环境胁迫对雷公藤中雷公藤红素含量的影响

doi:10.3773/j.issn.1005-264x.2009.01.020

摘要/Abstract

摘要：

雷公藤(Tripterygium wilfordii)是我国的名贵药用植物, 其药用成分雷公藤红素是医药研究中重要的化学单体。环境胁迫能促进药用植物次生代谢物质的合成与积累, 该文通过正交设计, 探讨了水分、光照、土壤氮含量三因素两水平轻度胁迫处理对雷公藤幼苗中雷公藤红素含量的影响。研究结果表明: 根部是雷公藤红素含量最丰富的部位; 雷公藤幼苗在水分、光照及土壤氮含量胁迫条件下根系中雷公藤红素的含量分别提高了10.8％、14.4％和13.0％, 光照对雷公藤红素含量的增加最明显, 短期的轻度胁迫可提高根中雷公藤红素的含量。

关键词: 雷公藤, 胁迫, 雷公藤红素, 次生代谢

Abstract:

Aims Tripterygium wilfordii is a highly valuable traditional Chinese medicinal plant. Celastrol, an active compound of T. wilfordii, has recently received attention from pharmaceutical researchers due to its medicinal value. Environmental stress may promote the formation and accumulation of secondary metabolites of medicinal plants. Our objective was to investigate whether mild environmental stress increased the content of Celastrol in T. wilfordii.
Methods Orthogonal comparison of mild stress treatments of water, sunlight and N content were performed on the seedlings of T. wilfordii in the greenhouse. The content of Celastrol was determined by High Performance Liquid Chromatography (HPLC).
Important findings Celastrol was mainly produced in the seedling root and was increased 10.8%, 14.4% and 13.0% under the three different stress treatments, respectively. Therefore, mild stress treatment improves the synthesis and accumulation of Celastrol.

Key words: Tripterygium wilfordii, environmental stress, Celastrol, secondary metabolism

杜玮炜, 姚小洪, 黄宏文. 环境胁迫对雷公藤中雷公藤红素含量的影响. 植物生态学报, 2009, 33(1): 180-185. DOI: 10.3773/j.issn.1005-264x.2009.01.020

DU Wei-Wei, YAO Xiao-Hong, HUANG Hong-Wen. EFFECTS OF ENVIRONMENTAL STRESSES ON CELASTROL CONTENTS IN TRIPTERYGIUM WILFORDII. Chinese Journal of Plant Ecology, 2009, 33(1): 180-185. DOI: 10.3773/j.issn.1005-264x.2009.01.020

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.01.020

https://www.plant-ecology.com/CN/Y2009/V33/I1/180

图/表 6

图1 雷公藤红素化学结构

Fig. 1 Chemical structure of Celastrol (Duan et al., 2000, 2001)

表1 不同胁迫处理对雷公藤红素含量影响的方差分析

Table 1 The Analysis of Variance (ANOVA) of between-subjects effects on content of Celastrol under different stress treatments

变异来源 Source	平方和 SS	自由度 df	样本均方 Mean square	F值 F	显著性 Sig.
模型 Model	2 116.893^a	8	264.612	2 111.155	0.000
l	9.445	1	9.445	46.304	0.000
r	6.875	1	6.875	33.704	0.000
n	7.746	1	7.746	37.974	0.000
l×r	1.482	1	1.482	11.822	0.002
l×n	0.127	1	0.127	1.014	0.321
r×n	1.475	1	1.475	11.769	0.002
l×r×n	0.248	1	0.248	1.979	0.169
误差 Error	4.011	32	0.125
总数 Total	2 120.904	40

图2 不同胁迫条件下雷公藤中雷公藤红素含量图 L: 光照正常 Full sunlight l: 光照胁迫 Light tress R: 水分正常 Normal water r: 水分胁迫 Water stress N: N含量正常 Normal N content n: N含量胁迫 N content stress l×r×n: 光照胁迫、水分胁迫与N含量胁迫互作 Interaction among sunlight stress, water stress and N content stress l×r: 光照胁迫与水分胁迫互作 Interaction between sunlight stress and water stress l×n: 光照胁迫与N含量胁迫互作 Interaction between sunlight stress and N content stress r×n: 水分胁迫与N含量胁迫互作 Interaction between water stress and N content stress LRN: 对照 Control 柱子上的小写字母表示各处理间差异显著性 Small letters on the pillars indicate whether there exist significant differences between treatments

Fig. 2 The content of Celastrol in Tripterygium wilfordii under different stress conditions

图3 水分胁迫对雷公藤红素含量的影响 LN: 光照正常, 氮含量正常 Full sunlight and normal N content Ln: 光照正常, 氮含量胁迫 Full sunlight and N content stress lN: 光照胁迫与氮含量正常 Sunlight stress and normal N content ln: 光照胁迫与氮含量胁迫 Sunlight stress and N content stress 柱子上的小写字母表示各处理间差异显著性 Small letters on the pillars indicate whether there exist significant differences between treatments

Fig. 3 Effects of water stress on Celastrol content in Tripterygium wilfordii

图4 光照胁迫对雷公藤红素含量的影响 RN: 水分与氮含量皆正常 Normal water and N content Rn: 水分正常氮含量胁迫 Normal water and N content stress rN: 水分胁迫与氮含量正常 Water stress and normal N content rn: 水分胁迫与氮含量胁迫 Water stress and N content stress 柱子上的小写字母表示各处理间差异显著性 Small letters on the pillars indicate whether there exist significant differences between treatments

Fig. 4 Effects of light stress on Celastrol content in Tripterygium wilfordii

图5 土壤N含量胁迫对雷公藤中雷公藤红素含量的影响 LR: 光照与水分皆正常 Full sunlight and normal water Lr: 光照正常与水分胁迫 Full sunlight and water stress lR: 光照胁迫与水分正常 Sunlight stress and normal water lr: 光照胁迫与水分胁迫 Sunlight stress and water stress 柱子上的小写字母表示各处理间差异显著性 Small letters on the pillars indicate whether there exist significant differences between treatments

Fig. 5 Effects of N content stress in soil on the content of Celastrol in Tripterygium wilfordii

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