植物生态学报 ›› 2011, Vol. 35 ›› Issue (3): 330-336.DOI: 10.3724/SP.J.1258.2011.00330

• 研究论文 • 上一篇    下一篇

西南地区麻疯树天然种群遗传多样性的等位酶变异

张炜1,2, 罗建勋2, 辜云杰2, 胡庭兴1,*()   

  1. 1四川农业大学林学院, 四川省林业生态工程省级重点试验室, 四川雅安 625014
    2四川省林业科学研究院, 成都 610081
  • 收稿日期:2010-06-04 接受日期:2010-11-22 出版日期:2011-06-04 发布日期:2011-03-02
  • 通讯作者: 胡庭兴
  • 作者简介:*E-mail: hutx001@yahoo.com.cn

Allozyme variation of genetic diversity in natural populations of Jatropha curcas germplasm from different areas in southwest China

ZHANG Wei1,2, LUO Jian-Xun2, GU Yun-Jie2, HU Ting-Xing1,*()   

  1. 1Key Laboratory of Forestry Ecological Engineering of Sichuan Province, College of Forestry, Sichuan Agricultural University, Ya’an, Sichuan 625014, China
    2Sichuan Academy of Forestry, Chengdu 610081, China
  • Received:2010-06-04 Accepted:2010-11-22 Online:2011-06-04 Published:2011-03-02
  • Contact: HU Ting-Xing

摘要:

为了揭示麻疯树(Jatropha curcas)天然种群的遗传多样性和遗传结构, 采用聚苯烯酰胺凝胶垂直平板电泳技术, 对采自四川、云南、贵州3个省的10个麻疯树天然种群的叶片样本进行了同工酶分析。7个酶系统10个位点的检测结果表明: 麻疯树种群水平上的遗传多样性较高, 每位点平均等位基因数为2.428 6, 多态位点百分率为97.14%, 平均期望杂合度为0.396 4。种群间遗传分化系数为0.041 3, 种群间总的基因流较高, 为5.808 9, 种群间遗传一致度较高(Shannon信息指数为0.921 7- 0.995 3)。非加权类平均法(UPGMA)聚类结果显示, 10个种群的遗传距离与地理距离相关性不显著。麻疯树天然种群具有较低程度的遗传分化、较高的基因流, 种内及种群内多样性丰富, 这为麻疯树优良品种的选育提供了良好的遗传基础。

关键词: 同工酶, 遗传多样性, 麻疯树

Abstract:

Aims Our objectives was to investigate the genetic structure and diversity of ten natural populations of Jatropha curcas.
Methods Seven loci encodings were detected by vertical polyacrylamide gel electrophoresis.
Important findings The genetic diversity of J. curcas was high at the species level with a mean number of alleles per locus of 2.428 6, percentage of polymorphic loci of 97.14% and a mean expected heterozygosity of 0.396 4. A low level of genetic differentiation among populations (0.041 3) and a high estimate of gene flow (5.808 9) were detected together with a high level of genetic identity among populations (0.921 7-0.995 3). Unweighted pair-group mean arithmetic cluster analysis suggested that the genetic distances among populations were weakly correlated with their geographic distances. The results provided a good genetic base to select varieties.

Key words: allozyme, genetic diversity, Jatropha curcas