植物生态学报 ›› 2014, Vol. 38 ›› Issue (7): 749-756.DOI: 10.3724/SP.J.1258.2014.00070

• 研究论文 • 上一篇    下一篇

壳聚糖对红松幼苗多酚积累和抗氧化防御酶的诱导作用

刘冉, 王振宇*(), 李婷婷, 王芳, 安静   

  1. 东北林业大学林学院, 哈尔滨 150040
  • 收稿日期:2014-01-22 接受日期:2014-04-01 出版日期:2014-01-22 发布日期:2014-07-10
  • 通讯作者: 王振宇
  • 作者简介:* E-mail: wzy219001@163.com
  • 基金资助:
    国家自然科学基金(31170510)

The role of chitosan in polyphenols accumulation and induction of defense enzymes in Pinus koraiensis seedlings

LIU Ran, WANG Zhen-Yu*(), LI Ting-Ting, WANG Fang, AN Jing   

  1. College of Forestry, Northeast Forestry University, Harbin 150040, China
  • Received:2014-01-22 Accepted:2014-04-01 Online:2014-01-22 Published:2014-07-10
  • Contact: WANG Zhen-Yu

摘要:

为探究壳聚糖诱导促进红松(Pinus koraiensis)多酚合成的生理调控机制, 以红松幼苗为实验材料, 在DCR培养基中添加不同浓度的壳聚糖, 诱导8天后测定多酚和原花青素的含量, 筛选有利于多酚积累的最佳的壳聚糖浓度。随后测定最佳浓度壳聚糖诱导下红松幼苗中多酚物质积累量、防御酶活性和多酚合成途径关键酶活性随时间的变化。结果显示: 50-200 mg·L-1壳聚糖可以有效地提高多酚和原花青素的积累量。壳聚糖浓度为100 mg·L-1时诱导效果最佳, 多酚积累量可以达到(9.91 ± 0.68) mg·g -1鲜质量, 是对照组的1.64倍; 原花青素积累量可以达到(2.52 ± 0.11) mg·g -1鲜质量, 是对照组的1.53倍。100 mg·L-1壳聚糖诱导下红松幼苗防御相关酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)和多酚合成关键酶(苯丙氨酸转氨酶、肉桂酸4-羟化酶)迅速做出响应, 活性显著高于对照组。壳聚糖能够显著地激活红松幼苗的防御反应和苯丙烷代谢途径, 从而促进抗氧化物质多酚的合成与积累, 有利于提高红松幼苗的抵抗力。

关键词: 壳聚糖, 防御酶系, 苯丙烷代谢, 红松, 多酚

Abstract:

Aims This study aimed to explore the physiological mechanisms of polyphenols synthesis promoted by chitosan elicitor in Pinus koraiensis.
Methods Pinus koraiensis seedlings were cultured for eight days under different chitosan concentrations in the DCR media and the accumulations of polyphenols and proanthocyanidins were measured, in order to determine the chitosan concentration for optimal polyphenols accumulation. The changes in polyphenols accumulation, activities of defense enzymes, and activities of key enzymes for polyphenol synthesis were then studied in P. koraiensis seedlings under the optimal chitosan concentration.
Important findings Results showed that chitosan at the concentration ranging from 50 to 200 mg·L -1 effectively increased the accumulation of polyphenols and proanthocyanidins in P. koraiensis seedlings. The effect of induction by chitosan reached the peak at 100 mg·L -1. The polyphenols accumulation in P. koraiensis seedlings treated with chitosan reached (9.91 ± 0.68) mg·g -1 in fresh mass, which was 1.64 times the content in the control. The accumulation of proanthocyanidins reached (2.52 ± 0.11) mg·g -1 in fresh mass, which was 1.53 times the accumulation in the control. Activities of defense-related enzymes (superoxide dismutase, peroxidase, and catalase) and polyphenol biosynthesis-related enzymes (phenylalanine ammonia-lyse, and cinnamate-4-hydroxylase) in P. koraiensis seedlings were increased significantly by chitosan elicitor at the concentration of 100 mg·L -1. Chitosan could significantly activate defensive response and the phenylpropanoid pathway in P. koraiensis, thus promoting the synthesis and accumulation of polyphenols and enhancing the resistance of P. koraiensis seedlings.

Key words: chitosan, defence-related enzymes, phenylpropanoid pathway, Pinus koraiensis, polyphenols