不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系

doi:10.3773/j.issn.1005-264x.2009.03.017

植物生态学报 ›› 2009, Vol. 33 ›› Issue (3): 580-586.DOI: 10.3773/j.issn.1005-264x.2009.03.017

不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系

韦泽秀¹^,⁴, 梁银丽¹^,²^,^*(), 山田智³, 曾兴权⁴, 周茂娟¹, 黄茂林¹, 吴燕¹

1 西北农林科技大学资源环境学院,陕西杨凌 712100
2 中国科学院水利部水土保持研究所,陕西杨凌 712100
3 日本鸟取大学农学部,日本鸟取 680-8553
4 西藏自治区农牧科学院农业研究所,拉萨 850002

收稿日期:2008-10-09 接受日期:2009-01-06 出版日期:2009-10-09 发布日期:2009-05-31
通讯作者: 梁银丽
作者简介:*E-mail: liangyl@ms.iswc.ac.cn
基金资助:
中国科学院西部行动项目(KZCX2-XB2-05-01);中国科学院知识创新重要方向项目(KZCX2-YW-443-3);国家科技支撑项目(2006BAD09B07);中国科学院安塞站和中国科学院水土保持研究所领域前沿项目(SW04302)

RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS

WEI Ze-Xiu¹^,⁴, LIANG Yin-Li¹^,²^,^*(), YAMADA Satoshi³, ZENG Xing-Quan⁴, ZHOU Mao-Juan¹, HUANG Mao-Lin¹, WU Yan¹

¹College of Resource and Environment, Northwest Sci &Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China
²Institute of Water and Soil Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling, Shaanxi 712100, China
³Faculty of Agriculture, Tottori University 4-101 Koyamach-Minama, 680-8553, Japan
⁴Tibet Agriculture Research Institute, Lhasa 850032, China

Received:2008-10-09 Accepted:2009-01-06 Online:2009-10-09 Published:2009-05-31
Contact: LIANG Yin-Li

摘要/Abstract

摘要：

采用内含子切接点引物 (Intron-splice junction primer) 和长随机引物的PCR (Polymerase chain reaction, 聚合酶链) 标记技术, 就黄土高原不同水肥处理对日光温室番茄土壤微生物群落多样性进行了研究, 并对产量、品质的影响进行了分析。结果表明, 所用的6个引物共能扩增出182条稳定清晰的条带, 其中142条为多态性条带, 多态性条带为78.02%。聚类分析与主坐标分析表明, 水肥对土壤微生物群落多样性的变化有不同的影响。土壤微生物群落多样性指数与番茄产量、果实的Vc、可溶性蛋白质含量成正相关, 尤其与Vc的相关系数达0.9211, 而与可溶性固形物含量成负相关关系。6个水肥组合中WmFh (中水高肥) 土壤微生物群落多样性指数最高, 且番茄果实的Vc、可溶性蛋白质含量及产量显著高于其他处理, 可溶性固形物含量较低。该组合有利于土壤微生物群落的多样性和稳定性的提高, 利于土壤生态环境的改善和番茄优良品质的形成。

关键词: 内含子切接点引物, 多样性指数, 产量, 品质

Abstract:

Aims Our objective was to investigate diversity within soil microbial communities and tomato yields and qualities under different soil water and fertilization treatments.

Methods PCR (polymerase chain reaction) with long random primers and intron-splice junction primers were used to study greenhouse soil microbial diversity with tomato cultivated under different treat-ments.

Important findings A total of 182 bands were detected with 6 primer combinations, and 142 (78.02%) of the bands were polymorphic. Soil microbial community diversity was affected by the different soil water contents and fertilizations. Diversity was positively correlated with yields, VC contents and solu-ble protein contents, but negatively correlated with soluble solids contents. The highest soil microbial community diversity index was detected under the treatment of medium water level and high fertilization; the VC contents, soluble protein contents and yields of tomato fruits were significantly higher and the soluble solids of the tomato fruits was lower than those in other treatments. This showed that treatment can enhance the soil microbial community diversity and stability and to improve soil ecological environments and tomato quality.

Key words: intron-splice junction primer, Shannon-Wiener index, yield, quality

韦泽秀, 梁银丽, 山田智, 曾兴权, 周茂娟, 黄茂林, 吴燕. 不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系. 植物生态学报, 2009, 33(3): 580-586. DOI: 10.3773/j.issn.1005-264x.2009.03.017

WEI Ze-Xiu, LIANG Yin-Li, YAMADA Satoshi, ZENG Xing-Quan, ZHOU Mao-Juan, HUANG Mao-Lin, WU Yan. RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS. Chinese Journal of Plant Ecology, 2009, 33(3): 580-586. DOI: 10.3773/j.issn.1005-264x.2009.03.017

导出引用管理器 EndNote|Ris|BibTeX

链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.03.017

https://www.plant-ecology.com/CN/Y2009/V33/I3/580

图/表 9

表1 日光温室内0~20 cm土壤化学特性

Table 1 The chemical properties of soil layer 0~20 cm in the greenhouse

图1 安塞站内日光温室4至10月平均温度和相对湿度变化

Fig.1 Mean temperature and RH from April to October in greenhouhouse at Ansai station

表2 引物序列及扩增结果

Table 2 The sequence of the primers and the result of amplified

图2 不同水肥处理PCR扩增结果聚类图 Wh Fh、Wm Fh、Wl Fh处理:土壤相对含水量分别为90%~100%, 70%~80%, 50%~60%, 施肥量600 kg·hm-2N和420 kg·hm-2P2O5 In Wh Fh, Wm Fh, Wl Fh treatments, soil relative water content were 90%-100%, 70%-80%, 50%-60%respectively and fertilizer amount were 600kg·hm-2N and 420 kg·hm-2P2O5;Wh Fl、Wm Fl、Wl Fl处理:土壤相对含水量分别为90%~100%, 70%~80%, 50%~60%, 施肥量420 kg·hm-2N和294 kg·hm-2P2O5;In Wh Fl, Wm Fl, Wl Fl treatments, soil relative water content were 90%-100%, 70%-80%, 50%-60%respectively and fertilizer amount were 420 kg·hm-2N and 294 kg·hm-2P2O5

Fig.2 The dendrogram of different water and fertiliza-tion treatment based on PCR pattern

图3 6个不同水肥处理的主坐标分析图注同图2

Fig.3 2D plots of principal coordinate analysis of six different treatment based on PCR data using NTSYS-pc program Note see Fig.2

图4 不同处理土壤微生物遗传多样性指数图注同图2

Fig.4 The soil microbial Shannon-wiener index of different treatments Note see Fig.2

图5 L1内含子切接点引物扩增图图注同图2

Fig.5 The amplification map of L1 intron-splice junction primer Note see Fig.2

表3 不同水肥处理的番茄产量与品质

Table 3 The yield and fruit nutrient quality in different treatments

表4 土壤微生物群落多样性指数与番茄产量、品质相关分析结果

Table 4 The correlation between soil microbial community diversity index with tomato yield and fruit quality

参考文献 22

[1]	Albiach R, Canet R, Pomares F, Ingelmo F (2000). Micro-bial biomass content and enzymatic activities after the application of organic amendments to a horticultural soil. Bioresource Technology, 75,43-48.
[2]	Bums RG (1982). Enzyme activity in soil:location and a possible role in microbial ecology. Soil Biology&Biochemistry, 14,423-427.
[3]	de Barros LM, Soden A, Henschke PA, Langridhe P (1996). PCR differentiation of commercial yeast strains using intron splice site primers. Applied and Environmental Microbiology, 62,4514-4520.
[4]	Huang PM, Bollag JM, Senesi N (2002). Interactions Be-tween Soil Particles and Microorganisms-Impact on the Terrestrial Ecosystem. Marcel Dekker, New York,307-379.
[5]	Kennydy AC, Smith KL (1995). Soil microbial diversity index and the sustainability of agricultural soils. Plant and Soil, 170,75-86.
[6]	Ma WL (马万里), Tibbits J, Adams M (2004). A new method for reserch on soil microbial diversity. Acta Pedologica Sinica (土壤学报), 41,103-107. (in Chinese with English abstract)
[7]	Marcote I, Hernandez T, Garcia C, Polo A (2001). Alfredo polo influence of one or two successive annual appli-cations of organic fertilisers on the enzyme activity of a soil under barley cultivation. Bioresource Technol-ogy, 79,147-154.
[8]	Meng FL (孟凡磊), Qiang XL (强小林), She KJ (佘奎军), Tang YW (唐亚伟), Hu YG (胡银岗) (2007). Genetic diversity analysis among hulless barley varieties from the major agricultural areas of Tibet. Acta Agronomica Sinica (作物学报), 33,1910-1914. (in Chinese with English abstract)
[9]	Nei M, Li WH (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America. 76,5269-5273.
[10]	Qu ZH (曲再红), Du XG (杜相革) (2004). Study on the interaction among soil additive, soil microorganism and tomato seedling stage. Chinese Agricultural Sci-ence Bulletin (中国农学通报) 20 (4),84-86. (in Chinese with English abstract)
[11]	Rafalski A, Gidzińska M, Wińniewska I (1997). PCR-based systems for evaluation of relationships among maize inbreds.In:Tsaftaris A ed.Genetics, Biotechnology and Breeding of Maize and Sorghum. The Royal Soci-ety of Chemistry, Cambridge, 106-111.
[12]	Schutter M, Sandeno J, Dick R (2001). Seasonal, soil type, alternative management influences on microbial com-munities of vegetable cropping systems. Biology and Fertility of Soils, 34,397-410.
[13]	Shannon CE, Weaver W (1949). A Mathematical Theory of Communication. The University of Illinois Press, Urbana,3-14.
[14]	Weining S, Henry RJ (1995). Molecular analysis of the DNA polymorphism of wild barley (Hordeum spon-taneum) germplasm using the polymerase chain reac-tion. Genetic Resources and Crop Evolution, 42,273-281.
[15]	Weining S, Ko L, Henry RJ (1994). Polymorphisms in theα-amy1gene of wild and cultivated barley revealed by the polymerase chain reaction. Theoretical and Applied Genetics, 89,509-513.
[16]	Weining S, Langridge P (1991). Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theoretical and Applied Genetics, 82,209-216.
[17]	Wu FZ (吴凤芝), Wang XZ (王学征) (2007). Effect of monocropping and rotation on soil microbial commu-nity diversity and cucumber yield and quality under protected cultivation. Scientia Agricultura Sinica (中国农业科学), 40,2274-2280. (in Chinese with English abstract)
[18]	Xu HQ (徐华勤), Xiao RL (肖润林), Zou DS (邹冬生), Song TQ (宋同清), Luo W (罗文), Li SH (李盛华) (2007). Effects of long-term fertilization on functional diversity of soil microbial community of the tea plan-tation. Acta Ecologica Sinica (生态学报), 27,3355-3361. (in Chinese with English abstract)
[19]	Yao H, He Z, Wilson MJ, Campbell CD (2000). Microbial biomass and community structure in a sequence of soils with increasing fertility and changing land use. Microbial Ecology, 40,223-237.
[20]	Yao J (姚健), Yang YH (杨永华), Shen XR (沈晓蓉), Lu WZ (陆维忠) (2000). A preliminary study on DNA sequence diversity of soil microbial community af-fected by agricultural chemicals. Acta Ecologica Sinica (生态学报), 20,1020-1027. (in Chinese withEnglish abstract).
[21]	Yin R (尹睿), Zhang HY (张华勇), Huang JF (黄锦法), Lin XG (林先贵), Wang JH (王俊华), Cao HZ (曹洪志) (2004). Comparison of microbiological properties be-tween soils of rice-wheat rotation and vegetable culti-vation. Plant Nutrition and Fertilizer Science (植物营养与肥料), 10 (1),57-62. (in Chinese with English ab-stract).
[22]	Zhang FH (张风华), Ma FY (马富裕), Zheng Z (郑重), Jin T (金涛), Ma S (马旭) (2000). A study of cotton growth and rhizospheric microorganism on the differ-ent water and fertilizer under mulch drip irrigation. Journal of Xinjiang Agricultural University (新疆农业大学学报), 23 (4),56-58. (in Chinese with English abstract)

不同水肥条件下番茄土壤微生物群落多样性及其与产量品质的关系

RELATION OF SOIL MICROBIAL DIVERSITY TO TOMATO YIELD AND QUALITY UNDER DIFFERENT SOIL WATER CONDITIONS AND FERTILIZATIONS

RichHTML

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

图/表 9

参考文献 22

相关文章 15

编辑推荐

Metrics

本文评价

[1]	熊淑萍, 曹文博, 曹锐, 张志勇, 付新露, 徐赛俊, 潘虎强, 王小纯, 马新明. 水平结构配置对冬小麦冠层垂直结构、微环境及产量的影响[J]. 植物生态学报, 2022, 46(2): 188-196.
[2]	赵晏平, 王忠武, 温都日根, 赵玉金, 白永飞. 基于Sentinel-2数据的草地植物功能多样性遥感反演及其与生产力的关系[J]. 植物生态学报, 2022, 46(10): 1234-1250.
[3]	孙浩哲, 王襄平, 张树斌, 吴鹏, 杨蕾. 阔叶红松林不同演替阶段凋落物产量及其稳定性的影响因素[J]. 植物生态学报, 2021, 45(6): 594-605.
[4]	叶学华, 薛建国, 谢秀芳, 黄振英. 外部干扰对根茎型克隆植物甘草自然种群植株生长及主要药用成分含量的影响[J]. 植物生态学报, 2020, 44(9): 951-961.
[5]	刘雪飞, 吴林, 王涵, 洪柳, 熊莉军. 鄂西南亚高山湿地泥炭藓的生长与分解[J]. 植物生态学报, 2020, 44(3): 228-235.
[6]	唐丽丽, 杨彤, 刘鸿雁, 康慕谊, 王仁卿, 张峰, 高贤明, 岳明, 张梅, 郑璞帆, 石福臣. 华北地区荆条灌丛分布及物种多样性空间分异规律[J]. 植物生态学报, 2019, 43(9): 825-833.
[7]	刘媛媛, 马进泽, 卜兆君, 王升忠, 张雪冰, 张婷玉, 刘莎莎, 付彪, 康媛. 地理来源与生物化学属性对泥炭地植物残体分解的影响[J]. 植物生态学报, 2018, 42(7): 713-722.
[8]	刘璐, 赵常明, 徐文婷, 申国珍, 谢宗强. 神农架常绿落叶阔叶混交林凋落物动态及影响因素[J]. 植物生态学报, 2018, 42(6): 619-628.
[9]	张鑫, 邢亚娟, 闫国永, 王庆贵. 细根对降水变化响应的meta分析[J]. 植物生态学报, 2018, 42(2): 164-172.
[10]	郑成岩, 邓艾兴, LATIFMANESHHojatollah, 宋振伟, 张俊, 王利, 张卫建. 增温对青藏高原冬小麦干物质积累转运及氮吸收利用的影响[J]. 植物生态学报, 2017, 41(10): 1060-1068.
[11]	王丹, 乔匀周, 董宝娣, 葛静, 杨萍果, 刘孟雨. 昼夜不对称性与对称性升温对大豆产量和水分利用的影响[J]. 植物生态学报, 2016, 40(8): 827-833.
[12]	邹长明, 王允青, 刘英, 张晓红, 唐杉. 四种豆科作物的光合生理和生长发育对弱光的响应[J]. 植物生态学报, 2015, 39(9): 909-916.
[13]	孟德云, 侯林琳, 杨莎, 孟静静, 郭峰, 李新国, 万书波. 外源多胺对盆栽花生盐胁迫的缓解作用[J]. 植物生态学报, 2015, 39(12): 1209-1215.
[14]	陈云玉, 熊德成, 黄锦学, 王韦韦, 胡双成, 邓飞, 许辰森, 冯建新, 史顺增, 钟波元, 陈光水. 中亚热带不同演替阶段的马尾松和米槠人工林的细根生产量研究[J]. 植物生态学报, 2015, 39(11): 1071-1081.
[15]	张佳蕾, 郭峰, 孟静静, 于晓霞, 杨莎, 张思斌, 耿耘, 李新国, 万书波. 酸性土施用钙肥对花生产量和品质及相关代谢酶活性的影响[J]. 植物生态学报, 2015, 39(11): 1101-1109.