短葶飞蓬黄酮及咖啡酸酯的含量与土壤氮供应量的关系

doi:10.3773/j.issn.1005-264x.2009.05.007

摘要/Abstract

摘要：

通过对短葶飞蓬(Erigeron breviscapus)不同产地的44个种群植株的总黄酮、灯盏乙素和总咖啡酸酯含量与产地土壤全氮(N)和速效N含量的相关分析, 探讨了产地间土壤N含量变化在药用植物短葶飞蓬植株次生代谢有效成分含量变化中的作用机制。结果显示: 土壤全N含量与种群植株中总黄酮含量呈显著的负相关(r=-0.391, p＜0.01), 而与总咖啡酸酯和灯盏乙素关系不显著; 土壤速效N含量与种群植株中总黄酮(r=-0.528, p＜0.01)、灯盏乙素(r=-0.490, p＜0.01)和总咖啡酸酯含量(r=-0.471, p＜0.01)都表现出更高的极显著负相关关系。全部44个种群中, 有效N供应量变化只能解释次生代谢产物含量的17%~30%的变化。非石灰土壤与石灰土壤的种群分开进行线性回归, 可解释总黄酮含量产地间约80%的变化, 以及灯盏乙素和总咖啡酸酯含量约60%的变化。植株总黄酮、灯盏乙素和总咖啡酸酯含量都与植株含N量存在显著的负相关关系。研究表明: 土壤丰富的有效N供应不利于黄酮和咖啡酸酯的合成积累, 与“碳素/营养平衡假说”的预测一致; 土壤有效N对植物次生代谢产物的影响会受到植物体内外因素的影响。不同产地的短葶飞蓬的黄酮等酚类次生代谢产物含量的差异, 可能是产地间不同的土壤N供应量和其他生态因子及遗传变异共同作用的结果。

关键词: 灯盏花, 土壤氮, 次生代谢产物, 黄酮, 咖啡酸酯

Abstract:

Aims Our objectives are to explore the effect of nitrogen availability in soil on the accumulation of the secondary metabolites of plants growing in different areas, to test the C/N hypothesis, and to address reasons for the variability of content of active constituents in medicinal herbs growing in different areas.
Methods We surveyed plants and soils in 44 populations of a medicinal plant, Erigeron breviscapus. We analyzed total and active nitrogen of soil samples and total flavonoids, scutellarin, caffeates and nitrogen in plant samples. Linear regression analysis was used to study relationships between soil nitrogen availability and active constituents of plants.
Important findings The contents of flavonoids and caffeates in plants were negatively correlated with soil nitrogen availability and plant nitrogen content, as predicted by the carbon/nutrient balance hypothesis. Variation in available soil nitrogen accounted for 17%-30% of the variation in the content of secondary metabolites of plants. In contrast, variation in available nitrogen accounted for about 80% of the variation in content of total flavonoids in the rendizina and about 60% of content of scutellarin and caffeates in the non-rendizina. It appeared that effects of nitrogen availability on the accumulation of secondary metabolites in the plant were affected by other factors, such as genetic and environmental variation. The content of flavonoids and caffeates in plants growing in different areas varied with differences in nitrogen availability, ecological factors and genotypes.

Key words: Erigeron breviscapus, soil nitrogen, secondary metabolite, flavonoids, caffeates

苏文华, 张光飞, 周鸿, 张亚妮. 短葶飞蓬黄酮及咖啡酸酯的含量与土壤氮供应量的关系. 植物生态学报, 2009, 33(5): 885-892. DOI: 10.3773/j.issn.1005-264x.2009.05.007

SU Wen-Hua, ZHANG Guang-Fei, ZHOU Hong, ZHANG Ya-Ni. EFFECTS OF NITROGEN AVAILABILITY IN SOIL ON THE CONTENTS OF FLAVONOIDS AND CAFFEATES IN A MEDICINAL PLANT, ERIGERON BREVISCAPUS. Chinese Journal of Plant Ecology, 2009, 33(5): 885-892. DOI: 10.3773/j.issn.1005-264x.2009.05.007

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.05.007

https://www.plant-ecology.com/CN/Y2009/V33/I5/885

图/表 7

图1 分布区内所有土壤全N含量与短葶飞蓬植株总黄酮含量的回归分析

Fig. 1 Linear regression of the total flavonoid content in Ergieron breviscapus against the total N content in the soil

图2 非黑色石灰土(a)和黑色石灰土(b)全N含量与短葶飞蓬总黄酮含量的回归分析示意图

Fig. 2 Linear regression of the total flavonoid content in Ergieron breviscapus against the total N content in the non-rendizina (a) or rendizina (b)

图3 非黑色石灰土中全N含量与短葶飞蓬总咖啡酸酯和灯盏乙素含量的回归分析 ▲总咖啡酸酯 Total caffeates △灯盏乙素 Scutellarin

Fig. 3 Linear regression of the active constituent content in Ergieron breviscapus against the total N content in the non-rendizina

图4 短葶飞蓬整个分布区生境土壤碱解N含量与植株总黄酮含量的线性回归分析

Fig. 4 Linear regression of the total flavonoid content in the plant of Ergieron breviscapus against the active N content in the soil

图5 短葶飞蓬非黑色石灰土(a)和黑色石灰土(b)生境土壤碱解N含量与植株总黄酮含量的线性回归分析

Fig. 5 Linear regression of the total flavonoid content in Ergieron breviscapus against the active N content in the non-rendizina (a) or rendizina (b)

图6 非黑色石灰土(a)和黑色石灰土(b)土壤速效N与短葶飞蓬有效成分含量的回归分析 ▲总咖啡酸酯 Total caffeates △ 灯盏乙素 Scutellarin

Fig. 6 Linear regression of the active constituent content in Ergieron breviscapus against the active N content in the non-rendizina (a) or rendizina (b)

图7 短葶飞蓬植株总黄酮含量与含N量的回归分析

Fig. 7 Linear regression of the total flavonoid content against the N content in Ergieron breviscapus

参考文献 36

[1]	Bao SD (鲍士旦) (2005). Soil Agrochemistry Analysis (土壤农化分析) 3rd edn. China Agriculture Press, Beijing, 187. (in Chinese)
[2]	Bazza FS, Nona RC, Coley PD, Pitelka LF (1987). Allocating resources to reproduction and defense. BioScience, 37, 53-67.
[3]	Bryant JP, Chapin FS, Klein DR (1983). Carbon/nutrient balance of boreal plants in relation to vertebrate herbivory. Oikos, 40, 357-368. DOI URL
[4]	Chinese Pharmacopoeia Commission (国家药典委员会) (2005). Chinese Pharmacopoeia (中华人民共和国药典) 1st edn. Chemical Industry Press, Beijing, 447-448. (in Chinese)
[5]	Close DC, McArthur C (2002). Rethinking the role of many plant phenolics-protection from photodamage not herbivores. Oikos, 99, 166-172. DOI URL
[6]	Coley PD, Brant JP, Chapin FS (1985). Resource availability and plant antiherbivore defense. Science, 230, 895-899. DOI URL PMID
[7]	Foyer CH, Lelandais M, Kunert KJ (1994). Photooxidative stress in plants. Physiologia Plantarum, 63, 257-264.
[8]	Guidi L, Lorefice G, Pardossi A, Malorgio F, Tognoni F, Soldatini GF (1998). Growth and photosynthesis of Lycopersicon esculentum(L.) plants as affected by nitrogen deficiency. Biologia Plantarum, 40, 235-244.
[9]	Hamilton JG, Zangerl AR, DeLucia EH, Berenbaum MR (2001). The carbon-nutrient balance hypothesis: its rise and fall. Ecology Letters, 4, 86-95. DOI URL
[10]	Herms DA, Masttson WJ (1992). The dilemma of plants: to grow or to defend. Quarterly Review of Biology, 67, 283-335. DOI URL
[11]	Huang LQ (黄璐琦), Zhang RX (张瑞贤) (1997). Biological study on Geo-herbs. Chinese Pharmaceutical Journal (中国药学杂志), 32, 563-566. (in Chinese)
[12]	Jiang XH (蒋向辉), Wu XJ (伍贤进), Huang XQ (黄星群), Zhang J (张俭), Wei L (魏麟), She CW (佘朝文) (2007). Effect of different fertilizer and application rate on flavonoids content in the subterranean stem of Houttuynia cordata Thunb. Hubei Agricultural Sciences (湖北农业科学), 46(2), 215-217. (in Chinese with English abstract)
[13]	Kong CH (孔垂华), Hu F (胡飞) (2001). Plant Allelopathy and Its Application (植物化感相生相克作用及其应用). China Agriculture Press, Beijing, 168-181, 202-215. (in Chinese)
[14]	Kong CH (孔垂华), Xu T (徐涛), Hu F (胡飞), Huang SS (黄寿山) (2000). Allelopathy under environmental stress and its induced mechanism. Acta Ecologica Sinica (生态学报), 20, 849-854. (in Chinese with English abstract)
[15]	Koricheva J (2002). The carbon-nutrient balance hypothesis is dead; long live the carbon-nutrient balance hypothesis? Oikos, 98, 537-539. DOI URL
[16]	Kovacik J, Klejdus B, Backor M, Repcak M (2007). Phenylalanine ammonia-lyase activity and phenolic compounds accumulation in nitrogen-deficient Matricaria chamomilla leaf rosettes. Plant Science, 172, 393-399. DOI URL
[17]	Ma YH (马宇辉), Lou GA (罗国安), Wang YM (王义明) (2004). Advances in studies on Erigeron breviscapus. Chinese Traditional Patent Medicine (中成药), 26(1), 63-65. (in Chinese)
[18]	Margna U (1977). Control at the level of substrate supply— an alternative in the regulation of phenylpropanoid accumulation in plant cells. Phytochemistry, 16, 419-426. DOI URL
[19]	Martin LC, Eric P, Donald FC (2002). Effect of nitrogen and water treatment on leaf chemistry in horsenettle (Solanum carolinenes), and relationship to herbivory by flea beetles (Epitrix spp.) and tobacco hornworm (Manduca sexta). Journal of Chemical Ecology, 28, 2377-2398. DOI URL
[20]	McArthur C, Marsh NR, Close DC, Walsh A, Paterson S, Fitzgerald H, Davies NW (2003). Nursery conditions affect seedling chemistry, morphology and herbivore preference for Eucalyptus nitens. Forest Ecology and Management, 176, 585-594. DOI URL
[21]	Montanari M, Degl’Innocenti E, Maggini R, Pacifici S, Pardossi A, Guidi L (2008). Effect of nitrate fertilization and saline stress on the contents of active constituents ofEchinacea gustifolia DC. Food Chemistry, 107, 1461-1466. DOI URL
[22]	Moore BD, Wallis IR, Wood JT, Foley WJ (2004). Foliar nutrition, site quality, and temperature influence foliar chemistry of Tallowwood (Eucalyptus microcorys). Ecological Monographs, 74, 553-568. DOI URL
[23]	Nitao JK, Zangerl AR, Berenbaum MR (2002). CNB: requiescat in pace? Oikos, 98, 540-546. DOI URL
[24]	Niyogi KK (1999). Photoprotection revisited: genetic and molecular approaches. Annual Review of Plant Physiology and Plant Molecular Biology, 50, 333-359. DOI URL PMID
[25]	Pare PW, Tumlinson JH (1997). Induced synthesis of plant volatiles. Nature, 385, 30-31. DOI URL
[26]	Rice-Evans CA, Miller NJ, Paganga G (1996). Structure- antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology & Medicine, 20, 933-956. DOI URL PMID
[27]	Stewart AJ, Chapman W, Jenkins GI, Graham I, Martin T, Crozier A (2001). The effect of nitrogen and phosphorus deficiency on flavonol accumulation in plant tissues. Plant, Cell and Environment, 24, 1189-1197.
[28]	Sudha G, Ravishankar GA (2002). Involvement and interaction of various signaling compounds on the plant metabolic events during defense response, resistance to stress factors formation of secondary metabolites and their molecular aspects. Plant Cell, Tissue and Organ Culture, 71, 181-212.
[29]	Sugiharto B, Miyata K, Nakamoto H (1990). Regulation of expression of carbon-assimilating enzymes by nitrogen in maize leaf. Plant Physiology, 92, 963-969. DOI URL PMID
[30]	Su WH (苏文华), Lu J (陆洁), Zhang GF (张光飞), Wang CY (王崇云) (2001). Ecological and biological analysis of total flavonoids in Erigeron breviscapus. Chinese Traditional and Herbal Drugs (中草药), 32, 1119-1121. (in Chinese with English abstract)
[31]	Tan SC (1980). Phenylalanine ammonia-lyase and the phenylalanine ammonia-lyase inactivating system: effects of light, temperature and mineral deficiences. Australian Journal of Plant Physiology, 7, 159-167.
[32]	Terashima I, Evans JR (1988). Effect of light and nitrogen nutrition on the organisation of the photosynthetic apparatus in spinach. Plant & Cell Physiology, 29, 143-155.
[33]	Xu HQ (许海琴), Xu LQ (许列琴), Xu JW (许江苇) (2003). A Handbook for Referential Quality Standards of Natural Extractives (常用天然提取物质量标准参考手册). Chemical Industry Press, Modern Bio- technology and Medical Science and Technology Centre Beijing,, 124-125. (in Chinese)
[34]	Zang XY (臧小云), Liu LP (刘丽萍), Cai QS (蔡庆生) (2006). Effect of nitrogen supply on flavone and rutin accumulation in stems and leaves of common buckwheat (Fagopyrum esculentum Moench). Journal of Nanjing Agricultural University (南京农业大学学报), 29(3), 28-32. (in Chinese with English abstract)
[35]	Zhang Y (张燕), Liu Y (刘勇), Wang WQ (王文全), Wang JY (王继永) (2007). Effects of N P K on yield and baicalin contents of Scutellaria baicalensis. Journal of Chinese Medicinal Materials (中药材), 30, 286-387. (in Chinese with English abstract)
[36]	Zhou J (周洁), Zhang YQ (张永清) (2006). A partial study on Geo-herbs. Journal of Shandong University of Traditional Chinese Medicine (山东中医药大学学报), 30, 252-254. (in Chinese)