植物生态学报 ›› 2014, Vol. 38 ›› Issue (7): 749-756.DOI: 10.3724/SP.J.1258.2014.00070
收稿日期:
2014-01-22
接受日期:
2014-04-01
出版日期:
2014-01-22
发布日期:
2014-07-10
通讯作者:
王振宇
作者简介:
* E-mail: wzy219001@163.com基金资助:
LIU Ran, WANG Zhen-Yu*(), LI Ting-Ting, WANG Fang, AN Jing
Received:
2014-01-22
Accepted:
2014-04-01
Online:
2014-01-22
Published:
2014-07-10
Contact:
WANG Zhen-Yu
摘要:
为探究壳聚糖诱导促进红松(Pinus koraiensis)多酚合成的生理调控机制, 以红松幼苗为实验材料, 在DCR培养基中添加不同浓度的壳聚糖, 诱导8天后测定多酚和原花青素的含量, 筛选有利于多酚积累的最佳的壳聚糖浓度。随后测定最佳浓度壳聚糖诱导下红松幼苗中多酚物质积累量、防御酶活性和多酚合成途径关键酶活性随时间的变化。结果显示: 50-200 mg·L-1壳聚糖可以有效地提高多酚和原花青素的积累量。壳聚糖浓度为100 mg·L-1时诱导效果最佳, 多酚积累量可以达到(9.91 ± 0.68) mg·g -1鲜质量, 是对照组的1.64倍; 原花青素积累量可以达到(2.52 ± 0.11) mg·g -1鲜质量, 是对照组的1.53倍。100 mg·L-1壳聚糖诱导下红松幼苗防御相关酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)和多酚合成关键酶(苯丙氨酸转氨酶、肉桂酸4-羟化酶)迅速做出响应, 活性显著高于对照组。壳聚糖能够显著地激活红松幼苗的防御反应和苯丙烷代谢途径, 从而促进抗氧化物质多酚的合成与积累, 有利于提高红松幼苗的抵抗力。
刘冉, 王振宇, 李婷婷, 王芳, 安静. 壳聚糖对红松幼苗多酚积累和抗氧化防御酶的诱导作用. 植物生态学报, 2014, 38(7): 749-756. DOI: 10.3724/SP.J.1258.2014.00070
LIU Ran, WANG Zhen-Yu, LI Ting-Ting, WANG Fang, AN Jing. The role of chitosan in polyphenols accumulation and induction of defense enzymes in Pinus koraiensis seedlings. Chinese Journal of Plant Ecology, 2014, 38(7): 749-756. DOI: 10.3724/SP.J.1258.2014.00070
图1 不同浓度的壳聚糖对红松幼苗中多酚类物质积累量的影响(平均值±标准误差)。*, p < 0.05; **, p < 0.01。
Fig. 1 Effects of different chitosan concentrations on the contents of polyphenols compounds in Pinus koraiensis seedlings (mean ± SE). *, p < 0.05; **, p < 0.01.
图2 不同浓度的壳聚糖对红松幼苗生长量的影响(平均值±标准误差)。*, p < 0.05; **, p < 0.01。
Fig. 2 Effects of different chitosan concentrations on the growth of Pinus koraiensis seedlings (mean ± SE). *, p < 0.05; **, p < 0.01.
图4 100 mg·L-1壳聚糖诱导红松幼苗多酚积累量的变化(平均值±标准误差)。*, p < 0.05; **, p < 0.01。
Fig. 4 Changes in polyphenols accumulation in Pinus koraiensis seedlings treated with 100 mg·L-1 chitosan (mean ± SE). *, p < 0.05; **, p < 0.01.
图5 100 mg·L-1壳聚糖诱导红松幼苗原花青素积累量的变化(平均值±标准误差)。**, p < 0.01。
Fig. 5 Changes in proanthocyanidins accumulation in Pinus koraiensis seedlings treated with 100 mg·L-1 chitosan (mean ± SE). **, p < 0.01.
图6 100 mg·L-1壳聚糖诱导红松幼苗超氧化物歧化酶(SOD)活性的变化(平均值±标准误差)。*, p < 0.05; **, p < 0.01。
Fig. 6 Changes in superoxide dismutase (SOD) activities in Pinus koraiensis seedlings treated with 100 mg·L-1 chitosan (mean ± SE). *, p < 0.05; **, p < 0.01.
图7 100 mg·L-1壳聚糖诱导红松幼苗过氧化物酶(POD)活性的变化(平均值±标准误差)。*, p < 0.05。
Fig. 7 Changes in peroxidase (POD) activities in Pinus koraiensis seedlings treated with 100 mg·L-1 chitosan (mean ± SE). *, p < 0.05.
图8 100 mg·L-1壳聚糖诱导红松幼苗过氧化氢酶(CAT)活性的变化(平均值±标准误差)。*, p < 0.05; **, p < 0.01。
Fig. 8 Changes in catalase (CAT) activities in Pinus koraiensis seedlings treated with 100 mg·L-1 chitosan (mean ± SE). *, p < 0.05; **, p < 0.01.
图9 100 mg·L-1壳聚糖诱导红松幼苗苯丙氨酸转氨酶(PAL)活性的变化(平均值±标准误差)。*, p < 0.05; **, p < 0.01。
Fig. 9 Changes in phenylalanine ammonia-lyase (PAL) activities in Pinus koraiensis seedlings treated with 100mg·L-1 chitosan (mean ± SE). *, p < 0.05; **, p < 0.01.
图10 100 mg·L-1壳聚糖诱导红松幼苗肉桂酸4-羟化酶(C4H)活性的变化(平均值±标准误差)。**, p < 0.01。
Fig. 10 Changes in cinnamate-4-hydroxylase (C4H) activities in Pinus koraiensis seedlings treated with 100 mg·L-1 chitosan (mean ± SE). **, p < 0.01.
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