3,4-二羟基苯乙酮胁迫对天山云杉种子萌发过程中内源植物激素含量变化的影响

doi:10.3724/SP.J.1258.2013.00114

摘要/Abstract

摘要：

3,4-二羟基苯乙酮(DHAP)是天山云杉(Picea schrenkiana ssp. tianschanica)叶和凋落物中存在的主要自毒物质, 是导致天山云杉林天然更新障碍的原因之一。为了解释自毒物质发生作用的生理机制, 该文设计多个浓度梯度的DHAP溶液处理天山云杉种子, 以发芽率和发芽势为种子萌发参数, 运用反相超高效液相色谱(UPLC)分析技术, 检测了种子萌发过程中内源植物激素玉米素(ZT)、赤霉素(GA₃)、吲哚乙酸(IAA)和脱落酸(ABA)含量水平的变化。研究结果表明: DHAP处理对天山云杉种子萌发影响具有浓度效应, 表现为5.0 mmol·L^-1 > 0.1 mmol·L^-1 > 1.0 mmol·L^-1 >对照, 即5.0 mmol·L^-1 DHAP处理组对种子萌发的抑制作用最强、0.1 mmol·L^-1 DHAP处理组次之、1.0 mmol·L^-1 DHAP处理组最弱; DHAP处理组的种子内源ZT、GA₃浓度水平降低, ABA含量升高, GA₃浓度峰值出现时间延迟, IAA浓度在高浓度(5.0 mmol·L^-1 DHAP)处理组短时间内(3-6天)过量积累, 1.0和5.0 mmol·L^-1 DHAP处理组的种子内源ABA浓度峰值出现时间延迟; DHAP处理种子萌发1-6天时, ZT/(GA₃+IAA)比值降低, IAA/ZT、ABA/ZT比值增大; ABA/(ZT + GA₃ + IAA)比值在0.1 mmol·L^-1 DHAP处理组增大, 在5.0 mmol·L^-1 DHAP处理组降低。DHAP处理引发种子内源激素含量水平及激素含量间比值变化, 可能是抑制、延迟天山云杉种子萌发的主要原因。

关键词: 3,4-二羟基苯乙酮(DHAP), 内源植物激素, 天山云杉, 种子萌发, 超高效液相色谱(UPLC)

Abstract:

Aims Picea schrenkiana ssp. tianschanica is a major tree species in forest ecosystems in the Xinjiang Uygur Autonomous Region, West China, and plays an important role in the regional water conservation. 3,4-dihydroxy acetophenone (DHAP) is a principal autotoxic substance occurring in foliage and litter of P. schrenkiana ssp. tianschanica that causes suppressed natural regeneration in P. schrenkiana ssp. tianschanica forests. The objectives of this study were to investigate changes in the content of endogenous plant hormones during seed germination in P. schrenkiana ssp. tianschanica as affected by DHAP, and to elucidate the underlying physiological mechanisms in the autotoxic effects.
Methods We investigated the inter-relationships among DHAP treatment, seed germination, and changes in the concentration of endogenous plant hormones by measuring the contents of zeatin (ZT), gibberellin (GA₃), indole-acetic acid (IAA), and abscisic acid (ABA) during germination process in P. schrenkiana ssp. tianschanica seeds with or without DHAP treatment. The concentrations of plant hormones in germinating seeds were measured with the method of ultra performance liquid chromatography (UPLC).
Important findings Results showed that the impact of DHAP on seed germination in P. schrenkiana ssp. tianschanica depended on treatment concentrations, in the order of 5.0 mmol·L^-1 > 0.1 mmol·L^-1 > 1.0 mmol·L^-1 > control; treatments with DHAP reduced the concentrations of ZT and GA₃, delayed the peak occurrence of GA₃, and increased the concentration of ABA with a delayed peak occurrence under treatments of 1.0 and 5.0 mmol·L^-1 DHAP. Treatment with 5.0 mmol·L^-1 DHAP for 3-6 days greatly increased the level of IAA. 1-6 days of treatments with DHAP reduced the ZT/(GA₃ + IAA) ratio, and increased the IAA/ZT ratio and ABA/ZT ratio; the ABA/(ZT + GA₃ + IAA) ratio increased at 0.1 mmol·L^-1 DHAP and decreased at 5.0 mmol·L^-1 DHAP. It is suggested that DHAP treatment causes changes in the content and balance of endogenous plant hormones, leading to inhibition or delaying in seed germination in P. schrenkiana ssp. tianschanica.

Key words: 3,4-dihydroxy acetophenone (DHAP), endogenous plant hormone, Picea schrenkiana ssp. tianschanina, seed germination, ultra performance liquid chromatography (UPLC)

陈志颖, 阮晓, 张玉竹, 潘存德, 王强. 3,4-二羟基苯乙酮胁迫对天山云杉种子萌发过程中内源植物激素含量变化的影响. 植物生态学报, 2013, 37(12): 1114-1122. DOI: 10.3724/SP.J.1258.2013.00114

CHEN Zhi-Ying, RUAN Xiao, ZHANG Yu-Zhu, PAN Cun-De, WANG Qiang. Effects of 3,4-dihydroxy acetophenone stress on changes in the content of endogenous plant hormones during seed germination in Picea schrenkiana ssp. tianschanica. Chinese Journal of Plant Ecology, 2013, 37(12): 1114-1122. DOI: 10.3724/SP.J.1258.2013.00114

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2013.00114

https://www.plant-ecology.com/CN/Y2013/V37/I12/1114

图/表 4

图1 4种植物激素和3,4-二羟基苯乙酮(DHAP)的超高效液相色谱(UPLC)图。A, 标准品色图谱。B, 样品色图谱。ABA, 脱落酸; GA3, 赤霉素; IAA, 吲哚乙酸; ZT, 玉米素。

Fig. 1 Chromatogram of four plant hormones and 3,4-dihydroxy acetophenone (DHAP) by ultra performance liquid chromatography (UPLC). A, Standard chromatogram. B, Sample chromatogram. ABA, abscisic acid; GA3, gibberellin; IAA, indoleacetic acid; ZT, zeatin.

图2 3,4-二羟基苯乙酮(DHAP)处理对天山云杉种子萌发的影响。种子用DHAP处理后第7天为发芽势(平均值±标准偏差, n = 3)。不同小写字母表示同一处理时间不同浓度DHAP处理之间差异显著( p < 0.05)。

Fig. 2 Effects of 3,4-dihydroxy acetophenone (DHAP) on seed germination in Picea schrenkiana ssp. tianschanica. Germination vigor was assessed on the 7th day following treatment with DHAP (mean ± SD, n = 3). Different lower-case letters indicate significant differences among DHAP concentrations at the same treatment time (p < 0.05).

图3 3,4-二羟基苯乙酮(DHAP)处理对天山云杉种子萌发过程中内源激素相对含量变化的影响(平均值±标准偏差, n = 3)。A, 玉米素(ZT)。B, 赤霉素(GA3)。C, 吲哚乙酸(IAA)。D, 脱落酸(ABA)。

Fig. 3 Effects of 3,4-dihydroxy acetophenone (DHAP) on the concentration of endogenous plant hormones during seed germination in Picea schrenkiana ssp. tianschanica (mean ± SD, n = 3). A, Zeatin (ZT). B, Gibberellin (GA3). C, Indoleacetic acid (IAA). D, Abscisic acid (ABA).

图4 3,4-二羟基苯乙酮(DHAP)处理对天山云杉种子萌发过程中内源激素玉米素(ZT)、赤霉素(GA3)、吲哚乙酸(IAA)、脱落酸(ABA)间比值的影响(平均值±标准偏差, n = 3)。

Fig. 4 Effects of 3,4-dihydroxy acetophenone (DHAP) on the ratio among the endogenous plant hormones zeatin (ZT), gibberellin (GA3), indoleacetic acid (IAA), and abscisic acid (ABA) during seed germination of Picea schrenkiana ssp. tianschanica (mean ± SD, n = 3).

参考文献 37

[1]	Anosheh HP, Emam Y, Pessarakli M (2013). Changes in endogenous hormonal status in corn (Zea mays) hybrids under drought stress. Journal of Plant Nutrition, 36, 1695-1707. DOI URL
[2]	Baydar H (2002). Effects of gibberellic acid treatment for pollen sterility induction on the physiological activity and endogenous hormone levels of the seed in safflower. Turkish Journal of Biology, 26, 235-239.
[3]	Broz AK, Broeckling CD, De-la-Peña C, Lewis MR, Greene E, Callaway RM, Sumner LW, Vivanco JM (2010). Plant neighbor identity influences plant biochemistry and physiology related to defense. BMC Plant Biology, 10, 115. DOI URL PMID
[4]	Chon SU, Nelson CJ (2010). Allelopathy in compositae plants: a review. Agronomy for Sustainable Development, 30, 349-358. DOI URL
[5]	Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010). Abscisic acid: emergence of a core signaling network. Annual Review of Plant Biology, 61, 651-679. DOI URL PMID
[6]	Davière JM, Achard P (2013). Gibberellin signaling in plants. Development, 140, 1147-1151. DOI URL
[7]	Davies PJ (2010). Plant Hormones: Biosynthesis, Signal Transduction, Action. Kluwer Academic Publishers, London. 27-28.
[8]	Depuydt S, Hardtke CS (2011). Hormone signalling crosstalk in plant growth regulation. Current Biology, 21, 365-373.
[9]	Fernandez C, Voiriot S, Mévy JP, Vila B, Ormeño E, Dupouyet S, Bousquet-Mélou A (2008). Regeneration failure of Pinus halepensis Mill.: the role of autotoxicity and some abiotic environmental parameters. Forest Ecology and Management, 255, 2928-2936. DOI URL
[10]	Hegde RS, Miller DA (1990). Allelopathy and autotoxicity in alfalfa: characterization and effects of preceding crops and residue incorporation. Crop Science, 30, 1255-1259. DOI URL
[11]	Hutchison CE, Kieber JJ (2002). Cytokinin signaling in Arabidopsis. Plant and Cell, 14, S47-S59. DOI URL
[12]	Inderjit , Weston LA, Duke SO (2005). Challenges, achievements and opportunities in allelopathy research. Journal of Plant Interactions, 1, 69-81. DOI URL
[13]	Kato-Noguchi H, Seki T, Shigemori H (2010). Allelopathy and allelopathic substance in the moss Rhynchostegium pallidifolium. Journal of Plant Physiology, 167, 468-471. DOI URL PMID
[14]	Kaya Y, Aksakal Ö, Sunar S, Erturk FA, Bozari S, Agar G, Erez ME, Battal P (2012). Phytotoxical effect of Lepidium draba L. extracts on the germination and growth of monocot (Zea mays L.) and dicot (Amaranthus retroflexus L.) seeds. Toxicology and Industrial Health. http://tih.sagepub.com/content/early/2013/01/03/0748233712471702. Cited on May 27, 2013. DOI URL PMID
[15]	Kohli A, Sreenivasulu N, Lakshmanan P, Kumar PP (2013). The phytohormone crosstalk paradigm takes center stage in understanding how plants respond to abiotic stresses. Plant Cell Reports, 32, 945-957. DOI URL
[16]	Lara-Nunez A, Sánchez-Nieto S, Anaya AL (2009). Phytotoxic effects of Sicyos deppei (Cucurbitaceae) in germinating tomato seeds. Physiologia Plantarum, 136, 180-192. DOI URL PMID
[17]	Li ZH, Wang Q, Ruan X, Pan CD, Jiang DA (2010). Phenolics and plant allelopathy. Molecules, 15, 8933-8952. DOI URL PMID
[18]	Lin WX (2009). Effect of Casuarina equisetifolia autotoxicity on endogerous hormones. Chinese Agricultural Science Bulletin, 25(19), 100-103. (in Chinese with English abstract)
	[ 林武星 (2009). 木麻黄自毒作用物对其幼苗内源激素的影响. 中国农学通报, 25(19), 100-103.]
[19]	Lopez MA, Bannenberg G, Castresana C (2008). Controlling hormone signaling is a plant and pathogen challenge for growth and survival. Current Opinion in Plant Biology, 11, 420-427. DOI URL
[20]	Maqbool N, Wahid A, Farooq M, Cheema ZA, Siddique KHM (2013). Allelopathy and abiotic stress interaction in crop plants. Allelopathy, 451-468.
[21]	Muscolo A, Sidari M (2006). Seasonal fluctuations in soil phenolics of a coniferous forest: effects on seed germination of different coniferous species. Plant and Soil, 284, 305-318. DOI URL
[22]	Peleg Z, Blumwald E (2011). Hormone balance and abiotic stress tolerance in crop plants. Current Opinion in Plant Biology, 14, 290-295. DOI URL
[23]	Perilli S, Moubayidin L, Sabatini S (2010). The molecular basis of cytokinin function. Current Opinion in Plant Biology, 13, 21-26. DOI URL
[24]	Prasad MNV, Devi SR (2002). Physiological basis for allelochemical action of ferulic acid. In: Reigosa MJ, Pedrol N eds. Allelopathy: From Molecular to Ecosystems. Science Publishers, Enfield, USA. 25-43.
[25]	Qin D, Wang JZ, Guo JM, Zhai H (2009). The relation between endogenous hormones and late-germination in buds of Avrolles apple. Agricultural Sciences in China, 8, 564-571. DOI URL
[26]	Rice EL (2009). Allelopathy. 2nd edn. Academic Press, New York.
[27]	Ruan X, Li ZH, Wang Q, Pan CD, Jiang DA, Wang GG (2011). Autotoxicity and allelopathy of 3, 4-dihydroxy- acetophenone isolated from Picea schrenkiana Needles. Molecules, 16, 8874-8893. DOI URL
[28]	Singh HP, Batish DR, Kohli RK (1999). Autotoxicity: concept, organisms, and ecological significance. Critical Reviews in Plant Sciences, 18, 757-772. DOI URL
[29]	Sreenivasulu N, Harshavardhan VT, Govind G, Seiler C, Kohli A (2012). Contrapuntal role of ABA: Does it mediate stress tolerance or plant growth retardation under long-term drought stress? Gene, 506, 265-273. DOI URL
[30]	Wang Q, Ruan X, Pan CD, Xu NY, Luo X, Huang MM (2006). Need for sustainability policy―a case study of the Natural Forest Conservation Program (NFCP) in the western region of Tianshan Mountain, China. The Forestry Chronicle, 82, 31-39. DOI URL
[31]	Wang Q, Ruan X, Yan QC (2005). Study on the effect of plant hormones and pre-chilled treatment to break dormancy and germination of Rhodiola rosea seeds. Journal of Zhejiang University (Agriculture and Life Sciences), 31, 423-432.
[32]	Xu XY, Fan R, Zheng R, Li CM, Yu DY (2011). Proteomic analysis of seed germination under salt stress in soybeans. Journal of Zhejiang University-Science B (Biomedicine & Biotechnology), 12, 507-517.
[33]	Yang GQ, Wan FH, Liu WX, Guo JY (2008). Influence of two allelochemicals from Ageratina adenophora Sprengel on ABA, IAA and ZR contents in roots of upland rice seedlings. Allelopathy Journal, 21, 253-262.
[34]	Zeng RS, Mallik AU, Luo SM (2008). Allelopathy in Sustainable Agriculture and Forestry. Springer, New York. 363-386.
[35]	Zhang DJ, Zhang J, Yang WQ, Wu FZ (2010). Potential allelopathic effect of Eucalyptus grandis across a range of plantation ages. Ecological Research, 25, 13-23. DOI URL
[36]	Zhang XS, Zhang YS (1963). Preliminary study on the natural regeneration of Picea schrenkiana in Wusu forest. Xinjiang Agricultural Sciences, (1), 8-12 (in Chinese)
	[ 张新时, 张瑛山 (1963). 乌苏林区天山云杉天然更新的初步研究. 新疆农业科学, (1), 8-12.]
[37]	Zhao YD (2010). Auxin biosynthesis and its role in plant development. Annual Review of Plant Biology, 61, 49-64. DOI URL PMID