、向日葵、紫花苜蓿和多年生黑麦草单种或与黄顶菊混种的土壤。当4种替代植物与黄顶菊混种后, 土壤细菌16S rRNA的变性梯度凝胶电泳(DGGE)图谱与它们分别种植时存在明显差异, 且不同生长期各个混种土壤都有特征细菌群落。4种替代植物单种或与黄顶菊混种的土壤细菌Shannon多样性指数变化规律与植物生长发育趋势相同, 7月份达到高峰, 8月份开始降低。总之, 黄顶菊入侵降低了土壤细菌群落多样性, 4种替代植物与黄顶菊混种后, 又可提高土壤细菌群落多样性, 这种变化对黄顶菊成功入侵和替代防控具有重要作用。

关键词: 多样性, 黄顶菊, 入侵植物, 入侵生态学, 替代控制, 土壤细菌

Abstract:

Aims Invasive plants can alter soil physicochemical properties by changing the soil microbial community (which is closely related to plant growth and development) and thus further promote the invasion process. Our objectives were to (a) compare changes in soil bacterial diversity with cultivation of four replacement plants (Sorghum bicolor × S. sudanense, Helianthus annuus, Medicago sativa and Lolium perenne) mixed with Flaveria bidentis in different growth period and (b) determine the responses of the soil bacterial community to F. bidentis invasion and replacement.
Methods Total soil bacterial DNA was extracted by PowerSoil^® DNA Isolation Kit, 16S rRNA V3 fragments were amplified with bacterial universal primers, and purified fragments were cloned into pGEM-T-Easy vector. Sequence results were aligned on NCBI.
Important findings Soil bacterial diversity was decreased in F. bidentis monoculture and was lower than in the soils of the monocultures of the four replacement plants and in the soils of the replacement plants and F. bidentis. There were Hsignificant differencesH in soil bacterial 16S rRNA denaturing gradient gel electrophoresis (DGGE) fingerprinting patterns between the mixed- and monoculture soils. Also, there were characteristic bacterial communities with each mixed culture soil on different dates. The Shannon diversity index of soil bacteria peaked in July and decreased starting in August, and this change was synchronous with plant growth. Soil bacterial diversity was reduced following the invasion of F. bidentis, and that the diversity level was increased by cultivating replacement plants with F. bidentis. Therefore, we proposed that soil bacteria play an important role in F. bidentis invasion and control.

Key words: diversity, Flaveria bidentis, invasive plant, invasion ecology, replacement control, soil bacteria

闫素丽, 皇甫超河, 李刚, 左照江, 马杰, 杨殿林. 四种牧草植物替代控制对黄顶菊入侵土壤细菌多样性的影响. 植物生态学报, 2011, 35(1): 45-55. DOI: 10.3724/SP.J.1258.2011.00045

YAN Su-Li, HUANGFU Chao-He, LI Gang, ZUO Zhao-Jiang, MA Jie, YANG Dian-Lin. Effects of replacement control with four forage species on bacterial diversity of soil invaded by Flaveria bidentis. Chinese Journal of Plant Ecology, 2011, 35(1): 45-55. DOI: 10.3724/SP.J.1258.2011.00045

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2011.00045

https://www.plant-ecology.com/CN/Y2011/V35/I1/45

图/表 9

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割取条带位置 Band cut site		条带号 No. of the band	登陆号 Accession No. in GenBank	近缘菌群 Closest bacteria	相似度 Similarity (%)
6月 June	Fb	9 17	FJ152853.1 FJ785824.1	Uncultured bacterium clone TX5A_145 Uncultured bacterium clone EP9	100 94
7月 July	Fb	23	EF614061.1	Uncultured bacterium clone NGD40	99
8月 August	Fb	1	GQ410521.1	Uncultured Candidatus Amoebophilus sp.	97
9月 September	Fb	1 2 4 5 13 14	GQ860246.1 EF651068.1 DQ828045.1 AM936427.1 DQ642734.1 DQ444104.1	Uncultured bacterium clone AR141 Uncultured delta proteobacterium Uncultured proteobacterium Uncultured Rhodospirillaceae Uncultured soil bacterium Uncultured bacterium clone DS3-24	100 96 99 100 98 95

割取条带位置 Band cut site		条带号 No. of the band	登陆号 Accession No. in GenBank	近缘菌群 Closest bacteria	相似度 Similarity (%)
6月 June	Fb	9 17	FJ152853.1 FJ785824.1	Uncultured bacterium clone TX5A_145 Uncultured bacterium clone EP9	100 94
7月 July	Fb	23	EF614061.1	Uncultured bacterium clone NGD40	99
8月 August	Fb	1	GQ410521.1	Uncultured Candidatus Amoebophilus sp.	97
9月 September	Fb	1 2 4 5 13 14	GQ860246.1 EF651068.1 DQ828045.1 AM936427.1 DQ642734.1 DQ444104.1	Uncultured bacterium clone AR141 Uncultured delta proteobacterium Uncultured proteobacterium Uncultured Rhodospirillaceae Uncultured soil bacterium Uncultured bacterium clone DS3-24	100 96 99 100 98 95

割取条带位置 Band cut site		条带号 No. of the band	登陆号 Accession No. in GenBank	近缘菌群 Closest bacteria	相似度 Similarity (%)
6月 June	Ss	16	GU118711.1	Uncultured bacterium clone Mfra_M13	100
6月 June	Ss + Fb	11	FJ870384.1	Acidobacteria bacterium	100
7月 July	Ss	14 17 18	EU300452.1 EU834798.1 AY154495.1	Uncultured actinobacterium Uncultured bacterium clone OTUc49 Uncultured earthworm intestine bacterium	99 95 100
7月 July	Ss + Fb	3 4 8	AB531400.1 GQ500847.1 EF651444.1	Arthrobacter sp. Uncultured bacterium clone MACA- MR 18 Uncultured proteobacterium clone	100 100 94
8月 August	Ss	10 13	GU143709.1 AB451868.1	Rhizobium sp. MerE-11 Uncultured Firmicutes bacterium	100 100
8月 August	Ss + Fb	6 7 9	GU056305.1 FJ966196.1 FJ930738.1	Pseudoxanthomonas sp. Sphingobium sp. Uncultured bacterium clone W3-C09	98 98 98
9月 September	Ss	-	-	-	-
9月 September	Ss + Fb	-	-	-	-

割取条带位置 Band cut site		条带号 No. of the band	登陆号 Accession No. in GenBank	近缘菌群 Closest bacteria	相似度 Similarity (%)
6月 June	Ss	16	GU118711.1	Uncultured bacterium clone Mfra_M13	100
6月 June	Ss + Fb	11	FJ870384.1	Acidobacteria bacterium	100
7月 July	Ss	14 17 18	EU300452.1 EU834798.1 AY154495.1	Uncultured actinobacterium Uncultured bacterium clone OTUc49 Uncultured earthworm intestine bacterium	99 95 100
7月 July	Ss + Fb	3 4 8	AB531400.1 GQ500847.1 EF651444.1	Arthrobacter sp. Uncultured bacterium clone MACA- MR 18 Uncultured proteobacterium clone	100 100 94
8月 August	Ss	10 13	GU143709.1 AB451868.1	Rhizobium sp. MerE-11 Uncultured Firmicutes bacterium	100 100
8月 August	Ss + Fb	6 7 9	GU056305.1 FJ966196.1 FJ930738.1	Pseudoxanthomonas sp. Sphingobium sp. Uncultured bacterium clone W3-C09	98 98 98
9月 September	Ss	-	-	-	-
9月 September	Ss + Fb	-	-	-	-

割取条带位置 Band cut site		条带号 No. of the band	登陆号 Accession No. in GenBank	近缘菌群 Closest bacteria	相似度 Similarity (%)
6月 June	Ha	1 2 3 7	GQ487891.1 GU117236.1 GQ500847.1 EU122663.1	Uncultured bacterium clone T1-11 Uncultured bacterium clone Feb08_07C Uncultured bacterium clone MACA-MR18 Uncultured Acidobacteria bacterium	100 100 99 100
6月 June	Ha + Fb	-	-	-	-
7月 July	Ha	5 6 9 10 13 20	EF221451.1 FJ478699.1 EU849335.1 GQ495519.1 FJ946319.1 EU665105.1	Uncultured Actinobacterium clone SI-1M_C07 Uncultured bacterium clone p9c21ok Uncultured actinobacterium Uncultured bacterium clone VR-12-13 Uncultured alpha proteobacterium Uncultured Gemmatimonadales	100 99 98 99 100 98
7月 July	Ha + Fb	1 7 12 16	AB196109.1 FJ184006.1 GQ468625.1 GQ067089.1	Uncultured bacterium gene Uncultured alpha proteobacterium Uncultured Dactylosporangium sp. Uncultured bacterium clone nbw1121h01c1	95 100 100 98
8月 August	Ha	2 3 8 15	AJ538059.1 FJ949425.1 GU201109.1 EU810962.1	Uncultured Acidobacterium sp. Uncultured Chloroflexi bacterium Uncultured bacterium clone 6123Rh Uncultured actinobacterium	94 95 100 100
8月 August	Ha + Fb	14 16	GU122957.1 AY792291.1	Agrobacterium sp. DBT2TW2 Uncultured alpha proteobacterium	100 98
9月 September	Ha	10 11 16	GQ859734.1 GU200785.1 GQ994692.1	Uncultured bacterium clone AA145 Uncultured bacterium clone 1074Rh Uncultured organism clone anti_mic10	100 98 100
9月 September	Ha + Fb	6 7	EU300503.1 EU300495.1	Uncultured Firmicutes bacterium Uncultured Chloroflexi bacterium	100 98