分室培养装置在丛枝菌根真菌研究中的应用及其发展

doi:10.3724/SP.J.1258.2014.00120

摘要/Abstract

摘要：

重点围绕玻璃珠分室培养系统、H形分室培养系统、根排斥室培养系统、供体自养植物的双分室体外培养系统、丛枝菌根(AM)真菌与普通植物根器官的双重培养系统、AM真菌与Ri T-DNA转型根的双重单胞无菌培养系统、AM真菌与Ri T-DNA转型根双重培养的改良分室单胞培养系统等7个不同的分室培养装置, 对AM真菌的培养类型及其应用进行了系统的评述。其中, 采用玻璃珠分室培养装置易于将AM真菌与培养基质分开, 能获得大量纯净的AM真菌繁殖体, 用于研究AM真菌对矿质元素和微量元素的吸收, 具有不可替代的作用。H形分室培养系统和根排斥室(RECs)培养系统均能够获得连续的、可切断的共生菌根网络(CMNs), 可用于研究植物-植物、植物-昆虫之间化感作用产生的信息交流。供体自养植物的双分室培养系统有益于研究AM真菌对宿主植物在单作和混作条件下生长效应的影响。AM真菌与植物根器官的双重培养系统为研究AM真菌的侵染过程及生理、生化特性提供了极大的方便, 同时为纯培养研究提供了重要的理论依据。AM真菌与Ri T-DNA转型根的双重单胞无菌培养体系可以获得AM真菌纯净菌体, 是研究AM真菌遗传、生理、生化等特性的理想方法。以AM真菌与Ri T-DNA转型根的双重单胞无菌培养系统为基础, 可以在菌丝生长室置换培养基、在根室中补充适量碳源, 并多次收获AM真菌繁殖体。转型根改良双重培养系统是提高AM真菌孢子接种剂产量的有效方法。综上所述, AM真菌的分室培养系统已经取得显著进展, 为开展个体、种群、群落等不同层次的菌根生态学研究提供了依据。

关键词: 丛枝菌根真菌, 宿主植物, 菌丝体网络, 分室培养装置, 孢子

Abstract:

Arbuscular mycorrhizae (AM) are an important symbiosis between vascular plants and AM fungi in terrestrial ecosystems. Many studies have focused on their species diversity, distribution, and functions in natural habitats. However, AM fungi cannot be propagated in isolation; they need to be cultured with host plants. Thus, development of the culture method has been a hotspot in the AM research. In order to facilitate the advancement of research on AM fungi, we reviewed all the culture methods for AM fungi and their applications. Seven split-compartment cultivation systems were systematically discussed, including glass bead split-compartment culture system, two-compartment H-bridge cultivation system, root exclusion compartment culture system, in vitro mycorrhizal donor plants (MDP) culture system, dual axenic culture system, dual monoaxenic culture system of AM fungi with Ri T-DNA transformed root, and the improved split-compartment monoaxenic culture system of AM fungi with Ri T-DNA transformed root. Glass bead split-compartment culture system plays an irreplaceable role in easily separating AM fungi from the medium, hence obtaining a large quantity of axenic AM fungal propagules, which can be used for studying the absorption of mineral nutrients and trace elements. The H-shaped compartment cultivation system and root exclusion compartment culture system (RECs) can be used for obtaining continuous common mycorrhizal networks (CMNs), making it possible for the study of secondary metabolites information exchange, such as the plant-plant and plant-insect allelopathy. In vitro mycorrhizal donor plants (MDP) culture system has the advantage to study the biological effects of AM fungi on monoculture of host plants or mixed cultivation with different plant species. The dual axenic culture system facilitates the study of the infection process of AM fungi and their physiological and biochemical properties, and assists with gaining theoretical understanding on pure culture of AM fungi. Dual monoaxenic culture system of AM fungi with Ri T-DNA transformed root could be used to obtain axenic mycelium of AM fungi, and for further study of its genetic, physiological and biochemical properties. Based on dual monoaxenic culture system of AM fungi with Ri T-DNA transformed root, the medium can be replaced in the hyphal compartment and carbon source could be supplemented in the mycorrhizal compartment, and thus AM fungal propagules could be harvested continuously. The improved split-compartment monoaxenic culture system of AM fungi with Ri T-DNA transformed root is an effective method to improve the production of the inoculants with AM fungi. In a word, the different split-compartment instruments have provided effective methods for mycorrhizal research in autecology, population ecology and community ecology.

Key words: arbuscular mycorrhizae fungi, host plants, network of mycelium, split-compartment instrument, spores

王强,王茜,董梅,王晓娟,张亮,金樑. 分室培养装置在丛枝菌根真菌研究中的应用及其发展. 植物生态学报, 2014, 38(11): 1250-1260. DOI: 10.3724/SP.J.1258.2014.00120

WANG Qiang,WANG Qian,DONG Mei,WANG Xiao-Juan,ZHANG Liang,JIN Liang. Application and progress of split-compartment facility in studies of arbuscular mycorrhizal fungi. Chinese Journal of Plant Ecology, 2014, 38(11): 1250-1260. DOI: 10.3724/SP.J.1258.2014.00120

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链接本文: https://www.plant-ecology.com/CN/10.3724/SP.J.1258.2014.00120

https://www.plant-ecology.com/CN/Y2014/V38/I11/1250

图/表 4

图1 常规(A)和改进(B)的玻璃珠分室培养装置。 a和d为1 mm尼龙网, b和c为30 μm尼龙网; 将装置分隔为5个分室, 其中1和5为植物生长室, 2和4为菌根生长室, 3为AM真菌菌丝生长室。在B装置的2和4菌根生长室中装满粗河砂来代替A装置中的玻璃珠。

Fig. 1 Traditional (A) and modified (B) glass bead split-compartment culture system. a and d are nylon mesh screens of 1 mm, and b and c are nylon mesh screens of 30 μm; each container was separated into five compartments which 1 and 5 are plant growing compartments, 2 and 4 are mycorrhizal growing compartments, and 3 is the AM fungal hyphae growing compartment. The 2 and 4 compartments in B filled with coarse river sands instead of glass beads in A.

图2 H形装置结构构件图(改绘自Barto et al., 2011)。 1, 30 μm尼龙网; 2, 人工化感物质注入孔; 3, 供体植物; 4, 穿孔的钢板网; 5, 30 μm尼龙网; 6, 受体植物。30 μm尼龙网和穿孔钢板网将装置分隔为两个相同的分室。

Fig. 2 The two-compartment H-bridge cultivation system (Mo- dified from Barto et al., 2011). 1, nylon mesh screens of 30 μm; 2, injection hole for artificial allelochemicals; 3, donor plants; 4, perforated steel plates; 5, nylon mesh screens of 30 μm; 6, receiver plants. The device was separated by the nylon mesh screens of 30 μm and the perforated steel plates into two identical compartments.

图3 根排斥室(RECs)培养系统示意图(改绘自Barto et al., 2011 和Babikova et al., 2013) 。 A, 根外菌丝进入REC。B, 旋转REC切断了共生菌根网络(CMNs)与RECs的联系。1, 可以产生化感物质的植物; 2, 根排斥室; 3, 根排斥室内PDMS微型管; 4, 根外菌丝; 5, 植物根系; 6, 根排斥室外PDMS微型管, 双向箭头代表旋转根排斥室。

Fig. 3 The root exclusion compartment (RECs) culture system (Modified from Barto et al., 2011 and Babikova et al., 2013). A, Extra-radical mycelium entering into REC. B, Spinning out REC to cut the communication between common mycorrhizal networks (CMNs) and RECs. 1, plants which can produce allelochemicals; 2, RECs; 3, intra-REC PDMS tubing; 4, external hyphae; 5, plant roots; 6, extra-REC PDMS tubing, and the doubled sided arrow stands for rotary RECs.

图4 供体自养植物双分室体外培养系统(改绘自Derelle et al., 2012)。 HC, 根外菌丝生长室; RC, 根系生长室。粗线代表供体自养植物的根系, 细线代表AM真菌根外菌丝。

Fig. 4 In vitro mycorrhizal donor plants culture system (Modified from Derelle et al., 2012). HC, external hyphae growing compartment; RC, root growing compartment; The thick line stands for roots of donor autotrophic plants, and the thin line stands for external hyphae.

参考文献 64

1	Abdel Latef AAH, He CX ( 2011). Effect of arbuscular mycorrhizal fungi on growth, mineral nutrition, antioxidant enzymes activity and fruit yield of tomato grown under salinity stress. Scientia Horticulturae, 127, 228-233. DOI URL
2	Amir H, Lagrange A, Hassaïne N, Cavaloc Y ( 2013). Arbuscular mycorrhizal fungi from New Caledonian ultramafic soils improve tolerance to nickel of endemic plant species. Mycorrhiza, 23, 585-595. DOI URL
3	Babikova Z, Gilbert L, Bruce TJA, Birkett M, Caulfield JC, Woodcock C, Johnson D ( 2013). Underground signals carried through common mycelial networks warn neighbouring plants of aphid attack. Ecology Letters, 16, 835-843. DOI URL
4	Babikova Z, Johnson D, Bruce T, Pickett J, Gilbert L ( 2014). Underground allies: How and why do mycelial networks help plants defend themselves? BioEssays, 36, 21-26. DOI URL
5	Bago B, Azcón-Aguilar C, Piché Y ( 1998). Architecture and developmental dynamics of the external mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown under monoxenic conditions. Mycologia, 90, 52-62. DOI URL
6	Bais HP, Vepachedu R, Gilroy S, Callaway RM, Vivanco JM ( 2003). Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science, 301, 1377-1380. DOI URL
7	Barto EK, Hilker M, Müller F, Mohney BK, Weidenhamer JD, Rillig MC ( 2011). The fungal fast lane: common mycorrhizal networks extend bioactive zones of allelochemicals in soils. PLoS ONE, 6, e27195. DOI URL
8	Barto EK, Weidenhamer JD, Cipollini D, Rillig MC ( 2012). Fungal superhighways: Do common mycorrhizal networks enhance below ground communication? Trends in Plant Science, 17, 633-637. DOI URL
9	Bécard G, Fortin JA ( 1998). Early events of vesicular-arbuscular mycorrhizal formation on Ri T-DNA transformed roots. New Phytologist, 108, 211-218. DOI URL
10	Behie SW, Bidochka MJ ( 2014). Ubiquity of insect-derived nitrogen transfer to plants by endophytic insect-pathogenic fungi: an additional branch of the soil nitrogen cycle. Applied and Environmental Microbiology, 80, 1553-1560. DOI URL
11	Bi YL, Wang HG, Li XL ( 1999). The morphological characteristics of AM symbiosis between arbuscular mycorrhizal fungus and transformed Ri T-DNA carrot roots. Mycosystema, 18, 159-163. (in Chinese with English abstract)
12	[ 毕银丽, 汪洪钢, 李晓林 ( 1999). 丛枝菌根真菌与转移Ri T-DNA胡萝卜根器官双重培养的形态学研究. 菌物系统, 18, 159-163.]
	Bi YL, Wang HG, Li XL ( 2000). Establishment of dual culture for arbuscular mycorrhiza and formation of hyphosphere. Mycosystema, 19, 517-521. (in Chinese with English abstract)
13	[ 毕银丽, 汪洪钢, 李晓林 ( 2000). 丛枝菌根的双重培养方法及其菌丝际的建立. 菌物系统, 19, 517-521.]
	Blair AC, Hanson BD, Brunk GR, Marrs RA, Westra P, Nissen SJ, Hufbauer RA ( 2005). New techniques and findings in the study of a candidate allelochemical implicated in invasion success. Ecology Letters, 8, 1039-1047.
14	Callaway RM, Cipollini D, Barto K, Thelen GC, Hallett SG, Prati D, Klironomos J ( 2008). Novel weapons: invasive plant suppresses fungal mutualists in America but not in its native Europe. Ecology, 89, 1043-1055. DOI URL
15	Chabot S, Bel-Rhlid R, Chênevert R, Piché Y ( 1992). Hyphal growth promotion in vitro of the VA mycorrhizal fungus, Gigaspora margarita Becker & Hall, by the activity of structurally specific flavonoid compounds under CO₂- enriched conditions. New Phytologist, 122, 461-467. DOI URL
16	Chen BD, Christie P, Li XL ( 2001). A modified glass bead compartment cultivation system for studies on nutrient and trace metal uptake by arbuscular mycorrhiza. Chemosphere, 42, 185-192. DOI URL
17	Chen SC, Jin WJ, Liu AR, Zhang SJ, Liu DL, Wang FH, Lin XM, He CX ( 2013). Arbuscular mycorrhizal fungi (AMF) increase growth and secondary metabolism in cucumber subjected to low temperature stress. Scientia Horticulturae, 160, 222-229. DOI URL
18	Derelle D, Declerck S, Genet P, Dajoz I, van Aarle IM ( 2012). Association of highly and weakly mycorrhizal seedlings can promote the extra-and intraradical development of a common mycorrhizal network. FEMS Microbiology Ecology, 79, 251-259. DOI URL
19	Diop TA, Plenchette C, Strulu DG ( 1994a). In vitro culture of sheared mycorrhizal roots. Symbiosis, 17, 217-227.
20	Diop TA, Plenchette C, Strullu DG ( 1994b). Dual axenic culture of sheared-root inocula of vesicular-arbuscular mycorrhizal fungi associated with tomato roots. Mycorrhiza, 5, 17-22. DOI URL
21	Douds Jr DD ( 1997). A procedure for the establishment of Glomus mosseae in dual culture with Ri T-DNA transformed carrot roots. Mycorrhiza, 7, 57-61. DOI URL
22	Douds Jr DD ( 2002). Increased spore production by Glomus intraradices in the split-plate monoxenic culture system by repeated harvest, gel replacement, and resupply of glucose to the mycorrhiza. Mycorrhiza, 12, 163-167. DOI URL
23	Egerton-Warburton LM, Querejeta JI, Allen MF ( 2007). Common mycorrhizal networks provide a potential pathway for the transfer of hydraulically lifted water between plants. Journal of Experimental Botany, 58, 1473-1483. DOI URL
24	Gadkar V, Driver JD, Rillig MC ( 2006). A novel in vitro cultivation system to produce and isolate soluble factors released from hyphae of arbuscular mycorrhizal fungi. Biotechnology Letters, 28, 1071-1076. DOI URL
25	Göhre V, Paszkowski U ( 2006). Contribution of the arbuscular mycorrhizal symbiosis to heavy metal phytoremediation. Planta, 223, 1115-1122.
26	Grunwald U, Guo WB, Fischer K, Isayenkov S, Ludwig-Müler J, Hause B, Yan XL, Küter H, Franken P ( 2009). Overlapping expression patterns and differential transcript levels of phosphate transporter genes in arbuscular mycorrhizal, Pi-fertilised and phytohormone-treated Medicago truncatula roots. Planta, 229, 1023-1034. DOI URL
27	Gyuricza V, Thiry Y, Wannijn J, Declerck S, Dupré de Boulois H ( 2010). Radiocesium transfer between Medicago truncatula plants via a common mycorrhizal network. Environmental Microbiology, 12, 2180-2189.
28	Hattingh MJ, Gray LE, Gerdemann WJ ( 1973). Uptake and translocation of ³²P-labelled phosphate to onion roots by endomycorrhizal fungi . Soil Science, 116, 383-387. DOI URL
29	Karandashov V, Kuzovkina I, Hawkins H J, George E ( 2000). Growth and sporulation of the arbuscular mycorrhizal fungus Glomus caledonium in dual culture with transformed carrot roots. Mycorrhiza, 10, 23-28. DOI URL
30	Kaya C, Ashraf M, Sonmez O, Aydemir S, Tuna AL, Cullu MA ( 2009). The influence of arbuscular mycorrhizal colonisation on key growth parameters and fruit yield of pepper plants grown at high salinity. Scientia Horticulturae, 121, 1-6. DOI URL
31	Kaya C, Higgs D, Kirnak H, Tas I ( 2003). Mycorrhizal colonisation improves fruit yield and water use efficiency in watermelon ( Citrullus lanatus Thunb.) grown under well-watered and water-stressed conditions. Plant and Soil, 253, 287-292. DOI URL
32	Li XL, George E, Marschner H ( 1991). Extension of the phosphorus depletion zone in VA-mycorrhiza whiter clover in a calcareous soil. Plant and Soil, 136, 41-48. DOI URL
33	Liu Q, Parsons AJ, Xue H, Jones CS, Rasmussen S ( 2013). Functional characterisation and transcript analysis of an alkaline phosphatase from the arbuscular mycorrhizal fungus Funneliformis mosseae. Fungal Genetics and Biology, 54, 52-59. DOI URL
34	Maldonado-Mendoza IE, Dewbre GR, Harrison MJ ( 2001). A phosphate transporter gene from the extraradical mycelium of an arbuscular mycorrhizal fungus Glomus intraradices is regulated in response to phosphate in the environment. Molecular Plant-Microbe Interactions, 14, 1140-1148. DOI URL
35	Martínez-Medina A, Pascual JA, Pérez-Alfocea F, Albacete A, Roldán A ( 2010). Trichoderma harzianum and Glomus intraradices modify the hormone disruption induced by Fusarium oxysporum infection in melon plants. Phytopathology, 100, 682-688. DOI URL
36	Meding SM, Zasoski RJ ( 2008). Hyphal-mediated transfer of nitrate, arsenic, cesium, rubidium, and strontium between arbuscular mycorrhizal forbs and grasses from a California oak woodland. Soil Biology & Biochemistry, 40, 126-134. DOI URL
37	Mohney BK, Matz T, LaMoreaux J, Wilcox DS, Gimsing AL, Mayer P, Weidenhamer JD ( 2009). In situ silicone tube microextraction: a new method for undisturbed sampling of root-exuded thiophenes from marigold ( Tagetes erecta L.) in soil. Journal of Chemical Ecology, 35, 1279-1287. DOI URL
38	Mosse B, Hepper C ( 1975). Vesicular-arbuscular mycorrhiza infections in root organ cultures. Physiological Plant Pathology, 5, 215-223. DOI URL
39	Mugnier J, Mosse B ( 1987). Vesicular-arbuscular mycorrhizal infection in transformed Ri T-DNA roots grown axenically. Phytopathology, 77, 1045-1050. DOI URL
40	Nagahashi G, Douds Jr DD ( 2000). Partial separation of root exudate components and their effects upon the growth of germinated spores of AM fungi. Mycological Research, 104, 1453-1464. DOI URL
41	Navarro A, Elia A, Conversa G, Campi P, Mastrorilli M ( 2012). Potted mycorrhizal carnation plants and saline stress: growth, quality and nutritional plant responses. Scientia Horticulturae, 140, 131-139. DOI URL
42	Ooki A, Yokouchi Y ( 2008). Development of a silicone membrane tube equilibrator for measuring partial pressures of volatile organic compounds in natural water. Environmental Science & Technology, 42, 5706-5711. DOI URL
43	Plenchette C, Declerck S, Diop TA, Strullu DG ( 1996). Infectivity of monoaxenic subcultures of the arbuscular mycorrhizal fungus Glomus versiforme associated with Ri-T-DNA-transformed carrot root. Applied Microbiology and Biotechnology, 46, 545-548. DOI URL
44	Redecker D, Thierfelder H, Werner D ( 1995). A new cultivation system for arbuscular mycorrhizal fungi on glass beads. Angewandte Botanik, 69, 189-191.
45	Ren L, Lou Y, Zhang N, Zhu X, Hao W, Sun S, Xu G ( 2013). Role of arbuscular mycorrhizal network in carbon and phosphorus transfer between plants. Biology and Fertility of Soils, 49, 3-11. DOI URL
46	Schüßler A, Walker C (2010). The Glomeromycota: a species list with new families and new genera. http://schuessler.userweb.mwn.de/amphylo/Schuessler&Walker2010_Glomeromycota.pdf . Cited: April 2014.
47	Smith SE, Read DJ (2008). Mycorrhizal Symbiosis. 3rd edn. Academic Press, New York.
48	Soliman AS, Shanan NT, Massoud ON, Swelim DM ( 2014). Improving salinity tolerance of Acacia saligna(Labill.) plant by arbuscular mycorrhizal fungi and Rhizobium inoculation. African Journal of Biotechnology, 11, 1259-1266. DOI URL
49	Song YY, Cao M, Xie LJ, Liang XT, Zeng RS, Su YJ, Huang JH, Wang RL, Luo SM ( 2011). Induction of DIMBOA accumulation and systemic defense responses as mechanism of enhanced resistance of mycorrhizal corn ( Zea mays L.) to sheath blight. Mycorrhiza, 21, 721-731. DOI URL
50	Song YY, Ye M, Li C, He X, Zhu-Salzman K, Wang RL, Zeng RS ( 2014). Hijacking common mycorrhizal networks for herbivore-induced defence signal transfer between tomato plants. Scientific Reports, 4, doi: 10.1038/srep03915.
51	Song YY, Zeng RS, Xu JF, Li J, Shen X, Yihdego WG ( 2010). Interplant communication of tomato plants through underground commonmycorrhizal networks. PLoS ONE, 5, e13324. DOI URL
52	St-Arnaud M, Hamel C, Vimard B, Caron M, Fortin JA ( 1995). Altered growth of Fusarium oxysporum f. sp. Chrysanthemi in an in vitro dual culture system with the vesicular- arbuscular mucorrhizal fungus Glomus intraradices growing on Daucus carota trasformed root. Mycorrhiza, 5, 431-438.
53	St-Arnaud M, Hamel C, Vimard B, Caron M, Fortin JA ( 1996). Enhanced hyphal growth and spore production of the arbuscular mycorrhizal fungus Glomus intraradices in an in vitro system in the absence of host roots. Mycological Research, 100, 328-332. DOI URL
54	Tiwari P, Adholeya A ( 2002). In vitro co-culture of two AMF isolates Gigaspora margarita and Glomus intraradices on Ri T-DNA transformed roots. FEMS Microbiology Letters, 206, 39-43. DOI URL
55	Ulrich H, Katharina J, Hermann B ( 2002). Towards growth of arbscular mycorrhizal fungi independent of a plant host. Applied and Environmental Microbiology, 68, 1919-1924. DOI URL
56	Vági P, Knapp D G, Kósa A, Seress D, Horváth ÁN, Kovács GM ( 2014). Simultaneous specific in planta visualization of root-colonizing fungi using fluorescence in situ hybridization (FISH). Mycorrhiza, 24, 259-266. DOI URL
57	Vandenhove H, van Hees M, Vandecasteele C ( 2000). Potential side effects of ammonium-ferric-hexacyano-ferrate application: enhanced radiostrontium transfer and free cyanide release. Journal of Environmental Radioactivity, 47, 149-155. DOI URL
58	Voets L, de la Providencia IE, Fernandez K, IJdo M, Cranenbrouck S, Declerck S ( 2009). Extraradical mycelium network of arbuscular mycorrhizal fungi allows fast colonization of seedlings under in vitro conditions. Mycorrhiza, 19, 347-356. DOI URL
59	Voets L, Goubau I, Olsson PA, Merckx R, Declerck S ( 2008). Absence of carbon transfer between Medicago truncatula plants linked by a mycorrhizal network, demonstrated in an experimental microcosm. FEMS Microbiology Ecology, 65, 350-360. DOI URL
60	Vos C, Claerhout S, Mkandawire R, Panis B, de Waele D, Elsen A ( 2011). Arbuscular mycorrhizal fungi reduce root-knot nematode penetration through altered root exudation of their host. Plant and Soil, 354, 335-345. DOI URL
61	Walder F, Niemann H, Natarajan M, Lehmann MF, Boller T, Wiemken A ( 2012). Mycorrhizal networks: common goods of plants shared under unequal terms of trade. Plant Physiology, 159, 789-797. DOI URL
62	Wang HG, Wu GY, Li HQ ( 1990). The penetration of vesicular arbuscular mycorrhizal fungi and rhizobium to the root organ of Phaseolus aureus Roxb. Microbiology, 17(4), 193-195. (in Chinese with English abstract)
63	[ 汪洪钢, 吴观以, 李慧荃 ( 1990). VA菌根真菌与根瘤菌对离体绿豆根器官的侵染. 微生物学通报, 17(4), 193-195.] DOI URL
	Wu QS, Xia RX, Zou YN ( 2008). Improved soil structure and citrus growth after inoculation with three arbuscular mycorrhizal fungi under drought stress. European Journal of Soil Biology, 44, 122-128.
64	Xiao TJ, Yang QS, Ran W, Xu GH, Shen QR ( 2010). Effect of inoculation with arbuscular mycorrhizal fungus on nitrogen and phosphorus utilization in upland rice-mungbean intercropping system. Agricultural Sciences in China, 9, 528-535. DOI URL

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