利用叶绿素荧光和820 nm光反射同步测量揭示枇杷叶片PSII和PSI冬季光抑制差异

doi:10.17521/cjpe.2024.0309

摘要/Abstract

摘要：

枇杷(Eriobotrya japonica)属于常绿乔木植物, 在冬季由于低温胁迫易发生光抑制或光破坏, 进而影响其生长。该研究利用叶绿素荧光和820 nm光反射同步测量技术, 比较了冬季枇杷叶片两个光系统光抑制的差异。由秋入冬后, 枇杷叶片光系统II (PSII)最大量子效率(F_v/F_m)显著下降, 但PSII供体侧和受体侧的光合电子传递能力不变, 光系统I (PSI)活性没有变化; PSII和PSI的实际光化学效率(Φ_PSII和Φ_PSI)均显著下降, 非光化学猝灭(NPQ)在强光下显著下降。由冬入春后, 枇杷叶片F_v/F_m显著升高, PSII受体侧的电子传递能力和PSI活性下降。并且, Φ_PSII显著上升, 而Φ_PSI保持不变, NPQ在强光下显著升高。这表明, 冬季低温胁迫下, 枇杷叶片PSI的光抑制发生比PSII晚, 且恢复较慢。PSII光抑制发生较早, 其活性恢复也较早。

关键词: 枇杷, 叶绿素荧光, 820 nm光反射, 光抑制

Abstract:

Aims Loquat (Eriobotrya japonica) is evergreen tree that often suffers photoinhibition or photodamage at low temperature in winter, which affects its growth. Therefore, this study explored the photoinhibition of two photosystems of loquat leaves in winter.
Methods In this study, the photoinhibition of two photosystems of loquat leaves in winter was compared using simultaneous measurements of chlorophyll fluorescence and 820 nm light reflection.
Important findings From autumn to winter, the maximum quantum yield of photosystem II (PSII) (F_v/F_m) in loquat leaves decreased significantly, while the photosynthetic electron transfer capacity at both the donor and acceptor sides of PSII did not change. The activity of photosystem I (PSI) did not change either. However, the actual photochemical efficiencies of both PSII and PSI decreased significantly. Non-photochemical quenching decreased significantly under high light. From winter to spring, the value of F_v/F_m in loquat leaves increased significantly. The electron transfer capacity at the acceptor side of PSII and the activity of PSI also decreased. Moreover, the actual photochemical efficiency of PSII increased significantly, while that of PSI remained unchanged. Non-photochemical quenching increased significantly under high light. These results indicate that the photoinhibition of PSI occurred later than PSII in loquat leaves in winter. The activity of PSI also recovered slowly. Photoinhibition of PSII occurred earlier, and its activity recovered earlier.

Key words: Eriobotrya japonica, chlorophyll fluorescence, 820 nm light reflection, photoinhibition

张雯婷, 张国云, 裴国亮, 李鹏民. 利用叶绿素荧光和820 nm光反射同步测量揭示枇杷叶片PSII和PSI冬季光抑制差异. 植物生态学报, 2025, 49(7): 1156-1162. DOI: 10.17521/cjpe.2024.0309

ZHANG Wen-Ting, ZHANG Guo-Yun, PEI Guo-Liang, LI Peng-Min. Different photoinhibition of PSII and PSI in Eriobotrya japonica leaves in winter revealed by simultaneous measurements of chlorophyll fluorescence and 820 nm light reflection. Chinese Journal of Plant Ecology, 2025, 49(7): 1156-1162. DOI: 10.17521/cjpe.2024.0309

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2024.0309

https://www.plant-ecology.com/CN/Y2025/V49/I7/1156

图/表 3

图1 不同季节枇杷叶片叶绿素荧光诱导动力学(A)和820 nm光反射动力学曲线(B)。图中每条曲线为5次测定的平均值。MR0, 820 nm光反射起始点; MRmin, 820 nm光反射最小值; MRmax, 820 nm光反射最大值。

Fig. 1 Induction kinetics of chlorophyll a fluorescence (A) and 820 nm reflection (B) in Eriobotrya japonica leaves growing in different seasons. Each curve was the average of five replicates. MR0, initial point of 820 nm reflection; MRmin, minimum of 820 nm reflection; MRmax, maximum of 820 nm reflection.

图2 不同季节枇杷叶片叶绿素荧光诱导动力学(A)和820 nm光反射动力学(B)参数(平均值±标准误, n = 5)。不同小写字母表示同一参数在不同季节具有显著差异(p < 0.05, LSD)。Fv/Fm, PSII最大量子效率; Wk, K点可变荧光; Vi, I点可变荧光; Vj, J点可变荧光; VPSI, 820 nm光反射最大下降斜率; VPSII-PSI, 820 nm光反射最大上升斜率。

Fig. 2 Parameters derived from chlorophyll a fluorescence (A) and 820 nm reflection (B) kinetics in Eriobotrya japonica leaves growing in different seasons (mean ± SE, n = 5). Different lowercase letters above the bars indicate significant difference for same parameter among different seasons (p < 0.05, LSD). Fv/Fm, maximum quantum yield of PSII; Wk, relative variable fluorescence at K-step; Vi, relative variable fluorescence at I-step; Vj, relative variable fluorescence at J-step; VPSI, maximum decrease slope of 820 nm reflection; VPSII-PSI, maximum increase slope of 820 nm reflection.

图3 不同季节枇杷叶片光下两个光系统的实际光化学效率及热耗散(平均值±标准误, n = 5)。ΦPSII, 光系统II实际光化学效率; ΦPSI, 光系统I实际光化学效率; NPQ, 非光化学猝灭; PPFD, 光合光通量密度。

Fig. 3 Actual photochemical efficiencies of two photosystems and non-photochemical quenching in Eriobotrya japonica leaves under light conditions in different seasons (mean ± SE, n = 5). ΦPSII, actual photochemical efficiency of PSII; ΦPSI, actual photochemical efficiency of PSI; NPQ, non-photochemical quenching; PPFD, photosynthetic photon flux density.

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