外生菌根真菌对重金属铜镉污染土壤中油松生长和元素积累分布的影响

doi:10.17521/cjpe.2007.0117

摘要/Abstract

摘要：

该实验选取处于生长期的油松(Pinus tabulaeformis)幼苗,研究土壤Cu、Cd胁迫条件下,美味牛肝菌(Boletus edulis)单独接种、红绒盖牛肝菌(Xerocomus chrysenteron)与美味牛肝菌混合接种处理,对油松幼苗的生长和重金属积累分布状况的影响,探讨不同接种对油松抗性的影响。研究发现,菌根接种不仅促进寄主油松的生长发育和生物量积累,而且显著降低油松体内的重金属积累浓度,减少重金属由根部向植物茎叶部分的转运。与单一接种相比,混合接种可以更加有效地缓解重金属对寄主的生物毒性,减少土壤中重金属元素向油松体内的转运。这种优势在高浓度的重金属胁迫环境下尤为明显。该实验中,在3 mg·kg^-1 Cd胁迫下,混合菌根油松的茎叶和根部Cd浓度仅为未接种对照的59.1%和70.7%,比单一菌根降低了11.3%和18.1%,而混合菌根植物的茎叶和根部生物量干重则分别为未接种对照的1.14和1.20倍,单一菌根为未接种对照的1.18和1.17倍。在400 mg·kg^-1Cu胁迫下,混合菌根植物茎叶和根部的干重分别是未接种植株的1.01和1.09倍,而混合菌根植物茎叶和根部的Cu浓度仅为未接种植株的61.8%和79.6%,比Boletus edulis菌根植物的Cu积累浓度下降了0.7%和3.8%。

关键词: 外生菌根真菌, 油松, 铜, 镉, 积累

Abstract:

Aims Ectomycorrhiza (ECM) was generally considered to have a positive impact on the host plant exposed in contaminated soil and could enhance heavy metal tolerance. However, most researches have focused on single-ECM seedlings in experimental conditions, and the effects of mixed-ECM on the host are inadequately studied. Because woody plants are usually inoculated by mixed-ECM in natural conditions, it is essential to study the differences between single- and mixed-ECM inoculations, especially the dissimilar effects on heavy metal tolerance, which can help us better understand the mechanism of ectomycorrhiza.

Methods After a 4-week growth period, Pinus tabulaeformis seedlings inoculated by Boletus edulis and Xerocomus chrysenteron and the uninoculated control were planted in Cu and Cd contaminated soil. We measured growth and element accumulation 12 weeks later.

Important findings Compared with the uninoculated control, ECM inoculation not only enhanced the host's growth and biomass, but also reduced the concentration of heavy metals and their transportation from root to shoot. Seedlings inoculated by mixed-ECM strains had higher heavy metal tolerance than those inoculated by single-ECM strain. This advantage was especially remarkable at higher concentration. Results showed that in 3 mg·kg^-1 Cd contaminated soil, Cd concentrations in the shoot and root of mixed-ECM seedlings were only 59.1% and 70.7% of the control, 11.3% and 18.1% lower than single-ECM ones. However, the dry weights of mixed-ECM seedlings' shoot and root were 1.14 and 1.20 times than the control vs. 1.18 and 1.17 times for single-ECM. In 400 mg·kg^-1 Cu contaminated soil, the dry weights of mixed-ECM seedlings' shoot and root were 1.01 and 1.09 times than the control, while the Cu concentrations in the shoot and root of mixed-ECM seedlings were only 61.8% and 79.6% of the control, 0.7% and 3.8% lower than single-ECM seedlings.

Key words: ectomycorrhizal fungi, Pinus tabulaeformis, Cu, Cd, accumulation

黄艺, 彭博, 李婷, 梁振春. 外生菌根真菌对重金属铜镉污染土壤中油松生长和元素积累分布的影响. 植物生态学报, 2007, 31(5): 923-929. DOI: 10.17521/cjpe.2007.0117

HUANG Yi, PENG Bo, LI Ting, LIANG Zhen-Chun. GROWTH AND ELEMENT ACCUMULATION OF PINUS TABULAEFORMIS SEEDLINGS INFLUENCED BY INOCULATION OF ECTOMYCORRHIZAL FUNGI IN Cu AND Cd CONTAMINATED SOIL. Chinese Journal of Plant Ecology, 2007, 31(5): 923-929. DOI: 10.17521/cjpe.2007.0117

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https://www.plant-ecology.com/CN/Y2007/V31/I5/923

图/表 6

表1 供试土壤基本理化性质

Table 1 General soil characteristics of the soils used in the experiment

土壤类型 Soil type	pH值 pH value (1∶2.5)	总有机碳 Total organic carbon (%)	全氮 Total N (%)		全磷 Total P (%)		土壤中金属本底含量 Heavy metal concentrations in soil
土壤类型 Soil type	pH值 pH value (1∶2.5)	总有机碳 Total organic carbon (%)	全氮 Total N (%)		全磷 Total P (%)		Cu (mg·kg^-1)		Cd (mg·kg^-1)
褐土 Brown soil	7.5	1.668 3	0.093 8		0.267 6		25		0.064

图1 不同土壤Cu、Cd处理浓度下接种差异对油松生物量干重的影响 B.e: 美味牛肝菌 Boletus edulis X.c: 红绒盖牛肝菌 Xerocomus chrysenteron *和**分别表示接种处理与未接种处理之间在5%和1%的显著性水平下有差异 * and ** indicate 5% and 1% significant differences between inoculated and uninoculated ectomycorrhizal fungus respectively

Fig.1 Effect of ectomycorrhizal infection on the dry weight of biomass under different Cu and Cd treatments

图2 不同重金属胁迫条件下接种差异对油松根茎比的影响 B.e、X.c: 见图1 See Fig. 1

Fig.2 The root/shoot ratio of Pinus tabulaeformis seedling influenced by ectomycorrhizal infection under different heavy metal treatments

图3 不同Cu、Cd处理浓度下接种差异对油松根长的影响 B.e、X.c: 见图1 See Fig. 1

Fig.3 Effect of ectomycorrhizal infection on the root length under different Cu and Cd treatments

表2 Cu、Cd处理和接种差异对油松积累浓度的影响

Table 2 The effects of heavy metal treatments and inoculation on accumulated concentration in seedlings

重金属 Metal	部位 Section	变量 Source of variation	自由度 df	方差 MS	F值 F-value	p值 p-value
铜Cu	茎叶Shoot	铜Cu	2	0.000 04	1.80	0.180
		接种Infection status	3	0.000 14	7.12	0.001
		铜与接种Cu & infection	6	0.000 09	4.44	0.002
	根部Root	铜Cu	2	0.016 74	8.02	0.001
		接种Infection status	3	1.191 29	570.83	<0.001
		铜与接种Cu & infection	6	0.007 58	3.63	0.006
镉Cd	茎叶Shoot	镉Cd	2	0.019 12	21.95	<0.001
		接种Infection status	3	0.107 06	122.91	<0.001
		镉与接种Cd & infection	6	0.002 89	3.32	0.011
	根部Root	镉Cd	2	2.349 90	10.79	<0.001
		接种Infection status	3	54.125 08	248.49	<0.001
		镉与接种Cd & infection	6	0.550 28	2.53	0.038

图4 菌根接种对油松体内Cu、Cd积累浓度的影响图注见图1

Fig.4 Effect of ectomycorrhizal infection on the Cu and Cd accumulated concentration in seedlings Notes see Fig. 1

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