植物生态学报 ›› 2013, Vol. 37 ›› Issue (5): 397-406.doi: 10.3724/SP.J.1258.2013.00041

• 研究论文 • 上一篇    下一篇

中亚热带森林土壤微生物群落多样性随海拔梯度的变化

吴则焰1,2, 林文雄2*, 陈志芳3, 方长旬2, 张志兴2, 吴林坤2, 周明明2, 陈婷2   

  1. 1福建农林大学生命科学学院, 福州 350002;
    2福建农林大学农业生态研究所, 福州 350002;
    3福建农林大学林学院, 福州 350002
  • 收稿日期:2013-01-08 修回日期:2013-04-19 出版日期:2013-05-01 发布日期:2013-05-16
  • 通讯作者: 林文雄 E-mail:wenxiong1810@163.com
  • 基金资助:

    教育部博士点基金项目;福建省教育厅资助省属高校项目

Variations of soil microbial community diversity along an elevational gradient in mid-subtropical forest

WU Ze-Yan1,2, LIN Wen-Xiong2*, CHEN Zhi-Fang3, FANG Chang-Xun2, ZHANG Zhi-Xing2, WU Lin-Kun2, ZHOU Ming-Ming2, and CHEN Ting2   

  1. 1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

    2Institute of Agroecology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;

    3College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2013-01-08 Revised:2013-04-19 Online:2013-05-01 Published:2013-05-16
  • Contact: LIN Wen-Xiong E-mail:wenxiong1810@163.com

摘要:

运用Biolog EcoPlate技术, 对武夷山不同海拔植被带(常绿阔叶林(EBF)、针叶林(CF)、亚高山矮林(DF)、高山草甸(AM))土壤微生物群落多样性差异进行了研究。结果表明: 不同海拔植被带土壤微生物群落功能多样性差异显著。土壤平均颜色变化率(AWCD)随培养时间延长而逐渐增加, 同一深度土层的AWCD值随海拔升高而逐渐降低, 大小顺序依次为EFB > CF > DF > AM。同一海拔植被带, 不同深度土层的AWCD值总体趋势依次为0–10 cm > 10–25 cm > 25–40 cm。土壤微生物群落Simpson指数、Shannon-Wiener指数、丰富度指数和McIntosh指数的总体趋势为EBF最高, CF和DF次之, AM最低。不同海拔植被带土壤微生物对不同碳源利用强度存在较大差异, 其中EBF利用率最高, AM利用率最低, 碳水化合物和羧酸类碳源是各海拔植被带土壤微生物的主要碳源。主成分分析结果表明, 从31个因素中提取的与碳源利用相关的主成分1、主成分2分别能解释变量方差的75.27%和16.14%, 在主成分分离中起主要贡献作用的是胺类和氨基酸类碳源。土壤微生物群落多样性随着海拔上升、土层加深而逐渐下降的原因, 可能是生物量、林分凋落物、土壤养分、微小动物、植物根系等多种因素共同作用的结果。

Abstract:
Aims Soil microbes play a key role in material recycling and energy conversion in forest ecosystems. We studied the functional diversity of microbial communities and soil physical-chemical properties in order to illustrate variations of soil microbial community diversity along an elevational gradient in the Wuyi Mountains of China and influencing factors.
Methods Evergreen broad-leaved forest (EBF), coniferous forest (CF), dwarf forest (DF) and alpine meadow (AM) were chosen as experimental sites along an elevation gradient in the National Natural Reserve of the Wuyi Mountains. We used Biolog EcoPlate analysis to study the functional diversity of microbial communities.
Important findings There were significant differences in functional diversity of microbial communities in different soil elevations. The average well color development (AWCD), which directly reflected microbial activity and functional diversity, increased over time, while AWCD at the same soil depth gradually decreased along with increasing altitude and in the order of EBF > CF > DF > AM. The AWCD of different soil depths for the same vegetation type was in the order of 0–10 cm > 10–25 cm > 25–40 cm. The Simpson index, Shannon-Wiener index, richness index and McIntosh index of EBF were all higher than AM. There was variation among substrates used by soil microbial communities in different soil elevations. The substrate used in EBF was significantly higher than the substrate used in AM. The carbon sources mostly used by soil microbes were carbohydrates and carboxylic acids, followed by amino acids, phenolic acids and polymers; the lowest was amines. Principal component analysis (PCA) identified two factors in relation to carbon sources; they explained 75.27% and 16.14% of the variation. The carbon sources used by soil microbial communities were different among different vegetation types. Amino acids and amides were the two main carbon sources separating the two principal component factors. Soil microbial community diversity decreased along the elevation gradient and with soil thickness, and vegetative biomass, forest litter, soil nutrient, microzoon and plant roots might be the major factors controlling this diversity.