狮牙草状风毛菊果期资源分配对海拔的响应

doi:10.17521/cjpe.2020.0201

摘要/Abstract

摘要：

植物资源分配是目前植物生态学研究的热点问题, 主要集中在性分配和繁殖分配两个方面。该研究以分布在青藏高原的狮牙草状风毛菊(Saussurea leontodontoides)作为研究材料, 研究了6个海拔高度上果期植株的繁殖特征及资源分配的差异, 并用异速模型分析了繁殖性状及资源分配与个体大小的关系。结果显示: 1)狮牙草状风毛菊的个体大小、繁殖器官生物量、营养器官生物量、种子数和营养分配均与海拔存在极显著负相关关系, 百粒质量和繁殖分配与海拔存在极显著正相关关系。2)在不同海拔高度下, 百粒质量、种子数、繁殖器官生物量及营养分配与植株个体大小呈正相关关系; 繁殖分配与植株个体大小呈负相关关系; 而营养器官生物量与植株个体大小呈极显著正相关关系。这表明海拔和个体大小对狮牙草状风毛菊的繁殖对策有不同程度的影响, 狮牙草状风毛菊通过增加繁殖部分的生物量和百粒质量来适应高海拔的胁迫环境。

关键词: 海拔, 狮牙草状风毛菊, 异速模型, 繁殖分配

Abstract:

Aims Our objective is to study the differences between the reproduction characteristics and resource allocation of Saussurea leontodontoides in the fruiting stage across six levels of the elevation gradient, and analyze the relationship between the above two parameters and individual size of S. leontodontoides.
Methods The study site is located in the northeastern margin of the Qingzang Plateau. The collection and investigation were carried out on the fruiting individuals of S. leontodontoides which were distributed in an elevation range of 3 200-4 540 m. 40 complete individuals were randomly collected from each level of the elevation. The number of seeds in each capitulum was counted, then the biomass was measured after drying at 80 °C in the oven. The differences between the reproduction characteristics and resource allocation of S. leontodontoides at different levels of the elevation gradient were analyzed by ANOVA. The relationship between the reproduction characteristics with individual size, resource allocation with individual size at different levels of the elevation gradient was analyzed by the allometric model.
Important findings Our results showed that: 1) The individual size, reproductive organ biomass, vegetative organ biomass, seed number and vegetative allocation were negatively correlated with elevation; the hundred grain mass and reproductive allocation were positively correlated with elevation. 2) Along the elevation gradient, the hundred grain mass, the seed number, the reproductive organs biomass and the vegetative allocation were positively correlated with individual size; reproductive allocation was negatively related to individual size; and vegetative organ biomass and individual size were significantly positively correlated. This indicates that the elevation and individual size had different effects on the reproductive strategy of S. leontodontoides. Our study suggests that S. leontodontoides adapted to the high-elevation stress environment by increasing the reproductive organs biomass and the hundred grain mass.

Key words: elevation, Saussurea leontodontoides, allometric model, reproductive allocation

李蕾, 王一峰, 苟文霞, 马文梅, 蒋春玲. 狮牙草状风毛菊果期资源分配对海拔的响应. 植物生态学报, 2020, 44(11): 1164-1171. DOI: 10.17521/cjpe.2020.0201

LI Lei, WANG Yi-Feng, GOU Wen-Xia, MA Wen-Mei, JIANG Chun-Ling. Response of resource allocation of Saussurea leontodontoides during its fruiting stage to the elevation. Chinese Journal of Plant Ecology, 2020, 44(11): 1164-1171. DOI: 10.17521/cjpe.2020.0201

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链接本文: https://www.plant-ecology.com/CN/10.17521/cjpe.2020.0201

https://www.plant-ecology.com/CN/Y2020/V44/I11/1164

图/表 6

参考文献 40

[1]	Cao GX, Yan J, Li Y, Ji P (2007). Intra-inflorescence variation in female fitness components and seed size-number trade-off in Hosta plantaginea (Liliaceae). Journal of Southwest University (Natural Science Edition), 29, 147-150.
	[ 操国兴, 严娟, 李燕, 季萍 (2007). 玉簪花序水平上雌性适合度成分变化及种子数量与大小权衡关系研究. 西南大学学报(自然科学版), 29, 147-150.]
[2]	Cao GX, Zhong ZC, Xie DT, Liu Y (2005). The relationship between reproductive allocation, fruit set and individual size of Camellia rosthorniana in different communities. Acta Phytoecologica Sinica, 29, 261-266.
	[ 操国兴, 钟章成, 谢德体, 刘芸 (2005). 不同群落中川鄂连蕊茶的生殖分配与个体大小之间关系的探讨. 植物生态学报, 29, 261-266.]
[3]	Chen K, Yang Y, Xu L, Liu X (2020). Comparison of seed trade-off strategies among Cardiocrinum giganteum populations along an elevationinal gradient in Gaoligong Mountains. Chinese Journal of Ecology, 39, 3231-3236.
	[ 陈凯, 杨艳, 徐玲, 刘霞 (2020). 高黎贡山不同海拔大百合种子权衡策略的比较. 生态学杂志, 39, 3231-3236.]
[4]	Chen T, Wang YF (2014). Response of reproductive characteristics of Saussurea macrota Franch. to elevation at eastern Qinhai-Tibetan Plateau. Chinese Journal of Ecology, 33, 3216-3221.
	[ 陈亭, 王一峰 (2014). 青藏高原东缘大耳叶风毛菊繁殖特征对海拔的响应. 生态学杂志, 33, 3216-3221.]
[5]	Chen XL, Liang Y, Qi W, Su M, Du GZ (2009). Studies on the reproductive allocation, floral size and its trade-off with floral number of annual Gentiana. Acta Prataculturae Sinica, 18, 58-66.
	[ 陈学林, 梁艳, 齐威, 苏梅, 杜国祯 (2009). 一年生龙胆属植物的繁殖分配及其花大小、数量的权衡关系研究. 草业学报, 18, 58-66.]
[6]	Feener M (1985). Seed Ecology. Chapman and Hall, London. URL PMID
[7]	Gross KL (1984). Effects of seed size and growth form on seedling establishment of six monocarpic perennial plants. Journal of Ecology, 72, 369-387.
[8]	Hou QZ, Wen J, Li HC, Ren YL, Sha JH, Zhao DG (2017). Reproductive allocation of three Gentianaceae species. Pratacultural Science, 34, 1017-1023.
	[ 侯勤正, 文静, 李昊聪, 任昱兰, 沙建华, 赵东光 (2017). 3种龙胆科植物的资源分配对策. 草业科学, 34, 1017-1023.]
[9]	Hou QZ, Ye GJ, Ma XB, Su X, Zhang SH, Sun K (2016). Size-dependent reproductive allocation of Gentianopsis paludosa in different habitats of the Qinghai-Tibetan Plateau. Acta Ecologica Sinica, 36, 2686-2694.
	[ 侯勤正, 叶广继, 马小兵, 苏雪, 张世虎, 孙坤 (2016). 青藏高原不同生境下湿生扁蕾(Gentianopsis paludosa)个体大小依赖的繁殖分配. 生态学报, 36, 2686-2694.]
[10]	Jin GL (2009). Study on the Plant Ecological Adaption Strategy of Degraded Seriphidium transiliense Desert Grassland. PhD dissertation, Xinjiang Agricultural University, Ürümqi.
	[ 靳瑰丽 (2009). 伊犁绢蒿荒漠退化草地植物生态适应对策的研究. 博士学位论文, 新疆农业大学, 乌鲁木齐.]
[11]	Korner C (2009). Functional Plant Ecology of High Mountain Ecosystems. Science Press, Beijing.
[12]	Lebrija-Trejos E, Reich PB, Hernández A, Wright SJ (2016). Species with greater seed mass are more tolerant of conspecific neighbours: a key driver of early survival and future abundances in a tropical forest. Ecology Letters, 19, 1071-1080. URL PMID
[13]	Leishman MR, Murray BR (2001). The relationship between seed size and abundance in plant communities: model predictions and observed patterns. Oikos, 94, 151-161.
[14]	Leishman MR, Wright IJ, Moles AT (2000). The evolutionary ecology of seed size. Trends in Ecology & Evolution, 7, 368-372. URL PMID
[15]	Li GQ, Zhao PP, Shao WS, Jin CQ, Song LX, Chen YY (2019). Effect of enclosure on reproductive allocation of wheatgrass Agropyron mongolicum populations in desert steppes. Ecology and Evolution, 9, 14023-14030. URL PMID
[16]	Liu ZJ, Du GZ, Chen JK (2002). Size-dependent reproductive allocation of Ligularia virgaurea in different habitats. Chinese Journal of Plant Ecology, 26, 44-50.
	[ 刘左军, 杜国祯, 陈家宽 (2002). 不同生境下黄帚橐吾(Ligularia virgaurea)个体大小依赖的繁殖分配. 植物生态学报, 26, 44-50.]
[17]	Mao WY, Wang YF, Yang LL, Song WD, Ma WM (2019). Reproductive allocation and floral characteristic of Saussurea katochaete in relation to elevation. Chinese Journal of Ecology, 38, 60-66.
	[ 毛婉嫕, 王一峰, 杨励龙, 宋卫东, 马文梅 (2019). 重齿风毛菊繁殖分配及花部特征与海拔的相关性. 生态学杂志, 38, 60-66.]
[18]	Milla R, Giménez-Benavides L, Escudero A, Reich PB (2009). Intra- and interspecific performance in growth and reproduction increase with altitude: a case study with two Saxifraga species from northern Spain. Functional Ecology, 23, 111-118.
[19]	Moles AT, Westoby M (2004). What do seedlings die from and what are the implications for evolution of seed size? Oikos, 106, 193-199. DOI URL
[20]	Morse DH, Schmitt J (1985). Propagule size, dispersal ability, and seedling performance in Asclepias syriaca. Oecologia, 67, 372-379. URL PMID
[21]	Qi H, Duan LS, Wang SL, Wang Y, Zhang Q, Feng GY, Du HY, Liang QL, Lin YZ (2017). Effect of enhanced UV-B radiation on cotton growth and photosynthesis. Chinese Journal of Eco-Agriculture, 25, 708-719.
	[ 祁虹, 段留生, 王树林, 王燕, 张谦, 冯国艺, 杜海英, 梁青龙, 林永增 (2017). 全生育期UV-B辐射增强对棉花生长及光合作用的影响. 中国生态农业学报, 25, 708-719.]
[22]	Qi RL, Ma WM, Qi BY, Cao JH, Yang YJ, Ma HR, Wang YF (2019). Altitudinal variation of floral organs in Saussurea przewalskii and its relationship with the number and mass of seeds. Chinese Journal of Applied Ecology, 30, 2647-2653. URL PMID
	[ 祁如林, 马文梅, 祁百元, 曹家豪, 杨亚军, 马海荣, 王一峰 (2019). 弯齿风毛菊花部器官的海拔变异及其与种子质量和数目的关系. 应用生态学报, 30, 2647-2653.] PMID
[23]	Sakai A, Sasa A, Sakai S (2006). Do sexual dimorphisms in reproductive allocation and new shoot biomass increase with an increase of altitude? A case of the shrub willow Salix reinii (Salicaceae). American Journal of Botany, 93, 988-992. URL PMID
[24]	Shi Z, Jin SY (1999). Flora of China: Vol. 78. Book 2. Science Press, Beijing. 191.
	[ 石铸, 靳淑英 (1999). 中国植物志: 第78卷. 第2分册. 科学出版社, 北京. 191.]
[25]	Sonamtso, Wang YF, Li M, Yue YC, Li MX (2013). Reproductive allocation of Saussurea hieracioides (Asteraceae), a common species in eastern margin of Qinghai-Tibet Plateau. Chinese Journal of Ecology, 32, 1433-1438.
	[ 索南措, 王一峰, 李梅, 岳永成, 李毛先 (2013). 青藏高原东缘常见种长毛风毛菊(Saussurea hieracioides)的繁殖分配. 生态学杂志, 32, 1433-1438.]
[26]	Tamagno S, Sadras VO, Ortez OA, Ciampitti IA (2020). Allometric analysis reveals enhanced reproductive allocation in historical set of soybean varieties. Field Crops Research, 248, 107717. DOI: 10.1016/j.fcr.2020.107717.
[27]	Wang HX (2012). Mechanisms of Plant Leaves Based on Leaf Surface Wettability on Rainfall Interception and Dust- capturing. PhD dissertation, Xi’an University of Architecture and Technology, Xi’an.
	[ 王会霞 (2012). 基于润湿性的植物叶面截留降水和降尘的机制研究. 博士学位论文, 西安建筑科技大学, 西安.]
[28]	Wang SY (2017). Response of Seed Storage Substances, Germination and Seedlings of 7 Dominant Gramineous Plants in Alpine Meadow to Grassland N and P Addition. Master degree dissertation, Lanzhou University, Lanzhou.
	[ 王素玉 (2017). 高寒草甸7种优势禾本科植物种子贮藏物质、萌发及幼苗对草地N、P添加的响应. 硕士学位论文, 兰州大学, 兰州.]
[29]	Wang TH (2006). The Study of Plant Phenotypic Plasticity and Life-History Strategies. PhD dissertation, Northeast Normal University, Changchun.
	[ 王天慧 (2006). 植物表型可塑性及生活史对策研究. 博士学位论文, 东北师范大学, 长春.]
[30]	Wang YF (2009). Studies on Ecology and Biology of the Genus of Saussurae (Compositae) in the East of Qinghai-Tibetan Plateau. PhD dissertation, Lanzhou University, Lanzhou.
	[ 王一峰 (2009). 青藏高原东缘风毛菊属植物生态与生物学研究. 博士学位论文, 兰州大学, 兰州.]
[31]	Wang YF, Jin J, Cao JH, Hou HH, Li XJ (2016). The response of Saussurea dzeurensis fruiting resource allocation to differences in elevation. Acta Ecologica Sinica, 36, 5790-5797.
	[ 王一峰, 靳洁, 曹家豪, 侯宏红, 李筱姣 (2016). 川西风毛菊果期资源分配对海拔的响应. 生态学报, 36, 5790-5797.]
[32]	Wang YF, Kou J, Wang WY, Ma YX, Li YY (2017). Variation of fruiting resource allocation along elevations in Saussurea kansuensis. Journal of Northwest Normal University (Natural Science), 53, 87-91.
	[ 王一峰, 寇靖, 王文越, 马诣欣, 李怡颖 (2017). 甘肃风毛菊果期资源分配对海拔的响应. 西北师范大学学报(自然科学版), 53, 87-91.]
[33]	Wang YF, Li M, Li SX, Guo J, Chen YP, Wang RX (2012). Variation of reproductive allocation along elevations in Saussurea stella on East Qinghai-Xizang Plateau. Chinese Journal of Plant Ecology, 36, 1145-1153.
	[ 王一峰, 李梅, 李世雄, 郭杰, 陈玉萍, 王瑞雪 (2012). 青藏高原东缘星状风毛菊生殖分配对海拔的响应. 植物生态学报, 36, 1145-1153.]
[34]	Wang YF, Yue YC (2014). Effects of resource allocation and floral traits on the number and mass of Saussurea undulata seeds from different elevations in eastern Qinghai-Xizang Plateau. Chinese Journal of Plant Ecology, 38, 366-374.
	[ 王一峰, 岳永成 (2014). 青藏高原东缘不同海拔波缘风毛菊资源分配及花部特征对种子数目和质量的影响. 植物生态学报, 38, 366-374.]
[35]	Webb CJ, Shand JE (1985). Reproductive biology of tree lucerne (Chamaecytisus palmensis, Leguminosae). New Zealand Journal of Botany, 23, 597-606.
[36]	Weiner J, Campbell LG, Pino J, Echarte L (2009). The allometry of reproduction within plant populations. Journal of Ecology, 97, 1220-1233.
[37]	Wilson AM, Thompson K (1989). A comparative study of reproductive allocation in 40 British grasses. Functional Ecology, 3, 297-302.
[38]	Zhang C, An YM, Jäschke Y, Wang LL, Zhou ZL, Wang LP, Yang YP, Duan YW (2020). Processes on reproductive ecology of plant species in the Qinghai-Xizang Plateau and adjacent highlands. Chinese Journal of Plant Ecology, 44, 1-21.
	[ 张婵, 安宇梦, Jäschke Y, 王林林, 周知里, 王力平, 杨永平, 段元文 (2020). 青藏高原及周边高山地区的植物繁殖生态学研究进展. 植物生态学报, 44, 1-21.]
[39]	Zhang DY (2000). Researches on Theoretical Ecology. Springer Press, Beijing.
	[ 张大勇 (2000). 理论生态学研究. 施普林格出版社, 北京.]
[40]	Zhao ZG, Du GZ, Ren QJ (2004). Size-dependent reproduction and sex allocation in five species of Ranunculaceae. Acta Phytoecologica Sinica, 28, 9-16.
	[ 赵志刚, 杜国祯, 任青吉 (2004). 5种毛茛科植物个体大小依赖的繁殖分配和性分配. 植物生态学报, 28, 9-16.]

种群数量 Population number	采集时间 Collection date	海拔 Altitude (m)	经纬度 Latitude and longitude	采样地 Sampling site	生境类型 Habitat Type	样本数 Sample size
1	2018-08-26	3 200	102.89° E, 34.91° N	合作市电视塔山 TV Tower Mountain in Hezuo	山坡草甸 Hillside meadow	40
1	2017-08-30	3 490	101.11° E, 36.43° N	湟源县日月山 Riyue Mountain, Huangyuan	山坡草甸 Hillside meadow	40
1	2018-08-28	3 690	101.69° E, 33.78° N	玛曲县阿万仓山 Awancang Mountain, Maqu	山坡草甸 Hillside meadow	40
1	2017-08-31	3 970	99.44° E, 35.40° N	兴海县温泉乡 Wenquan Township, Xinghai	山坡草甸 Hillside meadow	40
1	2017-08-31	4 260	98.21° E, 34.91° N	玛多县城 Madoi County	山坡草甸 Hillside meadow	40
1	2017-08-12	4 540	92.73° E, 34.45° N	治多县109国道旁 Next to national highway 109 in Zhidoi	山坡草甸 Hillside meadow	40

种群数量 Population number	采集时间 Collection date	海拔 Altitude (m)	经纬度 Latitude and longitude	采样地 Sampling site	生境类型 Habitat Type	样本数 Sample size
1	2018-08-26	3 200	102.89° E, 34.91° N	合作市电视塔山 TV Tower Mountain in Hezuo	山坡草甸 Hillside meadow	40
1	2017-08-30	3 490	101.11° E, 36.43° N	湟源县日月山 Riyue Mountain, Huangyuan	山坡草甸 Hillside meadow	40
1	2018-08-28	3 690	101.69° E, 33.78° N	玛曲县阿万仓山 Awancang Mountain, Maqu	山坡草甸 Hillside meadow	40
1	2017-08-31	3 970	99.44° E, 35.40° N	兴海县温泉乡 Wenquan Township, Xinghai	山坡草甸 Hillside meadow	40
1	2017-08-31	4 260	98.21° E, 34.91° N	玛多县城 Madoi County	山坡草甸 Hillside meadow	40
1	2017-08-12	4 540	92.73° E, 34.45° N	治多县109国道旁 Next to national highway 109 in Zhidoi	山坡草甸 Hillside meadow	40

海拔 Altitude (m)	种子百粒质量 Hundred grain mass (g)	种子数 Seed number	繁殖器官生物量 Reproductive organ biomass (g)	营养器官生物量 Vegetative organ biomass (g)	繁殖分配 Reproductive allocation	营养分配 Vegetative allocation
3 200	y = -1.686 + 0.386x R²= 0.187, p = 0.004	y = 2.259 + 0.338x R²= 0.135, p = 0.200	y = -0.275 + 0.418x R²= 0.084, p = 0.066	y = -0.216 + 1.157x R²= 0.917, p < 0.01	y = -0.281 - 0.574x R²= 0.149, p = 0.014	y = -0.220 + 1.161x R²= 0.184, p = 0.006
3 490	y = -1.462 + 0.113x R²= 0.057, p = 0.126	y = 2.246 + 0.329x R²= 0.108, p = 0.040	y = -0.498 + 0.775x R²= 0.641, p < 0.01	y = -0.221 + 1.129x R²= 0.955, p < 0.01	y = -0.498 - 0.223x R²= 0.133, p = 0.021	y = -0.211 + 0.115x R²= 0.182, p = 0.004
3 690	y = -1.439 + 0.107x R²= 0.081, p = 0.072	y = 2.182 + 0.405x R²= 0.173, p = 0.009	y = -0.370 + 0.581x R²= 0.265, p = 0.001	y = -0.196 + 1.102x R²= 0.916, p < 0.01	y = -0.368 - 0.422x R²= 0.160, p = 0.011	y = -0.191 + 0.094x R²= 0.076, p = 0.070
3 970	y = -1.419 + 0.102x R²= 0.053, p = 0.154	y = 2.270 + 0.260x R²= 0.14, p = 0.015	y = -0.119 + 0.208x R²= 0.09, p = 0.064	y = -0.297 + 1.183x R²= 0.798, p = 0.010	y = -0.119 - 0.791x R²= 0.583, p < 0.01	y = -0.298 + 0.185x R²= 0.092, p = 0.054
4 260	y = -1.376 + 0.060x R²= 0.047, p = 0.185	y = 2.375 + 0.064x R²= 0.038, p = 0.196	y = -0.229 + 0.299x R²= 0.152, p = 0.013	y = -0.341 + 1.192x R²= 0.751, p < 0.01	y = -0.226 - 0.704x R²= 0.497, p < 0.01	y = -0.343 + 0.196x R²= 0.075, p = 0.091
4 540	y = -1.516 + 0.376x R²= 0.231, p = 0.003	y = 2.264 + 0.272x R²= 0.098, p = 0.049	y = -0.430 + 0.697x R²= 0.262, p = 0.001	y = -0.164 + 0.940x R²= 0.385, p < 0.01	y = -0.439 - 0.287x R²= 0.055, p = 0.144	y = -0.182 + 0.024x R²= 3.94E^-4, p = 0.840
F				B: 0.790^ns A: 9.886^**

海拔 Altitude (m)	种子百粒质量 Hundred grain mass (g)	种子数 Seed number	繁殖器官生物量 Reproductive organ biomass (g)	营养器官生物量 Vegetative organ biomass (g)	繁殖分配 Reproductive allocation	营养分配 Vegetative allocation
3 200	y = -1.686 + 0.386x R²= 0.187, p = 0.004	y = 2.259 + 0.338x R²= 0.135, p = 0.200	y = -0.275 + 0.418x R²= 0.084, p = 0.066	y = -0.216 + 1.157x R²= 0.917, p < 0.01	y = -0.281 - 0.574x R²= 0.149, p = 0.014	y = -0.220 + 1.161x R²= 0.184, p = 0.006
3 490	y = -1.462 + 0.113x R²= 0.057, p = 0.126	y = 2.246 + 0.329x R²= 0.108, p = 0.040	y = -0.498 + 0.775x R²= 0.641, p < 0.01	y = -0.221 + 1.129x R²= 0.955, p < 0.01	y = -0.498 - 0.223x R²= 0.133, p = 0.021	y = -0.211 + 0.115x R²= 0.182, p = 0.004
3 690	y = -1.439 + 0.107x R²= 0.081, p = 0.072	y = 2.182 + 0.405x R²= 0.173, p = 0.009	y = -0.370 + 0.581x R²= 0.265, p = 0.001	y = -0.196 + 1.102x R²= 0.916, p < 0.01	y = -0.368 - 0.422x R²= 0.160, p = 0.011	y = -0.191 + 0.094x R²= 0.076, p = 0.070
3 970	y = -1.419 + 0.102x R²= 0.053, p = 0.154	y = 2.270 + 0.260x R²= 0.14, p = 0.015	y = -0.119 + 0.208x R²= 0.09, p = 0.064	y = -0.297 + 1.183x R²= 0.798, p = 0.010	y = -0.119 - 0.791x R²= 0.583, p < 0.01	y = -0.298 + 0.185x R²= 0.092, p = 0.054
4 260	y = -1.376 + 0.060x R²= 0.047, p = 0.185	y = 2.375 + 0.064x R²= 0.038, p = 0.196	y = -0.229 + 0.299x R²= 0.152, p = 0.013	y = -0.341 + 1.192x R²= 0.751, p < 0.01	y = -0.226 - 0.704x R²= 0.497, p < 0.01	y = -0.343 + 0.196x R²= 0.075, p = 0.091
4 540	y = -1.516 + 0.376x R²= 0.231, p = 0.003	y = 2.264 + 0.272x R²= 0.098, p = 0.049	y = -0.430 + 0.697x R²= 0.262, p = 0.001	y = -0.164 + 0.940x R²= 0.385, p < 0.01	y = -0.439 - 0.287x R²= 0.055, p = 0.144	y = -0.182 + 0.024x R²= 3.94E^-4, p = 0.840
F				B: 0.790^ns A: 9.886^**