植物生态学报 ›› 2011, Vol. 35 ›› Issue (2): 223-231.DOI: 10.3724/SP.J.1258.2011.00223
王庆海1,*(), 张威1,2, 却晓娥3, 武菊英1, 张国安2, 肖波1
收稿日期:
2010-03-22
接受日期:
2010-11-16
出版日期:
2011-03-22
发布日期:
2011-01-21
作者简介:
王庆海, E-mail: qinghaiw@sina.com
WANG Qing-Hai1,*(), ZHANG Wei1,2, QUE Xiao-E3, WU Ju-Ying1, ZHANG Guo-An2, XIAO Bo1
Received:
2010-03-22
Accepted:
2010-11-16
Online:
2011-03-22
Published:
2011-01-21
摘要:
利用营养液水培法, 研究了5个阿特拉津(atrazine)浓度(1、2、4、8和16 mg·L-1)下水葱(Scirpus tabernaemontani)鲜重、叶片相对含水量(RWC)、丙二醛(MDA)含量、过氧化物酶(POD)活性、根系活力和叶绿素含量等的变化。结果表明, 水葱在阿特拉津胁迫下, 鲜重、RWC、叶绿素含量均有不同程度的下降, 根系活力和POD活性降低, 同时MDA含量上升, 膜脂过氧化程度加剧。由于阿特拉津的降解, 这种不良影响随处理时间的延长而减弱。但其对叶绿素含量的影响具持久性; 培养60天内, 叶绿素含量仍显著低于正常水平。阿特拉津浓度越高, 对水葱的植物毒性越高, 当浓度高于8 mg·L-1时, 水葱的生长和生理活动受到显著影响(p < 0.05); 低于1 mg·L-1时, 与对照无显著差异。
王庆海, 张威, 却晓娥, 武菊英, 张国安, 肖波. 水体阿特拉津残留对水葱生物量及生理特性的影响. 植物生态学报, 2011, 35(2): 223-231. DOI: 10.3724/SP.J.1258.2011.00223
WANG Qing-Hai, ZHANG Wei, QUE Xiao-E, WU Ju-Ying, ZHANG Guo-An, XIAO Bo. Effects of atrazine residue in water on biomass and physiological characteristics of Scirpus tabernaemontani . Chinese Journal of Plant Ecology, 2011, 35(2): 223-231. DOI: 10.3724/SP.J.1258.2011.00223
图1 不同培养时间阿特拉津对水葱鲜重增量的影响(平均值±标准偏差)。图中同一培养时间相同字母表示Duncan’s检验在0.05水平上差异不显著。
Fig. 1 Effects of atrazine on increment of fresh weight of Scirpus tabernaemontani different cultivation time (mean ± SD). Same letters in same cultivation time mean no significant difference by Duncan’s new multiple range test among treatments at the 0.05 level.
初始浓度 Initial atrazine concentration (mg·L-1) | 浓度变化 Change of atrazine concentration (mg·L-1) | |||
---|---|---|---|---|
15 days | 30 days | 45 days | 60 days | |
1 | 0.49 | 0.29 | 0.17 | 0.10 |
2 | 1.01 | 0.57 | 0.32 | 0.18 |
4 | 1.63 | 0.84 | 0.43 | 0.22 |
8 | 2.52 | 1.14 | 0.52 | 0.24 |
16 | 7.58 | 3.29 | 1.43 | 0.62 |
表1 实验期间培养液中阿特拉津浓度的变化
Table 1 Change of atrazine concentration in culture solution in experiment period
初始浓度 Initial atrazine concentration (mg·L-1) | 浓度变化 Change of atrazine concentration (mg·L-1) | |||
---|---|---|---|---|
15 days | 30 days | 45 days | 60 days | |
1 | 0.49 | 0.29 | 0.17 | 0.10 |
2 | 1.01 | 0.57 | 0.32 | 0.18 |
4 | 1.63 | 0.84 | 0.43 | 0.22 |
8 | 2.52 | 1.14 | 0.52 | 0.24 |
16 | 7.58 | 3.29 | 1.43 | 0.62 |
图2 不同培养时间阿特拉津对水葱根系活力的影响(平均值±标准偏差)。图注同图1。
Fig. 2 Effects of atrazine on the root activity of Scripus tabernaemontani in different cultivation time (mean ± SD). Notes see Fig. 1.
图3 阿特拉津对水葱叶片相对含水量的影响(平均值±标准偏差)。图注同图1。
Fig. 3 Effects of atrazine on relative water content (RWC) of leaf of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.
图4 阿特拉津对水葱过氧化物酶(POD)活性的影响(平均值±标准偏差)。图注同图1。
Fig. 4 Effects of atrazine on peroxidase (POD) activity of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.
图5 阿特拉津对水葱丙二醛含量的影响(平均值±标准偏差)。图注同图1。
Fig. 5 Effects of atrazine on malonaldehyde (MDA) content of Scripus tabernaemontani (mean ± SD). Notes see Fig. 1.
生理指标 Physiological indexes (y) | 回归方程 Equation | R2 | P > F |
---|---|---|---|
鲜重 Fresh weight (g) | y = -0.1927x + 18.7870 | 0.855 5 | 0.008 2 |
叶片相对含水量 RWC of leaves (%) | y = -1.2056x + 65.9110 | 0.774 2 | 0.020 8 |
根系活力 Root activity (μg·h-1·g-1) | y = -2.5399x + 81.2620 | 0.969 5 | 0.000 4 |
叶绿素含量 Chlorophyll a + b content (mg·g-1 DW) | y = -0.0240x + 0.4401 | 0.616 7 | 0.064 2 |
POD活性 POD activity (Δ470·min-1·μg-1·FW) | y = -0.3644x + 8.0227 | 0.982 1 | 0.000 1 |
MDA含量 MDA content (μm·g-1) | y = 0.0040x + 0.0790 | 0.672 3 | 0.045 7 |
表2 阿特拉津浓度(x)与水葱生理指标(y)回归方程
Table 2 The interrelation among the physiological indexes (y) of Scirpus tabernaemontani and atrazine concentration (x)
生理指标 Physiological indexes (y) | 回归方程 Equation | R2 | P > F |
---|---|---|---|
鲜重 Fresh weight (g) | y = -0.1927x + 18.7870 | 0.855 5 | 0.008 2 |
叶片相对含水量 RWC of leaves (%) | y = -1.2056x + 65.9110 | 0.774 2 | 0.020 8 |
根系活力 Root activity (μg·h-1·g-1) | y = -2.5399x + 81.2620 | 0.969 5 | 0.000 4 |
叶绿素含量 Chlorophyll a + b content (mg·g-1 DW) | y = -0.0240x + 0.4401 | 0.616 7 | 0.064 2 |
POD活性 POD activity (Δ470·min-1·μg-1·FW) | y = -0.3644x + 8.0227 | 0.982 1 | 0.000 1 |
MDA含量 MDA content (μm·g-1) | y = 0.0040x + 0.0790 | 0.672 3 | 0.045 7 |
[1] | Chen LC (陈龙池), Liao LP (廖利平), Wang SL (汪思龙) (2003). Effect of vanillin on nutrient absorbency of Chinese fir seedlings. Acta Phytoecologica Sinica (植物生态学报), 27, 41-46. (in Chinese with English abstract) |
[2] | Chen SY (陈少裕) (1991). Injury of membrane lipid peroxidation to plant cell. Plant Physiology Communications (植物生理学通讯), 27, 84-90. (in Chinese with English abstract) |
[3] | Correll DL, Wu TL (1982). Atrazine toxicity to submersed vascular plants in simulated estuarine microcosms. Aquatic Botany, 14, 151-158. |
[4] | Detenbeck NE, Hermanutz R, Allen K, Swift MC (1996). Fate and effects of the herbicide atrazine in flow-through wetland mesocosms. Environmental Toxicology and Chemistry, 15, 937-946. |
[5] | Fairchild JF, La Point TW, Schwartz TR (1994). Effects of an herbicide and insecticide mixture in aquatic mesocosms. Archives of Environment Contamination and Toxicology, 27, 527-533. |
[6] |
Graymore M, Stagnitti F, Allinson G (2001). Impacts of atrazine in aquatic ecosystems. Environment International, 26, 483-495.
DOI URL PMID |
[7] | Huber W (1993). Ecotoxicological relevance of atrazine in aquatic systems. Environmental Toxicology and Chemistry, 12, 1865-1881. |
[8] | Hu J (胡江), Dai XZ (代先祝), Li SP (李顺鹏) (2005). Effects of atrazine and its degrader Exiguobaterium sp. BTAH1 on soil microbial community. Chinese Journal of Applied Ecology (应用生态学报), 16, 1518-1522. (in Chinese with English abstract) |
[9] | Jiang XY (姜孝玉), Mei XY (梅星元), Yuan JL (袁均林), He HW (贺红武) (1998). Effects of 2,4-D on the activities of peroxidase and superoxide dismutase and the isoenzymes in rice seedlings. Journal of Central China Normal University (Natural Sciences) (华中师范大学学报(自然科学版)), 32, 199-202. (in Chinese with English abstract) |
[10] | Ji WD (计汪栋), Shi GX (施国新), Yang HY (杨海燕), Xu QS (徐勤松), Xu Y (许晔), Zhang H (张慧) (2007). Effects of Cu2+ stress on leaf physiological indice and ultrastructure of Potamogeton malaianus. Chinese Journal of Applied Ecology (应用生态学报), 18, 2727-2732. (in Chinese with English abstract) |
[11] |
Lakshminarayana JS, O’Neill HJ, Jonnavithula SD, Leger DA, Milburn PH (1992). Impact of atrazine-bearing agricultural tile drainage discharge on planktonic drift of a natural stream. Environmental Pollution, 76, 201-210.
DOI URL PMID |
[12] | Li HS (李合生) ( 2000). Principles and Techniques of Plant Physiological Biochemical Experiment (植物生理生化实验原理和技术). Higher Education Press, Beijing. (in Chinese) |
[13] | Li HY (李宏园), Ma H (马红), Tao B (陶波) (2006). Ecological risk assessment of atrazine and control strategy. Journal of Northeast Agricultural University (东北农业大学学报), 37, 552-556. (in Chinese with English abstract) |
[14] | Lin KF (林匡飞), Xu XQ (徐小清), Jin X (金霞), Ni HY (倪海燕), Xiang YL (项雅玲) (2005). Eco-toxicological effects of germanium stress on rice (Oryza sativa L.) and their critical value. Acta Ecologica Sinica (生态学报), 25, 108-114. (in Chinese with English abstract) |
[15] | Liu JK (刘建康) (1999). Advanced Aquatic Biology (高级水生生物学). Science Press, Beijing. (in Chinese) |
[16] | Liu WG (刘文革), Wang M (王鸣), Yan ZH (阎志红), Zhao H (赵红) (2004). Effect of cold hardening on SOD, POD activities and on contents of MDA in different ploidy watermelon seedling. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 24, 578-582. (in Chinese with English abstract) |
[17] | Liu ZL (刘周莉), He XY (何兴元), Chen W (陈玮) (2009). Effects of cadmium stress on the growth and physiological characteristics of Lonicera japonica. Chinese Journal of Applied Ecology (应用生态学报), 20, 40-44. (in Chinese with English abstract) |
[18] | Ren J (任晋), Jiang K (蒋可), Zhou HD (周怀东) (2002). The concentration and source of atrazine residue in water of Guanting Reservoir. Chinese Journal of Environmental Science (环境科学), 23, 126-128. (in Chinese with English abstract) |
[19] | Shen H (沈宏), Wang XC (王效常), Cao ZH (曹志洪) (1999). Study of inhibition of CuSO4 on moss growth. Agro-Environmental Protection (农业环境保护), 18(2), 57-58. (in Chinese) |
[20] | Shen WQ (沈伟其) (1988). Extraction of mixed solution for determination of chlorophyll content in rice leaf blade. Plant Physiology Communications (植物生理学通讯), ( 3), 62-64. (in Chinese with English abstract) |
[21] | Solomon KR, Baker DB, Richards RP, Klaine SJ, La Point TW, Kendall RJ, Weisskopf CP, Giddings JM, Giesy JP (1996). Ecological risk assessment of atrazine in North American surface waters. Environmental Toxicology and Chemistry, 15, 31-76. |
[22] | Su JW (苏金为), Wang XP (王湘平) (2002). Cadmium- induced membrane lipid peroxidation and programmed cell death in tea seedling. Journal of Plant Physiology and Molecular Biology (植物生理与分子生物学学报), 28, 292-298. (in Chinese with English abstract) |
[23] | Tang CC (唐除痴), Li YC (李煜昶), Chen B (陈彬) (1998). Pesticide Chemistry (农药化学). Nankai University Press, Tianjin. (in Chinese) |
[24] | Tang LZ (唐罗忠), Cheng SW (程淑婉), Xu XZ (徐锡增), Li YQ (李跃青) (1999). Effects of waterlogging stress on leaf growth and physiological properties of poplar clone seedlings. Journal of Plant Resources and Environment (植物资源与环境), 8(1), 15-21. (in Chinese with English abstract) |
[25] | Wacksman MN, Maul JD, Lydy MJ (2006). Impact of atrazine on chlorpyrifos toxicity in four aquatic vertebrates. Archives of Environmental Contamination and Toxicology, 4, 681-689. |
[26] | Wan NS (万年升), Gu JD (顾继东), Duan SS (段舜山) (2006). Eco-toxicity and biodegradation of atrazine in the environment. Acta Scientiae Cireumstantia (环境科学学报), 26, 552-560. (in Chinese with English abstract) |
[27] | Winkelmann DA, Klaine SJ (1991). Degradation and bound residue formation of four atrazine metabolites, deethylatrazine, deisopropylatrazine, dealkylatrazine and hydro- xyatrazine, in a western Tennessee soil. Environmental Toxicology and Chemistry, 10, 347-354. |
[28] | Wu YJ (武永军), He GQ (何国强), Shi YR (史艳茹), Liang ZS (梁宗锁) (2009). Change of relative water content, proline content and malondialdehyde content of Vicia faba leaves under different pH buffer treatments. Agricultural Research in the Arid Areas (干旱地区农业研究), 27, 169-172. (in Chinese with English abstract) |
[29] | Wang CY (王长义), Guo SR (郭世荣), Liu CJ (刘超杰) (2009). Effects of calcium on expression of defense enzymes isoenzymes in roots of cucumber seedlings under root-zone hypoxic stress. Acta Botanica Boreali-Occiden- lia Sinica (西北植物学报), 29, 1874-1880. (in Chinese with English abstract) |
[30] | Wang ZY (王振英), Zheng JH (郑津辉), Jin HL (金慧玲), Peng YK (彭永康) (2001). Effect of phosphoric amide herbicide APM on POD and protein compositions of Hordeum vulgare and Secale cereale. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 21, 662-667. (in Chinese with English abstract) |
[31] | Xu QM (徐秋曼), Wang XL (王修鲁), Cheng JS (程景胜) (2008). Effects of Ce(NH4)2(NO3)6 on seed generation and physiological characteristics of rice. Journal of Agro- Environment Science (农业环境科学学报), 27, 442-446. (in Chinese with English abstract) |
[32] | Ye XQ (叶新强), Lu Y (鲁岩), Zhang H (张恒) (2006). The usage and permiciousness of the herbicide atrazine. Environmental Science and Management (环境科学与管理), 31(8), 95-97. (in Chinese with English abstract) |
[33] | Zou Q (邹琦) (2000). The Experimental Guider for Plant Physiology (植物生理学实验指导). China Agriculture Press, Beijing. (in Chinese) |
[1] | 王袼, 胡姝娅, 李阳, 陈晓鹏, 李红玉, 董宽虎, 何念鹏, 王常慧. 不同类型草原土壤净氮矿化速率的温度敏感性[J]. 植物生态学报, 2024, 48(4): 523-533. |
[2] | 梁逸娴, 王传宽, 臧妙涵, 上官虹玉, 刘逸潇, 全先奎. 落叶松径向生长和生物量分配对气候变暖的响应[J]. 植物生态学报, 2024, 48(4): 459-468. |
[3] | 黄玲, 王榛, 马泽, 杨发林, 李岚, SEREKPAYEV Nurlan, NOGAYEV Adilbek, 侯扶江. 长期放牧和氮添加对黄土高原典型草原长芒草种群生长的影响[J]. 植物生态学报, 2024, 48(3): 317-330. |
[4] | 耿雪琪, 唐亚坤, 王丽娜, 邓旭, 张泽凌, 周莹. 氮添加增加中国陆生植物生物量并降低其氮利用效率[J]. 植物生态学报, 2024, 48(2): 147-157. |
[5] | 李娜, 唐士明, 郭建英, 田茹, 王姗, 胡冰, 罗永红, 徐柱文. 放牧对内蒙古草地植物群落特征影响的meta分析[J]. 植物生态学报, 2023, 47(9): 1256-1269. |
[6] | 赵艳超, 陈立同. 土壤养分对青藏高原高寒草地生物量响应增温的调节作用[J]. 植物生态学报, 2023, 47(8): 1071-1081. |
[7] | 苏炜, 陈平, 吴婷, 刘岳, 宋雨婷, 刘旭军, 刘菊秀. 氮添加与干季延长对降香黄檀幼苗非结构性碳水化合物、养分与生物量的影响[J]. 植物生态学报, 2023, 47(8): 1094-1104. |
[8] | 李冠军, 陈珑, 余雯静, 苏亲桂, 吴承祯, 苏军, 李键. 固体培养内生真菌对土壤盐胁迫下木麻黄幼苗渗透调节和抗氧化系统的影响[J]. 植物生态学报, 2023, 47(6): 804-821. |
[9] | 罗娜娜, 盛茂银, 王霖娇, 石庆龙, 何宇. 长期植被恢复对中国西南喀斯特石漠化土壤活性有机碳组分含量和酶活性的影响[J]. 植物生态学报, 2023, 47(6): 867-881. |
[10] | 杜英东, 袁相洋, 冯兆忠. 不同形态氮对杨树光合特性及生长的影响[J]. 植物生态学报, 2023, 47(3): 348-360. |
[11] | 和璐璐, 张萱, 章毓文, 王晓霞, 刘亚栋, 刘岩, 范子莹, 何远洋, 席本野, 段劼. 辽东山区不同坡向长白落叶松人工林树冠特征与林木生长关系[J]. 植物生态学报, 2023, 47(11): 1523-1539. |
[12] | 刘艳杰, 刘玉龙, 王传宽, 王兴昌. 东北温带森林5个羽状复叶树种叶成本-效益关系比较[J]. 植物生态学报, 2023, 47(11): 1540-1550. |
[13] | 郝晴, 黄昌. 森林地上生物量遥感估算研究综述[J]. 植物生态学报, 2023, 47(10): 1356-1374. |
[14] | 李变变, 张凤华, 赵亚光, 孙秉楠. 不同刈割程度对油莎豆非结构性碳水化合物代谢及生物量的影响[J]. 植物生态学报, 2023, 47(1): 101-113. |
[15] | 袁春阳, 李济宏, 韩鑫, 洪宗文, 刘宣, 杜婷, 游成铭, 李晗, 谭波, 徐振锋. 树种对土壤微生物生物量碳氮的影响: 同质园实验[J]. 植物生态学报, 2022, 46(8): 882-889. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||
Copyright © 2022 版权所有 《植物生态学报》编辑部
地址: 北京香山南辛村20号, 邮编: 100093
Tel.: 010-62836134, 62836138; Fax: 010-82599431; E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn
备案号: 京ICP备16067583号-19