植物生态学报 ›› 2011, Vol. 35 ›› Issue (5): 531-538.DOI: 10.3724/SP.J.1258.2011.00531
所属专题: 生物多样性
张利锐, 彭艳玲, 任广朋, 周永锋, 李忠虎, 刘建全*()
收稿日期:
2010-08-03
接受日期:
2010-12-13
出版日期:
2011-08-03
发布日期:
2011-06-07
通讯作者:
刘建全
作者简介:
* E-mail: liujq@nnwipb.ac.cn
ZHANG Li-Rui, PENG Yan-Ling, REN Guang-Peng, ZHOU Yong-Feng, LI Zhong-Hu, LIU Jian-Quan*()
Received:
2010-08-03
Accepted:
2010-12-13
Online:
2011-08-03
Published:
2011-06-07
Contact:
LIU Jian-Quan
摘要:
利用两个核基因座位C3H和GI, 对重叠分布于中国东南部的两个松属(Pinus)物种马尾松(P. massoniana)和黄山松(P. hwangshanensis)的22个群体88个个体进行了遗传多样性和种间分化模式研究。在这两个核基因座位上, 两种植物都表现出较低的核苷酸多样性水平(马尾松πsil = 0.001 71; 黄山松πsil = 0.003 40), 但是马尾松要显著低于黄山松; 在种内分化水平上, 马尾松的种内遗传分化也明显低于黄山松(马尾松FST = 0.059; 黄山松FST = 0.339)。这可能是由于黄山松的海拔分布高于马尾松, 而高海拔分布使黄山松的分布区域更加片段化, 促使其形成较高的种内遗传多样性和遗传分化。分子变异分析(AMOVA)发现, 两物种基于两个核基因座位的种间差异为48.86%, 而GI基因座位上的种间差异明显高于C3H座位(GI: 77.24%, C3H: 20.48%), 同时, 基因谱系显示两物种的共享单倍型仅在C3H座位上存在。结合这两个基因的功能, 推测GI基因可能在物种形成过程中受到了一定的选择压力, 因为GI基因参与调控植物的开花时间, 而C3H与木质素表达水平的调控有关。不同的选择压力使得GI的进化速度相对较快, 从而加速了黄山松和马尾松的物种分化。
张利锐, 彭艳玲, 任广朋, 周永锋, 李忠虎, 刘建全. 马尾松和黄山松两个核基因位点的群体遗传多样性和种间分化. 植物生态学报, 2011, 35(5): 531-538. DOI: 10.3724/SP.J.1258.2011.00531
ZHANG Li-Rui, PENG Yan-Ling, REN Guang-Peng, ZHOU Yong-Feng, LI Zhong-Hu, LIU Jian-Quan. Population genetic diversity and species divergence of Pinus massoniana and P. hwangshanensis at two nucleotide loci. Chinese Journal of Plant Ecology, 2011, 35(5): 531-538. DOI: 10.3724/SP.J.1258.2011.00531
图1 马尾松和黄山松群体采样地点分布(A)和核基因座位C3H和GI的基因谱系(B, C)。
Fig. 1 Sampling localities of Pinus massoniana and P. hwangshanensis (A) and gene genealogies of nuclear loci C3H and GI (B, C).
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表2 马尾松和黄山松核基因座位C3H和GI核苷酸多样性和中性检测
Table 2 Nucleotide diversity and neutrality tests over the C3H and GI locus in Pinus massoniana and P. hwangshanensis
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变异来源 Source of variation (%) | 遗传分化系数 Coefficient of gene differentiation (FST) | ||||
---|---|---|---|---|---|
基因座位 Gene locus | 种间 Among species | 种内群体间 Among populations within species | 群体内 Within population | 马尾松 P. massoniana | 黄山松 P. hwangshanensis |
C3H | 20.48 | 15.81 | 63.71 | 0.071 | 0.210* |
GI | 77.24 | 6.99 | 15.77 | 0.047 | 0.467** |
平均值 Average | 48.86 | 11.35 | 39.74 | 0.059 | 0.339 |
表3 马尾松和黄山松核基因座位C3H和GI变异的分子方差(AMOVA)分析(10 000次重复)
Table 3 Analysis of molecular variance (AMOVA) of nuclear loci C3H and GI variation in Pinus massoniana and P. hwangshanensis (10 000 permutations)
变异来源 Source of variation (%) | 遗传分化系数 Coefficient of gene differentiation (FST) | ||||
---|---|---|---|---|---|
基因座位 Gene locus | 种间 Among species | 种内群体间 Among populations within species | 群体内 Within population | 马尾松 P. massoniana | 黄山松 P. hwangshanensis |
C3H | 20.48 | 15.81 | 63.71 | 0.071 | 0.210* |
GI | 77.24 | 6.99 | 15.77 | 0.047 | 0.467** |
平均值 Average | 48.86 | 11.35 | 39.74 | 0.059 | 0.339 |
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