植物生态学报 ›› 2024, Vol. 48 ›› Issue (3): 377-389.DOI: 10.17521/cjpe.2023.0172

• 研究论文 • 上一篇    下一篇

上海大金山岛不同植被类型土壤细菌群落的变异

杨安娜1, 李曾燕1, 牟凌1, 杨柏钰1, 赛碧乐1, 张立1, 张增可1, 王万胜2, 杜运才2, 由文辉1, 阎恩荣1,*()   

  1. 1华东师范大学生态与环境科学学院, 上海 200241
    2上海市金山区海洋海塘管理所, 上海 201508
  • 收稿日期:2023-06-14 接受日期:2023-10-09 出版日期:2024-03-20 发布日期:2024-04-24
  • 通讯作者: 阎恩荣
  • 基金资助:
    国家自然科学基金(32030068)

Variation in soil bacterial community across vegetation types in Dajinshan Island, Shanghai

YANG An-Na1, LI Zeng-Yan1, MOU Ling1, YANG Bai-Yu1, SAI Bi-Le1, ZHANG Li1, ZHANG Zeng-Ke1, WANG Wan-Sheng2, DU Yun-Cai2, YOU Wen-Hui1, YAN En-Rong1,*()   

  1. 1School of Ecological and Environmental Sciences, East China Normal University, Shanghai 200241, China
    2Jinshan Ocean and Coast Management Institute, Shanghai 201508, China
  • Received:2023-06-14 Accepted:2023-10-09 Online:2024-03-20 Published:2024-04-24
  • Contact: YAN En-Rong
  • Supported by:
    National Natural Science Foundation of China(32030068)

摘要:

揭示次生演替过程中土壤细菌群落对植被类型变化的响应格局, 有助于深化对生态系统地上地下相互作用机制的理解。该研究以上海大金山岛处于演替前、中和后期的落叶灌丛、落叶阔叶林和常绿阔叶林为对象, 通过测定土壤碳、氮、磷含量以及土壤细菌群落特征, 分析土壤细菌多样性、类群关系网络结构和标志性类群如何随植被演替而更替。结果发现: 土壤养分含量在常绿阔叶林显著高于落叶阔叶林, 但土壤细菌多样性在落叶阔叶林显著高于常绿阔叶林, 土壤养分含量和细菌多样性在落叶灌丛处于中等水平。土壤细菌的相关性网络节点、密度和复杂性在落叶阔叶林最高, 在落叶灌丛中等, 在常绿阔叶林最低。落叶灌丛和落叶阔叶林的优势土壤细菌分别为根瘤菌目(Rhizobiales)和伯克氏菌目(Burkholderiales)等具有潜在固氮功能的类群, 常绿阔叶林的优势土壤细菌为黄单胞菌目(Xanthomonadales)和嗜热芽菌目(Thermogemmatisporales)等具有潜在致病和抗病性功能的类群以及酸杆菌目(Acidobacteriales)等与纤维素降解相关的类群。该结果表明: 海岛植被演替过程中, 植物种类组成和土壤养分供给性的改变会极大地重塑土壤细菌多样性、群落组成、互作网络结构和标志类群。海岛常绿阔叶林显著更低的土壤细菌多样性、趋于松散简化的细菌网络结构以及具有潜在致病和抗病功能的标志类群的出现, 说明了顶极群落地下部分对地上部分退化趋势的响应。

关键词: 次生演替, 岛屿, 灌丛, 森林, 细菌多样性, 16S rDNA

Abstract:

Aims Revealing the response patterns of soil bacterial community to changes in vegetation type during secondary succession can improve our understanding of the mechanisms that structure the above- and below-ground interactions in ecosystems.

Methods To investigate how soil bacterial diversity, taxa network structure and biomarkers shift with vegetation succession, this study measured soil carbon, nitrogen and phosphorus contents, as well as soil bacterial community properties across shrubland, deciduous broadleaf forest and evergreen broadleaf forests, representing the early-, middle- and late-successional stages, respectively, on Dajinshan Island, Shanghai.

Important findings Soil nutrient contents were significantly higher in evergreen broadleaf forest than in deciduous broadleaf forest. However, soil bacterial diversity was significantly higher in deciduous broadleaf forest than in evergreen broadleaf forest, while soil nutrient content and bacterial diversity were medium in deciduous shrubland. The correlation network nodes, density and complexity of soil bacteria were highest in deciduous broadleaf forest, medium in deciduous shrubland, and lowest in evergreen broadleaf forest. The dominant soil bacteria in deciduous shrubland and broadleaf forest was Rhizobiales and Burkholderiales, respectively, which belong to functional group of nitrogen-fixing. The dominant soil bacterial in evergreen broadleaf forest were characterized by functional groups of pathogenicity and resistance such as Xanthomonadales and Thermogemmatisporales, and functional group associated with cellulose degradation such as Acidobacteriales. These results suggest that changes in plant species composition and soil nutrient availability during island vegetation succession can greatly reshape species diversity, community composition, interactive network structure and biomarkers of soil bacteria. In evergreen broadleaf forest, lowered soil bacterial diversity, simplified bacterial network structure, and emerged biomarkers of functional groups of pathogenicity and resistance suggest a response of belowground to the degraded trend of aboveground in the studied climax forest.

Key words: secondary succession, island, shrubland, forest, bacterial diversity, 16S rDNA