不同海拔青海云杉与祁连圆柏叶片抗氧化系统

doi:10.3773/j.issn.1005-264x.2009.04.019

摘要/Abstract

摘要：

以祁连山寺大隆林区连续海拔梯度(2 665~3 365 m)上青海云杉(Picea crassifolia)和祁连圆柏(Sabina przewalskii)为材料, 测定叶片中抗氧化保护系统的变化, 探讨常绿木本植物抗氧化系统对高山极端环境的适应机制。结果显示, 祁连圆柏和青海云杉叶片中丙二醛(MDA)含量均与海拔高度呈正相关, 相同海拔上青海云杉MDA含量极显著高于祁连圆柏(p＜0.01)。随海拔升高, 两树种抗氧化保护酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)活性和非酶促抗氧化剂脯氨酸(Pro)、抗坏血酸(AsA)、还原性谷胱甘肽(GSH)含量均呈明显增加趋势。青海云杉叶片的AsA水平高于祁连圆柏, 但对海拔变化的敏感性较低; 祁连圆柏的GSH、Pro水平及其对海拔变化的敏感性均高于青海云杉。结果表明, 研究区青海云杉所受过氧化伤害较祁连圆柏更严重, 但两树种清除O₂^-·的能力相当而主要负责分解H₂O₂的酶种有所不同: 祁连圆柏中为POD, 青海云杉中则为CAT、APX和GR, AsA-GSH循环系统在青海云杉活性氧清除中的作用强于在祁连圆柏中, 祁连圆柏的活性氧清除物质可能以Pro为主。

关键词: 青海云杉, 祁连圆柏, 海拔梯度, 抗氧化系统, 逆境适应性

Abstract:

Aims The antioxidative defense capacity of plants plays an important role in extreme environments. We investigated the antioxidative system in Picea crassifolia and Sabina przewalskiileaves along an altitudinal gradient in Sidalong forests of Qilian Mountains to study the response of antioxidative systems in evergreen xylophytes to subalpine environment.
Methods We determined the contents of malonaldehyde (MDA), praline (Pro), ascorbic acid (AsA) and glutathione (GSH), as well as the activities of superoxide dismutase (SOD) , peroxidase (POD) , catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) in leaves of P. crassifolia and S. przewalskii from different altitudes (2 665-3 365 m).
Important findings The MDA contents in P. crassifolia and S. przewalskii leaves positively and significantly correlated with altitude. At the same altitude, the MDA content in S. przewalskii leaves was significantly higher than that in P. crassifolia leaves. With increasing altitude, the content of Pro, AsA and GSH and the activities of SOD, POD, CAT, APX and GR in leaves of both P. crassifolia and S. przewalskii increased. The sensitivity of AsA, GSH and Pro in S. przewalskii leaves along the altitudinal gradient was higher than those in P. crassifolia, although the AsA content was lower than the later. Those results suggested that S. przewalskii suffered greater oxidative stress than P. crassifolia, but its capacity to cavenge O₂^-· was similar. POD was a main enzyme in S. przewalskii as decomposing H₂O₂, but P. crassifolia was CAT, APX and GR. The action of AsA-GSH circulatory system in P. crassifolia was greater than that in S. przewalskii, and Pro was the most important antioxidant substance in S. przewalskii.

Key words: Picea crassifolia, Sabina przewalskii, altitudinal gradient, antioxidative system, stress adaptation

张涛, 安黎哲, 陈拓, 代春艳, 陈年来. 不同海拔青海云杉与祁连圆柏叶片抗氧化系统. 植物生态学报, 2009, 33(4): 802-811. DOI: 10.3773/j.issn.1005-264x.2009.04.019

ZHANG Tao, AN Li-Zhe, CHEN Tuo, DAI Chun-Yan, CHEN Nian-Lai. ANTIOXIDATIVE SYSTEM IN LEAVES OF PICEA CRASSIFOLIA AND SABINA PRZEWALSKII ALONG AN ALTITUDINAL GRADIENT. Chinese Journal of Plant Ecology, 2009, 33(4): 802-811. DOI: 10.3773/j.issn.1005-264x.2009.04.019

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2009.04.019

https://www.plant-ecology.com/CN/Y2009/V33/I4/802

图/表 9

图1 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片丙二醛含量同一树种具有不同小写字母的海拔梯度间差异显著(p＜0.05) Lower case letter on bars of each species indicate significant difference at p＜0.05 level

Fig. 1 MDA contents in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图2 不同海拔高度青海云杉(空心)和祁连圆柏(实心)SOD活性图注同图1 Note see Fig. 1

Fig. 2 SOD activities in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图3 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片CAT活性图注同图1 Note see Fig. 1

Fig. 3 CAT activities in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图4 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片APX活性图注同图1 Note see Fig. 1

Fig. 4 APX activities in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图5 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片GR活性图注同图1 Note see Fig. 1

Fig. 5 GR Activities in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图6 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片POD活性图注同图1 Note see Fig. 1

Fig. 6 POD activities in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图7 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片抗坏血酸含量图注同图1 Note see Fig. 1

Fig. 7 Asa contents in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图8 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片谷胱甘肽含量图注同图1 Note see Fig. 1

Fig. 8 GSH contents in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

图9 不同海拔高度青海云杉(空心)和祁连圆柏(实心)叶片脯氨酸含量图注同图1 Note see Fig. 1

Fig. 9 Pro contents in leaves of Picea crassifolia (open) and Sabina przewalskii (solid) at different altitudes

参考文献 27

[1]	Bailly C, Benamar A, Corbineau F (1996). Changes in malondialdehyde content and in superoxide dismutase, actalase glutathione reductase activities in sunflower seeds as related to deterioration during accelerated aging. Physiologia Plantarum, 97, 104-110. DOI URL
[2]	Chen WN (陈文年), Wu N (吴宁), Luo P (罗鹏) (2005). Sabina przewalskii community structure in the upper reaches of Minjiang River. Chinese Journal of Applied Ecology (应用生态学报), 16, 197-202. (in Chinese with English abstract) URL PMID
[3]	Chen YP (陈银萍), Zhang MX (张满效), Chen T (陈拓), Zhang YF (张有富), An LZ (安黎哲) (2006). Seasonal changes of leaf antioxidative system in Sabina and their relations to freezing tolerance. Chinese Journal of Ecolog (生态学杂志), 25, 1318-1322. (in Chinese with English abstract)
[4]	Ellman GL (1959). Tissue eulfhydryl group. Arch Biochem Biophys, 82, 70-77. DOI URL PMID
[5]	Gechev T, Willekens H, Montagu MV, Inze D, Camp WV, Toneva V, Minkov I (2003). Different responses of tobacco antioxidant enzymes to light and chilling stress. Journal of Plant Physiology, 160, 509-515. URL PMID
[6]	Gong JR (龚吉蕊), Zhao AF (赵爱芬), Zhang LX (张立新), Zhang XS (张新时) (2004). A comparative study on antioxidative ability of several desert plants under drought stress. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 24, 1570-1577. (in Chinese with English abstract)
[7]	Han G (韩刚), Dang Q (党青), Zhao Z (赵忠) (2008). Response of antioxidation protection system of Hedysarum scoparium to drought stress. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 28, 1007-1013.
[8]	Kang HJ (康华靖), Liu P (刘鹏), Xu GD (徐根娣), Chen ZL (陈子林), Wei FM (韦福民) (2008). Ecophysiological response of Emmenopterys Henryi at different altitudes in China’s Dapan Mountain natural reserve. Journal of Plant Ecology (Chinese Version) (植物生态学报), 32, 865-872. (in Chinese with English abstract)
[9]	Knorzer OC, Durner J, Boger P (1996). Alterations in the antioxidative system of suspension-cultured soybean cells (Glycine max) induced by oxidative stress. Physiologia Plantarum, 97, 388-396. DOI URL
[10]	Liang JP (梁建萍), Niu Y (牛远), Xie JS (谢敬斯), Zhang JD (张建达) (2007). Antioxidase activities and photosynthetic pigment contents in Larix principis-rupprechtii leaves along an altitudinal gradient. Chinese Journal of Applied Ecology (应用生态学报), 18, 1414-1419. (in Chinese with English abstract)
[11]	Li HS (李合生) (2000). Principles and Techniques of Plant Physiological Biochemical Experiment (植物生理生化实验技术与方法). Higher Education Press, Beijing, 164-261. (in Chinese)
[12]	Li J (李晶), Yan XF (阎秀峰), Zu YG (祖元刚) (2000). Generation of activated oxygen and change of cell defense enzyme activity in leaves of Korean pine seedling under low temperature. Acta Botanica Sinica (植物学报), 42, 148-152. (in Chinese with English abstract)
[13]	Liu XC (刘兴聪) (1992). Picea crassifolia (青海云杉). Lanzhou University Press,Lanzhou. (in Chinese)
[14]	Lü L (吕琳), He CF (何聪芳), Dong YM (董银卯), Zhao H (赵华), Liu JX (刘家熙) (2006). Effects of environmental stress on ultra-structure of aloe. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 26, 1940-1945. (in Chinese with English abstract)
[15]	Ma XJ (马旭俊), Zhu DH (朱大海) (2003). Functional roles of the plant superoxide dismutase. Hereditas (遗传), 25, 225-231. (in Chinese with English abstract) URL PMID
[16]	Nakano Y, Asada K (1981). Hydrogen peroxide is scavenged by ascorbatespecific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22, 867-880.
[17]	Polle A, Rennenberg H (1992). Field studies on Norway spruce trees at high altitudes: II. Defence systems against oxidative stress in needles. New Phytologist, 121, 635-642. DOI URL
[18]	Sun XC (孙学成), Tan QL (谭启玲), Hu CX (胡承孝), Gan QQ (甘巧巧), Yi CC (易长城) (2006). Effects of molybdenum molybdenum on antioxidative enzymes in winter wheat under low temperature stress. Scientia Agricultura Sinica (中国农业科学), 39, 952-959. (in Chinese with English abstract)
[19]	Wang SY (王生耀), Wang K (王堃), Zhao YL (赵永来), Gong AQ (巩爱岐), Xin YJ (辛有俊) (2008). Effects of the drought and UV-B on the growth and anti-oxidative system of two perennial grasses. Acta Agrestia Sinica (草地学报), 16, 492-402. (in Chinese with English abstract)
[20]	Wang YB (王弋博), Feng HY (冯虎元), Qu Y (曲颖), Cheng JQ (程佳强), Wang XL (王勋陵), An LZ (安黎哲) (2007). Effects of reactive oxygen species on UV-B induced ethylene production in leaves of maize seedlings. Journal of Plant Ecology (Chinese Version)(植物生态学报), 31, 946-951. (in Chinese with English abstract)
[21]	Wildi B, Lütz C (1996). Antioxidant composition of selected high alpine plant species from different altitudes. Plant, Cell & Environment, 19, 138-146.
[22]	Wu JH (吴建慧), Yang L (杨玲), Sun GR (孙国荣) (2004). Generation of activated oxygen and chang of cell denfense enzyme activity in leaves of maize seedling under the stress of low temperzture. Bulletin of Botanical Research (植物研究), 24, 456-459. (in Chinese with English abstract)
[23]	Yang SS (杨淑慎), Gao JF (高俊凤) (2001). Influence of active oxygen and free radicals on plant senescence. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 21, 215-220. (in Chinese with English abstract)
[24]	Yi YQ (伊永强), Hu JB (胡建斌), Deng MJ (邓明军) (2007). Latest development of antioxidant system and responses to stress in plant leaves. Chinese Agricultural Science Bulletin (植物农学通报), 23, 105-110. (in Chinese with English abstract)
[25]	Zhao FY, Gou SL, Wang ZL, Zhao YX, Zhang H (2003). Recent advances in study on transgenic plants for salt tolerance. Journal of Plant Physiology and Molecular Biology, 29, 171-178.
[26]	Zhao LY (赵丽英), Deng XP (邓西平), Shan L (山仑) (2005). The response mechanism of active oxygen species removing system to drought stress. Acta Botanica Boreali-Occidentalia Sinica (西北植物学报), 25, 413-418. (in Chinese with English abstract)
[27]	Zou Q (邹琦) (2000). A Guide of Phytophysiological Experiment (植物生理学实验指导). China Agriculture Press，Beijing. (in Chinses)