不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系

doi:10.3773/j.issn.1005-264x.2009.03.017

植物生态学报 ›› 2009, Vol. 33 ›› Issue (3): 580-586.DOI: 10.3773/j.issn.1005-264x.2009.03.017

不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系

韦泽秀¹^,⁴, 梁银丽¹^,²^,^*(), 山田智³, 曾兴权⁴, 周茂娟¹, 黄茂林¹, 吴燕¹

1 西北农林科技大学资源环境学院,陕西杨凌 712100
2 中国科学院水利部水土保持研究所,陕西杨凌 712100
3 日本鸟取大学农学部,日本鸟取 680-8553
4 西藏自治区农牧科学院农业研究所,拉萨 850002

收稿日期:2008-10-09 接受日期:2009-01-06 出版日期:2009-10-09 发布日期:2009-05-31
通讯作者: 梁银丽
作者简介:*E-mail: liangyl@ms.iswc.ac.cn
基金资助:
中国科学院西部行动项目(KZCX2-XB2-05-01);中国科学院知识创新重要方向项目(KZCX2-YW-443-3);国家科技支撑项目(2006BAD09B07);中国科学院安塞站和中国科学院水土保持研究所领域前沿项目(SW04302)

RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS

WEI Ze-Xiu¹^,⁴, LIANG Yin-Li¹^,²^,^*(), YAMADA Satoshi³, ZENG Xing-Quan⁴, ZHOU Mao-Juan¹, HUANG Mao-Lin¹, WU Yan¹

¹College of Resource and Environment, Northwest Sci &Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China
²Institute of Water and Soil Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi 712100, China
³Faculty of Agriculture, Tottori University 4-101 Koyamach-Minama, 680-8553, Japan
⁴Tibet Agriculture Research Institute, Lhasa 850032, China

Received:2008-10-09 Accepted:2009-01-06 Online:2009-10-09 Published:2009-05-31
Contact: LIANG Yin-Li

摘要/Abstract

摘要：

采用内含子切接点引物 (Intron-splice junction primer) 和长随机引物的PCR (Polymerase chain reaction, 聚合酶链) 标记技术, 就黄土高原不同水肥处理对日光温室番茄土壤微生物群落多样性进行了研究, 并对产量、品质的影响进行了分析。结果表明, 所用的6个引物共能扩增出182条稳定清晰的条带, 其中142条为多态性条带, 多态性条带为78.02%。聚类分析与主坐标分析表明, 水肥对土壤微生物群落多样性的变化有不同的影响。土壤微生物群落多样性指数与番茄产量、果实的Vc、可溶性蛋白质含量成正相关, 尤其与Vc的相关系数达0.9211, 而与可溶性固形物含量成负相关关系。6个水肥组合中WmFh (中水高肥) 土壤微生物群落多样性指数最高, 且番茄果实的Vc、可溶性蛋白质含量及产量显著高于其他处理, 可溶性固形物含量较低。该组合有利于土壤微生物群落的多样性和稳定性的提高, 利于土壤生态环境的改善和番茄优良品质的形成。

关键词: 内含子切接点引物, 多样性指数, 产量, 品质

Abstract:

Aims Our objective was to investigate diversity within soil microbial communities and tomato yields and qualities under different soil water and fertilization treatments.

Methods PCR (polymerase chain reaction) with long random primers and intron-splice junction primers were used to study greenhouse soil microbial diversity with tomato cultivated under different treat-ments.

Important findings A total of 182 bands were detected with 6 primer combinations, and 142 (78.02%) of the bands were polymorphic. Soil microbial community diversity was affected by the different soil water contents and fertilizations. Diversity was positively correlated with yields, VC contents and solu-ble protein contents, but negatively correlated with soluble solids contents. The highest soil microbial community diversity index was detected under the treatment of medium water level and high fertilization; the VC contents, soluble protein contents and yields of tomato fruits were significantly higher and the soluble solids of the tomato fruits was lower than those in other treatments. This showed that treatment can enhance the soil microbial community diversity and stability and to improve soil ecological environments and tomato quality.

Key words: intron-splice junction primer, Shannon-Wiener index, yield, quality

韦泽秀, 梁银丽, 山田智, 曾兴权, 周茂娟, 黄茂林, 吴燕. 不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系. 植物生态学报, 2009, 33(3): 580-586. DOI: 10.3773/j.issn.1005-264x.2009.03.017

WEI Ze-Xiu, LIANG Yin-Li, YAMADA Satoshi, ZENG Xing-Quan, ZHOU Mao-Juan, HUANG Mao-Lin, WU Yan. RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS. Chinese Journal of Plant Ecology, 2009, 33(3): 580-586. DOI: 10.3773/j.issn.1005-264x.2009.03.017

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.03.017

https://www.plant-ecology.com/CN/Y2009/V33/I3/580

图/表 9

表1 日光温室内0~20 cm土壤化学特性

Table 1 The chemical properties of soil layer 0~20 cm in the greenhouse

图1 安塞站内日光温室4至10月平均温度和相对湿度变化

Fig.1 Mean temperature and RH from April to October in greenhouhouse at Ansai station

表2 引物序列及扩增结果

Table 2 The sequence of the primers and the result of amplified

图2 不同水肥处理PCR扩增结果聚类图 Wh Fh、Wm Fh、Wl Fh处理:土壤相对含水量分别为90%~100%, 70%~80%, 50%~60%, 施肥量600 kg·hm-2N和420 kg·hm-2P2O5 In Wh Fh, Wm Fh, Wl Fh treatments, soil relative water content were 90%-100%, 70%-80%, 50%-60%respectively and fertilizer amount were 600kg·hm-2N and 420 kg·hm-2P2O5;Wh Fl、Wm Fl、Wl Fl处理:土壤相对含水量分别为90%~100%, 70%~80%, 50%~60%, 施肥量420 kg·hm-2N和294 kg·hm-2P2O5;In Wh Fl, Wm Fl, Wl Fl treatments, soil relative water content were 90%-100%, 70%-80%, 50%-60%respectively and fertilizer amount were 420 kg·hm-2N and 294 kg·hm-2P2O5

Fig.2 The dendrogram of different water and fertiliza-tion treatment based on PCR pattern

图3 6个不同水肥处理的主坐标分析图注同图2

Fig.3 2D plots of principal coordinate analysis of six different treatment based on PCR data using NTSYS-pc program Note see Fig.2

图4 不同处理土壤微生物遗传多样性指数图注同图2

Fig.4 The soil microbial Shannon-wiener index of different treatments Note see Fig.2

图5 L1内含子切接点引物扩增图图注同图2

Fig.5 The amplification map of L1 intron-splice junction primer Note see Fig.2

表3 不同水肥处理的番茄产量与品质

Table 3 The yield and fruit nutrient quality in different treatments

表4 土壤微生物群落多样性指数与番茄产量、品质相关分析结果

Table 4 The correlation between soil microbial community diversity index with tomato yield and fruit quality

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不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系

RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS

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