植物生态学报 ›› 2014, Vol. 38 ›› Issue (4): 375-386.DOI: 10.3724/SP.J.1258.2014.00034
所属专题: 青藏高原植物生态学:生理生态学
师生波1(), 张怀刚1, 师瑞2, 李妙1,3, 陈文杰1, 孙亚男1,3
收稿日期:
2013-11-28
接受日期:
2014-02-11
出版日期:
2014-11-28
发布日期:
2014-04-08
作者简介:
*E-mail:sbshi@nwipb.cas.cn
基金资助:
SHI Sheng-Bo1(), ZHANG Huai-Gang1, SHI Rui2, LI Miao1,3, CHEN Wen-Jie1, SUN Ya-Nan1,3
Received:
2013-11-28
Accepted:
2014-02-11
Online:
2014-11-28
Published:
2014-04-08
摘要:
在青海省都兰县香日德镇东盛村, 以中国科学院西北高原生物研究所培育的春小麦(Triticum aestivum)品种为材料, 主要采用调制叶绿素荧光分析手段, 研究了抽穗期旗叶光合作用的光抑制现象, 并分析了非光化学猝灭组分的光诱导和非光诱导耗散的量子产量变化。结果表明, 高原春小麦各品种间旗叶光合色素含量和比叶重存在差异; 全晴天3个典型时段准确暗适应20 min后的PSII最大光化学效率(Fv/Fm)的比较分析证实, 高原春小麦存在着光合作用的光抑制现象, Fv/Fm的降低是由于PSII反应中心的可逆失活; 稳态作用光下PSII有效光化学效率(Fv′/Fm′)易受持续强光胁迫的影响, 而PSII实际光化学效率(ΦPSII)在各春小麦品种间的差异略为明显; 上下午间4个春小麦品种的光化学猝灭系数(qP)和非光化学猝灭系数(NPQ)呈较一致的变化趋势, 显然qP和NPQ既属品种的内禀特性, 又与强太阳光胁迫的累积密切相关; 非光化学猝灭组分中光诱导的PSII调节性能量耗散的量子产量(ΦNPQ)所占比例较大, 下午时分ΦNPQ的上调反映了高原春小麦对青藏高原持续强光胁迫的驯化适应。
师生波, 张怀刚, 师瑞, 李妙, 陈文杰, 孙亚男. 青藏高原春小麦叶片光合作用的光抑制及PSII反应中心光化学效率的恢复分析. 植物生态学报, 2014, 38(4): 375-386. DOI: 10.3724/SP.J.1258.2014.00034
SHI Sheng-Bo, ZHANG Huai-Gang, SHI Rui, LI Miao, CHEN Wen-Jie, SUN Ya-Nan. Assessment of photosynthetic photo-inhibition and recovery of PSII photochemical efficiency in leaves of wheat varieties in Qinghai-Xizang Plateau. Chinese Journal of Plant Ecology, 2014, 38(4): 375-386. DOI: 10.3724/SP.J.1258.2014.00034
图1 抽穗期4个高原春小麦品种旗叶的光合色素含量的变化(平均值±标准偏差, n = 6)。 不同小写字母表示春小麦品种间差异显著(p = 0.05)。
Fig. 1 Variations in the content of photosynthetic pigments in the flag leaves of four wheat varieties during the heading stage (mean ± SD, n = 6). Different lower-case letters indicate significant differences among wheat varieties (p = 0.05).
图2 抽穗期4个高原春小麦品种旗叶的比叶干重(SLWd) (A)和比叶鲜重(SLWf) (B)的变化(平均值±标准偏差, n = 15)。 不同小写字母表示春小麦品种间的差异显著(p = 0.05)。
Fig. 2 Variations in the specific leaf dry weight (SLWd) (A) and specific leaf fresh weight (SLWf) (B) in the flag leaves of four wheat varieties during the heading stage (mean ± SD, n = 15). Different lower-case letters indicate significant differences among wheat varieties (p = 0.05).
图3 抽穗期全晴天3个典型时间段20分钟暗适应后4个高原春小麦品种旗叶的PSII最大光化学量子效率(Fv/Fm和1/Fo - 1/Fm)的变化(平均值±标准偏差, n = 12)。 不同小写字母表示4个春小麦品种在全晴天3个时间段之间的差异显著(p = 0.05)。
Fig. 3 Variations in the maximum photochemical efficiency of PSII (Fv/Fm and 1/Fo - 1/Fm) in the flag leaves of four wheat varieties after 20 min dark adaptation at three measurement times on a clear day during the heading stage (mean ± SD, n = 12). Different lower-case letters indicate significant differences among three typical times within wheat varieties on a clear day (p = 0.05).
图4 抽穗期全晴天3个典型时间段4个高原春小麦品种旗叶PSII反应中心叶绿素初始荧光产量(Fo)的变化(平均值±标准偏差, n = 12)。 不同小写字母表示4个春小麦品种在3个时间段之间的差异显著(p = 0.05)。
Fig. 4 Variations in the minimal fluorescence of PSII reaction centers (Fo) in the flag leaves of four wheat varieties after 20 min dark adaptation at three typical times during the heading stage (mean ± SD, n = 12). Different lower-case letters in each column indicate significant differences among three typical times within wheat varieties on a clear day (p = 0.05).
图5 抽穗期晴天稳定作用光下4个春小麦品种旗叶的PSII反应中心有效光化学量子效率(Fv′/Fm′)(A)和实际光化学量子效率(ΦPSII)(B)及上下午差异分析(平均值±标准偏差, n = 6)。 不同大写字母和小写字母分别表示上午和下午4个春小麦品种旗叶的Fv′/Fm′和ΦPSII的差异显著(p = 0.05)。ns, 上下午间无显著差异(p > 0.05); *和**, 上下午间差异显著和极显著(p < 0.05, p < 0.01)。
Fig. 5 Analysis of the PSII maximal photochemical efficiency (Fv′/Fm′) (A) and actual photochemical efficiency (ΦPSII) (B) in the flag leaves of four wheat varieties between morning and afternoon at a given light intensity during the heading stage (mean ± SD, n = 6). Different capital and lower-case letters in figures indicate significant differences in Fv′/Fm′ and ΦPSII, respectively, between morning and afternoon (p = 0.05). ns, no significant differences between morning and afternoon (p > 0.05); * and **, significant and highly significant differences between morning and afternoon (p < 0.05 and p < 0.01).
图6 抽穗期晴天稳定作用光下4个春小麦品种旗叶的PSII反应中心光化学猝灭系数(qP)(A)和非光化学猝灭系数(NPQ) (B)及上下午差异分析(平均值±标准偏差, n = 6)。 不同大写字母和小写字母分别表示上午和下午间4个春小麦品种旗叶的qP和NPQ的差异显著(p = 0.05)。ns, 上下午间无显著性差异(p > 0.05); *和**, 上下午间差异显著和极显著(p < 0.05和 p < 0.01)。
Fig. 6 Analysis of PSII photochemical quenching coefficient (qP) (A) and non-photochemical quenching coefficient (NPQ) (B) in the flag leaves of four wheat varieties between morning and afternoon at a given light intensity during the heading stage (means ± SD, n = 6). Different capital and lower-case letters in figures indicate significant differences in qP and NPQ, respectively, between morning and afternoon (p = 0.05). ns, no significant differences between morning and afternoon; * and **, significant and highly significant differences between morning and afternoon (p < 0.05 and p < 0.01).
图7 抽穗期高原春小麦品种旗叶PSII反应中心开放比率(qL)(A)及调节性能量耗散量子产量(ΦNPQ)(B)和非调节性能量耗散量子产量(ΦNO)(C)的上下午的变化(平均值±标准偏差, n = 6)。 不同大写字母和小写字母分别为上午和下午4个春小麦品种旗叶的qL、ΦNPQ和ΦNO差异显著(p = 0.05)。ns, 上下午间无显著性差异(p > 0.05); *和**, 上下午间差异显著和极显著(p < 0.05和p < 0.01)。
Fig. 7 Variations in the fraction of PSII reaction centers that are open (qL) (A), PSII regulatory energy dissipation in quantum yield (ΦNPQ) (B), and non-regulatory energy dissipation in quantum yield (ΦNO) (C) in the flag leaves of four wheat varieties between morning and afternoon at the given light intensity during the heading stage (means ± SD, n = 6). Different capital and lower-case letters in figures indicate significant differences in qL, ΦNPQ and ΦNO, respectively, between morning and afternoon (p = 0.05). ns, no significant differences between morning and afternoon; * and **, significant and highly significant differences between morning and afternoon (p < 0.05 and p < 0.01).
[1] |
Baker NR (2008). Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annual Review of Plant Biology, 59, 89-113.
DOI URL |
[2] |
Baker NR, Rosenqvist E (2004). Applications of chlorophyll fluorescence and improve crop production strategies: an examination of future possibilities. Journal of Experimental Botany, 55, 1607-1621.
DOI URL PMID |
[3] | Bilger W, Björkman O (1990). Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in leaves of Hedera canariensis. Photosynthesis Research, 25, 173-185. |
[4] | Butler WL (1978). Energy distribution in the photochemical apparatus of photosynthetic. Annual Review of Plant Physiology, 29, 345-378. |
[5] | Editor Committee of Historical Recorders of Dulan County (2001). Historical Recorders of Dulan County. Shaanxi People’s Press, Xi’an. 201-209. (in Chinese) |
[ 都兰县县志编委会 (2001). 都兰县志. 陕西人民出版社, 西安. 201-209.] | |
[6] |
Galvez-Valdivieso G, Fryer MJ, Lawson T, Slattery K, Truman W, Smimoff N, Asami T, Davies WJ, Jones AM, Baker NR, Mullineaux PM (2009). The high light response in Arabidopsis involves ABA signaling between vascular and bundle sheath cells. The Plant Cell, 21, 2143-2162.
URL PMID |
[7] | Genty B, Briantais JM, Baker NR (1989). The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. Biochimica et Biophysica Acta, 990, 87-92. |
[8] |
Govindjee (2002). A role for a light-harvesting antenna complex of photosystem II in photoprotection. The Plant Cell, 14, 1663-1668.
DOI URL PMID |
[9] |
Havaux M, Greppin H, Strasser RJ (1991). Functioning of photosystems I and II in pea leaves exposed to heat stress in the presence or absence of light. Planta, 186, 88-98.
URL PMID |
[10] | Hendrikson L, Furbank RT, Cow WS (2004). A simple alternative approach to assessing the fate of absorbed light energy using chlorophyll fluorescence. Photosynthesis Review, 82, 73-81. |
[11] |
Kramer DM, Johnson G, Kiirats O, Edwards GE (2004). New fluorescence parameters for the determination of QA redox state and excitation energy fluxes. Photosynthesis Research, 79, 209-218.
URL PMID |
[12] | Larcher W (1980). Physiological Plant Ecology. 2nd edn. Springer-Verlag, New York. 5-60. |
[13] |
Lima Neto MC, Lobo AKM, Martins MO, Fontenele AV, Silveira JAG (2014). Dissipation of excess photosynthetic energy contributes to salinity tolerance: a comparative study of salt-tolerant Ricinus communis and salt-sensitive Jatropha curcas. Journal of Plant Physiology, 171, 23-30.
DOI URL PMID |
[14] |
Maxwell K, Johnson GN (2000). Chlorophyll fluorescence―a practical guide. Journal of Experimental Botany, 51, 659-668.
DOI URL PMID |
[15] | Middleton EM, Teramura AH (1993). The role of flavonol glycoside and carotenoids in protecting soybean from ultraviolet-B damage. Plant Physiology, 103, 475-480. |
[16] |
Murchie EH, Niyogi KK (2011). Manipulation of photoprotec- tion to improve plant photosynthesis. Plant Physiology, 155, 86-92.
DOI URL PMID |
[17] |
Niyogi KK, Truong TB (2013). Evolution of flexible non- photochemical quenching mechanisms that regulate light harvesting in oxygenic photosynthesis. Current Opinion in Plant Biology, 16, 307-314.
DOI URL PMID |
[18] | Oxborough K, Baker NR (1997). Resolving chlorophyll a fluor- escence images of photosynthetic efficiency into photo- chemical and non-photochemical components: calculation of qP and Fv′/Fm′ without measuring Fo′. Photosynthesis Research, 54, 135-142. |
[19] | Quick WP, Stitt M (1989). An examination of factors con- tributing to non-photochemical quenching of chlorophyll fluorescence in barley leaves. Biochimica et Biophysica Acta, 977, 287-296. |
[20] |
Sáez PL, Bravo LA, Latsague MI, Toneatti MJ, Sánchez-Olate M, Ríos DG (2013). Light energy management in micropropagated plants of Castanea sativa, effects of photoinhibition. Plant Science, 201, 12-24.
DOI URL PMID |
[21] |
Shi SB, Shang YX, Zhu PJ, Yang L, Zhang B (2011). Effects of solar UV-B radiation on the efficiency of PSII photochemistry in the alpine plant Saussurea superba under different weather conditions in the Qinghai-Tibet Plateau of China. Chinese Journal of Plant Ecology, 35, 741-750. (in Chinese with English abstract)
DOI URL |
[ 师生波, 尚艳霞, 朱鹏锦, 杨莉, 张波 (2011). 不同天气类型下UV-B辐射对高山植物美丽风毛菊叶片PSII光化学效率的影响分析. 植物生态学报, 35, 741-750.] | |
[22] | Shi SB, Zhu WY, Li HM, Zhou DW, Han F, Zhao XQ, Tang YH (2004). Photosynthesis of Saussurea superba and Gentiana straminea is not reduced after long-term enhancement of UV-B radiation. Environmental and Experimental Botany, 51, 75-83. |
[23] | Su TZ, Pan JS (1981). An analysis of the physiological feature of the higher yielding ability of spring wheat in the Xiangride farm, Qinghai Province. Acta Agronomica Sinica, 7, 19-25. (in Chinese with English abstract) |
[ 苏悌之, 潘锦珊 (1981). 青海香日德春小麦高产的生理特性分析. 作物学报, 7, 19-25.] | |
[24] | Sun HL (2007). Progress of ecological techniques being the source of sustainable high yield of crop—the inspiration from the high yield fields of wheat in Xiangride area of Qinghai Province after revisiting. Chinese Journal of Eco-Agriculture, 15, 181-183. (in Chinese with English abstract) |
[ 孙鸿良 (2007). 生态技术进步造就了作物高产不衰的典型—重访青海香日德地区春小麦高产田的启示. 中国生态农业学报, 15, 181-183.] | |
[25] |
Takahashi S, Milward SE, Yamori W, Evans JR, Hillier W, Badger MR (2010). The solar action spectrum of photosystem II damage. Plant Physiology, 153, 988-993.
DOI URL PMID |
[26] |
Tikkanen M, Mekala NR, Aro EM (2013). Photosystem II photoinhibition-repair cycle protects photosystem I from irreversible damage. Biochimica et Biophysica Acta, 1837, 210-215.
URL PMID |
[27] | Xu DQ (2002). Photosynthetic Efficiency. Shanghai Scientific and Technical Press, Shanghai. (in Chinese) |
[ 许大全 (2002). 光合作用效率. 上海科学技术出版社, 上海.] | |
[28] |
Yamori W, Hikosaka K, Way DA (2014). Temperature response of photosynthesis in C3, C4, and CAM plants: temperature acclimation and temperature adaptation. Photosynthesis Research, 119, 101-117.
URL PMID |
[29] | Yu BH, Lü CH (2011). Assessment of ecological vulnerability on the Tibetan Plateau. Geographical Research, 30, 2289-2294. (in Chinese with English abstract) |
[ 于伯华, 吕昌河 (2011). 青藏高原高寒区生态脆弱性评价. 地理研究, 30, 2289-2294.] | |
[30] | Zhang SR (1999). A discussion on chlorophyll fluorescence kinetics parameters and their significance. Chinese Bulletin of Botany, 16, 444-448. (in Chinese with English abstract) |
[ 张守仁 (1999). 叶绿素荧光动力学参数的意义及讨论. 植物学通报, 16, 444-448.] | |
[31] | Zhu GL, Zhong HW, Zhang AQ (1990). Plant Physiological Experiment. Beijing University Press, Beijing. 51-54. (in Chinese) |
[ 朱广廉, 钟诲文, 张爱琴 (1990). 植物生理学实验. 北京大学出版社, 北京. 51-54.] |
[1] | 赵艳超, 陈立同. 土壤养分对青藏高原高寒草地生物量响应增温的调节作用[J]. 植物生态学报, 2023, 47(8): 1071-1081. |
[2] | 任培鑫, 李鹏, 彭长辉, 周晓路, 杨铭霞. 洞庭湖流域植被光合物候的时空变化及其对气候变化的响应[J]. 植物生态学报, 2023, 47(3): 319-330. |
[3] | 师生波, 周党卫, 李天才, 德科加, 杲秀珍, 马家麟, 孙涛, 王方琳. 青藏高原高山嵩草光合功能对模拟夜间低温的响应[J]. 植物生态学报, 2023, 47(3): 361-373. |
[4] | 师生波, 师瑞, 周党卫, 张雯. 低温对高山嵩草叶片光化学和非光化学能量耗散特征的影响[J]. 植物生态学报, 2023, 47(10): 1441-1452. |
[5] | 林马震, 黄勇, 李洋, 孙建. 高寒草地植物生存策略地理分布特征及其影响因素[J]. 植物生态学报, 2023, 47(1): 41-50. |
[6] | 朱玉英, 张华敏, 丁明军, 余紫萍. 青藏高原植被绿度变化及其对干湿变化的响应[J]. 植物生态学报, 2023, 47(1): 51-64. |
[7] | 魏瑶, 马志远, 周佳颖, 张振华. 模拟增温改变青藏高原植物繁殖物候及植株高度[J]. 植物生态学报, 2022, 46(9): 995-1004. |
[8] | 金伊丽, 王皓言, 魏临风, 侯颖, 胡景, 吴铠, 夏昊钧, 夏洁, 周伯睿, 李凯, 倪健. 青藏高原植物群落样方数据集[J]. 植物生态学报, 2022, 46(7): 846-854. |
[9] | 卢晶, 马宗祺, 高鹏斐, 樊宝丽, 孙坤. 祁连山区演替先锋物种西藏沙棘的种群结构及动态对海拔梯度的响应[J]. 植物生态学报, 2022, 46(5): 569-579. |
[10] | 胡潇飞, 魏临风, 程琦, 吴星麒, 倪健. 青藏高原地区气候图解数据集[J]. 植物生态学报, 2022, 46(4): 484-492. |
[11] | 吴赞, 彭云峰, 杨贵彪, 李秦鲁, 刘洋, 马黎华, 杨元合, 蒋先军. 青藏高原高寒草地退化对土壤及微生物化学计量特征的影响[J]. 植物生态学报, 2022, 46(4): 461-472. |
[12] | 郑周涛, 张扬建. 1982-2018年青藏高原水分利用效率变化及归因分析[J]. 植物生态学报, 2022, 46(12): 1486-1496. |
[13] | 吴霖升, 张永光, 章钊颖, 张小康, 吴云飞. 日光诱导叶绿素荧光遥感及其在陆地生态系统监测中的应用[J]. 植物生态学报, 2022, 46(10): 1167-1199. |
[14] | 薛金儒, 吕肖良. 黄土高原生态工程实施下基于日光诱导叶绿素荧光的植被恢复生产力效益评价[J]. 植物生态学报, 2022, 46(10): 1289-1304. |
[15] | 刘宁, 彭守璋, 陈云明. 气候因子对青藏高原植被生长的时间效应[J]. 植物生态学报, 2022, 46(1): 18-26. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||
Copyright © 2022 版权所有 《植物生态学报》编辑部
地址: 北京香山南辛村20号, 邮编: 100093
Tel.: 010-62836134, 62836138; Fax: 010-82599431; E-mail: apes@ibcas.ac.cn, cjpe@ibcas.ac.cn
备案号: 京ICP备16067583号-19