大盘山自然保护区香果树对不同海拔生境的生理生态响应

doi:10.3773/j.issn.1005-264x.2008.04.015

摘要/Abstract

摘要：

在全面调查大盘山国家级自然保护区香果树(Emmenopterys henryi)分布的基础上, 设置了4个海拔段(A1: 550 ~ 650 m, A2: 680 ~ 770 m, A3: 810 ~ 900 m, A4: 970 ~ 1 100 m), 对不同海拔段内香果树的生理生态特性进行研究, 结果显示, 叶绿素a (Chla)、叶绿素b (Chlb)和总叶绿素(Chl(a+b))含量均随着海拔的上升而减小, 高海拔A4与低海拔A1相比, Chla、Chlb和Chl(a+b)含量分别下降了21.32%、31.53%和24.96%, 差异均达到显著水平。分析认为, 主要是由于相对较强的光照以及干旱胁迫的增强所致。同样, 比叶面积(SLA)也随着海拔的上升而减小, A4与A1相比下降了27.55%, 差异达到显著水平。丙二醛(MDA)含量和质膜透性(MP)变化较为一致, 两者均在A3处达到最低水平, 在A4处达到最高, 说明在A3受到的伤害最小而在A4受到的伤害最大; 脯氨酸(Pro)和抗坏血酸(AsA)含量先升高再降低, 在A3处则均达到最高, 与A1相比分别增加了139.33%和10.60%; 酶保护系统中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性在A1均最小, 随着海拔的升高, 其活性变化则不太一致: SOD活性一直增加, POD、CAT和APX活性虽然都是先增加后减小, 但POD和APX在A3达到最高, CAT则是在A2最高。非酶类保护物质含量的增加和酶活性的增强有利于清除细胞内的活性氧, 维持细胞膜的稳定性, 从而保证植物的正常生长。综合此次实验结果表明, 在中海拔(810~900 m)比较适合香果树的生长, 而高海拔(970~1 100 m)则不适合香果树的生长。

关键词: 香果树, 海拔, 抗氧化系统, 生理生态, 大盘山

Abstract:

Aims Emmenopterys henryi, an endemic species in China, is one of the Chinese national second class protective wild plants. We investigated the ecophysiological responses of E. henryi at different altitudes in Dapan Mountain, a National Nature Reserve in Eastern China, to determine ecophysiological adaptation mechanisms of the species antioxidative system.

MethodsWe studied the content of Chla、Chlb and Chl(a+b), malondialdehyde (MDA), ascorbate (AsA) and praline (Pro), the membrane permeability (MP), specific leaf area (SLA), and the activities of peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) and ascorbate peroxidase (APX) in leaves of E. henryi from different altitudes (A1: 550-650 m, A2: 680-770 m, A3: 810-900 m and A4: 970-1 100 m) in Dapan Mountain.

Important findingsChla, Chlb and Chl(a+b) reduced with increasing altitude. At A4, they decreased 21.32%, 31.53% and 24.96%, respectively, compared with that in A1. SLA decreased with increasing altitude. MP and content of MDA had a similar change: reaching minimum values in A3 and maximum in A4, indicating that E. henryi was least damaged in A3 and most in A4. Pro and AsA were at their maxima in A3, 139.33% and 10.60% respectively compared with A1. The activity of SOD, POD, CAT and APX were all the weakest in A1, however, they showed different changes. The activity of SOD progressively increased, while the activity of POD, CAT and APX initially increased and then decreased, with peak of activity of POD and APX in A3 and CAT in A2. More non-enzyme antioxidant and stronger activity of enzyme were favourable for eliminating intracellular active oxygen, keeping the cell membrane in a stable condition and ensuring normal growth of plants. In general, 810-900 m (middle altitude) is the best for the growth of E. henryi, while 970-1 100 m (high altitude) is the poorest. Therefore, altitude should be considered when biodiversity conservation of E. henryi is to be carried out, especially when transplanting E. henryi from degraded areas in the wild.

Key words: Emmenopterys henryi, altitude, antioxidative system, physioecological, Dapan Mountain

康华靖, 刘鹏, 徐根娣, 陈子林, 韦福民. 大盘山自然保护区香果树对不同海拔生境的生理生态响应. 植物生态学报, 2008, 32(4): 865-872. DOI: 10.3773/j.issn.1005-264x.2008.04.015

KANG Hua-Jing, LIU Peng, XU Gen-Di, CHEN Zi-Lin, WEI Fu-Min. ECOPHYSIOLOGICAL RESPONSE OF EMMENOPTERYS HENRYITO THE HABITATS IN DIFFERENT ALTITUDES IN CHINA'S DAPAN MOUNTAIN NATURAL RESERVE. Chinese Journal of Plant Ecology, 2008, 32(4): 865-872. DOI: 10.3773/j.issn.1005-264x.2008.04.015

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链接本文: https://www.plant-ecology.com/CN/10.3773/j.issn.1005-264x.2008.04.015

https://www.plant-ecology.com/CN/Y2008/V32/I4/865

图/表 4

图1 不同海拔对香果树叶片绿素含量和比叶面积的影响中各海拔间标有不同英文字母表明两者的差异显著水平(p<0.05) Different letters on the same index indicate significantly difference at p<0.05 level A1、A2、A3、A4: 海拔高度分别为550~650、680~770、810~900和970~1 100 m Denates altitude 550-650, 680-770, 810-900 and 970~1 100 m, respectively

Fig 1 The effect of different altitudes on the chlorophyll content and specific leaf area (SLA) of Emmenopterys henryi leaves

图2 不同海拔对香果树叶片MDA含量和质膜透性的影响图注同图1

Fig. 2 The effect of different altitudes on the MDA content and membrane permeability of Emmenopterys henryi leaves Notes see Fig. 1

图3 不同海拔对香果树叶片Pro和AsA含量的影响图注同图1

Fig. 3 The effect of different altitudes on the content of Pro and AsA of Emmenopterys henryi leaves Notes see Fig. 1

图4 不同海拔对香果树叶片抗氧化酶活性的影响图注同图1

Fig. 4 The effect of different altitudes on the activities of SOD, POD, CAT and APX of Emmenopterys henryi leaves Notes see Fig. 1

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