不同氮水平下内生固氮菌对入侵植物南美蟛蜞菊生长策略的影响

doi:10.17521/cjpe.2022.0004

植物生态学报 ›› 2023, Vol. 47 ›› Issue (2): 195-205.DOI: 10.17521/cjpe.2022.0004 cstr: 32100.14.cjpe.2022.0004

所属专题：入侵生态学

不同氮水平下内生固氮菌对入侵植物南美蟛蜞菊生长策略的影响

汪晶晶¹, 王嘉浩², 黄致云¹, Vanessa Chiamaka OKECHUKW¹, 胡蝶², 祁珊珊²^,^*(), 戴志聪¹^,³, 杜道林¹

¹江苏大学环境与安全工程学院, 江苏镇江 212013
²江苏大学农业工程学院现代农业装备与技术教育部重点实验室, 江苏镇江 212013
³江苏省水处理技术与材料协同创新中心, 苏州科技学院, 江苏苏州 215009

收稿日期:2022-01-05 接受日期:2022-05-20 出版日期:2023-02-20 发布日期:2023-02-28
基金资助:
江苏省自然科学基金(BK20211321);国家自然科学基金(32171509);国家自然科学基金(32071521);江苏省博士后科学基金(2021K160B);江苏省高校优势学科项目

Effects of endophytic nitrogen-fixing bacteria on the growth strategy of an invasive plant Sphagneticola trilobata under different nitrogen levels

WANG Jing-Jing¹, WANG Jia-Hao², HUANG Zhi-Yun¹, Vanessa Chiamaka OKECHUKW¹, HU Die², QI Shan-Shan²^,^*(), DAI Zhi-Cong¹^,³, DU Dao-Lin¹

¹School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China
²Key Laboratory of Modern Agricultural Equipment and Technology, Ministry of Education, School of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China
³Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment, Suzhou University of Science and Technology, Suzhou, Jiangsu 215009, China

Received:2022-01-05 Accepted:2022-05-20 Online:2023-02-20 Published:2023-02-28
Supported by:
Natural Science Foundation of Jiangsu Province(BK20211321);National Natural Science Foundation of China(32171509);National Natural Science Foundation of China(32071521);Postdoctoral Science Foundation of Jiangsu Province(2021K160B);Priority Academic Program Development of Jiangsu Higher Education Institutions

摘要/Abstract

摘要：

很多因素可以影响外来植物的入侵性, 其中内生菌可能扮演了极其重要的角色。为了探究内生固氮菌对入侵植物生长策略的影响, 本研究分别在高和低两种氮水平下, 种植接种和不接种内生固氮菌的入侵植物南美蟛蜞菊(Sphagneticola trilobata)和同属本地植物蟛蜞菊(S. calendulacea), 对比研究它们的生长和氮含量。从南美蟛蜞菊茎段分离出内生固氮菌Kosakonia sp. WTB-JS007, 发现其对南美蟛蜞菊和蟛蜞菊这两种植物生长策略的影响存在显著差异, 且这种差异不受氮水平的影响。在低氮处理下, 接种WTB-JS007对蟛蜞菊的生长及植株氮含量没有显著影响, 但显著提高了南美蟛蜞菊的地上生物量(30.48%), 促进了匍匐茎的伸长, 减少了地下生物量(56.58%), 并提高了植株总氮含量(47.51%)。类似地, 在高氮水平下, 内生固氮菌亦显著影响南美蟛蜞菊地上部分的生长, 但对蟛蜞菊生长的影响不显著。这些结果表明, 内生固氮菌对入侵和非入侵植物生长、生物量分配及氮获取的影响存在显著差异, 而这种生长策略的差异有利于入侵植物地上部分的快速生长与扩张, 从而提高其入侵性。

关键词: 入侵植物, 无菌培养体系, 氮, 生长策略, 新生境扩张

Abstract:

Aims Many factors can influence the invasiveness of alien plants, and among them endophytes may play a key role. Thus, the aim of this study is to investigate the effects of endophytic nitrogen-fixing bacteria on the growth strategy of invasive plants.

Methods We grew the invasive plant Sphagneticola trilobata and its native congener S. calendulacea infected by endophytic nitrogen-fixing bacteria or not under two nitrogen levels (low and high) and compared their growth and total nitrogen content.

Important findings The endophytic bacteria Kosakonia sp. WTB-JS007, isolated from S. trilobata, had different effects on the growth strategy of the two species (S. trilobata and S. calendulacea) and such an effect did not depend on the nitrogen levels. Under the low nitrogen level, inoculation with WTB-JS007 showed no significant effect on the growth or total nitrogen content of S. calendulacea, but significantly increased aboveground biomass (by 30.48%), promoted stolon length, decreased belowground biomass (by 56.58%), and enhanced total nitrogen content (by 47.51%) of S. trilobata. Similarly, under the high nitrogen level, endophytic bacteria stimulated the aboveground growth of S. trilobata, but had no effect on that of S. calendulacea. These results suggest that endophytic nitrogen-fixing bacteria can differently affect the growth, biomass allocation and nitrogen uptake of invasive and its co-occurring native plant species. Such a difference in the growth strategy can facilitate the rapid growth and expansion of the aboveground part of invasive plants and thus promote their invasiveness.

Key words: invasive plant, aseptic culture system, nitrogen, growth strategy, new habitat expansion

汪晶晶, 王嘉浩, 黄致云, Vanessa Chiamaka OKECHUKW, 胡蝶, 祁珊珊, 戴志聪, 杜道林. 不同氮水平下内生固氮菌对入侵植物南美蟛蜞菊生长策略的影响. 植物生态学报, 2023, 47(2): 195-205. DOI: 10.17521/cjpe.2022.0004

WANG Jing-Jing, WANG Jia-Hao, HUANG Zhi-Yun, Vanessa Chiamaka OKECHUKW, HU Die, QI Shan-Shan, DAI Zhi-Cong, DU Dao-Lin. Effects of endophytic nitrogen-fixing bacteria on the growth strategy of an invasive plant Sphagneticola trilobata under different nitrogen levels. Chinese Journal of Plant Ecology, 2023, 47(2): 195-205. DOI: 10.17521/cjpe.2022.0004

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2022.0004

https://www.plant-ecology.com/CN/Y2023/V47/I2/195

图/表 5

图1 固氮菌菌株WTB-JS007的鉴定。A, WTB-JS007在LB平板上的菌落形态。B, WTB-JS007扫描电镜图。C, 基于16S rRNA基因序列的WTB-JS007系统进化树。D, 用固氮引物(F1-nifH-3r)进行PCR扩增后的凝胶电泳图。E, 基于固氮引物扩增的固氮基因序列的WTB-JS007系统发育树。CK, 对照; Marker, 标记。

Fig. 1 Identification of nitrogen-fixing bacteria strain WTB-JS007. A, Colony morphology of WTB-JS007 on LB plate. B, Scanning electronic microscopy (SEM) of WTB-JS007. C, Phylogenetic tree of WTB-JS007 based on 16S rRNA gene sequence. D, Nitrogen fixation primer (F1-nifH-3r) PCR amplified gel electrophoresis. E, Phylogenetic tree of WTB-JS007 based on nitrogen fixation gene on the sequence amplified with nitrogen fixation primers. CK, control.

图2 南美蟛蜞菊和蟛蜞菊在低氮(Low-N)和正常氮(Nor-N)处理下不加菌(CK)或加菌(WTB-JS007)的叶面积(A)、茎长(B)、根数(C)、根长(D) (平均值±标准误, n = 4)。不同小写字母表示南美蟛蜞菊不同处理间存在显著差异(p < 0.05); 不同大写字母表示蟛蜞菊不同处理间存在显著差异(p < 0.05)。

Fig. 2 Leaf area (A), stem length (B), number of roots (C) and root length (D) of Sphagneticola trilobata and S. calendulacea without (CK) or with nitrogen-fixing bacteria (WTB-JS007) under low (Low-N) and normal (Nor-N) nitrogen (mean ± SE, n = 4). Different lowercase letters indicate significant differences (p < 0.05) among different treatments of S. trilobata; different uppercase letters indicate significant differences (p < 0.05) among different treatments of S. calendulacea.

图3 南美蟛蜞菊和蟛蜞菊在低氮(Low-N)和正常氮(Nor-N)处理下不加菌(CK)或加菌(WTB-JS007)的地上生物量(A)、地下生物量(B)、比叶面积(C)、根冠比(D) (平均值±标准误, n = 4)。不同小写字母表示南美蟛蜞菊不同处理间存在显著差异(p < 0.05); 不同大写字母表示蟛蜞菊不同处理间存在显著差异(p < 0.05)。

Fig. 3 Aboveground biomass (A), belowground biomass (B), specific leaf area (C) and root:shoot (D) of Sphagneticola trilobata and S. calendulacea without (CK) or with nitrogen-fixing bacteria (WTB-JS007) under low (Low-N) and normal (Nor-N) nitrogen (mean ± SE, n = 4). Different lowercase letters indicate significant differences (p < 0.05) among different treatments of S. trilobata; different uppercase letters indicate significant differences (p < 0.05) among different treatments of S. calendulacea.

图4 不同氮(低氮(Low-N)、正常氮(Nor-N))处理及不接种(CK)、接种内生固氮菌(WTB-JS007)处理下的南美蟛蜞菊和蟛蜞菊叶片氮含量(平均值±标准误, n = 4)。不同小写字母表示在低氮环境下不同处理间存在显著差异(p < 0.05); 不同大写字母表示在正常氮环境下不同处理间存在显著差异(p < 0.05)。

Fig. 4 Nitrogen content of Sphagneticola trilobata and S. calendulacea without (CK) or with nitrogen-fixing bacteria (WTB-JS007) under low (Low-N) and normal (Nor-N) nitrogen (mean ± SE, n = 4). Different lowercase letters indicate significant differences (p < 0.05) among different treatments under the low-nitrogen level; different uppercase letters indicate significant differences (p < 0.05) among different treatments under the normal nitrogen level.

图5 南美蟛蜞菊在内生固氮菌定植情况下生长策略的变化。

Fig. 5 Changes of the growth strategy of Sphagneticola trilobata under the colonization of endophytic nitrogen-fixing bacteria.

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不同氮水平下内生固氮菌对入侵植物南美蟛蜞菊生长策略的影响

Effects of endophytic nitrogen-fixing bacteria on the growth strategy of an invasive plant Sphagneticola trilobata under different nitrogen levels

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