植物生态学报 ›› 2014, Vol. 38 ›› Issue (9): 949-958.DOI: 10.3724/SP.J.1258.2014.00089

• 研究论文 • 上一篇    下一篇

荒漠北沙柳根系丛枝菌根真菌和黑隔内生真菌定殖状况

闫姣,贺学礼(),张亚娟,许伟,张娟,赵丽莉   

  1. 河北大学生命科学学院, 河北保定 071002
  • 收稿日期:2014-03-13 接受日期:2014-07-02 出版日期:2014-09-01 发布日期:2014-09-22
  • 通讯作者: 贺学礼
  • 基金资助:
    国家自然科学基金(31270460);河北省自然科学基金(C2014201060)

Colonization of arbuscular mycorrhizal fungi and dark septate endophytes in roots of desert Salix psammophila

YAN Jiao,HE Xue-Li(),ZHANG Ya-Juan,XU Wei,ZHANG Juan,ZHAO Li-Li   

  1. College of Life Sciences, Hebei University, Baoding, Hebei 071002, China
  • Received:2014-03-13 Accepted:2014-07-02 Online:2014-09-01 Published:2014-09-22
  • Contact: HE Xue-Li

摘要:

为利用土壤共生真菌资源促进荒漠植被恢复和生态重建, 分别于2013年6月、8月和10月, 从内蒙古元上都地区采集北沙柳(Salix psammophila)根围0-10、10-20、20-30、30-40和40-50 cm共5个土层的土壤样品, 系统研究了丛枝菌根真菌(AMF)和黑隔内生真菌(DSE)的时空分布及其与土壤因子的相关性。结果表明: AMF和DSE的平均定殖率分别为77%和84%, 说明北沙柳根系能与这两类真菌形成良好的共生关系。AMF和DSE的分布和定殖具有明显的时空异质性, 并与土壤因子密切相关。AMF和DSE的平均定殖率均表现为10月> 8月> 6月。土壤深度对AMF和DSE的定殖率有显著影响, AMF和DSE定殖率的最大值分别在0-20 cm和0-10 cm土层。双因子方差分析表明, 月份和土层对AMF和DSE的定殖率以及土壤因子具有显著的交互效应。主成分分析表明, 土壤湿度、pH值、碱性磷酸酶、易提取球囊霉素是内蒙古荒漠环境中AMF和DSE定殖的主要影响因子。

关键词: 丛枝菌根真菌, 黑隔内生真菌, 生态分布, 内蒙古荒漠, 北沙柳

Abstract:

Aims Salix psammophila is an excellent shrub for afforestation and sand dune fixation and is mainly distributes in the arid zones in North China. The objective of this study was to determine the colonization of arbuscular mycorrhizal fungi (AMF) and dark septate endophytes (DSE), and the relationships among AMF, DSE and soil factors in the rhizosphere of S. psammophila, in order to provide information guiding the recovery of desert vegetation and ecological reconstruction.
Methods Soil and root samples were collected from different soil layers (0-10, 10-20, 20-30, 30-40 and 40-50 cm) in the rhizosphere of S. psammophila in June, August and October 2013 at Yuan Shangdu in Nei Mongol. The colonization and distribution of AMF and DSE were assessed, and the relationships among AMF, DSE and soil factors were determined and tested by variance analysis, correlation analysis and principal component analysis.
Important findings The roots of S. psammophila could be highly infected by AMF and DSE, and the average colonization of AMF and DSE were 77% and 84%, respectively. Sampling time and soil depth significantly influenced the AMF and DSE colonization. The maximum colonization of AMF was found in the 0-20 cm soil layer and DSE in the 0-10 cm soil layer, respectively, and the effects of sampling time on colonization were in the order of October > August > June. Two-way ANOVA showed that sampling time and soil layer had significant interactive effects on the colonization and distribution of AMF and DSE as well as soil factors. Principal component analysis showed that soil humidity, pH value, alkaline phosphatase and easily extractable glomalin were the main factors of AMF and DSE colonization in desert environments.

Key words: arbuscular mycorrhizal fungi, dark septate endophytes, ecological distribution, Nei Mongol desert, Salix psammophila